Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Changes in metabolic rate and hematological parameters of black rockfish (Sebastes schlegeli) in relation to temperature and hypoxia

수온과 저산소에 따른 조피볼락(Sebastes schlegeli)의 호흡대사와 혈액성상의 변화

  • Kim, Heung-Yun (Department of Aqualife Medicine, Chonnam National University)
  • 김흥윤 (전남대학교 수산생명의학과)
  • Received : 2021.11.29
  • Accepted : 2021.12.08
  • Published : 2021.12.31
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Abstract

Experiments were performed to investigate changes in metabolic rate (MO2), critical oxygen saturation (Scrit) and hematological parameters of black rockfish, Sebastes schlegeli exposed to hypoxia at 15, 20 and 25℃. The MO2 was measured at an interval of 10 min using intermittent-flow respirometry. The normoxic standard metabolic rate (SMR) was 116.5±5.5, 188.6±4.2 and 237.4±6.8 mg O2/kg/hr, and Scrit was 22.1±1.2, 30.6±1.5 and 41.9±1.4% air saturation at 15, 20 and 25℃, respectively. Q10 values were 2.62 between 15 and 20℃, 1.58 between 20 and 25℃, and 2.04 over the full temperature range. In the investigation of blood (hematocrit and hemoglobin) and biochemical parameters (plasma cortisol, glucose, electrolyte and osmolality), the rockfish were subjected to Scrit for each temperature during 4 hr. All of hematological parameters of the rockfish exposed to hypoxic water were significantly higher than those of normoxic control. Moreover, blood and biochemical parameters of the rockfish maintained to normoxic water showed the tendency of increase with temperature, and were significantly higher at 25C. As a result of this experiment, it was found that physiological stress due to hypoxia increased at high temperature.

본 연구는 수온 15, 20 및 25℃에서 조피볼락(Sebastes schlegeli)을 점진적인 저산소 조건에 노출시켜 호흡 대사율이 표준대사율(SMR) 이하로 감소하는 산소포화도, Scrit를 평가하고, 수온별 Scrit 에 4시간 노출 후 혈액 성상의 변화를 조사하기 위하여 실시하였다. 수온 15, 20, 25℃에서 normoxic SMR은 각각 116.5±5.5, 188.6±4.2 및 237.4±6.8 mg O2/kg/hr이었고, 호흡계수(Q10)는 15-20℃ 구간에서는 2.62, 20-25℃에서는 1.58, 그리고 전 수온 범위의 15-25℃에서는 2.04였다. 수온 15, 20 및 25℃에서 Scrit는 각각 22.1±1.2, 30.6±1.5 및 41.9±1.4 %였다. 수온별 Scrit에 4시간 노출 후 혈액 Ht와 Hb 및 혈장 cortisol, glucose, 전해질(Na+, K+, Cl-)과 삼투질 농도는 모든 수온에서 normoxic water의 대조구에 비하여 유의하게 높았다. 수온 15, 20, 25℃의 normoxic water에서는 Ht, Hb 및 혈장 glucose 농도는 15℃에 비하여 20℃와 25℃에서, 혈장 cortisol, 전해질 및 삼투질 농도는 25℃에서 유의하게 높았다. 수온별 Scrit의 저산소에 노출된 조피볼락은 수온이 높을수록 저산소에 의한 어체의 생리적 스트레스는 가중되는 것으로 나타났다.

Keywords

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