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http://dx.doi.org/10.5352/JLS.2007.17.1.143

Inhibition effect of neuronal death by Scutellaria baicalensis GEORGI Water-Extract in a Hypoxic Model of Cultured Rat Cortical Cells  

Kim, Wan-Sik (Dept. of Oriental Medicine, Dongguk University)
Jung, Seung-Hyun (Dept. of Oriental Medicine, Dongguk University)
Shin, Gil-Jo (Dept. of Oriental Medicine, Dongguk University)
Moon, Il-Soo (Department of Anatomy, Dongguk University)
Lee, Won-Chol (Dept. of Oriental Medicine, Dongguk University)
Publication Information
Journal of Life Science / v.17, no.1, 2007 , pp. 143-149 More about this Journal
Abstract
Scutellaria baicalensis GEORGI(SB) is used in oriental medicine for the treatment of incipient strokes. Although it has been reported that SB is neuroprotective in a hypoxia model, its mechanism is poorly understood. Here, we investigated the effect of SB on the modulation of retinoic acid receptor a (RARa). Rat cerebrocortical cells were grown in neurobasal medium. On DIV12 cells were treated with SB $(20{\mu}g/ml)$ and given a hypoxic shock $(2%\;O_2/5%\;CO_2,\;3hr)$ on DIV14. In situ hybridization using cRNA probe revealed that RARa mRNA punctae are distributed, in addition to nucleus, throughout neuronal dendrites, where SB upregulated its density by 69.8% (p=0.001) and 129.8% (p=0.001) in both normoxia and hypoxia, respectively. At the protein level, SB upregulated RARa in the neuronal soma by 78.8% (p=0.004) and 23.6% (p=0.001) in both normoxia and hypoxia, respectively. These results indicate that SB upregulates RARa in both normoxia and hypoxia, which might contribute to the neuroprotection.
Keywords
rat cortical cell culture; hypoxia; IC; ISH; RARa; Scutellaria haicalensis GEORGI;
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  • Reference
1 Kim. H. G. 2000. Pharmacology of Korea, pp. 222-227, Korea medical book publisher
2 Semenza, G. L., P. H. Roth, H. M. Fang and G. L. Wang. 1994. Transcriptional regulation of genes encoding glycolytic enzymes by hypoxia-inducible factor 1. J. Biol. Chem. 269, 23757-23763
3 Semenza, G. L., B. H. Jiang, S. W. Leung, R. Passantino, J. P. Concordet, P. Maire and A. Giallongo. 1996. Hypoxia response elements in the aldolase A, enolase 1, and lactate dehydrogenase A gene promoters contain essential binding sites for hypoxia-inducible factor 1. J. Biol. Chem. 271, 32529-32537   DOI   ScienceOn
4 Wuarin, L. and N. Sidell. 1991. Differential susceptibilities of spinal cord neurons to retinoic acid-induced survival and differentiation. Dev. Biol. 144, 429-435   DOI   ScienceOn
5 Wuarin, L., N. Sidell, J. de Vellis. 1990. Retinoids increase perinatal spinal cord neuronal survival and astroglial differentiation. Int. J. Dev Neurosci. 8, 317-326   DOI   ScienceOn
6 Yang, Y., Ms Vacchio and J. D. Ashwell. 1993. 9-cis-retinoic acid inhibits activation-driven T-cell apoptosis: implications for retinoid X receptor involvement in thymocyte development. Proc. Natl. Acad. Sci. USA. 90, 6170-6174   DOI   ScienceOn
7 Yoon, K. S. 2004. Effects of Scutellaria baicalensis GEORGI(黃芩) on the Modulation of ROS, MMP, in a Hypoxic Model of Cultured Rat Cortical Cells. Dongguk graduate school
8 Lee S. J. 1995. Bon-cho-kang-mok, pp. 580, Daesung Medical press, Seoul
9 Wang-Ang. 1989. Bon-cho-bi-yo, pp. 44-45, Komoonsa, Seoul
10 Ji H. J., S. I. Lee and D. G. Ann. 1998. Hanyak-Gyugyuk-Juhae, pp. 686-688, Korean medical index com. Seoul
11 Heo J. 1999. Dong-Eui-Bo-Cam, pp. 367, 1024, 1937, 1938, Bubinbooks, Seoul
12 Hwang D. Y. 1989. Bang Yak Hap Pyun, pp. 120-130, Namsandang, Seoul
13 Lee J. M. 1992. Dongyi-Suse-Bowon, Yeogang book publisher
14 Kroemer, G., B. Dallaporta and Resche-Rigon M. 1998. The mitochondrial death/life regulator in apoptosis and necrosis. Ann. Rev. Physiology. 60, 619-642   DOI   ScienceOn
15 Lee J. H., S. J. Shin and Y. Moon. 1998. Effect of Scutellaria baicalensis Extract on the Immune Functions, Microbial Growth and Mutagenicity. The Korean Association of Immunobiologists 20, 343-348
16 Levy, A.P., N.S. Levy, S. Wegner and M. A. Goldberg. 1995. Transcriptional regulation of the rat vascular endothelial growth factor gene by hypoxia. J. Biol Chem. 270, 13333-13340   DOI   ScienceOn
17 Mangelsdorf, D. J., S. A. Kliewer, A. Kakizuka. K. Umesono and R. M. Evans. 1993. Retinoid receptors. Recent Prog. Horm. Res. 48, 99-121
18 Prabhakar, N. R., J. L. Overholt. 2001. Cellular mechanisms of oxygen sensing at the carotid body: heme proteins and ion channels. Resp. Physiol. 122, 209-221
19 Nieminen, A. L. 2003. Apoptosis and necrosis in health and disease: role of mitochondria. Int Rev Cytol. 224, 29-55   DOI   ScienceOn
20 Nilsson, G. E. 2001. Surviving anoxia with the brain turned on. News Physiol Sci. 16, 217-221
21 Prabhaker, N. R. and J. L. Overholt. 2000. Cellular mechanisms of oxygen sensing at the carotid body: heme proteins and ion channels. Resp. Physiol. 122, 209-221   DOI   ScienceOn
22 Rossi, D.J., T. Oshima and D. Attwell. 2000. Glutamate release in severe brain ischemia is mainly by reversed uptake. Nature. 403, 316-321   DOI   ScienceOn
23 Semenza, G. L. 1999. Regulation of mammalian $O_2$ homeostasis by hypoxia-inducible factor 1. Ann. Rev. Cell Dev. Biol. 15, 551-578   DOI   ScienceOn
24 Semenza, G. L. 1998. Hypoxia-inducible factor 1: master regulator of O2 homeostasis. Curr Opin. Genet. Dev. 8, 588-594   DOI   ScienceOn
25 Erecinska, M. and I. A. Silver. 2001. Tissue oxygenation and brain sensitivity to hypoxia. Resp Physiol. 128, 263-276   DOI   ScienceOn
26 Haddad, J. J. and S. C. Land. 2000. $O_2$-evoked regulation of HIF-1a and $NF_{\kappa}B$ in perinatal lung epithelium requires glutathione biosynthesis. Am. J. Physiol, Lung Cell Mol. Physiol. 278, L492-L503
27 Hansen, A. 1985. Extracellular potassium concentration in juvenile and adult brain cortex during anoxia. Acta Physiol. Scand. 99, 412-428   DOI   ScienceOn
28 Huang, F. J., Y. C. Hsu, H. Y. Kang, S. Y. Chang, Y. D. Hsuuw and K. E. Huang. 2005. Effects of retinoic acid on the inner cell mass in mouse blastocysts. Fertil Steril, 83, 238-242   DOI   ScienceOn
29 Hochachka, P. W. and P. Lutz. 2001. Mechanism, origin, and evolution of anoxia tolerance in animals. Comp. Biochem. Physiol. B. 130, 435-459   DOI   ScienceOn
30 Huang, F. J., C. C. Shen, S. Y. Chang, T. C. Wu and Y. D. Hsuuw. 2003. Retinoic acid decreases the viability of mouse blastocysts in vitro. Hum. Reprod. 18, 130-136   DOI   ScienceOn
31 Kim, G. H., S. H. Jung, J. W. Kim, H. S. Eom, S. H, Jung, G. J. Shin, W. C. Lee and I. S. Moon. 2003. The effect of Scutellaria baicalensis GEORGI Water-Extract in a Hypoxic Model of Cultured Rat Cortical Cells. The Journal of Korean Oriental Internal Medicine. 3, 396-405
32 Kim, S. B., 2003. Effects of Scutellaria baicalensis Georgi on the gene expression in a hypoxic model of cultured rat cortical cells. Dongguk graduate school
33 Knickerbocker, D. L. and P. L. Lutz. 2001. Slow ATP loss and the defense of ion homeostasis in the anoxic frog brain. J. Exp. Biol. 204, 3547-3551
34 Krieglstein, J. 1990. Tharmacology and drug therapy of cerebral ischemia. pp347-371. In Schurr, A and B. M. Rigor (eds), Cerebral ischemia and resuscitation, Boca Raton CRC
35 Altucci L., A. Rossin, W. Raffelsberger, A. Reitmair, C. Chomienne and H. Gronemeyer. 2001. Retinoic acid-induced apoptosis in leukemia cells is mediated by paracrine action of tumor-selective death ligand TRAIL, Nature Medicine 7, 680-686   DOI   ScienceOn
36 Chambon, P. 1996. A decade of molecular biology of retinoic acid receptors. FASEB J. 10, 940-954   DOI
37 Bickler, P. E., P.H. Donohoe. 2002. Adaptive responses of vertebrate neurons to hypoxia. J. Exp Biol. 205, 3579-3586
38 Bissonnette, R. P., T. Brunner, S. B. Lazarchik. N. J. Yoo, M. F. Boehm, D. R. Green and R. A. Heyman. 1995. 9-cis retinoic acid inhibition of activation-induced apoptosis is mediated via regulation of fas ligand and requires retinoic acid receptor and retinoid X receptor activation. Mol. Cell. Biol. 15, 5576-5585   DOI
39 Brewer, G. J., J. R. Torricelli, E. K. Evege and P. J. Price. 1993. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J. Neurosci. Res. 35, 567-576   DOI   ScienceOn
40 Cheng Q., H. H. Lee, Y. Li, T. P. Parks and G. Cheng. 2000. Upregulation of Bcl-x and Bfl-1 as a potential mechanism of chemoresistance, which can be overcome by NF-kappaB inhibition, Oncogene 19, 4936-4940   DOI   ScienceOn
41 Clarke N., A. M. Jimenez-Lara, E. Voltz and H. Gronemeyer. 2004. Tumor suppressor IRF-1 mediates retinoid and interferon anticancer signaling to death ligand TRAIL, EMBO J. 23, 3051-3060   DOI   ScienceOn
42 Ebert, B. L., J. D. Firth and P. J. Ratcliffe. 1995. Hypoxia and mitochondrial inhibitors regulate expression of glucose transporter-1 via distinct Cis-acting sequences. J. Biol. Chem. 270, 29083-29089   DOI
43 Yin W., W. Raffelsberger and H. Gronemeyer. 2005. Retinoic acid determines life span of leukemic cells by inducing antagonistic apoptosis-regulatory programs. Int. J. Biochem. Cell Biol. Aug;37(8), 1696-708   DOI   ScienceOn
44 Lee, B. C., H. L. Kang, Y. O. Kim, S. Y. Kim, D. K. Ahn, H. K. Park and H. C. Kim. 1999. Neuroprotective Effects of Scutellariae Radix on the Brain Ischemia induced by Four - Vessel Occlusion in Rats. The Korea Association of Herbology 14, 89-96   과학기술학회마을
45 Goldberg, M. A, S. P. Dunning and H. F. Bunn, 1988. Regulation of the erythropoietin gene: evidence that the oxygen sensor is a heme protein. Science. 242, 1412-1415   DOI