• Applied Biological Chemistry
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• 제37권2호
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• pp.85-93
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• 1994

연속식 2단계 막(MWCO 10,000, MWCO 5,000)반응기를 이용하여 어피젤라틴 가수분해물을 제조하여 그 가수분해물의 분자량, 아미노산조성 및 기능성에 대하여 검토하였다. 1단계 젤라틴 가수분해물의 분자량은 $8{\sim}10\;KDa$ 및 $4.5{\sim}6.5\;KDa$이 주종을 이루었으며, 2단계 가수분해물의 분자량은 $2{\sim}6\;KDa$ 및 2 KDa 이하의 저분자 펩타이드도 존재하였다. 어피젤라틴의 아미노산조성과 1단계 및 2단계 어피젤라틴 가수분해물의 아미노산조성 사이에는 거의 차이가 없었고, 감칠맛과 단맛에 관련이 있는 아미노산 함량이 전체의 $68{\sim}72%$에 달한 반면, 쓴맛을 내는 아미노산 함량은 $23{\sim}25%$에 불과하였으며, 1단계 가수분해물에 비해 2단계 가수분해물이 단맛과 감칠맛이 더 좋았다. 1단계 및 2단계 가수분해물의 용해도는 모든 pH영역에서 완전히 용해하였으나, 유화성 및 포말성은 거의 나타나지 않았다. 완충능은 2단계 가수분해물이 가장 높았으나, 점도는 모든 pH영역에서 거의 차이가 없었으며, 어피젤라틴 보다는 가수분해물의 점도가 더 낮았다. 그리고 2단계 가수분해물은 등온흡습도가 가장 높아 수분활성 저하제로서 이용할 수 있을 것으로 판단되었다.

• Journal of Ginseng Research
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• 제24권3호
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• pp.123-127
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• 2000

인삼모상근의 생장에 영향을 미치는 auxin과 CH의 효과를 규명하기 위하여 auxin류인 IBA, MA, NAA와 아미노산 화합물인 CH를 각각 농도별로 처리하여 MS 고체배지와 액체배지에서 50일간 배양후 수거하여 생중량과 건중량을 측정하여 농도별로 생장률을 측정한 결과, 인삼모상근의 생장에 미치는 auxin의 효과는 고체배양시에는 IBA를 1 mg/L의 농도로 암배양하는 것이 가장 효과적이었으며, NAA 1 mg/L의 농도로 광배양 하는 것도 효과적이었다. 반면에 IAA와 CH는 인삼모상근의 생장에 효과가 없었다. 액체배양시에는 IAA와 NAA를 처리했을 때 농도의 증가에 따른 생장의 증가는 보이지 않고 생장의 감소 없이 거의 일정하게 그 수준이 유지되었으며 IBA와 CH는 모두 인삼모상근의 생장을 오히려 억제하는 경향이었다.

• Journal of Microbiology and Biotechnology
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• 제21권1호
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• pp.109-114
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• 2011

In this study, we investigated the feasibility of producing bioethanol from the hydrolysate of rape stem. Specifically, the most ideal yeast strain was screened, and the microaeration was performed by surface aeration on a liquid medium surface. Among the yeast strains examined, Pichia stipitis CBS 7126 displayed the best performance in bioethanol production during the surface-aerated fermentor culture. Pichia stipitis CBS 7126 produced maximally 9.56 g/l of bioethanol from the initial total reducing sugars (about 28 g/l). The bioethanol yield was 0.397 (by the DNS method). Furthermore, this controlled surface aeration method holds promise for use in the bioethanol production from the xylose-containing lignocellulosic hydrolysate of biomass.

• 한국환경과학회지
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• 제16권9호
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• pp.1103-1109
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• 2007

The aim of this study was to isolate mesophilic chicken feather-degrading bacteria and to evaluate feather hydrolysate as alternative biofertilizer. Isolate RS7 was isolated from compost and identified as Bacillus pumilus according to API analysis and l6S rDNA sequencing analysis. Chicken feathers were completely degraded after 5 days of cultivation at $30^{\circ}C$. Feather hydrolysate treated by B. pumilius RS7 positively influenced Helianthus sannuus L. (sunflower) growth (e.g. growth rate, number and dry weight of leave, and flowering rate). These results suggest that feather hydrolysate prepared using B. pumilius RS7 could successfully be used as alternative biofertilizer, thereby reducing the environmental impact of feather waste from the poultry industry.

• KSBB Journal
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• 제10권2호
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• pp.137-142
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• 1995

Sucrose를 탄소원으로 이용할 수 있는 대장균 W 를 A. eutrophus PHA 합성 유전자가 cloning된 높 은 복제수의 plasmid pSYL105로 형질전환하여 단 순배지에서 배양시 $0.21g/\ell$ 의 낮은 PHB 농도를 얻었다. 이에 107}지의 복합질소원을 소량 첨가하여 PHB 합성에 미치는 영향을 살펴 보았다. Tryptone, casamino acids, casein hydrolysate, 혹은 soy bean hydrolysate의 첨가시 PHB 합성 이 10배 이 상 증대되었다. 또한 이때 sucrose로부터의 PHB 수율도 10배 이상 증가하여 이 네 가지 복합질소원 을 척정 농도로 첨가함으로써 sucrose로부터 효과적인 PHB 생산이 가능함을 알았다.

• International Journal of Industrial Entomology and Biomaterials
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• 제21권2호
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• pp.169-174
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• 2010

Hydrolysate silk fibroin (HSF) is a fibrous protein composed of parallel $\beta$-structures and is made from pure silk elements including 18 amino acids, with glycine, alanine, and serine comprising of over 80% of the amino acids. Numerous studies have documented a range of effects of HSF, including moisturizing, antioxidant activity, nervous system disorders, and many more. We investigated whether HSF has anti-obesity effects in vitro. The effects of HSF inhibition on lipid accumulation and acceleration of lipid degradation in 3T3-L1 cells were studied. Treatment of 3T3-L1 cells with HSF caused significant inhibition of cell viability, an increase in glycerol release, and a decreased in adipocyte differentiation. Moreover HSF stimulated downregulated of adipogenic enzyme expressions (PPAR${\gamma}$ and C/EBP${\alpha}$) and up-regulated of fatty oxidation enzyme expressions (CPT-1 and UCP-2). Based on these results, hydrolysate silk fibroin can be suggested as a potential therapeutic substance as part of a prevention or treatment strategy for obesity.

• 한국식품저장유통학회지
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• 제24권4호
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• pp.542-549
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• 2017

In this study, a peptide exhibiting antioxidant activity was isolated from sandfish (Arctoscopus japonicus) roe hydrolysate (SRH) in order to evaluate their practical uses as materials for manufacturing functional foods. The A. japonicus roe protein was hydrolyzed using Collupulin MG, and isolation of antioxidant peptide was performed using ultrafiltration (UF), prep-HPLC, and RP-HPLC. The SRH with a molecular weight below 3 kDa constituted about 38% of the whole hydrolysate, and the fraction with a molecular weight below 3 kDa showed significantly greater antioxidant activity compared to the original SRH and other fractions. The isolation fold of the antioxidant peptide isolated from SRH throughout the four-step procedure was 7.11-fold, and protein yield was 14.8%. The DPPH radical scavenging activity of isolated antioxidant peptide was above 90% at a concentration of 1.0 mg/mL, which was similar to that of the Trolox at a concentration of 0.1 mg/mL. These results suggested that the antioxidant peptide derived from A. japonicus roe could be a useful additive for producing functional foods and protein supplements. However, it is necessary to perform further study the structural characteristics of this antioxidant peptide isolated from A. japonicus roe.

• Preventive Nutrition and Food Science
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• 제20권3호
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• pp.183-189
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• 2015

In the present study, an optimum protease was selected to hydrolyze the egg white liquid protein for the antioxidant peptides. Alcalase treatment yielded the highest amount of ${\alpha}$-amino groups (15.27 mg/mL), while the control (no enzymatic hydrolysis) showed the lowest amount of ${\alpha}$-amino groups (1.53 mg/mL). Alcalase also gave the highest degree of hydrolysis (DH) value (43.2%) and was more efficient for egg white liquid hydrolysis than the other enzymes. The Alcalase hydrolysate had the highest radical-scavenging activity (82.5%) at a concentration of 5.0 mg/mL. The conditions for enzymatic hydrolysis of egg white liquid with Alcalase were selected as substrate : water ratio of 2:1. Five percent Alacalse treatment did not show significant (P>0.05) increases of DH and ${\alpha}$-amino nitrogen content after 24 hhydrolysis. Thirty two hour-hydrolysis with 5% Alcalase is sufficient to make antioxidative egg white liquid hydrolysate from egg white liquid. DPPH and ABTS radical-scavenging activities were significantly (P<0.05) higher after enzymatic digestion. These results suggest that active peptides released from egg-white protein are effective radical-scavengers. Thus, this approach may be useful for the preparation of potent antioxidant products.

• KSBB Journal
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• 제28권2호
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• pp.86-91
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• 2013

To date, various strategies have been studied to increase specific productivity in Chinese hamster ovary (CHO) cell cultures. Also, albumin-fusion platform is being applied to other important bioactive peptides with short half-lives. Here, we investigated the effects of silkworm gland hydrolysate (SGH) on the production of albumin-erythropoietin (Alb-EPO) in transgenic CHO cells. The viable cell density of CHO cells was increased by 13% in the medium containing 1 mg/mL SGH higher than in the control medium without SGH. In addition, the production of Alb-EPO was also 1.26- fold enhanced by reducing the early apoptosis of CHO cells. In conclusion, SGH could be used as a useful supplement for the enhancement of recombinant protein production.

• 현장농수산연구지
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• 제14권1호
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• pp.93-105
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• 2012

서해안 멸치를 이용한 저염 천연조미료를 생산하고자 시판 효소 3종을 사용하여 효소가수분해물을 제조하고 이의 품질특성을 파악하였다. 멸치 효소가수분해물의 주요 유리아미노산은 글루타민산과 라이신이었으며, 전체 유리아미노산의 함량은 글루타민산(12.6%), 리신(8.56%), 발린(7.06%), 아스파탐(5.73%)순으로 나타났다. 휘발성 정미성분은 전체 30개의 성분이 검출되었으며, aldehyde, ketone, cyclic compound 등으로 나타났다. 가수분해물 제조과정 중 히스타민 함량의 변화는 17.1-18.1 mg/100g으로 변패에 대해 안전한 수준이었다.