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http://dx.doi.org/10.7841/ksbbj.2013.28.2.86

Effects of Silkworm Gland Hydrolysate on Albumin-erythropoietin Production in Transgenic Chinese Hamster Ovary Cells  

Choi, Min-Ho (Department of Biological Engineering, Inha University)
Cha, Hyun-Myoung (Department of Biological Engineering, Inha University)
Kim, Sun-Mi (Department of Applied Bioscience, CHA University)
Choi, Yong-Soo (Department of Applied Bioscience, CHA University)
Kim, Dong-Il (Department of Biological Engineering, Inha University)
Publication Information
KSBB Journal / v.28, no.2, 2013 , pp. 86-91 More about this Journal
Abstract
To date, various strategies have been studied to increase specific productivity in Chinese hamster ovary (CHO) cell cultures. Also, albumin-fusion platform is being applied to other important bioactive peptides with short half-lives. Here, we investigated the effects of silkworm gland hydrolysate (SGH) on the production of albumin-erythropoietin (Alb-EPO) in transgenic CHO cells. The viable cell density of CHO cells was increased by 13% in the medium containing 1 mg/mL SGH higher than in the control medium without SGH. In addition, the production of Alb-EPO was also 1.26- fold enhanced by reducing the early apoptosis of CHO cells. In conclusion, SGH could be used as a useful supplement for the enhancement of recombinant protein production.
Keywords
Albumin-EPO; Apoptosis; CHO cell; Silkworm Gland Hydrolysate;
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