• Title/Summary/Keyword: Hybrid Material

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Preparation and Characterization of Silk Fibroin/Gelatin Hybrid Scaffolds (실크 피브로인/젤라틴 하이브리드 지지체의 제조 및 특성분석)

  • Kim, Hye-Lin;Hong, Min-Sung;Kim, Su-Jin;Jo, Han-Su;Yoo, Il-Sou;Lee, Dong-Won;Khang, Gil-Son
    • Polymer(Korea)
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    • v.35 no.5
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    • pp.378-384
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    • 2011
  • Silk fibroin is a biocompatible and slowly biodegradable natural polymer. This natural polymer has excellent mechanical properties, non-toxicity, and non-immunogenic properties and has been demonstrated to support tissue regeneration. Also, gelatin is a natural material derived from collagen by hydrolysis and has an almost identical composition as that of collagen. Silk fibroin/gelatin scaffolds have been fabricated by using the freeze-drying method. To establish the scaffold manufacturing condition for silk fibroin and gelatin, we made scaffolds with various compositions of gelatin, glutaldehyde and silk fibroin. The silk fibroin/gelatin scaffolds were characterized using SEM, DSC, and water absorption ability tests. The cellular proliferation was evaluated by WST assay. These results suggested that a scaffold containing 8% of gelatin, 1% of glutaldehyde and 0.3 g of silk fibroin provided suitable characterstics for cell adhesion and proliferation. In conclusion, the silk fibroin/gelatin scaffold may serve as a potential cell delivery vehicle and a structural basis for tissue engineering.

Functional Genomics for Mass Analysis of Useful Genes in Panax ginseng C.A. Meyer (인삼의 유용유전자원 확보를 위한 기능 유전체연구)

  • Yang, Deok-Chun
    • Proceedings of the Ginseng society Conference
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    • 2004.05a
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    • pp.17-28
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    • 2004
  • As Korean ginseng is hybrid, an individual variation is very severe, and it takes long times in new breeding because it is required 4 years to pick the seed. But, transformation technique makes the high-functional breeding in short time. The focus of these ginseng studies is to find and secure the useful gene. And it is urgent to accumulate the fundamental data for the molecular breeding and secure the useful genes. Therefore, transformation and soil acclimatization technique are necessary to molecular breeding in use of the introduction of functional genes. In this study, it add to secure of new regulation gene and useful gene as to accumulate the fundamental data for the place where it will contribute to raise the national competitive power. To analyze the useful genes in large scale, we constructed CDNA libraries with various tissues, species, and treated tissue. EST analysis of ginseng perform in large scale and build the EST database of ginseng. We perform the full length sequencing about the selected lots of clones that include the entire open reading frame of the amino acid residues and construct cDNA chip with the parental EST clones. Establishment of the transformation and a soil acclimatization system throuth the re-introduction of the selected ginseng gene that related with the secondary metabolism and anti-stress into the ginseng.

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A Study on Thermal Properties of Epoxy Composites with Hybrid Fillers (하이브리드 필러를 함유한 에폭시 복합체의 열적 특성 연구)

  • Lee, Seungmin;Rho, Hokyun;Lee, Sang Hyun
    • Journal of the Microelectronics and Packaging Society
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    • v.26 no.4
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    • pp.33-37
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    • 2019
  • In this study, the graded thermal properties of composites are obtained by difference in specific gravity of fillers including Cu, h-BN and GO powders in epoxy. Relatively heavy powders such as Cu and h-BN compared to GO mostly at the bottom layer, while light GO powders were dispersed in the top layer in the composites. The thermal conductivity of composites was gradually increased from 0.55 (0.52) W/mK to 2.82 (1.37) W/mK for GO/h-BN (GO/Cu) epoxy composites from surface to bottom. On the contrary, the coefficient of thermal expansion was decreased from 51 ppm/℃ to 23 ppm/℃ and from 57 ppm/℃ to 32 ppm/℃ for GO/Cu and GO/h-BN, respectively. The variation of thermal properties in composites is attributed due to intrinsic material properties of filler including thermal conductivity, morphology and the distribution by the specific weight of fillers. This simple strategy for realizing graded thermal composites by introducing different filler materials would be effective heat transfer at interface of heterostructure with large thermal properties such as inorganic semiconductor/plastic, metal/plastic, and semiconductor/metal.

Grouting Injection Effectiveness of a Permeable Compacting Grout using Permeable Compaction Type Packer (침투다짐형 팩커를 이용한 침투다짐 그라우트의 주입 효과)

  • Park, Sung-Yong;Shim, Houng-Gen;Kang, Hee-Jin;Lim, One-Bin;Kim, Yong-Seong
    • Journal of the Korean Geosynthetics Society
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    • v.16 no.2
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    • pp.149-158
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    • 2017
  • Permeating injection is commonly known as an ideal type of injection in grouting reservoir embankment, yet often-combined permeating and fracturing injection grouting operation can disturb the original soil. A grouting method has been regarded as effective and developed to ameliorate the possible disturbance problem. It involves compaction grouting with low expansive pressure near the injection hole and repetitive injection and compaction with grout material that allows ideal permeating injection. This thesis develops Hybrid Grout (ie. HG grout) that allows various application in any ground condition combined together, has high fineness and low viscosity, and expands permeation injection to silty sand. It researches on the injection effect of permeable compaction grout which is done with PC packer and is a combination of HG grout and expansion agent to obtain permeation compaction effect on the area near grout injection spot by developing Permeable Compaction Type Packer(ie. PC packer). As the developed PC packer, HG grout, and and expansion agent (HI-E) are applied to reservoir embankment reinforcement grouting, possibile permeation compaction effect that satisfies reservoir embankment grouting standard is confirmed according to the research.

Visualization of Flow in a Transonic Centrifugal Compressor

  • Hayami Hiroshi
    • 한국가시화정보학회:학술대회논문집
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    • 2002.11a
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    • pp.1-6
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    • 2002
  • How is the flow in a rotating impeller. About 35 years have passed since one experimentalist rotating with the impeller. of a huge centrifugal blower made the flow measurements using a hot-wire anemometer (Fowler 1968). Optical measurement methods have great advantages over the intrusive methods especially for the flow measurement in a rotating impeller. One is the optical flow visualization (FV) technique (Senoo, et al., 1968) and the other is the application of laser velocimetry (LV) (Hah and Krain, 1990). Particle image velocimetries (PIVs) combine major features of both FV and LV, and are very attractive due to the feasibility of simultaneous and multi-points measurements (Hayami and Aramaki, 1999). A high-pressure-ratio transonic centrifugal compressor with a low-solidity cascade diffuser was tested in a closed loop with HFC134a gas at 18,000rpm (Hayami, 2000). Two kinds of measurement techniques by image processing were applied to visualize a flow in the compressor. One is a velocity field measurement at the inducer of the impeller using a PIV and the other is a pressure field measurement on the side wall of the cascade diffuser using a pressure sensitive paint (PSP) measurement technique. The PIV was successfully applied for visualization of an unsteady behavior of a shock wave based on the instantaneous velocity field measurement (Hayami, et al., 2002b) as well as a phase-averaged velocity vector field with a shock wave over one blade pitch (Hayami, et al., 2002a. b). A violent change in pressure was successfully visualized using a PSP measurement during a surge condition even though there are still some problems to be overcome (Hayami, et al., 2002c). Both PIV and PSP results are discussed in comparison with those of laser-2-focus (L2F) velocimetry and those of semiconductor pressure sensors. Experimental fluid dynamics (EFDs) are still growing up more and more both in hardware and in software. On the other hand, computational fluid dynamics (CFDs) are very attractive to understand the details of flow. A secondary flow on the side wall of the cascade diffuser was visualized based either steady or unsteady CFD calculations (Bonaiuti, et al.,2002). EFD and CFD methods will be combined to a hybrid method being complementary to each other. Measurement techniques by image processing as well as CFD calculations give a huge amount of data. Then, data mining technique will become more important to understand the flow mechanism both for EFD and CFD.

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Inflammatory Responses to Hydroxyapatite/Poly(lactic-co-glycolic acid) Scaffolds with Variation of Compositions (하이드록시아파타이트/락타이드 글리콜라이드 공중합체 지지체 조성에 따른 염증 완화 효과)

  • Jang, Ji Eun;Kim, Hye Min;Kim, Hyeongseok;Jeon, Dae Yeon;Park, Chan Hum;Kwon, Soon Yong;Chung, Jin Wha;Khang, Gilson
    • Polymer(Korea)
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    • v.38 no.2
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    • pp.156-163
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    • 2014
  • Hydroxyapatite has osteoconductivity, biocompatibility and noninflammatory, and it has been used clinically as artificial bone. In this study, we prepared hydroxyapatite/poly(lactic-co-glycolic acid) (PLGA) scaffolds using 0, 10, 20, 40 and 60 wt% of hydroxyapatite. We analyzed compressive strength, SEM analysis and FTIR for mechanical property of 3D hydroxyapatite/PLGA scaffolds. For biocompatibility tests, cell proliferation and viability were measured via MTT assay and SEM. We analyzed RT-PCR, FACS, histology (H&E, ED-1) for anti-inflammatory effect. This study showed that hydroxyapatite hybrid scaffolds have low inflammatory reaction compared with the PLGA. This result has a potential for the application of artificial bone graft material.

Breeding of a Mid-Late Maturing Watermelon Cultivar, 'Hanbit' with Resistant to Anthracnose Race 3 (수박 탄저병 Race 3 저항성 중만생종 수박 '한빛' 육성)

  • Huh, Yun-Chan;Hong, Kue-Hyon;Ko, Ho-Cheol;Park, Kyoung-Sub;Park, Dong-Kum;Lee, Joong-Sup;Cho, Myeoung-Cheoul;Lee, Sok-Young;Ko, Kwan-Dal;Lee, Woo-Moon
    • Korean Journal of Breeding Science
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    • v.42 no.6
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    • pp.699-702
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    • 2010
  • Anthracnose is a major limiting factor of the watermelon production in Korea. A mid-late maturing watermelon line, 'Hanbit' with resistance to anthracnose (Colletotrichum orbiculare) race 1 and 3, was developed by the cross between anthracnose resistant cultivar 'AU-Producer' and high quality inbred line '920533' at the National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA). 'Hanbit' produces 5.4 to 9.5 kg of red flesh fruits with clear stripes on skin. Average soluble solid contents are ranged from 10.4 to 11.2$^{\circ}$Bx. The yield and quality of 'Hanbit' was comparable to or better than those harvested from the popular commercial cultivars. 'Hanbit' can be cultivated in open fields and under the protected green house condition. In addition to the commercial production, it can be used as a resistant material in $F_1$ hybrid breeding program.

Evaluation of Surface Condition and Food Solution on the Color Stability of Dental Restoration Materials (표면 상태와 용액에 따른 치과용 수복재료의 색안정도 평가)

  • Woo, Hee-Sun;Kim, Hyo-Jin;Jun, Soo-Kyung
    • The Journal of the Korea Contents Association
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    • v.19 no.4
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    • pp.359-367
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    • 2019
  • For this study, specimens were prepared using five types of composite resin and resin-reinforced glass ionomer Fuji II LC, and 29 days precipitation was done in four kinds of solution, which were soy sauce, gochujang, wine, and distilled water. Ten specimens for each coloring sample were divided into two groups, one those surfaces was polyester film and the other with 1200-grit abrasive paper, and 20 specimens per materials. Color change (${\Delta}{\ast}ab$) in the colored specimens is measured in 2days, 7days, 14days and 29days using spectrophotometer (CM-2600d, Konica, Minolta, Ramsey, NJ). All values were considered significant when P<0.05. 1. Each material is discolored over time under the influence of different storage solutions. 2. In soy source, among the specimens polished with polyester film, color change was observed in resin-modified glass ionomer Fuji II LC, prodigy and compomer F-2000 on day 2. Meanwhile, Fuji II LC showed noticeable color change in specimens prepared with 1200-grit sandpaper on day 2, which was followed by flowable resin on day 7. 3. On day 2, there was a color change with the specimens of flowable resin, resin-modified glass ionomer Fuji II LC and Prodigy among the hybrid specimen in the groups of polyester film in gochujang.

A checklist of Gasan Mt.: an online platform for virtual specimens (온라인 생물정보 플랫폼에 기반한 가산의 관속식물목록)

  • YANG, Sungyu;NAM, Bo-Mi;JANG, JuEun;CHOI, Mi-Jung;CHOI, Goya;CHUNG, Kyong-Sook;CHOI, Hyeok-Jae
    • Korean Journal of Plant Taxonomy
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    • v.50 no.4
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    • pp.453-474
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    • 2020
  • Here, we present an updated checklist based on voucher specimens of vascular plants for Gasan Mt. which is located in Chilgok-gun, Gyeongsangbuk-do, South Korea. The list includes 322 taxa in 212 genera and 81 families of vascular plants, of which 14 are endemic, six are rare plants, 48 are floristic target species, and 14 are considered naturalized in Korea. Based on voucher specimens, Gasan Mt. was the southern limit of Cimicifuga heracleifolia var. bifida Nakai and Iris odaesanensis Y. N. Lee on the Korean peninsula. Moreover, we confirmed a hybrid plant deriving from two Iris parents, I. minutoaurea Makino and I. odaesanensis, in a mountain fortress of Gasan Mt. We also present online databases including 325 voucher specimens deposited at the Korea Institute of Oriental Medicine (KIOM; http://oasis.kiom.re.kr/herblib). This study has great significance as the first floristic study of Korean plants sharing virtual specimens online.

Reduction of VOCs and the Antibacterial Effect of a Visible-Light Responsive Polydopamine (PDA) Layer-TiO2 on Glass Fiber Fabric (Polydopamine (PDA)-TiO2 코팅 유리섬유 직물을 이용한 VOCs의 저감 성능 및 항균성 연구)

  • Park, Seo-Hyun;Choi, Yein;Lee, Hong Joo;Park, Chan-gyu
    • Journal of Environmental Health Sciences
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    • v.47 no.6
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    • pp.540-547
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    • 2021
  • Background: Indoor air pollutants are caused by a number of factors, such as coming in from the outside or being generated by internal activities. Typical indoor air pollutants include nitrogen dioxide and carbon monoxide from household items such as heating appliances and volatile organic compounds from building materials. In addition there is carbon dioxide from human breathing and bacteria from speaking, coughing, and sneezing. Objectives: According to recent research results, most indoor air pollution is known to be greatly affected by internal factors such as burning (biomass for cooking) and various pollutants. These pollutants can have a fatal effect on the human body due to a lack of ventilation facilities. Methods: We fabricated a polydopamine (PDA) layer with Ti substrates as a coating on supported glass fiber fabric to enhance its photo-activity. The PDA layer with TiO2 was covalently attached to glass fiber fabric using the drop-casting method. The roughness and functional groups of the surface of the Ti substrate/PDA coated glass fiber fabric were verified through infrared imaging microscopy and field emission scanning electron microscopy (FE-SEM). The obtained hybrid Ti substrate/PDA coated glass fiber fabric was investigated for photocatalytic activity by the removal of ammonia and an epidermal Staphylococcus aureus reduction test with lamp (250 nm, 405 nm wavelength) at 24℃. Results: Antibacterial properties were found to reduce epidermal staphylococcus aureus in the Ti substrate/PDA coated glass fiber fabric under 405 nm after three hours. In addition, the Ti substrate/PDA coated glass fiber fabric of VOC reduction rate for ammonia was 50% under 405 nm after 30 min. Conclusions: An electron-hole pair due to photoexcitation is generated in the PDA layer and transferred to the conduction band of TiO2. This generates a superoxide radical that degrades ammonia and removes epidermal Staphylococcus aureus.