• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.7
• /
• pp.917-922
• /
• 2008

A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif (ADAMTS1) plays a critical role in follicular rupture and represents a major advance in the proteolytic events that control ovulation. In this study, a 9,026-bp DNA sequence containing the full coding region, all 8 introns and part of the 5'and 3' untranslated region of the porcine ADAMTS1 gene was obtained. Analysis of the ADAMTS1 gene using the porcine radiation hybrid panel indicated that pig ADAMTS1 is closely linkage with microsatellite marker S0215, located on SSC13q49. The open reading frame of its cDNA covered 2,844 bp and encoded 947 amino acids. The coding region of porcine ADAMTS1 as determined by sequence alignments shared 85% and 81% identity with human and mouse cDNAs, respectively. The deduced protein contained 947 amino acids showing 85% sequence similarity both to the human and mouse proteins, respectively. Comparative sequencing of three pig breeds revealed one single nucleotide polymorphism (SNP) within exon 7 of which a G-C substitution at position 6006 changes a codon for arginine into a codon for proline. The substitution was situated within a PvuII recognition site and developed as a PCR-RFLP marker for further use in population variation investigations and association analysis with litter size. Allele frequencies of this SNP were investigated in seven pig breeds/lines. An association analysis in a new Qingping female line suggested that different ADAMTS1 genotypes have significant differences in litter size (p<0.01).

• Journal of Microbiology and Biotechnology
• /
• v.32 no.4
• /
• pp.531-540
• /
• 2022

Due to the high incidence of malignant melanoma, the establishment of in vitro models that recapitulate the tumor microenvironment is of great biological and clinical importance for tumor treatment and drug research. In this study, 3D printing technology was used to prepare GelMA/PEGDA composite scaffolds that mimic the microenvironment of human malignant melanoma cell (A375) growth and construct in vitro melanoma micro-models. The GelMA/PEGDA hybrid scaffold was tested by the mechanical property, cell live/dead assay, cell proliferation assay, cytoskeleton staining and drug loading assay. The growth of tumor cells in two- and three-dimensional culture systems and the anti-cancer effect of luteolin were evaluated using the live/dead staining method and the Cell Counting Kit-8 (CCK-8) method. The results showed a high aggregation of tumor cells on the 3D scaffold, which was suitable for long-term culture. Cytoskeleton staining and immunofluorescent protein staining were used to evaluate the degree of differentiation of tumor cells under 2D and 3D culture systems. The results indicated that 3D bioprinted scaffolds were more suitable for tumor cell expansion and differentiation, and the tumor cells were more aggressive. In addition, luteolin was time- and dose-dependent on tumor cells, and tumor cells in the 3D culture system were more resistant to the drug.

• Journal of Korean Society of Steel Construction
• /
• v.25 no.4
• /
• pp.379-388
• /
• 2013

This paper aims to experimentally investigate the fire resistance of U-shaped hybrid composite beams protected by spay and paint insulations. Subjected to two and three hours of the Standard ISO fire, the flexural performance of 4.4m beams with/without imposed loadings was examined with respect to failure criteria such as deflection and deflection rate of the mid-span and temperatures measured in the steel section. The results demonstrated that the proposed configuration of the composite beam is able to achieve a very competitive 3-hour fire resistance rating in economical aspects.

• The Journal of the Korea institute of electronic communication sciences
• /
• v.13 no.2
• /
• pp.443-448
• /
• 2018

Recently, Hybrid Electric Vehicle(: HEV) is in the spotlight to global warming caused by carbon dioxide and emission reduction. HEV consists of a combination of mechanical engine and electric motor system. The flow of energy required to drive a HEV depends on the driving conditions of the vehicle. In this paper, we study the contents of HEV education using real-time monitoring system. A real-time monitoring system consisting of hardware and virtual programs is used to simulate the overall operation of a HEV through simulations according to driving conditions and to explain how to learn through hardware.

• Structural Engineering and Mechanics
• /
• v.64 no.5
• /
• pp.591-610
• /
• 2017

This paper is concerned with the static analysis of variable thickness of two directional functionally graded porous materials (FGPM) circular plate resting on a gradient hybrid foundation (Horvath-Colasanti type) with friction force and subjected to compound mechanical loads (e.g., transverse, in-plane shear traction and concentrated force at the center of the plate).The governing state equations are derived in terms of displacements based on the 3D theory of elasticity, assuming the elastic coefficients of the plate material except the Poisson's ratio varying continuously throughout the thickness and radial directions according to an exponential function. These equations are solved semi-analytically by employing the state space method (SSM) and one-dimensional differential quadrature (DQ) rule to obtain the displacements and stress components of the FGPM plate. The effect of concentrated force at the center of the plate is approximated with the shear force, uniformly distributed over the inner boundary of a FGPM annular plate. In addition to verification study and convergence analysis, numerical results are displayed to show the effect of material heterogeneity indices, foundation stiffness coefficients, foundation gradient indices, loads ratio, thickness to radius ratio, compressibility, porosity and friction coefficient of the foundation on the static behavior of the plate. Finally, the responses of FG and FG porous material circular plates to compound mechanical loads are compared.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.15 no.3
• /
• pp.312-317
• /
• 2005

Ubiquitous computing has had an effect to politics, economics, society, culture, education and so forth. For effective management of huge Ubiquitous networks environment, various computers which are connected to networks has to decide automatic optimum with intelligence. Currently in many areas, data mining has been used effectively to construct intelligent systems. We proposed a hybrid support vector machine for Ubiquitous data mining which realized intelligent Ubiquitous computing environment. Many data were collected by sensor networks in Ubiquitous computing environment. There are many noises in these data. The aim of proposed method was to eliminate noises from stream data according to sensor networks. In experiment, we verified the performance of our proposed method by simulation data for Ubiquitous sensor networks.

• Journal of Soil and Groundwater Environment
• /
• v.19 no.3
• /
• pp.142-152
• /
• 2014

In this study, generic characteristics of the acid mine drainage (AMD), removal efficiency of iron, aluminium and manganese by chemical treatment, electrolysis and hybrid process using electrolysis after neutralization were evaluated. The pH of AMD was inversely proportional to the rainfall. In dry-season, the average pH of AMD was ranged from 4.5 to 5.5, showing slight variation. However, the pH of AMD was gradually decreased along with rainfall and dropped to 3.02 in September showing the greatest rainfall. Removal efficiency of heavy metals by chemical treatments using three different neutralizing agents or by electrolysis was low. However, a hybrid process performed with electrolysis after addition of neutralization shows higher removal capacity for heavy metal ions than neutralization-alone and electrolysisalone process.

• Polymer(Korea)
• /
• v.31 no.2
• /
• pp.111-116
• /
• 2007

Polyurethane modified acrylate $(PUA)/SiO_2$ hybrid films were prepared by ultraviolet curing and their surface properties were investigated by hardness and adhesion test. The films were examined by the manipulation of mole-ratio of organic to inorganic components. Under the silica content controlled, highly desirable films were achieved and scratch resistance and hardness property of film were also enhanced, which indicates that the crosslinked silica particles are homogeneously dispersed within PUA film.

• Archives of Plastic Surgery
• /
• v.48 no.4
• /
• pp.427-432
• /
• 2021

The conventional approach of looking down a microscope to perform microsurgical procedures is associated with occupational injuries, anti-ergonomic postures, and increased tremor and fatigue, all of which predispose microsurgeons to early retirement. Recently, three-dimensional (3D) visualization of real-time microscope magnification has been developed as an alternative. Despite its commercial availability, no supermicrosurgical procedures have been reported using this technology to date. Lymphovenous anastomoses (LVAs) often require suturing vessels with diameters of 0.2-0.8 mm, thus representing the ultimate microsurgical challenge. After performing the first documented LVA procedure using 3D-augmented visualization in our unit and gaining experience with this technique, we conducted an anonymized in-house survey among microsurgeons who had used this approach. The participants considered that 3D visualization for supermicrosurgery was equivalent in terms of handling, optical detail, depth resolution, and safety to conventional binocular magnification. This survey revealed that team communication, resident education, and ergonomics were superior using 3D digital hybrid visualization. Postoperative muscle fatigue, tremor, and pain were also reduced. The major drawbacks of the 3D visualization microscopic systems are the associated costs, required space, and difficulty of visualizing the lymphatic contrast used.

• Steel and Composite Structures
• /
• v.44 no.3
• /
• pp.407-421
• /
• 2022

Prefabricated hybrid wind turbine towers (WTTs) are promising due to height increase. This study proposes the use of ultra-high performance concrete (UHPC) to develop a new type of WTT without the need to use reinforcement. It is demonstrated that the UHPC WTT structure without reinforcing bars could achieve performance similar to that of reinforced concrete WTTs. To simplify the design of WTT, a design approach for the calculation of stresses at the horizontal joints of a WTT is proposed. The stress distribution near the region of the horizontal joint of the WTT structure under normal operating conditions and different load actions is studied using the proposed approach, which is validated by the finite element method. A further parametric study shows that the degree of prestressing and the bending moment both significantly affect the principal stress. The shear-to-torsion ratio also shows a significant influence on the principal tensile stress.