• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.917-922
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• 2008

A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif (ADAMTS1) plays a critical role in follicular rupture and represents a major advance in the proteolytic events that control ovulation. In this study, a 9,026-bp DNA sequence containing the full coding region, all 8 introns and part of the 5'and 3' untranslated region of the porcine ADAMTS1 gene was obtained. Analysis of the ADAMTS1 gene using the porcine radiation hybrid panel indicated that pig ADAMTS1 is closely linkage with microsatellite marker S0215, located on SSC13q49. The open reading frame of its cDNA covered 2,844 bp and encoded 947 amino acids. The coding region of porcine ADAMTS1 as determined by sequence alignments shared 85% and 81% identity with human and mouse cDNAs, respectively. The deduced protein contained 947 amino acids showing 85% sequence similarity both to the human and mouse proteins, respectively. Comparative sequencing of three pig breeds revealed one single nucleotide polymorphism (SNP) within exon 7 of which a G-C substitution at position 6006 changes a codon for arginine into a codon for proline. The substitution was situated within a PvuII recognition site and developed as a PCR-RFLP marker for further use in population variation investigations and association analysis with litter size. Allele frequencies of this SNP were investigated in seven pig breeds/lines. An association analysis in a new Qingping female line suggested that different ADAMTS1 genotypes have significant differences in litter size (p<0.01).

• Journal of Microbiology and Biotechnology
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• 제32권4호
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• pp.531-540
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• 2022

Due to the high incidence of malignant melanoma, the establishment of in vitro models that recapitulate the tumor microenvironment is of great biological and clinical importance for tumor treatment and drug research. In this study, 3D printing technology was used to prepare GelMA/PEGDA composite scaffolds that mimic the microenvironment of human malignant melanoma cell (A375) growth and construct in vitro melanoma micro-models. The GelMA/PEGDA hybrid scaffold was tested by the mechanical property, cell live/dead assay, cell proliferation assay, cytoskeleton staining and drug loading assay. The growth of tumor cells in two- and three-dimensional culture systems and the anti-cancer effect of luteolin were evaluated using the live/dead staining method and the Cell Counting Kit-8 (CCK-8) method. The results showed a high aggregation of tumor cells on the 3D scaffold, which was suitable for long-term culture. Cytoskeleton staining and immunofluorescent protein staining were used to evaluate the degree of differentiation of tumor cells under 2D and 3D culture systems. The results indicated that 3D bioprinted scaffolds were more suitable for tumor cell expansion and differentiation, and the tumor cells were more aggressive. In addition, luteolin was time- and dose-dependent on tumor cells, and tumor cells in the 3D culture system were more resistant to the drug.

• 한국강구조학회 논문집
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• 제25권4호
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• pp.379-388
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• 2013

본 연구에서는 신형상 U형 하이브리드 합성보의 내화성능평가를 실시하였다. 내화성능평가는 세라믹계의 뿜칠재와 내화페인트로 구분하여 내화시험을 수행하였다. 시험 변수는 피복재의 종류(뿜칠재와 페인트)와 내화시간(2시간, 3시간), 하중가력 유무(재하와 비재하), 뿜칠재 두께 등이다. 내화성능평가 합성보는 기존 철골보로 인정을 받은 제품에 비해 내화성능이 향상되는 것을 확인할 수 있다. 그러나 내화성능 향상에 대한 정량적인 평가는 합성보 형상과 관련한 다양한 변수가 조합되므로 지속적인 검토가 필요할 것으로 판단된다.

• 한국전자통신학회논문지
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• 제13권2호
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• pp.443-448
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• 2018

최근 이산화탄소에 따른 지구온난화 문제와 배기가스의 저감을 위하여 하이브리드 자동차가 각광을 받고 있다. 하이브리드 자동차는 기계적인 엔진과 전기적인 모터시스템이 결합된 동력구조를 갖는다. 차량의 주행조건에 따라 하이브리드 자동차의 구동을 위해 필요로 하는 에너지의 흐름은 달라진다. 본 논문에서는 실시간 모니터링 시스템을 이용한 하이브리드 자동차 교육용 콘텐츠에 관한 연구를 대상으로 한다. 하드웨어와 버추얼 프로그램으로 구성된 실시간 모니터링 시스템을 이용하여 하이브리드 자동차의 전체적인 운행을 주행조건별로 시뮬레이션을 통해 모사하고, 하드웨어를 통해 학습할 수 있는 방법에 대해 기술한다.

• Structural Engineering and Mechanics
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• 제64권5호
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• pp.591-610
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• 2017

This paper is concerned with the static analysis of variable thickness of two directional functionally graded porous materials (FGPM) circular plate resting on a gradient hybrid foundation (Horvath-Colasanti type) with friction force and subjected to compound mechanical loads (e.g., transverse, in-plane shear traction and concentrated force at the center of the plate).The governing state equations are derived in terms of displacements based on the 3D theory of elasticity, assuming the elastic coefficients of the plate material except the Poisson's ratio varying continuously throughout the thickness and radial directions according to an exponential function. These equations are solved semi-analytically by employing the state space method (SSM) and one-dimensional differential quadrature (DQ) rule to obtain the displacements and stress components of the FGPM plate. The effect of concentrated force at the center of the plate is approximated with the shear force, uniformly distributed over the inner boundary of a FGPM annular plate. In addition to verification study and convergence analysis, numerical results are displayed to show the effect of material heterogeneity indices, foundation stiffness coefficients, foundation gradient indices, loads ratio, thickness to radius ratio, compressibility, porosity and friction coefficient of the foundation on the static behavior of the plate. Finally, the responses of FG and FG porous material circular plates to compound mechanical loads are compared.

• 한국지능시스템학회논문지
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• 제15권3호
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• pp.312-317
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• 2005

유비쿼터스 컴퓨팅 환경은 정치, 경제, 사회, 문화, 교육 등 대부분의 분야에 많은 영향을 주고 있다. 인터넷에 비해 훨씬 거대한 유비쿼터스 네트워크 환경이 효과적으로 운영되기 위해서는 네트워크에 접속한 다양한 컴퓨터들이 스스로 지능을 가지고 주어진 상황에서 최적의 의사결정을 할 수 있어야 한다. 현재 많은 분야에서 데이터 마이닝은 지능형 시스템 구축을 위한 효과적인 분석도구로 사용되고 있다. 지능화된 유비쿼터스 컴퓨팅 환경의 구현을 위한 유비쿼터스 데이터 마이닝을 위하여 본 논문에서는 변형된 Support Vector Machine 기법을 제안하였다. 유비쿼터스 컴퓨팅 환경에서 상당 부분의 데이터가 센서를 통하여 수집된다. 센서 네트워크를 통하여 수집된 데이터는 상당부분 잡음을 포함한 데이터이다. 제안 기법은 특히 센서 네트워크를 통한 스트림 데이터의 잡음을 제거하는 데 목적을 두고 있다. 본 논문의 실험에서는 유비쿼터스 센서 네트워크를 나타내는 다양한 분포로부터 시뮬레이션 데이터를 생성하여 제안 방법의 성능 평가를 수행하였다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제19권3호
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• pp.142-152
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• 2014

In this study, generic characteristics of the acid mine drainage (AMD), removal efficiency of iron, aluminium and manganese by chemical treatment, electrolysis and hybrid process using electrolysis after neutralization were evaluated. The pH of AMD was inversely proportional to the rainfall. In dry-season, the average pH of AMD was ranged from 4.5 to 5.5, showing slight variation. However, the pH of AMD was gradually decreased along with rainfall and dropped to 3.02 in September showing the greatest rainfall. Removal efficiency of heavy metals by chemical treatments using three different neutralizing agents or by electrolysis was low. However, a hybrid process performed with electrolysis after addition of neutralization shows higher removal capacity for heavy metal ions than neutralization-alone and electrolysisalone process.

• 폴리머
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• 제31권2호
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• pp.111-116
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• 2007

폴리우레탄 변형 아크릴레이트(PUA)/실리카 하이브리드 필름은 자외선 경화에 의해 제조되었고, 필름의 표면물성은 경도시험과 접착력 테스트를 통해 분석되었다. 이들 필름은 유기, 무기함량을 mole비로 조성하여 실험하였으며, 무기조성은 실리카 입자의 함량을 기준으로 설정하였다. 필름 층 내의 실리카 입자는 함량에 따라 혹은 분산정도에 따라 내스크래치성이나 필름자체 경도 등의 물성이 많은 차이를 보였는데, 이는 PUA필름 층 내의 실리카 입자들이 균일하게 분포되어 가교결합되어 있었기 때문이다.

• Archives of Plastic Surgery
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• 제48권4호
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• pp.427-432
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• 2021

The conventional approach of looking down a microscope to perform microsurgical procedures is associated with occupational injuries, anti-ergonomic postures, and increased tremor and fatigue, all of which predispose microsurgeons to early retirement. Recently, three-dimensional (3D) visualization of real-time microscope magnification has been developed as an alternative. Despite its commercial availability, no supermicrosurgical procedures have been reported using this technology to date. Lymphovenous anastomoses (LVAs) often require suturing vessels with diameters of 0.2-0.8 mm, thus representing the ultimate microsurgical challenge. After performing the first documented LVA procedure using 3D-augmented visualization in our unit and gaining experience with this technique, we conducted an anonymized in-house survey among microsurgeons who had used this approach. The participants considered that 3D visualization for supermicrosurgery was equivalent in terms of handling, optical detail, depth resolution, and safety to conventional binocular magnification. This survey revealed that team communication, resident education, and ergonomics were superior using 3D digital hybrid visualization. Postoperative muscle fatigue, tremor, and pain were also reduced. The major drawbacks of the 3D visualization microscopic systems are the associated costs, required space, and difficulty of visualizing the lymphatic contrast used.

• Steel and Composite Structures
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• 제44권3호
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• pp.407-421
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• 2022

Prefabricated hybrid wind turbine towers (WTTs) are promising due to height increase. This study proposes the use of ultra-high performance concrete (UHPC) to develop a new type of WTT without the need to use reinforcement. It is demonstrated that the UHPC WTT structure without reinforcing bars could achieve performance similar to that of reinforced concrete WTTs. To simplify the design of WTT, a design approach for the calculation of stresses at the horizontal joints of a WTT is proposed. The stress distribution near the region of the horizontal joint of the WTT structure under normal operating conditions and different load actions is studied using the proposed approach, which is validated by the finite element method. A further parametric study shows that the degree of prestressing and the bending moment both significantly affect the principal stress. The shear-to-torsion ratio also shows a significant influence on the principal tensile stress.