• Korean Journal of Pharmacognosy
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• v.32 no.3 s.126
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• pp.175-180
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• 2001

Solvent extracts of 17 plants collected in Cheju Island were investigated for their biological properties related to cosmeceuticals such as tyrosinase and hyaluronidase inhibition and also radical scavenging effects. The chloroform fraction of Phytolacca esculenta root exhibited strong inhibition against tyrosinase activity. No fraction showed significant hyaluronidase inhibition. Some solvent extracts of plants such as Achyranthes japonica and Artemisia princeps showed considerable radical scavenging activities.

• Journal of Applied Biological Chemistry
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• v.65 no.4
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• pp.405-412
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• 2022

Echinodorus cordifolius (L.) is an aquatic plant in the family Alismataceae. The anti-skin aging activity of E. cordifolius (L.) has not been yet reported. Therefore, the objective of the present study was to prepare 70% ethanol extract (ECEE) from E. cordifolius (L.) and investigate their antioxidant and anti-hyaluronidase activities for confirm the potential of anti-skin aging. ECEE showed good activities of DPPH, hydrogen peroxide scavenging, and hyaluronidase inhibition, with EC₅₀ and IC₅₀ values of 31.4, 300, and 450 ㎍/mL, respectively. ECEE also significantly improved cell viability and inhibited intracellular reactive oxygen species dose-dependently against 1 mM hydrogen peroxide-induced oxidative stress in immortalized human keratinocytes (HaCaT cells). Furthermore, ECEE upregulated hyaluronic acid (HA)-synthesizing enzyme hyaluronan synthase 2 (HAS2) expression level, but downregulated expression level of HA-degrading enzyme hyaluronidase 2, resulting in increased HA production in HaCaT cells. Taken together, these results suggest that ECEE shows antioxidant and anti-hyaluronidase potential and could be a functional cosmetic ingredients for anti-skin aging.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.663-665
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• 2003

For the screening of anti-inflammation and antioxidative activities, ethanolic extract of 40 species of traditional herbal medicines were examined in vitro their hyaluronidase inhibitory effect and radical scavenging activity. Astragali Radix, Eucommia Cortex, Schizandrae Fructus, Scutellaria Radix and Moutan Radicis Coritex showed more than 50% hyaluronidase inhibitory effects. Moutan Radicis Cortex, Paeoniae Radix Alba, Plantaginis Semen and Sorbus commixta Hedl. showed more than 90% in electron donating activity. The scavenging effects of ethanolic extracts on hydroxyl radical showed significantly activity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.6
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• pp.844-850
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• 2013

In this study, the optimal extraction conditions for three medicinal herbs as functional sources against inflammatory and arthritic diseases were developed. Traditional medicinal herbs were screened for their inhibition of hyaluronidase (HAse) activity and nitric oxide (NO) synthesis. For the screening of anti-inflammatory properties, ethanolic extracts of 53 species of traditional medicinal herb were examined. We confirmed that Astragalus membranaceus (A.R.), Schisandra chinensis (S.F.), and Platycodon grandiflorum (P.G.) inhibit NO production. For extraction from all three herbs simultaneously, an ethanol concentration of 95%, a 1:2:1 mixture ratio, and at 50 rpm mixing speed, for over 12 h and at $30^{\circ}C$ was the best condition for optimal extract yield and NO inhibition effects. HAse inhibition from the three herb extraction was three fold higher than single samples. The ethanol extracts were fractionated with various solvents (n-hexane, chloroform, ethyl acetate, n-butanol, and water). The ethyl acetate-soluble fraction of the herb mixture showed the highest extract yield (13%) and NO inhibition effects (73%). In conclusion, this study provides experimental evidence that a mixture of P.G., A.R., and S.F. could be used as a source of antioxidant ingredients in the food industry.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.232-232
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• 1994

인삼이 면역계 질환의 일종인 과민성 반응에 대한 치료약으로서의 가능성을 연구하기 위해서 in viva와 in vitra실험을 수행하였다. in vitra실험으로서는 hyaluronidase의 활성을 지표로 삼았으며, in viva실험에서는 mouse이각, rat등피부, 복강 비만세포, 그리고 guinea pig의 ileum에서 면역반응에 대한 아답타겐의 작용을 관찰하였다. in uitra 실험에서는 hyaluronidase와 histamine 분비를 항알레르기 작용의 지표로 사용하였다. hyaluronidase의 경우 그 활성은 Cacl2로 활성화 시키기 전이나 후에 모두 아답타겐(100, 50, 및 10 mg/ml)에 의해서 현저하게 억제되었다. 한편 rat 복강 비만 세포에서의 histamine유리 시험에서는 아답타겐(0.5, 1, 5mg/ml)이 histamine유리가 증가되었다. Guinea pig의 ileum 평활근에서는 아답타겐이 평활근 자극 작용없이 항히스타민 효과률 나타내었다. in uiua 실험의 첫 단계로 우리는 혈관 투과성에 미치는 아답타겐의 효과를 mouse 이각과 rat 등피부에서 관찰하였다. Mouse이각에서의 실험에서는 hoistamine, serotonin, 그리고 LTC4로 challenge하여 관찰한 결과 histamine과 serotonin의 경우, 혈관투과성 항진은 아답타겐(50-400mg/m1)에 의해서 유의성있게 억제되었으나, rat 등 피부에서는 mouse에서와는 달리 48-hr PCA가 증가 되었고, histamine에 의한 혈관투과성 항진은 아답타겐에 의해 영향을 받지 않았다. 반면에 compound 48/80과 serotonin에 의한 혈관투과성 항진은 mouse 의 경우에서처럼 아답타겐에 의해 억제되었다.

• KSBB Journal
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• v.18 no.4
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• pp.282-288
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• 2003

For the screening of anti-inflammation and antioxidative activities, ethanolic extract of 40 species of traditional herbal medicines were examined their hyaluronidase inhibitory effect and radical scavenging activity in vitro. From the result of the hyaluronidase inhibitory activity using a Morgan-Elson assay, Astragali Radix, Eucommia Cortex, Schizandrae Fructus, Scutellaria Radix, Acanthopanacis Cortex, Chaenomelis Fructus, Amomum xanthioides Wallich and Moutan Radicis Cortex showed more than 50% hyaluronidase inhibitory effects at the concentration of 10 mg/mL. In the various solvent fractions (n-hexane, chloroform, ethyl acetate, water) prepared from ethanolic extracts, the ethyl acetate fraction of all extracts tested showed strong activity. Antioxidative activity was evaluated by assaying electron-donating ability to DPPH free radical and scavenging of hydroxyl radical (ㆍOH) generated through Fenton reaction, respectively. Rubus coreanus Miq, Moutan Radicis Cortex, Paeoniae Radix Alba, Plantaginis Semen and Sorbus commixta Hedl. showed high activity more than 90%, yet similar activity to $\alpha$-tocopherol and BHA at the concentration of 1 mg/mL in electron donating activity. The scavenging effects of ethanolic extracts on hydroxyl radical were investigated using a 2-deoxyribose oxidation method and tested all extracts showed significant radical scavenging activity. The experiment was also performed to examine whether herbal medicines having significant lipid peroxidation inhibitory activity, Schizandrae-Fructus is the strongest inhibitory activity in both linoleic acid and liposome peroxidation.

• Korean Journal of Medicinal Crop Science
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• v.12 no.4
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• pp.289-294
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• 2004

Reactive oxygen species (ROS) are continuously produced at a high rate as a by-product of aerobic metabolism. Several lines of evidence provided that ROS appears to cause to develop aging and various diseases. High level of hyaluronic acid with decreased molecular weight has been detected in patients with inflammatory diseases including rheumatoid arthritis. In this study, we have conducted to investigate the antioxidant and hyaluronidase inhibitory activities of Prunus persica Batsch var. davidiana Maximowicz in order to screen the bioactive substances which can be developed as possible anti-inflammatory agents. Among the extracts of Prunus persica Batsch var. davidiana Maximowicz, EtOAc extract exhibited the strongest effect on antioxidant experiment such as DPPH, Ferric- Thiocyanate and Rancimat. Also, EtOAc extract showed a potent hyaluronidase inhibitory activity.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.48 no.3
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• pp.275-285
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• 2022

In this study, we prepared two extracts using 1,3-butylene glycol (DBG) and ethanol (DET), with Diospyros kaki and determined the anti-oxidant, anti-aging, and whitening effects in vitro. Anti-oxidant activity was measured by DPPH and superoxide dismutase (SOD) method, and as a result, both DBG and DET extracts confirmed their anti-oxidant properties by exhibiting significant DPPH scavenging and SOD-like activities. For anti-aging activity, we measured elastase and hyaluronidase inhibition, and the inhibition of MMP-1 expression. Both DBG and DET significantly inhibited elastase and hyaluronidase activities dose-dependently, and MMP-1 expression was also reduced in both extracts. We also measured the whitening effects with tyrosinase activity and melanin production, but only DBG showed a decrease in tyrosinase. In summary, D. kaki extract has strong antioxidant, anti-aging, and whitening functions, and it is believed that it can be used as a cosmetic material in the future.

• Journal of Applied Biological Chemistry
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• v.60 no.3
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• pp.191-198
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• 2017

Hyaluronidase inhibitory activity as inflammatory factor of Koreinsis chinensis leaf ethanol extract was showed higher inhibitory activity than water extract. 29.5% inhibitory activity was shown at concentration of $200{\mu}g/mL$ phenolics. Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells were treated with different concentrations ($5-25{\mu}g/mL$) of Koreinsis chinensis leaf extract and the amount of nitric oxide (NO) was determined; LPS-treated cells produced 3 times more NO than non-LPS treated cells. Moreover, the NO production in cells treated with Koreinsis chinensis leaf extract showed inhibitory effect in a concentration-dependent manner. Due to the stimulant-induced NO production is regulated by the inducible nitric oxide synthase (iNOS), we determined the iNOS protein level to elucidate the mechanism by which the NO production was inhibited. It was reduced by 40% with a Koreinsis chinensis leaf extract concentration of $25{\mu}g/mL$ and identified iNOS inhibition in dose-dependent manner. The prostaglandin $E_2$ production in cells treated with Koreinsis chinensis leaf extract was reduced by 26.2% at concentration of $25{\mu}g/mL$. The protein expression of cyclooxygenase-2 in LPS-treated Raw 264.7 cells was inhibited by 64% at $25{\mu}g/mL$ of Koreinsis chinensis leaf extract. Koreinsis chinensis leaf extract had a concentration-dependent inhibitory effect on the production of tumor necrosis factor-${\alpha}$ and interleukin-6 as pro-inflammatory cytokine in LPS-treated Raw 264.7 cells at $25{\mu}g/mL$ of Koreinsis chinensis leaf extract. Their levels were decreased by 61.7 and 62% respectively.

• Journal of Applied Biological Chemistry
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• v.63 no.3
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• pp.221-227
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• 2020

This study was designed to assess the change of the antioxidative and biological enzyme activities [tyrosinase, elastase, collagenase, and hyaluronidase (HAase)] of extracts from elicitor-treated Oplismenus undulatifolius. Elicitor-treated O. undulatifolius showed higher total phenolic content than the non-treated extract. As a result of comparing the anti-oxidant activity of elicitor-treated O. undulatifolius extract and non-treated extract, the elicitor-treated group showed high activity. Elicitor-treated O. undulatifolius showed higher elastase and collagenase inhibitory activities as anti-wrinkle effect, tyrosinase inhibitory activity as whitening effect, and anti-inflammation effect to induced as HAase inhibition than the non-treated extract. Therefore, elicitor-treatment during O. undulatifolius cultivation in outdoors will elevate total phenolics content in the plant and elevate of various bioactivities, which will yield high quality for industrialization.