• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.2
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• pp.147-153
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• 2021

Collagen hydrolysate (CH) is known to prevent skin aging by stimulating skin dermal fibroblasts to promote synthesis of extracellular matrix such as collagen and elastin. Recently, among the various factors that cause skin aging, advanced glycation end products (AGEs) have received particular attention. However, the effect of CH on AGE accumulation has not been studied. Since CH could affect AGE accumulation by promoting production of skin structural proteins, clinical trial was performed using low molecule collagen peptide (LMCP), which were CH containing 25% tripeptide and 4% Gly-Pro-Hyp. Skin autofluorescence (SAF) values were measured using an AGE reader to evaluate accumulation of AGE in skin. As a result of applying 0.5% and 1.0% LMCP solutions to the subject's forearm for 8 weeks, the SAF value at the test site significantly decreased compared to the control site. Additionally, in vitro test was performed using CCD-986sk to evaluate the promotion of collagen synthesis in skin fibroblasts by LMCP. As a result, 800 ㎍/mL of LMCP significantly increased synthesis of human pro-collagen Iα1 (COL1A1) in CCD-986sk. Through this study, we have confirmed that tropical LMCP applications can promote collagen synthesis to help anti-glycation effects, suggesting that LMCP has potential as an anti-aging cosmetic material.

• YAKHAK HOEJI
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• v.56 no.1
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• pp.9-13
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• 2012

We previously reported that the extract of Taraxacum platycarpum (AF-343) had several biological properties such as skin hydration and anti-inflammatory effects, thereby AF-343 be a promising anti-atopic dermatitis agent. However, few studies have been conducted to evaluate its effect on modulation of extracellular matrix proteins in human skin fibroblasts. The purpose of this study was to investigate the expressions of type I collagen, MMP-1, Smad2/3, and TIMP-1 proteins in AF-343-treated human skin fibroblasts. Human skin fibroblasts were treated by various concentrations of AF-343 (0~2 mg/ml). The expressions of type I collagen, matrix metalloproteinase-1 (MMP-1), Smad2/3, and TIMP-1 proteins were analyzed by Western blot analysis. In addition, level of type I collagen mRNA was analyzed by CAT assay. Expression of type I collagen protein was increased in AF-343-treated human skin fibroblasts by dose and time-dependent manners. Consistent with this result, the expressions of phospho-Smad2/3 in skin fibroblasts were increased and MMP-1 expression was decreased by AF-343 treatment. TIMP-1 expression was not significantly changed in AF-343 treated skin fibroblasts. Extract of Taraxacum platycarpum (AF-343)-induced up-regulation of type I collagen expression was through increased expression of phospho-Smad2/3. These results were occurred combined with down-regulation of MMP-1 in skin fibroblasts. Taken together, this study indicated that AF-343 has property of the modulation of ECM in tissue as well as skin hydration and anti-inflammation.

• The Journal of Advanced Prosthodontics
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• v.10 no.6
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• pp.422-429
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• 2018

PURPOSE. Accurate color matching of maxillofacial prostheses to skin is important for esthetics. A computerized color matching system specific to human skin has recently been developed. The purpose of this study was to evaluate the accuracy in color and translucency matching of the computerized color matching system across different skin colors. MATERIALS AND METHODS. The silicone was colored to simulate 28 different skin colors (n=5) to serve as "target skin colors". Using a spectrocolorometer (e-skin), color codes were determined for "replicate skin color" fabrication. CIELAB Delta-E between target skin color-replicate skin color pairs and translucency parameter were calculated. CIELAB Delta-E values were compared with one-way ANOVA and Tukey multiple-comparison. The agreement between $L^*$, $a^*$, $b^*$ and translucency parameter of target skin colors and replicate skin color were calculated by a two-way mixed average measures intraclass correlation coefficient. Translucency parameter of target skin color- replicate skin color pairs were compared with Paired t-test (${\alpha}=.05$). RESULTS. The mean CIELAB Delta-E value was 3.83 and significant differences were found among colors. The intraclass correlation coefficient showed excellent reliability for $L^*$, $a^*$, $b^*$ and good reliability for translucency parameter (P<.001). The mean translucency parameter of replicate skin colors was significantly higher than that of translucency parameter. CONCLUSION. The computerized color matching system specific to human skin was found to be reliable in terms of color and translucency between target skin colors and replicate skin color.

• Environmental Analysis Health and Toxicology
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• v.29
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• pp.4.1-4.10
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• 2014

Objectives Effects of nanoparticles including zinc oxide nanoparticles, titanium oxide nanoparticles, and their mixtures on skin corrosion and irritation were investigated by using in vitro 3D human skin models ($KeraSkin^{TM}$) and the results were compared to those of an in vivo animal test. Methods Skin models were incubated with nanoparticles for a definite time period and cell viability was measured by the 3-(4, 5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide method. Skin corrosion and irritation were identified by the decreased viability based on the pre-determined threshold. Results Cell viability after exposure to nanomaterial was not decreased to the pre-determined threshold level, which was 15% after 60 minutes exposure in corrosion test and 50% after 45 minutes exposure in the irritation test. IL-$1{\alpha}$ release and histopathological findings support the results of cell viability test. In vivo test using rabbits also showed non-corrosive and non-irritant results. Conclusions The findings provide the evidence that zinc oxide nanoparticles, titanium oxide nanoparticles and their mixture are 'non corrosive' and 'non-irritant' to the human skin by a globally harmonized classification system. In vivo test using animals can be replaced by an alternative in vitro test.

• Toxicological Research
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• v.32 no.4
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• pp.311-316
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• 2016

Effects of nanoparticles (NPs) on skin corrosion and irritation using three-dimensional human skin models were investigated based on the test guidelines of Organization for Economic Co-operation and Development (OECD TG431 and TG439). EpiDerm$^{TM}$ skin was incubated with NPs including those harboring iron (FeNPs), aluminum oxide (AlNPs), titanium oxide (TNPs), and silver (AgNPs) for a defined time according to the test guidelines. Cell viabilities of EpiDerm$^{TM}$ skins were measured by the 3-(4, 5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide based method. FeNPs, AlNPs, TNPs, and AgNPs were non-corrosive because the viability was more than 50% after 3 min exposure and more than 15% after 60 min exposure, which are the non-corrosive criteria. All NPs were also non-irritants, based on viability exceeding 50% after 60 min exposure and 42 hr post-incubation. Release of interleukin 1-alpha and histopathological analysis supported the cell viability results. These findings suggest that FeNPs, AlNPs, TNPs, and AgNPs are 'non-corrosive' and 'non-irritant' to human skin by a globally harmonized classification system.

• Journal of Microbiology and Biotechnology
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• v.32 no.2
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• pp.238-247
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• 2022

Since the skin covers most surfaces of the body, it is susceptible to damage, which can be fatal depending on the degree of injury to the skin because it defends against external attack and protects internal structures. Various types of artificial skin are being studied for transplantation to repair damaged skin, and recently, the production of replaceable skin using three-dimensional (3D) bioprinting technology has also been investigated. In this study, skin tissue was produced using a 3D bioprinter with human skin cell lines and cells extracted from mouse skin, and the printing conditions were optimized. Gelatin was used as a bioink, and fibrinogen and alginate were used for tissue hardening after printing. Printed skin tissue maintained a survival rate of 90% or more when cultured for 14 days. Culture conditions were established using 8 mM calcium chloride treatment and the skin tissue was exposed to air to optimize epidermal cell differentiation. The skin tissue was cultured for 14 days after differentiation induction by this optimized culture method, and immunofluorescent staining was performed using epidermal cell differentiation markers to investigate whether the epidermal cells had differentiated. After differentiation, loricrin, which is normally found in terminally differentiated epidermal cells, was observed in the cells at the tip of the epidermal layer, and cytokeratin 14 was expressed in the lower cells of the epidermis layer. Collectively, this study may provide optimized conditions for bioprinting and keratinization for three-dimensional skin production.

• Proceedings of the Korea Society of Costume Conference
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• 2003.10a
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• pp.39-39
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• 2003

The currently used personal color analysis is mostly based on Westerners' skin colors. As a result, the suggested colors are often not suitable for Korean people's skin colors. Accordingly, the purpose of this study is to develop 'Personal Color Design System(PCDS)' that can suggest fashionable colors suitable for Korean skin colors and personal color types. For this, the system was verified by customers testing and questionnaires, while the system modification and complementary measures were conducted.

• Proceedings of the IEEK Conference
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• 1999.11a
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• pp.531-535
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• 1999

Automatic human face detection in a complex background is one of the difficult problems. In this paper, we propose an effective and robust automatic face detection approach that can locate the face region in natural scene images when the system is used as a pre-processor of a face recognition system . We use two natural and powerful visual cues, the skin color and the eyes. In the first step of the proposed system, the method based on the human skin color space by selecting flesh tone regions using normalized r-g space in color images. In the next step, we extract eye features by calculating moments and using geometrical face model. Experimental results demonstrate that the approach can efficiently detect human faces and satisfactory deal with the problems caused by bad lighting condition, skew face orientation.

• Journal of Computational Design and Engineering
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• v.1 no.1
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• pp.1-12
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• 2014

In this paper, we propose a new method of reconstructing the hand models for individuals, which include the link structure models, the homologous skin surface models and the homologous tetrahedral mesh models in a reference posture. As for the link structure model, the local coordinate system related to each link consists of the joint rotation center and the axes of joint rotation, which can be estimated based on the trajectories of optimal markers on the relative skin surface region of the subject obtained from the motion capture system. The skin surface model is defined as a three-dimensional triangular mesh, obtained by deforming a template mesh so as to fit the landmark vertices to the relative marker positions obtained motion capture system. In this process, anatomical dimensions for the subject, manually measured by a caliper, are also used as the deformation constraints.

• Journal of the Korean Society of Industry Convergence
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• v.1 no.2
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• pp.65-74
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• 1998

The purpose of this paper is to investigate the mount of relief of human solar heat load and thermal comfort in outdoor environment in summer, Six different types of sites, T garden and its neighboring area in Japan, were selected as the experiment sites. The experiments were conducted from 22 to 29 August, 1994 to find the relationship between climatic conditions and human responses, Climatic conditions, subjects's thermal sensation and skin temperature were measured. Radiant heat exchange on the human body was estimated on the basis of the measured air and surface temperature and solar radiation. Thermal index Operative Temperature and New Effective Temperature was modified with the effect of the radiant heat exchange. Human thermal comfort and skin temperature is affected by the solar radiation and the sky factor in an outdoor environment. The effect of tree shade was verified on thermal comfort, The mount of relief of human solar heat load is relation to the existence of shade a solar radiation and the sky factor. The urban garden is one of the effective design element in an urban environmental planning.