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http://dx.doi.org/10.5487/TR.2016.32.4.311

Skin Corrosion and Irritation Test of Nanoparticles Using Reconstructed Three-Dimensional Human Skin Model, EpiDermTM  

Kim, Hyejin (College of Pharmacy, Dongduk Women's University)
Choi, Jonghye (College of Pharmacy, Dongduk Women's University)
Lee, Handule (College of Pharmacy, Dongduk Women's University)
Park, Juyoung (College of Pharmacy, Dongduk Women's University)
Yoon, Byung-Il (College of Veterinary Medicine, Kangwon National University)
Jin, Seon Mi (College of Medicine, Eulji University)
Park, Kwangsik (College of Pharmacy, Dongduk Women's University)
Publication Information
Toxicological Research / v.32, no.4, 2016 , pp. 311-316 More about this Journal
Abstract
Effects of nanoparticles (NPs) on skin corrosion and irritation using three-dimensional human skin models were investigated based on the test guidelines of Organization for Economic Co-operation and Development (OECD TG431 and TG439). EpiDerm$^{TM}$ skin was incubated with NPs including those harboring iron (FeNPs), aluminum oxide (AlNPs), titanium oxide (TNPs), and silver (AgNPs) for a defined time according to the test guidelines. Cell viabilities of EpiDerm$^{TM}$ skins were measured by the 3-(4, 5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide based method. FeNPs, AlNPs, TNPs, and AgNPs were non-corrosive because the viability was more than 50% after 3 min exposure and more than 15% after 60 min exposure, which are the non-corrosive criteria. All NPs were also non-irritants, based on viability exceeding 50% after 60 min exposure and 42 hr post-incubation. Release of interleukin 1-alpha and histopathological analysis supported the cell viability results. These findings suggest that FeNPs, AlNPs, TNPs, and AgNPs are 'non-corrosive' and 'non-irritant' to human skin by a globally harmonized classification system.
Keywords
Nanoparticles; Skin model; Alternative methods; Skin corrosion; Skin irritation;
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