• Journal of Microbiology and Biotechnology
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• 제12권3호
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• pp.483-489
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• 2002

The role of $G{\alpha}12\;and\;G{\alpha}13$ in modulating the IgE receptor-mediated histamine secretion in the streptolysin-o-permeabilized human cultured mast cell was investigated. The expression of $G{\alpha}12\;and\;G{\alpha}13$ proteins were regulated during human cultured mast cell differentiation, and a significant correlation was observed between the levels of expression of $G{\alpha}12\;and\;G{\alpha}13$ proteins and IgE receptor-mediated histamine secretion capability in human cultured mast cells. Antibodies against $G{\alpha}12\;and\;G{\alpha}13$ effectively inhibited the IgE receptor-induced histamine release, and the concentration of anti-$G{\alpha}12$ antibody used to inhibit histamine secretion was shown to also inhibit the IgE receptor-mediated elevation of intracellular $Ca^2+$. Therefore, the results suggest that $G{\alpha}12\;and\;G{\alpha}13$ play roles in modulating IgE receptor-activated $Ca^2+$ influx, thereby regulating histamine release in cultured human mast cells. This is the first report to show that $G{\alpha}12\;and\;G{\alpha}13$ are involved in the regulation of $Ca^2+$ mediated exocytosis in human cultured mast cells.

• 대한약리학회:학술대회논문집
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• 대한약리학회 2006년도 58th Annual Meeting of The Korean Society of Pharmacology
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• pp.68.1-68.1
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• 2006

• 동아시아식생활학회지
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• 제17권1호
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• pp.43-50
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• 2007

This study investigated the effects of mycelia of Lentinus edodes mushroom-cultured Glycyrrihiza radix(LMG) on cancer cell lines and sarcoma 180(S-180), as well as on human mast cells. In an anti-cancer tests using Hep3B(hepatic cancer cell), MCF-7(breast cancer), and HeLa(uterine cancer) cells, LMG extract exhibited greater anti-proliferation effects than Glycyrrihiza glabra(GG) extract. LMG extract multiplication restraining effects were 60% that of ethanol at 3 mg/mL extract also displayed tumor suppressive effects in mice injected with S-180 cells. The growth-inhibition rates against tumor cells were 56% for LMG and 37% for GG. When LMG was added to human mast cells, the Intensity of RT-PCR products using primers($FC{\varepsilon}RI\;c-kit$) decreased. significantly compared with that of control. These results suggest that Lentinus edodes Mushroom-Cultured Glycyrrhiza glabra has an anti-proliferation effects against cancer cell lines(Hep3B, MCF-7 and HeLa) and S-180 tumors and will be also beneficial in treating allergic reactions.

• International Journal of Stem Cells
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• 제15권3호
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• pp.311-323
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• 2022

Background and Objectives: Human mesenchymal stem cells (MSCs) are emerging as a treatment for atopic dermatitis (AD), a chronic inflammatory skin disorder that affects a large number of people across the world. Treatment of AD using human umbilical cord blood-derived MSCs (hUCB-MSCs) has recently been studied. However, the mechanism underlying their effect needs to be studied continuously. Thus, the objective of this study was to investigate the immunomodulatory effect of epidermal growth factor (EGF) secreted by hUCB-MSCs on AD. Methods and Results: To explore the mechanism involved in the therapeutic effect of MSCs for AD, a secretome array was performed using culture medium of hUCB-MSCs. Among the list of genes common for epithelium development and skin diseases, we focused on the function of EGF. To elucidate the effect of EGF secreted by hUCB-MSCs, EGF was downregulated in hUCB-MSCs using EGF-targeting small interfering RNA. These cells were then co-cultured with keratinocytes, Th2 cells, and mast cells. Depletion of EGF disrupted immunomodulatory effects of hUCB-MSCs on these AD-related inflammatory cells. In a Dermatophagoides farinae-induced AD mouse model, subcutaneous injection of hUCB-MSCs ameliorated gross scoring, histopathologic damage, and mast cell infiltration. It also significantly reduced levels of inflammatory cytokines including interleukin (IL)-4, tumor necrosis factor (TNF)-α, thymus and activation-regulated chemokine (TARC), and IL-22, as well as IgE levels. These therapeutic effects were significantly attenuated at all evaluation points in mice injected with EGF-depleted hUCB-MSCs. Conclusions: EGF secreted by hUCB-MSCs can improve AD by regulating inflammatory responses of keratinocytes, Th2 cells, and mast cells.

• Advances in Traditional Medicine
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• 제6권1호
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• pp.39-44
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• 2006

Ji-Jang-Soo (JJS) is known to have a detoxification effect. However, it is still unclear how JJS has these effects in experimental models. In this study, we investigated the effect of JJS on the viability of cells and production of cytokines in human T-cell line, MOLT-4 cells, and human mast cell line, HMC-1 cells. The MOLT-4 cells were cultured for 24 h in the presence or absence of JJS. As the result, JJS (1/100 dilution) significantly increased the cell viability about 78% (P < 0.05) and also increased the interleukin (IL)-2, and interferon $(IFN)-{\gamma}$ production compared with media control at 24 h. But had no effect on IL-4 production. Hypoxia mimic compound, desferroxamine (DFX) decreased the immune cell viability. Cell viability decreased by DFX was increased by JJS. In conclusion, these data indicate that JJS may have an immune-enhancing effect.

• IMMUNE NETWORK
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• 제9권2호
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• pp.41-45
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• 2009

Activating and inhibitory cell surface receptors play important roles in regulation of immune responses. Recent progress has demonstrated that many inhibitory receptors pair with activating, as well as inhibitory, isoforms, both of whose genes are located in small clusters on a chromosome. We and others identified paired activating and inhibitory immunoglobulin-like receptors, designated myeloid-associated immunoglobulin-like receptors (MAIR) (CD300). MAIR is a multigene family consisting of nine genes on a small segment of mouse chromosome 11. MAIR family receptors are preferentially expressed on myeloid cells, including macrophages, dendritic cells, granulocytes, and bone-marrow-derived cultured mast cells, and a subset of B cells and regulate activation of these cells. Thus, MAIR plays an important role in innate immunity mediated by myeloid cells.

• Journal of Microbiology and Biotechnology
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• 제19권2호
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• pp.155-160
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• 2009

Mast cells and basophils perform important functions as pivotal effector cells in IgE-mediated allergic reactions. KU812F cells, a human basophilic cell line isolated originally from chronic myelocytic leukemia, express a high affinity receptor of IgE, $Fc{\varepsilon}RI$. Kaempferol was extracted and isolated from a methanolic extract of flavonoid-rich Nelumbo nucifera stamens. In the present study, the inhibitory effects of kaempferol on $Fc{\varepsilon}RI$ expression in human basophilic KU812F cells was examined. Flow cytometric analysis revealed that $Fc{\varepsilon}RI$ expression on the cell surface was suppressed in a concentration-dependent manner when the cells were cultured with kaempferol. Moreover, RT-PCR analysis showed that the mRNA levels for $Fc{\varepsilon}RI$ ${\alpha}-\;and\;{\gamma}$-chains were reduced as the result of kaempferol treatment in a concentration-dependent manner. Kaempferol showed its suppressive effects on intracellular $Ca^{2+}$ concentration and histamine release from anti-$Fc{\varepsilon}RI$ ${\alpha}$-chain antibody-stimulated cells in a concentration-dependent manner. These observations indicate that kaempferol may exert antiallergic effect via down regulation of $Fc{\varepsilon}RI$ expression and degranulation.

• 생명과학회지
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• 제23권2호
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• pp.213-221
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• 2013

본 연구에서는 우리나라 전통 음식인 비빔밥의 우수성을 검증하고자 비빔밥에 들어가는 재료인 애호박, 도라지, 숙주, 속대기, 부추, 고사리의 조리 전과 후의 항염증 및 항산화 효과를 조사하였다. 인간유래 비만세포인 HMC-1 (human mast cell)에 70% ethanol로 추출한 조리 전, 후의 다양한 비빔밥 재료의 추출물을 1.0 mg/ml의 농도로 처리한 후, PMA와 A23187로 자극하여 염증반응을 유도하였다. MTT 분석에 의한 세포 생존율 결과, 모든 구에서 82% 이상의 세포 생존율을 보여 조리 전, 후의 비빔밥 재료 추출물에 의한 세포 독성은 나타나지 않았다. HMC-1 비빔밥 재료의 추출물을 0.01, 0.1, 1.0 mg/ml의 농도로 처리한 후, 자극제로 염증반응을 유도하였을 때 그 효과를 TNF-${\alpha}$, IL-6, IL-8의 분비량으로 알아보았다. 전체적으로 cytokine의 분비가 감소하였는데, 특히 1.0 mg/ml 농도에서 조리 후의 부추 추출물이 TNF-${\alpha}$, IL-6 분비량을 각각 90%, 93% 저해하였고, 무 추출물은 IL-8 분비량을 85% 저해하여 다양한 비빔밥 재료 중 가장 높은 저해율을 나타냈다. DPPH 분석에 의한 항산화 활성결과, 조리 전, 후의 부추 추출물에서 각각 67.50%, 73.65%로 재료 중 가장 높았으며 전반적으로, 조리함으로써 항산화 활성이 향상되었다. 결론적으로 조리 전보다 조리 후의 재료에서 세 가지의 cytokine을 유의성 있게 억제하였고, 항산화 활성 또한 높게 나타나 비빔밥의 우수성을 검증하는 기초자료로 활용이 가능하다고 사료된다.

• 생명과학회지
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• 제19권1호
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• pp.101-110
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• 2009

유전자 발현의 조절은 세포주기 조절에 중요한 역할을 한다. 본 연구에서 새로운 $mas2^+$ (${\underline{m}}itosis$ ${\underline{as}}sociated$ protein) 유전자는 인간의 SMARCAD1와 상동성을 갖고 분열형 효모인 Schizosaccharomyces pombe (S. pombe)에서 gene-specific PCR 방법에 의해서 분리하였다. 분리된 유전자는 SNF2 도메인이 위치해 있고, 이것은 염색체 재구성에 관련되어 있다. $adh1^+$을 이용한 $mas2^+$ 전사체의 발현량 분석은 $mas2^+$의 발현수준은 S. pombe에서 격막 형성 전에 가장 높았다. $mas2^+$ 완전돌연변이의 세포분열은 26와 $35^{\circ}C$에서 지연되는 현상이 보였고, 다수익 다중 격막이나 핵분열이 일어나지 않는 세포들을 발견하였다. 세포들을 완전배지인 YES에서 증식을 증가시키기 위해서 배양했을 때, 정상과 다른 형태의 표현형을 가진 $mas2^+$ 완전돌연변이 세포들이 증가했다. 이런 표현형들은 $mas2^+$ 유전자의 과발현에 의해서 감소하였다. Mas2 단백질은 S. pombe의 핵내에 위치하였다. 이런 결과들은 $mas2^+$가 인간의 SMARCAD1과 상동성을 갖고 있고, 염색체 재구성과 격막 형성 조절에 사용된다는 것을 나타낸다.