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http://dx.doi.org/10.5352/JLS.2009.19.1.101

Intracullular Functions of the mas2+ Gene in the Fission Yeast, Schizosaccharomyces pombe  

Sin, Sang-Min (Faculty of Biological Science and Research Institute for Basic Science, Wonkwang University)
Cha, Jae-Young (Faculty of Biological Science and Research Institute for Basic Science, Wonkwang University)
Ha, Se-Eun (Faculty of Biological Science and Research Institute for Basic Science, Wonkwang University)
Sim, Sun-Mi (Faculty of Biological Science and Research Institute for Basic Science, Wonkwang University)
Kim, Hyoung-Do (Faculty of Biological Science and Research Institute for Basic Science, Wonkwang University)
Lee, Jung-Sup (Department of Biological Science Research Center for Proteineous Materials, Chosun University)
Park, Jong-Kun (Faculty of Biological Science and Research Institute for Basic Science, Wonkwang University)
Publication Information
Journal of Life Science / v.19, no.1, 2009 , pp. 101-110 More about this Journal
Abstract
The regulation of gene expression plays an important role in cell cycle controls. In this study, a novel $mas2^+$ (mitosis associated protein) gene, a homolog of human SMARCAD1 was isolated and characterized from a fission yeast Schizosaccharomyces pombe (S. pombe) using gene-specific polymerase chain reaction. The isolated gene contained a complete open reading frame capable of encoding 922 amino acid residues with a typical promoter, as judged by nucleotide sequence analysis. It was also found that an SNF2 domain is located, which is involved in the chromosome remodeling. The quantitative analysis of the $mas2^+$ transcript against $adh1^+$ showed that the expression level of $mas2^+$ is high before septum formation in S. pombe. When $mas2^+$ null mutant cells were grown at 27 and $35^{\circ}C$, the cytokinesis of $mas2^+$ null mutant was greatly delayed and a large number of multi-septate and mis-segregated cells were produced. In addition, the number of multi-septate cells significantly increased. When cells were cultured in YES rich medium to increase proliferation, the abnormal phenotypes $mas2^+$ null mutant dramatically increased. These phenotypes could be rescued by an over-expression of the mast gene. The Mas2 protein localized in the nuclei of S. pombe, as evidenced by Mas2-EGFP signals. These results suggest that the $mas2^+$ is homologous to human SMARCAD1 gene and involved in septum formation and chromosome remodeling control.
Keywords
Schizosaccharomyces pombe; Mas2-EGFP; $mas2^+$; SMARCAD1;
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