• 대한한의학방제학회지
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• 제30권2호
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• pp.59-66
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• 2022

Objectives : This study is to investigate whether Carthami flos exhibits a synergistic effect on the apoptotic effect of doxorubicin on human gastric cancer cells. Methods : We used AGS, a human gastric cancer cell line. To investigate the apoptotic efficacy of doxorubicin and Carthami flos, MTT and CCK-8 methods were used. To confirm apoptosis, cell cycle and mitochondrial membrane potential changes were confirmed. To investigate the mechanism of apoptosis, the reactive oxygen species (ROS) experiment was performed. Results : 1. Doxorubicin or Carthami flos induced cell death in the human gastric cancer cell line AGS. 2. Carthami flos showed a synergistic effect of cell death by doxorubicin. 3. The cell cycle and mitochondrial membrane potential changes revealed that cell death was apoptosis. 4. Apoptosis was related to reactive oxygen species (ROS) generation. Conclusions : This result shows the anticancer synergistic effect of Carthami flos in gastric cancer cells, and is considered to be an important basis for the development of anticancer drugs for Carthami flos.

• 유통과학연구
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• 제18권9호
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• pp.13-18
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• 2020

Purpose: Research and development of human capital in countries bring sustainable development to the nations. Especially for developing countries, the attraction of foreign direct investment not only brings economic growth to the country but also contributes to improving human capital. This study aims to assess the impact of foreign direct investment on human capital in ASEAN countries. Research design, data and methodology: With data collected from ASEAN countries from 1990 to 2019, panel data analysis is performed with revised model types (the Pooled OLS, Fixed effect model, Random effect model and regression with Driscoll-Kraay standard errors). Result: The results of the regression analysis show that FDI has a positive impact on human capital. At the same time, the study also found that public investment in education also positively affects human capital; the life expectancy factor does not affect human capital. Conclusions: With this research result, the authors also proposed a number of solutions to improve human capital by attracting FDI and improving the efficiency of investment for the education of ASEAN countries. Besides, public expenditure on education also plays an important role in raising human capital. Therefore, investment in education should be promoted further in the future.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2011년도 제40회 동계학술대회 초록집
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• pp.454-454
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• 2011

Graphene shows unusual electronic properties, such as carrier mobility as high as 10,000 $cm^2$/Vs at room temperature and quantum electronic transport, due to its electronic structure. Carrier mobility of graphene is ten times higher than that of Silicon device. On the one hand, quantum mechanical studies have continued on graphene. One of them is quantum Hall effect which is observed in graphene when high magnetic field is applied under low temperature. This is why two dimension electron gases can be formed on Graphene surface. Moreover, quantum Hall effect can be observed in room temperature under high magnetic field and shows fractional quantization values. Quantum Hall effect is important because quantized Hall resistances always have fundamental value of h/$e^2$ ~ 25,812 Ohm and it can confirm the quantum mechanical behaviors. The value of the quantized Hall resistance is extremely stable and reproducible. Therefore, it can be used for SI unit. We study to measure quantum Hall effect in CVD graphene. Graphene devices are made by using conventional E-beam lithography and RIE. We measure quantum Hall effect under high magnetic field at low temperature by using He4 gas closed loop cryostat.

• 산업융합연구
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• 제20권10호
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• pp.131-136
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• 2022

본 연구는 가족 내 의사결정 태도가 청소년의 인권의식에 미치는 영향에서 자아존중감의 매개효과를 검증하는 것이다. 연구의 목적 달성을 위해 2018년 한국청소년정책 연구원에서 조사한 한국아동·청소년 인권실태조사 자료를 활용하여 분석하였다. 본 자료의 전체 응답자 중 아르바이트 경험이 있는 청소년 693명을 추출한 뒤, 결측치 및 이상치, 가중치를 제거하여 총 511명을 최종 연구대상으로 선정하였다. 측정 변인 간 영향력과 매개효과 검증을 위해 SPSS WIN 25.0 프로그램을 사용하였다. 분석결과, 가족 내 의사결정 태도가 청소년의 인권의식에 미치는 영향에서 자아존중감의 부분 매개 효과를 확인하였다. 또한, 자아존중감의 매개효과 유의성 확인을 위해 Sobel Test를 실시하였다. 본 연구결과를 토대로 부모-자녀 간의 바람직한 의사소통과 인권의식 고취 및 자아존중감 증진을 위한 사회복지개입실천의 필요성을 제언하였다.

• 문화기술의 융합
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• 제10권3호
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• pp.259-265
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• 2024

본 논문은 기업이 갖는 인적자원개발(HRD)에 대한 의지, 인적자원개발(HRD) 시행수준이 기업의 내외적 경쟁력에 미치는 효과와 각 변수 간의 관계에 대한 조직몰입의 매개효과에 대하여 분석하였다. 연구모형은 기업의 인적자원개발 의지와 인적자원개발 실행수준이 기업의 내외적 경쟁력에 직접적으로 긍정적인 영향을 미칠 것으로 가정하였다. 그리고 인적자원개발 의지와 인적자원개발 시행수준이 조직몰입을 통해 기업경쟁력에 미치는 간접효과가 있을것으로 설정하였다. 분석에 필요한 자료는 한국직업능력개발원(KRIVET)이 시행한 인적자본기업패널 Ⅱ(HCCP Ⅱ) 조사(2차)에서 수집된 9,516명 종업원의 자료를 기업수준(500개 기업)으로 통합하여 활용하였다. 본 연구모형을 분석하는데에는 구조방정식 분석을 사용하였다. 분석결과에 따르면, 기업의 인적자원개발 의지와 인적자원개발 시행수준이 높을수록 기업의 내v외적 경쟁력이 높아지는 효과가 있는 것을 확인하였다. 또한 부트스트래핑을 이용한 간접효과 분석에서는 인적자원개발 의지와 인적자원개발 시행수준이 모두 조직몰입을 통 간접효과가 유의한 것으로 나타났다.

• 동의생리병리학회지
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• 제23권1호
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• pp.199-205
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• 2009

It is unclear whether Fructus ligustri Lucidi (FLL) extract anti-proliferative effect in human glioma cells. The present study was therefore undertaken to examine the effect of FLL on cell viability and to determine the underlying mechanism in A172 human glioma cells. Cell viability and cell death were estimated by MTT assay and trypan blue exclusion assay, respectively. Apoptosis was measured by Annexin-V binding assay and cell cycle analysis. Activation of kinases and caspase-3 was estimated by Western blot analysis. FLL resulted in apoptotic cell death in a dose- and time-dependent manner. FLL-induced cell death was not associated with reactive oxygen species generation. Western blot analysis showed that FLL treatment caused down-regulation of PI3K/Akt pathway, but not ERK. The PI3K/Akt inhibitor LY984002 sensitized the FLL-induced cell death and overexpression of Akt prevented the cell death. FLL induced caspase-3 activation and the FLL-induced cell death was prevented by caspase inhibitors. These findings indicate that FLL results in a caspase-dependent cell death through a P13K/Akt pathway in human glioma cells. These data suggest that FLL may serve as a potential therapeutic agent for malignant human gliomas.

• 한국환경성돌연변이발암원학회지
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• 제23권1호
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• pp.35-40
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• 2003

Polycyclic aromatic hydrocarbons (PAH) are frequently detected in food, water, soil, and sediment and are widespread environmental pollutants formed by the incomplete combustion of fossil fuels, woods and other organic matter. PAHs are considered to be probable human carcinogens. The mechanism of action of PAHs has been studied extensively, however it is not clear how PAHs turn on CYP1A1 in human breast cancer. Our laboratory have been studied the effect of PAHs in the human breast cancer cell MCF7. In this study, we examined the ZR-75-1 human breast cancer cells as a new system to evaluate bioactivity of PAHs. ZR-75-1 human breast cancer cell line responses to estrogen and progesteron. We have been able to estbilish long term culture system of this cells then used for the study to observe the effect of PAHs. We demonstrate that PAHs induced the CYP1A1 promoter and 7-ethoxyresolufin O-deethylase (EROD) activity in a concentration-dependant manner. RT-PCR analysis indicated that PAHs significantly up-regulate the level of CYP1A1 mRNA. Some of PAHs showed stronger stimulatory effect on CYP1 gene expression than TCDD. Apparently, ZR-75-1 cells have Aryl hydrocarbon receptors, therefore it would be good experimental tool to study the cross-talk between PAHs and steroid actions.

• IMMUNE NETWORK
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• 제11권1호
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• pp.42-49
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• 2011

Background: In this study, we have investigated the effect of Korean red ginseng (KRG) extracts on the production of TNF-${\alpha}$ and IL-8 in human keratinocytes. Also, to examine the antioxidative effect of red ginseng extracts, free radical scavenging activity and superoxide dismutase (SOD) activity in human dermal fibroblasts was measured. Methods: To investigate the effect of KRG in atopic dermatitis, we measured the level of TNF-${\alpha}$ and IL-8 secretion in LPS-stimulated human keratinocytes after the treatment of KRG extracts using enzyme-linked immunosorbent assay. Anti-oxidative activity was investigated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and SOD activity. Results: The stimulation of human keratinocytes with KRG extracts shifted the LPS-induced cytokine secretion toward a more immunosuppressive response. KRG dose-dependently decreased TNF-${\alpha}$ and IL-8 production in HaCaT cells and a significant inhibition of TNF-${\alpha}$ was shown when cells were treated with 500 and $1,000{\mu}g/ml$ of KRG extracts. Additionally, KRG extracts showed DPPH radical scavenging and SOD activity in a dose-dependent manner. Particularly, SOD activities of concentrations higher than $60{\mu}g/ml$ of KRG extracts were significantly different in human dermal fibroblast cells. Conclusion: Based on this study, KRG extracts may be a useful immunosuppressive agent in the treatment of atopic dermatitis.

• 한국안전학회지
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• 제31권3호
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• pp.60-67
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• 2016

The gas leak and explosion accident is able to give a fatal injury to nearby people from the explosion center and interest in effect of the explosion on the human body is increased. Accidents by Portable Butane Gas Range of a gas explosion accident occupy the most share. As a result, the injury on the human body frequently occur. However, It is situation that are experiencing difficulties in consequence analysis of explosion accidents owing to shortage of explosion power data and lack of research on the effect of the human body by the gas explosion. This paper acquire human injury data by performing the actual explosion experiment with Portable Butane Gas Range and evaluate power by explosion and effect of explosion on the human body to perform explosion simulation with LS-DYNA program. It is intended to contribute to the exact cause of the accident investigation and the same type of accident prevention.

• 동의생리병리학회지
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• 제20권1호
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• pp.88-92
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• 2006

Genistein was tested for chemopreventive potential against breast cancer by measuring the effect on proliferation of human breast cancer cells, human placental aromatase activity and cyclooxygenases-2 (COX-2) expression and activity, Genistein inhibited the growth of estrogen-independent MDA-MB-231 human breast cancer cell. However, there is no inhibitory effect of genistein on human placental aromatase activity. The expression of COX-2 was inhibited by genistein in Western blot analysis. Genistein significantly inhibited COX-2 activity at the concentrations of 10 (p<0.05), 25 (p<0.05) and 50 ${\mu}M$ (p<0.01). These results suggest that genistein may have breast cancer chemopreventive potential by inhibiting the growth of human breast cancer cell and expression and activity of COX-2.