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http://dx.doi.org/10.14374/HFS.2022.30.2.59

A study on the synergistic efficacy of Carthami flos in apoptosis of human gastric cancer by doxorubicin  

Kim, Byung Joo (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
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Herbal Formula Science / v.30, no.2, 2022 , pp. 59-66 More about this Journal
Abstract
Objectives : This study is to investigate whether Carthami flos exhibits a synergistic effect on the apoptotic effect of doxorubicin on human gastric cancer cells. Methods : We used AGS, a human gastric cancer cell line. To investigate the apoptotic efficacy of doxorubicin and Carthami flos, MTT and CCK-8 methods were used. To confirm apoptosis, cell cycle and mitochondrial membrane potential changes were confirmed. To investigate the mechanism of apoptosis, the reactive oxygen species (ROS) experiment was performed. Results : 1. Doxorubicin or Carthami flos induced cell death in the human gastric cancer cell line AGS. 2. Carthami flos showed a synergistic effect of cell death by doxorubicin. 3. The cell cycle and mitochondrial membrane potential changes revealed that cell death was apoptosis. 4. Apoptosis was related to reactive oxygen species (ROS) generation. Conclusions : This result shows the anticancer synergistic effect of Carthami flos in gastric cancer cells, and is considered to be an important basis for the development of anticancer drugs for Carthami flos.
Keywords
Carthami flos; Doxorubicin; Human Gastric Cancer Cell; Apoptosis;
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