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Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence

  • Zheng, Guochao;Alsarakibi, Muhamd;Liu, Yuanjia;Hu, Wei;Luo, Qin;Tan, Liping;Li, Guoqing
    • Parasites, Hosts and Diseases
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    • v.52 no.3
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    • pp.299-304
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    • 2014
  • This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), ${\beta}$-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates.

공기 유량의 시간 변화에 따른 $U_3O_8$ 타원입자에 대한 거동 특성 해석

  • Kim, Yeong-Hwan;Jeong, Jae-Hu;Lee, Hyo-Jik;Park, Byeong-Seok;Yun, Ji-Seop
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2007.11a
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    • pp.305-306
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    • 2007
  • ACP(Advanced Spent Fuel Conditioning Process)의 금속전환로에 $U_3O_8$을 공급하기 위하여 20 kgHM/batch의 $UO_2$ 펠릿(pellets)을 처리할 수 있는 건식분말화 장치가 개발되고있다. 건식분말화 장치는 500 $^{\circ}C$온도에서 공기를 공급하여 일정한 입도범위의 균질한 $U_3O_8$을 만든다. 이런 건식 분말화 장치의 효율을 높이기 위해서는 반웅로에 불어 넣어주는 공기의 유량을 증가시킬 필요가 있다. 하지만 공기와 반응하여 생성되는 $U_3O_8$ 입자는 그 크기가 최소 3 ${\mu}$m 정도로 매우 미세하여,반응로 출구를 통해 외부로 빠져나갈 가능성 이있다. 이를 방지하기 위해 분말화 장치 출구 바깥에는 필터가 설치되어 있으나 공기와 함께 $U_3O_8$ 입자가 계속해서 빠져 나갈 경우 입자로 인해 필터가 막혀 제 기능을 할 수 없게 된다. 따라서 건식 분말화 장치는 미세한 $U_3O_8$ 입자가 반응로 밖으로 빠져나가지 않도록 입구에서의 공기 유량을 일정 수준 이하로 조절해주는 것이 필요하다. 이 연구의 목적은 초기 유량으로부터 유량을 점점 증가시키면서 시간변화에 따른 입자 거동 특성을 해석하며, 결과로부터 주어진 크기의 타원입자에 대해 최대 허용 공기 유량을 결정하고자한다. 이 해석을 위해 유동과 입자를 동시에 해석할 수 있는 ANSYS-CFX 5.7.1과 ANSYS-CFX 10.0 두 가지의 소프트웨어가 사용되었다. 해석 결과를 바탕으로 좀더 정확한 유량 한계치 계산을 위해 추가로 수행되어야 할 해석에 대해 제안하였다.

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Corrosion and Strength Degradation Characteristics of 1.25Cr-0.5Mo Steel under SO2 Gas Environment (SO2 가스 환경 하에서 1.25Cr-0.5Mo 강의 부식 및 강도 저하 특성)

  • Jung, Kwang-Hu;Kim, Seong-Jong
    • Journal of the Korean institute of surface engineering
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    • v.51 no.3
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    • pp.149-156
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    • 2018
  • The corrosion and strength degradation characteristics of 1.25Cr-0.5Mo steels were studied under $650^{\circ}C$ in $76%N_2+6%O_2+16%CO_2+2%SO_2$ gas condition up to 500 hrs. Corroded specimens were characterized by weight gain, scanning electron microscope(SEM), energy dispersive X-ray spectroscopy(EDS), and X-ray diffraction(XRD). The tensile test was conducted to evaluate the mechanical strength and fracture mode with corrosion at high temperature. As the results of the experiments, thick Fe-rich oxide layers over $200{\mu}m$ were formed on the surface within 500 hrs. The thick oxide layers are formed with reduction of the cross-sectional area of the specimens. Thus, the strength tended to decrease with reduction of the cross-sectional area.

Evaluation of Ground Effective Thermal Conductivity and Borehole Effective Thermal Resistance from Simple Line-Source Model (단순 선형열원 모델을 이용한 지중 유효 열전도도와 보어홀 유효 열저항 산정)

  • Sohn, Byong-Hu
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.19 no.7
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    • pp.512-520
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    • 2007
  • The design of a ground-source heat pump system includes specifications for a ground loop heat exchanger where the heat transfer rate depends on the effective thermal conductivity of the ground and the effective thermal resistance of the borehole. To evaluate these heat transfer properties, in-situ thermal response tests on four vertical test boreholes with different grouting materials were conducted by adding a monitored amount of heat to circulating water. The line-source method is applied to the temperature rise in an in-situ test and extended to also give an estimate of borehole effective thermal resistance. The effect of increasing thermal conductivity of the grouting materials from 0.818 to $1.104W/m^{\circ}C$ resulted in overall increases in effective thermal conductivity by 15.8 to 56.3% and reductions in effective thermal resistance by 13.0 to 31.1%.

Non-isothermal Decomposition Kinetics of a New High-energy Organic Potassium Salt: K(DNDZ)

  • Xu, Kangzhen;Zhao, Fengqi;Song, Jirong;Ren, Xiaolei;Gao, Hongxu;Xu, Siyu;Hu, Rongzu
    • Bulletin of the Korean Chemical Society
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    • v.30 no.10
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    • pp.2259-2264
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    • 2009
  • A new high-energy organic potassium salt, 2-(dinitromethylene)-1,3-diazepentane potassium salt K(DNDZ), was synthesized by reacting of 2-(dinitromethylene)-1,3-diazepentane (DNDZ) and potassium hydroxide. The thermal behavior and non-isothermal decomposition kinetics of K(DNDZ) were studied with DSC, TG/DTG methods. The kinetic equation is $\frac{d{\alpha}}{dT}$ = $\frac{10^{13.92}}{\beta}$3(1 - $\alpha$[-ln(1 - $\alpha$)]$^{\frac{2}{3}}$ exp(-1.52 ${\times}\;10^5$ / RT). The critical temperature of thermal explosion of K(DNDZ) is $208.63\;{^{\circ}C}$. The specific heat capacity of K(DNDZ) was determined with a micro-DSC method, and the molar heat capacity is 224.63 J $mol^{-1}\;K^{-1}$ at 298.15 K. Adiabatic time-to-explosion of K(DNDZ) obtained is 157.96 s.

Origin of Multiple Conductance Peaks in Single-Molecule Junction Experiments

  • Park, Min Kyu;Kim, Hu Sung;Kim, Yong-Hoon
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.654-654
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    • 2013
  • One of the most important yet unresolved problems in molecular electronics is the controversy over the number and nature of multiple conductance peaks in single-molecule junctions. Currently, there are three competing explanations of this observation: (1) manifestation of different molecule-electrode contact geometries, (2) formation of gauche defects within the molecular core, (3) involvement of different electrode surface orientations [1]. However, the exact origin of multiple conductance peaks is not yet fully understood, which indicates our incomplete understanding of the scientifically as well as techno-logically important organic-metal contacts. To theoretically resolve this problem, we previously applied a multiscale computational approach that combines force fields molecular dynamics (FF MD), density functional theory (DFT), and matrix Green's function (MGF) calculations [2] to a thermally fluctuating haxanedithiol (C6DT) molecule stretched between flat Au(111) electrodes, but could observe only a single conductance peak [3]. In this presentation, using DFT geometry optimizations and MGF calculations, we consider molecular junctions with more realistic molecule-metal contact conformations and Au(111) electrode surface directions. We also conduct DFT-based molecular dynamics for the highly stretched junction models to confirm our conclusion. We conclude that the S-Au coordination number should be the more dominant factor than the electrode surface orientation.

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Construction and Characterization of an Enhanced GFP-Tagged TIM-1 Fusion Protein

  • Qing, Jilin;Xiao, Haibing;Zhao, Lin;Qin, Guifang;Hu, Lihua;Chen, Zhizhong
    • Journal of Microbiology and Biotechnology
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    • v.24 no.4
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    • pp.568-576
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    • 2014
  • TIM-1 (also known as KIM-1 and HAVcr-1) is a type I transmembrane glycoprotein member of the TIM family that may play important roles in innate and adaptive immune responses. The overexpression of proteins associated with membrane proteins is a major obstacle to overcome in studies of membrane protein structures and functions. In this study, we successfully coupled the overexpression of the TIM-1 protein with a C-terminal enhanced green fluorescent protein (GFP) tag in Escherichia coli. To the best of our knowledge, this report is the first to describe the overexpression of human TIM-1 in E. coli. The purified TIM-1-EGFP fusion protein recognized and bound directly to apoptotic cells and did not to bind to viable cells. Furthermore, we confirmed that the interactions of TIM-1-EGFP with apoptotic cells were blocked by TIM-1-Fc fusion proteins. This fusion protein represents a readily obtainable source of biologically active TIM-1 that may prove useful in future studies of human TIM-1.

Evaluation of Effective Thermal Conductivity and Thermal Resistance in Ground Heat Exchanger Boreholes (지중 열교환기 보어홀에서의 유효 열전도도 및 열저항 산정)

  • Sohn Byong Hu;Shin Hyun-Joon;Park Seong-Koo
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.17 no.8
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    • pp.695-703
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    • 2005
  • The objective of this study is to determine the effective thermal conductivity and thermal resistance values in test boreholes with three different fill materials. To evaluate these heat transfer properties, in-situ tests on four vertical boreholes were conducted by adding a monitored amount of heat to water over various test lengths. Two parameter estimation models, line-source and numerical one-dimensional models, for evaluation of thermal response test data were compared when applied on the same four data sets. Results show that the average thermal conductivity deviation between measured data and these two models is in the range of $3.03\%$ to $4.45\%$. The effect of increasing grout thermal conductivity from 1.34 to 1.82 $W/m^{\circ}C$ resulted in overall increases in effective formation thermal conductivity by $11.1\%$ to $51.9\%$ and reductions in borehole thermal resistance by $11.6\%$ to $26.1\%$.

Triterpenoids and Sterones from the Stem Bark of Ailanthus altissima

  • Zhou, Xiao-Jiang;Xu, Min;Li, Xue-Song;Wang, Yue-Hu;Gao, Ye;Cai, Rui;Cheng, Yong-Xian
    • Bulletin of the Korean Chemical Society
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    • v.32 no.1
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    • pp.127-130
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    • 2011
  • One new tirucallane-type triterpenoid, alianthusaltinin A (1), one new $C_{29}$ sterone, alianthaltone A (2), and 12 known compounds have been isolated from the stem bark of Ailanthus altissima. The structures of new compounds were identified by means of spectroscopic methods. Compound 3 was isolated from natural sources for the first time, and compounds 4, 5, and 9 were isolated from this plant for the first time.

Fabrication of transparent conductive oxides for Dye-sensitized solar cell application (염료 태양전지용 투명 전도설 박막제작 및 특성 고찰)

  • Hu, Jong-Hyun;Kim, Ji-Hoon;Sung, Youl-Moon;Park, Cha-Soo
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 2008.10a
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    • pp.205-210
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    • 2008
  • Titanium-doped indium oxide (ITiO) films were prepared on soda-lime glass substrate using a magnetic null discharge (MND) sputter source. The ITiO thin films containing 10wt.% Ti showed the minimum resistivity of $\rho=5.5{\times}10^{-3}{\Omega}-cm$. The optical transmittance increases from 70% at 450 nm to 80% at 700 nm in visible spectrum. Photoelectron peaks for In 3d, Ti 2p, O 1s and C1s were detected for the ITiO film in the binding energy range of 0 to 1100 eV. The surface roughness of the sample showed a change from 10 nm to 50 nm. The ITiO film used for TCO layer of DSCs exhibited an energy conversion efficiency of about 3.8% at light intensity of 100 mW/$cm^2$.

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