Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence

  • Zheng, Guochao (College of Veterinary Medicine, South China Agricultural University) ;
  • Alsarakibi, Muhamd (College of Veterinary Medicine, South China Agricultural University) ;
  • Liu, Yuanjia (College of Veterinary Medicine, South China Agricultural University) ;
  • Hu, Wei (College of Veterinary Medicine, South China Agricultural University) ;
  • Luo, Qin (College of Veterinary Medicine, South China Agricultural University) ;
  • Tan, Liping (College of Veterinary Medicine, South China Agricultural University) ;
  • Li, Guoqing (College of Veterinary Medicine, South China Agricultural University)
  • Received : 2014.02.21
  • Accepted : 2014.05.14
  • Published : 2014.06.30
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Abstract

This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), ${\beta}$-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates.

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References

  1. Cooper MA, Sterling CR, Gilman RH, Cama V, Ortega Y, Adam RD. Molecular analysis of household transmission of Giardia lamblia in a region of high endemicity in Peru. J Infect Dis 2010; 202: 1713-1721. https://doi.org/10.1086/657142
  2. Thompson RCA, Hopkins RM, Homan WL. Nomenclature and genetic groupings of Giardia infecting mammals. Parasitol Today 2000; 16: 210-213. https://doi.org/10.1016/S0169-4758(99)01624-5
  3. Monis PT, Caccio SM, Thompson RCA. Variation in Giardia: towards a taxonomic revision of the genus. Trends Parasitol 2009; 25: 93-100. https://doi.org/10.1016/j.pt.2008.11.006
  4. Vanni I, Caccio SM, van Lith L, Lebbad M, Svard SG, Pozio E, Tosini F. Detection of Giardia duodenalis assemblages A and B in human feces by simple, assemblage-specific PCR assays. PLoS Negl Trop Dis 2012; 6: e1776. https://doi.org/10.1371/journal.pntd.0001776
  5. Bertrand I, Albertini L, Schwartzbrod J. Comparison of two target genes for detection and genotyping of Giardia lamblia in human feces by PCR and PCR-restriction fragment length polymorphism. J Clin Microbiol 2005; 43: 5940-5944. https://doi.org/10.1128/JCM.43.12.5940-5944.2005
  6. Caccio SM, Ryan U. Molecular epidemiology of giardiasis. Mol Biochem Parasitol 2008;160: 75-80. https://doi.org/10.1016/j.molbiopara.2008.04.006
  7. Lasek-Nesselquist E, Welch DM, Sogin ML. The identification of a new Giardia duodenalis assemblage in marine vertebrates and a preliminary analysis of G. duodenalis population biology in marine systems. Int J Parasitol 2010; 40: 1063-1074. https://doi.org/10.1016/j.ijpara.2010.02.015
  8. Feng Y, Xiao L. Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clin Microbiol Rev 2011; 24: 110-140. https://doi.org/10.1128/CMR.00033-10
  9. Yong TS, Park SJ, Hwang UW, Yang HW, Lee KW, Min DY, Rim HJ, Wang Y, Zheng F. Genotyping of Giardia lamblia isolates from humans in China and Korea using ribosomal DNA sequences. J Parasitol 2000; 86: 887-891. https://doi.org/10.1645/0022-3395(2000)086[0887:GOGLIF]2.0.CO;2
  10. Wang R, Zhang X, Zhu H, Zhang L, Feng Y, Jian F, Ning C, Qi M, Zhou Y, Fu K, Wang Y, Sun Y, Wang Q, Xiao L. Genetic characterizations of Cryptosporidium spp. and Giardia duodenalis in humans in Henan, China. Exp Parasitol 2011; 127: 42-45. https://doi.org/10.1016/j.exppara.2010.06.034
  11. Li J, Zhang P, Wang P, Alsarakibi M, Zhu H, Liu Y, Meng X, Li J, Guo J, Li G. Genotype identification and prevalence of Giardia duodenalis in pet dogs of Guangzhou, Southern China. Vet Parasitol 2012; 188: 368-371. https://doi.org/10.1016/j.vetpar.2012.04.004
  12. Sprong H, Caccio SM, van der Giessen JWB, Network Z. Partners identification of zoonotic genotypes of Giardia duodenalis. PLoS Negl Trop Dis 2009; 3: e558. https://doi.org/10.1371/journal.pntd.0000558
  13. Covacin C, Aucoin DP, Elliot A, Thompson RCA. Genotypic characterisation of Giardia from domestic dogs in the USA. Vet Parasitol 2011; 177: 28-32. https://doi.org/10.1016/j.vetpar.2010.11.029
  14. Hopkins RM, Meloni BP, Groth DM, Wetherall JD, Reynoldson JA, Thompson RCA. Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and dogs living in the same locality. J Parasitol 1997; 83: 44-51. https://doi.org/10.2307/3284315
  15. Sulaiman IM, Jiang JL, Singh A, Xiao LH. Distribution of Giardia duodenalis genotypes and subgenotypes in raw urban wastewater in Milwaukee, Wisconsin. Appl Environ Microbiol 2004; 70: 3776-3780. https://doi.org/10.1128/AEM.70.6.3776-3780.2004
  16. Tangtrongsup S, Scorza V. Update on the diagnosis and management of Giardia spp. infections in dogs and cats. Top Companion Anim M 2010; 25: 155-162. https://doi.org/10.1053/j.tcam.2010.07.003
  17. Epe C, Rehkter G, Schnieder T, Lorentzen L, Kreienbrock L. Giardia in symptomatic dogs and cats in Europe- results of a European study. Vet Parasitol 2010; 173: 32-38. https://doi.org/10.1016/j.vetpar.2010.06.015
  18. Scorza AV, Ballweber LR, Tangtrongsup S, Panuska C, Lappin MR. Comparisons of mammalian Giardia duodenalis assemblages based on the beta-giardin, glutamate dehydrogenase and triose phosphate isomerase genes. Vet Parasitol 2012; 189: 182-188. https://doi.org/10.1016/j.vetpar.2012.04.032
  19. Ballweber LR, Xiao L, Bowman DD, Kahn G, Cama VA. Giardiasis in dogs and cats: update on epidemiology and public health significance. Trends Parasitol 2010; 26: 180-189. https://doi.org/10.1016/j.pt.2010.02.005
  20. Huey CS, Mahdy MAK, Al-Mekhlafi HM, Nasr NA, Lim YAL, Mahmud R, Surin J. Multilocus genotyping of Giardia duodenalis in Malaysia. Infect Genet Evol 2013; 17: 269-276. https://doi.org/10.1016/j.meegid.2013.04.013
  21. Hussein AIA, Yamaguchi T, Nakamoto K, Iseki M, Tokoro M. Multiple-subgenotype infections of Giardia intestinalis detected in Palestinian clinical cases using a subcloning approach. Parasitol Int 2009; 58: 258-262. https://doi.org/10.1016/j.parint.2009.04.002
  22. Lebbad M, Petersson I, Karlsson L, Botero-Kleiven S, Andersson JO, Svenungsson B, Svard SG. Multilocus genotyping of human Giardia isolates suggests limited zoonotic transmission and association between assemblage B and flatulence in children. PLoS Negl Trop Dis 2011; 5: e1262. https://doi.org/10.1371/journal.pntd.0001262
  23. Beck R, Sprong H, Pozio E, Caccio SM. Genotyping Giardia duodenalis isolates from dogs: lessons from a multilocus sequence typing study. Vector-Borne Zoon Dis 2012; 12: 206-213. https://doi.org/10.1089/vbz.2011.0751

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