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http://dx.doi.org/10.3347/kjp.2014.52.3.299

Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence  

Zheng, Guochao (College of Veterinary Medicine, South China Agricultural University)
Alsarakibi, Muhamd (College of Veterinary Medicine, South China Agricultural University)
Liu, Yuanjia (College of Veterinary Medicine, South China Agricultural University)
Hu, Wei (College of Veterinary Medicine, South China Agricultural University)
Luo, Qin (College of Veterinary Medicine, South China Agricultural University)
Tan, Liping (College of Veterinary Medicine, South China Agricultural University)
Li, Guoqing (College of Veterinary Medicine, South China Agricultural University)
Publication Information
Parasites, Hosts and Diseases / v.52, no.3, 2014 , pp. 299-304 More about this Journal
Abstract
This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), ${\beta}$-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates.
Keywords
Giardia duodenalis; dog; multi-locus sequence typing; Guangdong; China;
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