Proceedings of the Korean Society for Applied Microbiology Conference
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2001.06a
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pp.116-117
/
2001
A thermotolerant bacterium, designated as PHS1, was isolated from a hot spring in Pohang, Korea, on the basis of its ability to grow on BTEX as a sole carbon source. We cloned and sequenced the entire BTEX-degrading pathway genes of PHS1 and found that two multicomponent mono-oxygenases together with meta-pathway genes are responsible for the BTEX biodegradation.
Transactions of the Korean Society of Mechanical Engineers A
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v.38
no.12
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pp.1373-1377
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2014
Hot-stamping is a method of obtaining ultrahigh-strength steel by simultaneously forming and cooling boron steel in a press die after it has been heated at $900^{\circ}C$ or above. After heat treatment, boron steel has a strength of 1500 MPa or more. This material ensures a high level of quality because it overcomes the spring-back phenomenon, which is a problem associated with high-strength steel materials, and the degree of dimensional precision is improved by 90 or more because of the good formability compared with existing types of steel. In this study, the welding characteristics were identified through the butt and lap welding of hot-stamped steel using a disk laser. Full penetration was obtained at a faster speed with butt welding compared to lap welding, and a white band was observed in every specimen.
This paper summarizes the history of geothermal research in Korea since 1920s and also describes the present status of research on heat flow, origin of thermal waters and geothermal exploitation and utilization. Geothermal research in Korea has been mainly related with hot spring investigation until 1970s. 1t was not until 1980s before heat flow study became continuous by research institute and academia and first nation-scale geothermal gradient map and heat flow map were published in 1996. Also in 1990s, geochemical isotope analysis of Korean hot spring waters and measurements of heat production rate of some granite bodies were made. Attempts to develop and utilize the deep geothermal water has been tried from early 1990s but field scale exploitations for geothermal water was activated in 2000s. Considering recent increase of demands on both deep and shallow geothermal energy utilization, outlook on future goethermal research and development is encouraging.
As Gangwon-do Province needs an environmental-friendly alternative tourism according to low carbon green growth, this study analyzes specialized fields in green health industry, selects seven specialized fields in green health industry that is fit to Gangwon-do Province, and suggests a development strategy by conducting recognition surveys on preference and impotence of the specialized fields. Surveys and interviews are conducted of 106 students at Green Health Industry Education Center which is operating by Kwandong University and 84 persons who work at medical institutions and tourism related companies in Ganwondo. The surveys and interviews were completed from November 5 to November 19 in 2010, determined factors through a factor analysis when they have many questions, and analyzed by using SPSS 14.0. Out of the seven specialized fields, rest & recuperation field is highly recognized. In order to have competitive edge compared to other self-governing provinces, Ganwon-do Province should prioritize rest and recuperation field. A healthcare program in the green health industry specific to Ganwon-do Province includes hot springs and spa programs as a priority. Gangwon-do's hot springs are deemed to be competitive resources as global medical tourists prefer spa and sauna healthcare programs, while the province promotes its spring resources and builds medical tourism infrastructures. Gangwon-do Province can promote a medical tourism industry that is well suitable for the strength and characteristics of the province. It might pursue oriental medicine therapy tourism, which is related to a recuperating medical service that uses both oriental and western medicine. It can also run such programs as forest bathing (oxygen road), spring and spa, and sea water treatment, which are the specialized fields in green health industry with respect to recreation and healthcare.
A xylanase-producing thermophilic strain, Geobacillus sp. TC-W7, was isolated from a hot spring in Yongtai (Fuzhou, China). Subsequently, the xylanase gene that encoded 407 amino acids was cloned and expressed. The recombinant xylanase was purified by GST affinity chromatography and exhibited maximum activity at $75^{\circ}C$ and a pH of 8.2. The enzyme was active up to $95^{\circ}C$ and showed activity over a wide pH range of 5.2 to 10.2. Additionally, the recombinant xylanase showed high thermostability and pH stability. More than 85% of the enzyme's activity was retained after incubation at $70^{\circ}C$ for 90 min at a pH of 8.2. The activity of the recombinant xylanase was enhanced by treatment with 10 mM enzyme inhibitors (DDT, Tween-20, 2-Me, or TritonX-100) and was inhibited by EDTA or PMSF. Its functionality was stable in the presence of $Li^+$, $Na^+$, and $K^+$, but inhibited by $Hg^{2+}$, $Ni^{2+}$, $Co^{2+}$, $Cu^{2+}$, $Zn^{2+}$, $Pb^{2+}$, $Fe^{3+}$, and $Al^{3+}$. The functionality of the crude xylanase had similar properties to the recombinant xylanase except for when it was treated with $Al^{2+}$ or $Fe^{2+}$. The enzyme might be a promising candidate for various industrial applications such as the biofuel, food, and paper and pulp industries.
The rapid development of the poultry industry has led to the production of large amounts of manure, which produce substances like hydrogen sulfide ($H_2S$) that contribute to odor pollution. $H_2S$ is a highly undesirable gas component and its removal from the environment is therefore necessary. Sulfur-oxidizing bacteria (SOB) are widely known to remove contaminating $H_2S$ due to their ability to oxidize reduced sulfur compounds. In this study, three potential SOB (designated AH18, AH25, and AH28) that were previously isolated from a hot spring in Malaysia were identified by 16S rRNA gene analysis. Laboratory-scale biological deodorization experiments were conducted to test the performance of the three isolates-in the form of pure or mixed cultures, with the cells immobilized onto alginate as a carrier-in reducing the $H_2S$ from chicken manure. On the basis of 16S rRNA phylogenetic analysis, isolate AH18 was identified as Pseudomonas sp., whereas isolates AH25 and AH28 were identified as Achromobacter sp. The most active deodorizing isolate was AH18, with an $H_2S$ reduction rate of 74.7% (p < 0.05). Meanwhile, the reduction rates for isolates AH25 and AH28 were 54.2% and 60.8% (p > 0.05), respectively. However, the $H_2S$ removal performance was enhanced in the mixed culture, with a reduction rate of 81.9% (p < 0.05). In conclusion, the three potential SOB isolates were capable of reducing the $H_2S$ from chicken manure in the form of a pure culture immobilized on alginate, and the reduction performance was enhanced in the mixed culture.
Thermal effluent of the hot spring has long been a field of interest in the relationship between temperature gradient and freshwater algae in geology, limnology and aquatic ecology throughout the world. On the other hand, many artificial hot springs have been developed in Korea, but the research on them has not been still active. This study was performed every month from December 2015 to September 2016, to elucidate the spatiotemporal effects of thermal wastewater effluent (TWE) on the ecosystem of benthic algal assemblage in four stations(BSU (upstream), HSW (hot spring wastewater outlet), BSD1~2 (downstream)) of the upstream reach of the Buso Stream, a tributary located in the Hantan River basin. During the survey, the influencing distance of temperature on TWE was <1.0 km, and it was the main source of N P nutrients at the same time. The effects of TWE were dominant at low temperature and dry season (December~March), but it was weak at high temperature and wet season (July~September), reflecting some seasonal characteristics. Under these circumstances, the attached algal communities were identified to 59 genera and 143 species. Of these, the major phylum included 21 genera 83 species of diatoms(58.0%), 9 genera 21 species of blue-green algae (14.7%) and 25 genera 32 species of green algae (22.4%), respectively. The spatiotemporal distribution of them was closely related to water temperature ($5^{\circ}C$ and $15^{\circ}C$) and current ($0.2m\;s^{-1}$ and $0.8m\;s^{-1}$). In the basic environment maintaining a high water temperature throughout the year round, the flora favoring high affinity to $PO_4$ in the water body or preferring stream habitat of abundant $NO_3-PO_4$ was dominant. As a result, when compared with the outcomes of previous algal ecology studies conducted in Korea, the Buso Stream was evaluated as a serious polluted state due to persistent excess nutrient supply and high thermal pollution throughout the year round by TWE. It can be regarded as a dynamic ecosystem in which homogeneity (Summer~Autumn) and heterogeneity (Winter~Spring) are repeated between upstream and downstream.
The sewage and wastewater (SAW) are a well-known major source of eutrophication and greentide in freshwaters and also a potential source of thermal pollution; however, there were few approaches to thermal effluent of SAW in Korea. This study was performed to understand the behavioral dynamics of the thermal effluents and their effects on the water quality of the connected streams during winter season, considering domestic sewage, industrial wastewater and hot spring wastewater from December 2015 to February 2016. Sampling stations were selected the upstream, the outlet of SAW, and the downstream in each connected stream, and the water temperature change was monitored toward the downstream from the discharging point of SAW. The temperature effect and its range of SAW on the stream were dependent not only on the effluent temperature and quantity but also on the local air temperature, water temperature and stream discharge. The SAW effects on the stream water temperature were observed with temperature increase by $2.1{\sim}5.8^{\circ}C$ in the range of 1.0 to 5.5 km downstream. Temperature effect was the greatest in the hot spring wastewater despite of small amount of effluent. The SAW was not only related to temperature but also to the increase of organic matter and nutrients in the connected stream. The industrial wastewater effluent was discharged with high concentration of nitrogen, while the hot spring wastewater was high in both phosphorus and nitrogen. The difference between these cases was due to with and without chemical T-P treatment in the industrial and the hot spring wastewater, respectively. The chlorophyll-a content of the attached algae was high at the outlet of SAW and the downstream reach, mostly in eutrophic level. These ecological results were presumably due to the high water temperature and phosphorus concentration in the stream brought by the thermal effluents of SAW. These results suggest that high temperature of the SAW needs to be emphasized when evaluating its effects on the stream water quality (water temperature, fertility) through a systematized spatial and temporal investigation.
The trend of the concept for modern medicine is gradually forwarding to preventive medicine from therapeutic medical science. One of the most remarkable characteristics of oriental medicine is that it attaches greater importance to preventive medicine scientifically. The basic theory of oriental medicine principally takes roots in Huang Di Nei Jing and it is Si Qi Tian Shen Da Lun that is deep-rooted in the principle of "growing life" grounded in theory of unity of heaven and man of oriental medicine. Therefore having translated annotation of 〈Si Qi Tian Shen Da Lun·Huang Di Nei Jing Su Wen〉 which is quoted frequently and using it for appendix and comparing each views of annotators, I would like to state the results of dissertation as below. 1. Si Qi Tian Shen of title of the paper means that it keeps our health and prevent diseases in advance to control our mind and rhythm of life to the change of Yin and Yang - that is, the grower Yin, the looser Yang - following four seasons of nature environment. 2. The summary of this dissertation is that spring means things newly sprout from old ones, things become profuse, beautiful and brilliant in the summer. Autumn stands for things is harvested and allocated evenly and finally things is closed and stored. That is, in the spring and summer. If one break this principle of growth, diseases are followed after this. Therefore an excellent physician should handle diseases before they are attacked not remedy them after outbreak of diseases. It is said that a sage governs the nation before it is put into confusion. 3. These four terms standing for each season describe definitely and realistically rural life in the agricultural environment, in other words, spouting in spring, growing in summer, harvesting in autumn, storing in winter. Going with the current of the times, they have developed to theoretical concept of getting, growth, harvest, store so that implied the principle of growth for four seasons. 4. It means in a concrete way "Yang would grow in the spring and summer and Yin would grow in autumn and winter" as follow. That is, when the day is long, things act energetically and emit the warmth of life and expand the vigor of growth. On the other hand, when the night is long, things lessen their activity to protect the warmth of life and to preserve the sprit of growth. In addition, we should be concerned about the work outward and try to concretely fulfill plan of business in spring and summer. It is in autumn and winter when we should grow Yin. It does not mean that we simply grow cold yin in our body to build Yin. But according to annotators, for example, Wang Bing stated that we need to make things be cool in spring and summer, and be warm in autumn and winter for improvement. Zhang Jie Bin noted that things should not be cold in spring and summer and not be too hot to improve in autumn and winter. Those principles theorized to unify physiological status into the principle of changing of Yang and Yin in the nature and enriched the contents. In these principles, no problems are not found logically somewhat.
Bulb onion cultivation area has been restricted in southern part of Korea to avoid blotting and bulb division. The traditional culture method is transplanting bare-rooted plantlet into the field at the end of summer and harvesting at the beginning of next summer. The hot weather and weak plantlets occasionally causes unstable supply of onions in autumn. In order to enlarge cultivation area and to reduce culture period, long nursery system using plug tray and spring transplanting was tried. Forty cultivars collected from Korea and Japan were nursed using 200-plug tray and transplanted to the field in spring. Marketable yield was not related to the seedling size but lodging time. Cultivar of 'Hamasodachi' was lodged early and resulted low marketable yield. Cultivar of 'Cheonjudaego' was not lodged and yielded high but not in accordance with storability. Generally early lodged cultivars showed low storability. In order to avoid rainy harvesting season, cultivars requires excessive long time for lodging is not recommended for spring culture. Using plug nursery and spring transplanting, we successfully produced marketable onions in 3 months. But immediate using of the harvested onion is recommended. The storability of produced onions showed different result among cultivars, storage of spring onion was not recommended.
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