• Archives of Pharmacal Research
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• 제24권5호
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• pp.407-411
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• 2001

Reversed-phase high performance liquid chromatographic method was applied for the determination of eleutheroside B and E in the various Acanthopanax species collected In Korea. The stationary phase used was Zorbax 300 SB $C_{18}$ and a mobile phase program was used, which started at 6% acetonitrile for 2 min, and then a linear gradient was operated for the next 18 min to 17% acetonitrile at a flow rate of 1.0 ml/min. The column effluent was monitored at UV 210 nm. Identification was carried out by comparing the retention time and the LC/MS spectrum of each peak corresponding to eleutheroside B and E from sample with those of standards. In general, the contents of eleutheroside B and 1 in stems were higher than those In roots. Acanthopanax species could be classified into two groups based upon the contents of eleutheroside B and E: one group contains no or very little eleutheroside B and another contains both eleutheroside B and E.

• Archives of Pharmacal Research
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• 제29권1호
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• pp.96-101
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• 2006

Phytoestrogens have been used as a food supplement to prevent osteoporosis. The isoflavones in the phytoestrogens are daidzein, genistein and formononetin which are present in various herbs. This study examined the quantity of isoflavones in medicinal herbs, which can be used as a phytoestrogen supplement; soybean. These isoflavones were quantified using high performance liquid chromatography (HPLC) with a UV/VIS detector. The concentration of daidzein in Puerariae Radix was $10,436.16{\pm}2,143.83\;mg/kg$ of the dried herb, which was much higher than that extracted from soybeans, $341.47{\pm}18.96\;mg/kg$. The amount of genistein in Sophorae flavescentis Radix ($336.09{\pm}50.89mg/kg$) was approximately 11 times higher than that extracted from soybean ($30.03{\pm}7.17mg/kg$). The level of formononetin in Dalbergiae odoriferae Lignum, $2,189.14{\pm}136.46mg/kg$, was the highest among the herbs tested. The total isoflavone content of Puerariae Radix was approximately 30 times higher than that extracted from soybean. Therefore, plants from the family Leguminosae, particularly Puerariae Radix, can be a good source of phytoestrogens.

• 한국축산식품학회지
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• 제32권5호
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• pp.571-577
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• 2012

This study was carried out to develop an analytical method for the determination of vitamin D in infant formula. Vitamin D was determined by column-switching high-performance liquid chromatography (HPLC) equipped with a reversed phase column and UV detector after saponification and extraction of the formula with an organic solvent. A preseparation column ($C_8$), focusing column ($C_{18}$), analytical column ($C_{18}$) and UV-Vis detector (254 nm) were used. The limits of detection (LOD) and the limits of quantification (LOQ) for vitamin D were estimated to be $1.51{\mu}g/kg$ and $4.95{\mu}g/kg$, respectively. The linearity, recovery, precision and accuracy of the analytical method for vitamin D were evaluated through the application of a SRM (Standard Reference Material) 1846 (National Institute of Standard & Technology, USA). The linearity of this method was calculated with a value of the coefficient of determination ($r^2$) ${\geq}0.9999$. The recovery of vitamin D was $85.20{\pm}3.00%$. The intra-assay precision for vitamin D was between $1.68{\pm}0.03%$ and $5.75{\pm}0.33%$, and the inter-assay precision for vitamin D ranged from $1.73{\pm}0.03%$ to $2.96{\pm}0.09%$. The intra-assay accuracy for vitamin D was between $100.03{\pm}2.77%$ and $102.01{\pm}0.59%$, and the inter-assay accuracy for vitamin D ranged from $99.00{\pm}1.53%$ to $102.01{\pm}3.04%$. The proposed method is optimal for the separation and quantification of vitamin D from infant formula.

• Journal of Ginseng Research
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• 제40권4호
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• pp.415-422
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• 2016

Background: Rare ginsenosides in Panax quinquefolius L. have strong bioactivities. The fact that it is hard to obtain large amounts of rare ginsenosides seriously restricts further research on these compounds. An easy, fast, and efficient method to obtain different kinds of rare ginsenosides simultaneously and to quantify each one precisely is urgently needed. Methods: Microwave-assisted extraction (MAE) was used to extract nine kinds of rare ginsenosides from P. quinquefolius L. In this article, rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, 20(S)-Rg3, 20(R)-Rg3, Rk1, and Rg5] were identified by high performance liquid chromatography (HPLC)-electrospray ionization-mass spectrometry. The quantity information of rare ginsenosides was analyzed by HPLC-UV at 203 nm. Results: The optimal conditions for MAE were using water as solvent with the material ratio of 1:40 (w/v) at a temperature of $145^{\circ}C$, and extracting for 15 min under microwave power of 1,600 W. Seven kinds of rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, Rk1, and Rg5] had high extraction yields, but those of 20(S)-Rg3 and 20(R)-Rg3 were lower. Compared with the conventional method, the extraction yields of the nine rare ginsenosides were significantly increased. Conclusion: The results indicate that rare ginsenosides can be extracted effectively by MAE from P. quinquefolius L. in a short time. Microwave radiation plays an important role in MAE. The probable generation process of rare ginsenosides is also discussed in the article. It will be meaningful for further investigation or application of rare ginsenosides.

• 분석과학
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• 제22권6호
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• pp.514-518
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• 2009

코엔자임 큐텐은 비타민과 유사한 물질로 체내 중 미토콘드리아에 주로 분포 되어 있으며 항산화 작용을 나타낸다고 알려져 있다. 본 연구는 27-44세의 건강한 성인 남 녀 24명을 대상으로 하였고 혈장내 코엔자임 큐텐의 농도를 정량 평가하기 위하여 UV 검출기가 장착된 고성능액체 크로마토그라피를 사용 하였다. 혈장 내 코엔자임 큐텐을 분리하기 위해서 메탄올로 지방단백질을 제거하였고 노말헥산을 이용하여 액체-액체 추출법으로 코엔자임 큐텐을 용해 시켰다. 그 후 원심분리를 실시하여 노말헥산과 제단백된 물질을 층분리 시키고 상층액을 다른 유리 시험관에 옮겨 담았다. 옮겨진 노말헥산 층은 질소 가스를 이용하여 증발 농축을 시켰으며 남은 잔사에 순수한 에탄올로 재용해 시켜 검사 장비 내로 주입 하였다. 이 때 사용된 컬럼은 C18 역상 컬럼이었고 275 nm의 파장이 이용되었으며 메탄올과 에탄올을 85:15로 섞은 이동상을 1.7 mL/min의 유속으로 흘렸다. 본 검사 방법의 정량한계(limit of quantitation : LOQ, N/S=10)는 0.02 mg/L로 평가 되었고 0.1-2.0 mg/L의 농도 범위에서 검량선을 작성 하였다. 대상군 24명의 혈장 중 코엔자임 큐텐의 농도는 0.41-0.98 mg/L의 범위에서 분포 하고 있었으며 평균 농도는 $0.62{\pm}0.13mg/L$ 였다. 본 연구의 결과 본 검사 방법은 특수성을 가지고 있었으며 혈장 내 $CoQ_{10}$을 분석하는데 충분한 정량한계를 가지고 있었기에 임상에 적용이 가능하고 연구 기관에서 사용하기에 적합하다고 사료 된다.

• 공업화학
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• 제21권3호
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• pp.306-310
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• 2010

석유제품의 분별 및 유종의 불법혼입방지를 위해 현재 석유제품 내에 일정한 색을 띄는 가시적 식별제(착색제)를 혼입, 유통시키고 있다. 하지만 타유종이 미량 혼입되었을 경우, 육안으로 이를 판별하기 힘들다. 본 연구는 국내에서 사용되고 있는 가시적 식별제를 UV/Vis spectrophotometer를 이용하여, 이들을 분석할 수 있는 두 파장(370 nm, 645 nm)을 찾아내었다. 이렇게 분석된 파장에서 HPLC를 이용해 국내 석유제품 내의 가시적 식별제를 분리, 분석하였다. 또한 정상 석유제품에 타유종을 혼합한 뒤, 가시적 식별제를 분석함으로 석유제품의 불법 혼합판별을 할 수 있는 분석방법을 찾아내었다.

• Biomolecules & Therapeutics
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• 제15권2호
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• pp.123-126
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• 2007

A sensitive, simple and highly selective liquid chromatography method of determination for extraction of pseudoephedrine hydrochloride from Allegra D tablet was developed. The chief benefit of the present method is the minimal sample preparation, as the procedure is only filtering through pore syringe filter. Two drugs (pseudoephedrine hydrochloride, fexofenadine) were separated on a C$_{18}$ column and analyzed by high performance liquid chromatography (HPLC). The method had a chromatographic run time of 8.0 min. 1 ml of pseudoephedrine hydrochloride solution (1 mg/ml) was filtered through 0.22 um pore syringe filter. 50 ul of filtering solution was injected to HPLC pump and we knew the retention time (1.85 min) of separating of pseudoephedrine hydrochloride using UV detector at 280 nm. We used C$_{18}$ column (4.6 mm${\times}$250 mm), mobile phase solution (<0.05 mol/L NaH$_2$PO$_4$, 2 ml/L H$_3$PO$_4$>/CH$_3$CN / sodium dodesyl sulfate = 60 ml / 40 ml / 1 g). We separated psedoephedrine hydrochloride at run time of 1.85 min from Allegra D tablet solution (1 mg/ml) filtered through 0.22 um pore syringe filter using UV detector at 280 nm. Flow rate was set at 1.0 ml/min and the column temperature was set at 40$^{\circ}C$. Psedoephedrine hydrochloride solution (1 mg/ml) separated from Allegra D tablet was filtered through 0.22 um pore syringe filter and injected 50 ul. We confirmed the peak of psedoephedrine hydrochloride at same retention time and the separating solution was freeze-dried. In conclusion, A simple isocratic reverse-phase HPLC method has been developed that provides excellent separation of pseudoephedrine from Allegra D tablet.

• Food Science and Biotechnology
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• 제14권5호
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• pp.672-675
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• 2005

Two anthocyanins were isolated from 1% HCl-20% methanol extracts of KG 97287 black common bean (Phaseolus vulgaris L.) using semipreparative, high-performance liquid chromatography (HPLC). The anthocyanins were identified using a combination of LC/ES-mass spectrometry (MS) and spectroscopic methods of UV-Vis, $^1H-$ and $^{13}C-$ nuclear magnetic resonance (NMR). The chemical structures of these two anthocyanins were elucidated as delphinidin 3-glucoside and petunidin 3-glucoside and their contents in KG 97287 black common bean seed coats were determined to be $2.614{\pm}0.11$ and $0.167{\pm}0.01\;mg/g$, respectively. These contents were lower than reported internationally and we recommend the introduction into Korea of high anthocyanin varieties of black common bean.

• Journal of Applied Biological Chemistry
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• 제62권1호
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• pp.87-91
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• 2019

The contents of major ginsenosides (ginsenosides Rb1, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf, and ginsenoside Rg1) in ginseng cultivated in different areas in Korea, ginseng that underwent different cultivation processes and ages, and ginseng cultivated in different countries were determined using high-performance liquid chromatography equipped with UV/VIS detector. Ginsenoside Rc was the most abundant ginsenoside in all different ginseng samples. The highest total concentration of major ginsenosides was found in the ginseng cultivated in Jinan (0.931 mg/g) and 4-year grown red ginseng (1.785 mg/g). Major ginsenosides were the most abundant in Korean ginseng (1.264 mg/g), compared to those in Chinese and American ginseng. The results of this study showed the different contents of major ginsenosides in the ginseng samples tested and emphasized which sample could contain high yield of ginsenosides.

• 한국재료학회지
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• 제33권11호
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• pp.458-464
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• 2023

In order to develop catechin patches for skin regeneration at wound sites, patches with varying concentrations of catechin and chitosan were manufactured. An optimal composition ratio was determined by adjusting the drug release rate and amount, to maximize efficiency. The catechin used in this study was extracted from green tea leaves using a solvent/ultrasonication method, and its characteristics were confirmed through Fourier transform-infrared spectroscopy (FT-IR) and high-performance liquid chromatography (HPLC) analyses. Patches were prepared with different concentrations of catechin and chitosan, and various properties were analyzed using techniques such as FT-IR, water contact angle analysis, and UV-Vis spectroscopy. It was observed that as the chitosan concentration increased, the release of catechin slowed down or almost ceased. A patch manufactured with 1.5 mg/cm² of catechin at a 1 % chitosan concentration exhibited a high initial release rate over 24 h and demonstrated cellular biocompatibility. Consequently, these patches, with tailored release characteristics based on the concentrations of chitosan and catechin, hold promise for use as drug delivery systems in wound healing applications.