• 한국식품영양과학회지
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• 제19권1호
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• pp.1-12
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• 1990

To investigate effects of ethanol and dietary fat on growth and bichemical indices of liver tissue and blood in rats 40 male rats of Sprague-Dawley wtrain weighing about 160g were divided into 5 groups (low-fat diet group ethanol-administered low-fat diet group high-fat diet group ethanol-administered high-fat diet group and commercial diet group) and fed expe-rimental diets for 8 weeks. Ethanol-administered groups consumed ethanol corresponding to 22 cal% which was considered as moderate drinking. Neither the ethanol intake nor the dietary fat level affected calorie intake. Nonetheless the low-fat diet group with ethanol had the lowest growth rate and 2-fold increase in the concentration of plasma triglyceride. There was no effect of ethanol and dietary fat level on contents of protein lipid and lipid composition of liver tissue. The level of lipid peroxide of liver tissue tended to be increased by ethanol intake but the increase was statistically insignificatnt. The low-fat ethanol group had lowered hepatic mitochondrial respiration rate and deformed structure of mitochondria of hepatocytes.

• 동아시아식생활학회지
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• 제25권4호
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• pp.607-615
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• 2015

Our final goal was to manufacture low-calorie healthy seafood spreads using high pressure processing. Prior to developing the spread products, we performed a nationwide survey of consumers aged in their teens and twenties (n=585). The questionnaire was designed to gain insights from consumers on spread products, including Importance-Performance Analysis (IPA), recipes, market prospects, etc. Consumers responded that 'price, calorie, fat content, sanitary, nutrition, and food additive' should be improved, in that order. They also thought that fruits and vegetables are highly acceptable in recipes of seafood spreads. In addition, consumers pointed that we should concentrate on 'taste, sanitary, flavor, nutrition, and price' to make spreads more successful. Regarding market prospects, consumers rated spreads with scores over 2.96/5.00, which means the market for seafood spreads is sufficient. The consumers' response provided guidelines for developing and manufacturing low-calorie healthy seafood spreads.

• 한방비만학회지
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• 제21권2호
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• pp.59-68
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• 2021

Objectives: We investigated the effects of Clean-DM4 (C-DM4), a polyherbal extract consisting of Coptidis Rhizoma, Salviae Miltiorrhiza Radix, and Cinnamomi Cortex on high fat diet (HFD)-induced obesity and diabetes in mice. Methods: The C57BL/6 mice (6 weeks) were fed a HFD for 8 weeks and then administrated with C-DM4 extract at 500 mg/kg (p.o.) once daily for 4 weeks. The changes of body weights, calorie intakes, and fasting blood glucose (FBG) levels were measured in mice. The serum levels of glucose, insulin, total cholesterol, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were measured in mice by enzyme-based assay. It was also observed the histological changes of pancreas, liver, and fat tissues with hematoxylin and eosin (H&E) staining. Results: The increase of calorie intakes and FBG levels in HFD-induced obesity mice was significantly decreased by oral administration of C-DM4 extract. C-DM4 extract administration was significantly reduced the increased levels of glucose, insulin, total cholesterol, AST, and ALT in obesity mice. In addition, C-DM4 extract inhibited lipid droplet accumulation in liver tissues of obesity mice, hyperplasia of pancreatic islets, and enlargement of adipocytes in adipose tissues. Conclusions: Our study indicates that C-DM4 extract could help improve obesity and to prevent diabetes progression.

• Journal of Nutrition and Health
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• 제40권2호
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• pp.118-129
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• 2007

This study was performed to investigate the effect of lysine-limited diets containing different levels of L-carnitine on body weight and lipid metabolism in obesity-induced adult rats. Eight-month-old male Sprague-Dawley rats (n = 90) were raised for one month with high fat diet (40% fat as calorie) to induce obesity. After induction of obesity, rats weighing 739.5 g were randomly blocked into three groups according to the body weight and raised for eight weeks with control diet (Co), 50% lysine-limited diet (-L), 50% lysine limitation with 0.3% pivalate diet (-L + P). Each of three groups was allotted to 0.0% L-carnitine (0.0% CT), 0.5% L-carnitine (0.5% CT) and 2.5% L-carnitine (2.5% CT) groups, respectively. The levels of AST, ALT, total protein and albumin in plasma were within the normal range. Daily food intake and calorie intake tended to be lower in 2.5% CT groups than those of other groups regardless lysine limitation or pivalate intake. And body weight gain and calorie efficiency ratio (weight gain (g) /calorie intake (100 kcal)) were significantly the lowest in 2.5% CT groups among all experimental groups regardless of lysine limitation or pivalate intake. The weights of perirenal, epididymal fat pads and brown adipose tissue in 2.5% CT groups were significantly lower than 0.0% CT groups. Plasma total lipid, triglyceride, total cholesterol concentrations in all groups were not significant by experimental compound. HDL-cholesterol concentrations in -L + P +2.5% CT group were highest in -L + P groups. Levels of hepatic total lipid, triglyceride and total cholesterol in 2.5% CT groups were tend to be lower those than in 0.0% CT groups regardless of dietary lysine limitation and pivalate intake. Fecal total lipid excretions of 2.5% CT groups were significantly lower than in 0.0% CT groups in all experimental groups. But fecal triglyceride excretions of 2.5% CT groups were significantly higher than 0.0% CT groups regardless of lysine limitation and pivalate. In conclusion, there was no difference on body weight and lipid metabolism by dietary lysine limitation and pivalate intake. And feeding of 2.5% L-carnitine was more effective than feeding of 0.5% L-carnitine and 0.0% L-carnitine in reduction of body weight, body fat and lipid metabolism.

• Journal of Nutrition and Health
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• 제21권3호
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• pp.154-163
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• 1988

Effects of alchohol and fat content in a balanced diet on chemical composition and morphology of liver were investigated in growing rats. Fourth eight male rats of Sprague-Dawley strain weighing about 160g were divided into 4 groups ; high fat diet group, alcohol-administered high fat diet group, low fat diet group and alcohol-administered high fat diet group, low fat diet group and alcohol-administered low fat diet group. High and low fat diets supplied 30% and 12%, respectively, of total calorie intake from fat, and alcohol was given by adding ethanol in drinking waster at 10%. Diets contained adequate amounts of all nutrients required for rats, including lipotrpoic agents(choline and methionine) to minimize effects of factors other than alcohol on liver damage. Ratios of liver weight to body weight were statistically different among groups. Liver/dody weight ratios alcohol-administered rats were significantly higher than those of non-alcohol groups after 6 weeks treatment. Although total lipid and triglyceride per gram liver were increased in alcohol-administered rats, especially low fat diet fed rats, the values were not significantly different. Opticmicroscopical observation revealed increase in cell size and no change in morphology of liver. Examination of hepatocytes by electron microscopy showed that fat droplets were observed in all groups but enlarged in the alcohol-administered low fat diet fed rat. Contents of protein, cholesterol and phospholipid were not affected by alcohol consumption. The level of lipid peroxide was significantly lower in the livers of alcohol-administered rats than in the livers of non-alcohol groups. The results of this study indicate that even moderate alcohol drinking and dietary fat content did not affect any significant change in composition and morphology of liver until 6 week treatment but that even moderate alcohol drinking caused some signs of steatosis of liver.

• 한국식생활문화학회지
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• 제1권2호
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• pp.157-162
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• 1986

This study was conducted to research on the nutritional status of vegetarian boys high school students, to keep abreast with the international trend of stressing the vegetarian diet, thereby analyzing the degree of the influence of their dietary habit on their growth. The object of the study were 62boys high school students who are lacto-ovo vegetarians, and the author adopted three popular research methods. questionaire physical test and blood test Though vegetarian diet is helpful for the prevention of coronary and heart diesedse of the adults, but it is not enough for the students who are needed lot of protein and calorie.

• Nutrition Research and Practice
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• 제11권2호
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• pp.105-113
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• 2017

BACKGROUND/OBJECTIVES: A high-fat diet (HFD) induces obesity, which is a major risk factor for cardiovascular disease and cancer, while a calorie-restricted diet can extend life span by reducing the risk of these diseases. It is known that health effects of diet are partially conveyed through epigenetic mechanism including DNA methylation. In this study, we investigated the genome-wide hepatic DNA methylation to identify the epigenetic effects of HFD-induced obesity. MATERIALS AND METHODS: Seven-week-old male C57BL/6 mice were fed control diet (CD), calorie-restricted control diet (CRCD), or HFD for 16 weeks (after one week of acclimation to the control diet). Food intake, body weight, and liver weight were measured. Hepatic triacylglycerol and cholesterol levels were determined using enzymatic colorimetric methods. Changes in genome-wide DNA methylation were determined by a DNA methylation microarray method combined with methylated DNA immunoprecipitation. The level of transcription of individual genes was measured by real-time PCR. RESULTS: The DNA methylation statuses of genes in biological networks related to lipid metabolism and hepatic steatosis were influenced by HFD-induced obesity. In HFD group, a proinflammatory Casp1 (Caspase 1) gene had hypomethylated CpG sites at the 1.5-kb upstream region of its transcription start site (TSS), and its mRNA level was higher compared with that in CD group. Additionally, an energy metabolism-associated gene Ndufb9 (NADH dehydrogenase 1 beta subcomplex 9) in HFD group had hypermethylated CpG sites at the 2.6-kb downstream region of its TSS, and its mRNA level was lower compared with that in CRCD group. CONCLUSIONS: HFD alters DNA methylation profiles in genes associated with liver lipid metabolism and hepatic steatosis. The methylation statuses of Casp1 and Ndufb9 were particularly influenced by the HFD. The expression of these genes in HFD differed significantly compared with CD and CRCD, respectively, suggesting that the expressions of Casp1 and Ndufb9 in liver were regulated by their methylation statuses.

• 한국식품영양과학회지
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• 제41권8호
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• pp.1094-1099
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• 2012

본 연구는 실험쥐 20마리를 사용하여 5주간의 고지방식이를 섭취시킨 후 CON군과 HF군으로 구분하여 식이조성에 따른 체중과 체구성 그리고 24시간 동안의 에너지 대사를 분석하였다. 그 결과 고지방식이로 인한 체중의 유의한 차이는 없었다. 5주간의 식이섭취량은 두 군간 유의한 차이가 있었고, 섭취열량은 약 6% 정도의 유의한 차이가 있었다. 산소섭취량(에너지소비량)은 두 군 간의 유의한 차이가 없었으나, 이산화탄소배출량은 유의한 차이를 보였다. 고지방식이군은 24시간 동안 유의하게 높은 지방산화량을 보였고 일반식이군은 탄수화물산화량이 유의하게 더 높은 것으로 나타났다. 특히 활동기에 두 군의 에너지 이용 기질에 많은 변화가 있는 것으로 확인되었다. 두 군간 소비에너지는 유의한 차이가 없었으나 섭취에너지에 비해 HF군의 복강 내 지방이 약 33% 이상 유의하게 높게 나타났다. 이상의 연구결과를 종합하여 볼 때 식이구성에 의하여 에너지이용기질이 달라지는 것을 확인할 수 있었다. 또한 5주간 섭취한 열량과 소비된 에너지 비를 비교하였을 때 고지방식이군에서 복강내 지방이 유의하게 증가한 것은 체내에서의 지방의 식이효율이 그동안 알려진 Atwater의 값(9 kcal/g)보다 더 높은 것으로 사료되어진다. 이는 탄수화물과 고지방식이섭취에서 체내에너지 저장량을 분석하였을 때 탄수화물의 열량은 4 kcal/g인데 비해 지방은 약 11.1 kcal/g으로 보고한 기존의 연구보고(21)를 지지하는 결과로 나타났다. 이러한 결과는 일반인 및 비만환자와 대사증후군 환자의 체중조절과 개선에 있어서 식이구성과 일일영양권장량의 재검토의 필요성을 시사하고 있으며 또한 전문운동선수들의 체급별 체중조절에 필요한 식단의 재구성 등 스포츠현장의 기초자료가 될 것으로 판단되어진다. 그러나 본 연구는 대사과정에 관여하는 여러 호르몬과 단백질 등의 분석이 이뤄지지 않았고, 에너지대사량과 조직의 중량만을 검토한 결과이므로 추후 체내에서의 지방산화와 관련된 호르몬 및 단백질 등의 측정과 전체 피하조직 및 근육 등에 저장된 지방의 분석을 포함한 추가연구가 필요한 것으로 보인다.

• 한국식생활문화학회지
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• 제9권3호
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• pp.277-288
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• 1994

In recent years, there is remarkable tendency of overconsumption and high calorie intake which may come from a high availability of foods, the increase of food production, processed food, and imported food. This cause chronic diseases such as obesity. high blood pressure inducing heart disease, and diabetics etc. Traditional Korean eating pattern and habit make too much waste of foods and wasting time for the food preparation. The dietary guideline used for the guidance of Korean diet pattern is composed of 'nutrients-ingredient-cooking-meal which is not visualized pattern. Therefore we made up menus in the pattern of meal-cooking-ingredient-nutrient, which is more brief, compact and more visualized pattern. We set 27,000 menus with 600 kcal/meal which is combined with staple meal-main dish-side dish (30 menus of rife meal, 5 menus of bread meal, noodle meal and one serving dish meal). 600 kcal menu is basically for volume of serving per one person according to the experimental cooking and reference. This has several advantages of simple procedure of cooking, easy practice to use menus, and good source of nutritional values. In addition to these, it also provides a new menu to decrease the calorie consumption reducing the risk of chronic disease, to prevent wasting foods, and to help single person.

• 운동영양학회지
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• 제15권4호
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• pp.163-171
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• 2011

The effects of a high-fat diet and fasting on resting energy expenditure and energy substrate utilization were examined using the method of measuring whole body energy metabolism and oxygen uptake. Eight 4-week old male Sprague-Dawley rats were used for the high-fat diet experiment. Energy metabolism was measured using acrylic metabolic chambers over 24 hours. After 1-week of preliminary feeding, 4 rats were fed a chow diet, whereas the remaining 4 rats were fed a high-fat diet (HF) ad libitum, which contained 40% (w/w, calorie base 60%) more fat than that in the chow diet. The flow rate to measure energy metabolism inside the chamber was controlled at a mean of 3.5 L/min, and five chambers were subjected to measurement. One of the five chambers was used to correct errors by measuring the atmosphere. As a result of 5 weeks of control diet and high-fat diet feeding, body weight of the high-fat diet group tended to increase more than that in the control diet fed group, but the difference was not significant. Oxygen uptake and carbon dioxide production changed as time went on over the 24 hr. The respiratory exchange ratio also changed during the 24 hr, and the difference between the groups was significant. The control group showed significantly more carbohydrate oxidation than that of the high-fat diet fed group. A fasting experiment was conducted using six 7-week old Sprague-Dawley male rats. Energy metabolism measurements were performed using the same method as that used in the high-fat diet experiment; resting metabolism was measured prior to fasting, and a fasting condition began from 9:00 am the next day for 3 days to calculate energy metabolism. Both body weight and 24-hour oxygen uptake decreased significantly as a result of 3-day fasting. Total oxygen uptake in the first day decreased, and declined significantly on day 3 of fasting. Total 24-hour carbon dioxide production decreased significantly over the 3 days. The mean 24-hour respiratory exchange ratio decreased significantly. Additionally, energy expenditure during the dark period (20:00-08:00), which is the active period for rats, decreased significantly with fasting, whereas energy expenditure during the light period (08:00-20:00) did not increase by fasting.