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Effect of Lysine-Limited Diets Containing Different Levels of L-Carnitine on Body Weight and Lipid Metabolism in Obesity-Induced Adult Rats  

Kim, Ja-Kyung (Department of Food & Nutritional Sciences, Ewha Womans University)
Kim, Mi-Kyung (Department of Food & Nutritional Sciences, Ewha Womans University)
Publication Information
Journal of Nutrition and Health / v.40, no.2, 2007 , pp. 118-129 More about this Journal
Abstract
This study was performed to investigate the effect of lysine-limited diets containing different levels of L-carnitine on body weight and lipid metabolism in obesity-induced adult rats. Eight-month-old male Sprague-Dawley rats (n = 90) were raised for one month with high fat diet (40% fat as calorie) to induce obesity. After induction of obesity, rats weighing 739.5 g were randomly blocked into three groups according to the body weight and raised for eight weeks with control diet (Co), 50% lysine-limited diet (-L), 50% lysine limitation with 0.3% pivalate diet (-L + P). Each of three groups was allotted to 0.0% L-carnitine (0.0% CT), 0.5% L-carnitine (0.5% CT) and 2.5% L-carnitine (2.5% CT) groups, respectively. The levels of AST, ALT, total protein and albumin in plasma were within the normal range. Daily food intake and calorie intake tended to be lower in 2.5% CT groups than those of other groups regardless lysine limitation or pivalate intake. And body weight gain and calorie efficiency ratio (weight gain (g) /calorie intake (100 kcal)) were significantly the lowest in 2.5% CT groups among all experimental groups regardless of lysine limitation or pivalate intake. The weights of perirenal, epididymal fat pads and brown adipose tissue in 2.5% CT groups were significantly lower than 0.0% CT groups. Plasma total lipid, triglyceride, total cholesterol concentrations in all groups were not significant by experimental compound. HDL-cholesterol concentrations in -L + P +2.5% CT group were highest in -L + P groups. Levels of hepatic total lipid, triglyceride and total cholesterol in 2.5% CT groups were tend to be lower those than in 0.0% CT groups regardless of dietary lysine limitation and pivalate intake. Fecal total lipid excretions of 2.5% CT groups were significantly lower than in 0.0% CT groups in all experimental groups. But fecal triglyceride excretions of 2.5% CT groups were significantly higher than 0.0% CT groups regardless of lysine limitation and pivalate. In conclusion, there was no difference on body weight and lipid metabolism by dietary lysine limitation and pivalate intake. And feeding of 2.5% L-carnitine was more effective than feeding of 0.5% L-carnitine and 0.0% L-carnitine in reduction of body weight, body fat and lipid metabolism.
Keywords
L-carnitine; lysine-limitation; pivalate; body weight;
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1 Jogl G, Hsiao YS, Tong L. Structure and function of carnitine acyltransferases. Ann N Y Acad Sci 1033: 17-29, 2004   DOI
2 Fujii R, Chiba S, Numazaki K, et al. Effect of cefditoren pivoxil on carnitine metabolism in pediatric patients. Jpn J Antibiot 46 (10): 926-937, 1993
3 Park JY, Kim MK. Effect of feeding garcinia cambogia extract (HCA) and/or L-carnitine and exercise on body weight in rats. Korean Nutrition Soc 38(8): 637-648, 2005   과학기술학회마을
4 Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911-917, 1959   DOI
5 Korean Society of Food Science and Nutrition. Handbook of experiments in food science and nutrition-Nutrition, pp.655-676, Hyoil, 2000
6 Clouet P, Sempore G, Tsoko M. Effect of short- and long-term treatments by a low level of dietary L-carnitine on parameters related to fatty acid oxidation in Wistar rat. Biochim Biophys Acta 1299(2): 191-197, 1996   DOI
7 Madore F. The clinical epidemiology of cardiovascular diseases in chronic kidney disease. Semin Dial 16: 148-156, 2003   DOI   ScienceOn
8 Argani H, Rahbaninouber M, Ghorbanihagjo A, Golmohammadi Z, Rashtchizadeh N. Effect of L-carnitine on the serum lipoproteins and HDL-C subclasses in hemodialysis patients. Nephron Clin Pract 101: C174-C179, 2005   DOI   ScienceOn
9 Elisaf M, Bairaktari E, Katapodis K, Pappas M, Sferopoulos G, Tzallas C, et al. Effect of L-carnitine supplementation on lipid parameters in hemodialysis patients. Am J Nephrol 18: 416-421, 1998   DOI   ScienceOn
10 Gloggler A, Bulla M, et al. Effect of low dose supplementation of L-carnitine on lipid metabolism in hemodialyzed children. Kidney Int Suppl 27: S256-S258, 1989
11 Seccombe DW, James L, Hahn P, Jones E. L-carnitine treatment in the hyperlipidemic rabbit. Metab 36: 1192-1196, 1987   DOI   ScienceOn
12 Yoon HR, Hong YM, Boriack RL, Bennett MJ. Effect of L-carnitine supplementation on cardiac carnitine palmitoyltransferase activities and plasma carnitine concentrations in adriamycin-treated rats. Pediatr Res 53: 788-792, 2003   DOI   ScienceOn
13 Platell C, Kong SE, McCauley R, Hall JC. Branched-chain amino acids. J Gastroenterol Hepatol 15: 706-717, 2000   DOI   ScienceOn
14 Frings CS, Dunn RT. A colorimetric method for determination of total serum lipids based on the sulforic-phospho-vanillin reaction. Am J Clin Nutr 53(1): 89-91, 1970
15 Corrina B, Klaus E. Effect of L-carnitine on weight loss and body composition of rats fed a hypocaloric diet. Ann Nutr Metab 46: 205-210, 2002   DOI   ScienceOn
16 Mokady S, Einav P. Effect of dietary wheat gluten on lipid metabolism in growing rats. Nutr Metab 22: 181-190, 1978   DOI
17 Melegh B, Kerner J, Beiber L. Pivampicillin-promoted excretion of pivaloylcarnitine in humans. Biochem Pharmacol 36: 3405- 3409, 1987   DOI   ScienceOn
18 Lee HS, So YH. The Effects of Diet Effect on Diet Practice Extent in College Students. J Sports Leisure Studies 25: 419-429, 2005
19 Cederblad G, Holm J, Lindstedt G, Lindstedt S, Nordis I, Schersten T. Gamma-Butyrobetaine hydroxylase activity in human and ovine liver and skeletal muscle tissue. FEBS Letters 98(1): 57-66, 1979   DOI   ScienceOn
20 Fujii R, Sunakawa K, et al. Safety and carnitine status after oral administration of S-1108 to pediatric patients. Chemotherapy 41: 655-665, 1993
21 Ikeda K, Cha JY, Yanagita T, Makatani N, Oogami K, Imaizumi K, Yazawa K. Effects of dietary ${\alpha}$-linolenic, eicosapentaenoic and dicisahexaenoic acids on hepatic lipogenesis and ${\beta}$-oxidation in rats. Biosci Biotechnol Biochem 62(4): 675-680, 1998   DOI   ScienceOn
22 Holme E, Greter J, Jacobson CE, et al. Carnitine deficiency induced by pivampicillin and pivmecillinam therapy. Lancet 2: 469- 473, 1989
23 Yasikazu T, Susaki R, Fukui F, Waki H, et al. Acetyl-L-carnitine supplementation restores decreased tissue carnitine levels and impaired lipid metabolism in aged rats. J Lipid Res 45: 729-735, 2004   DOI   ScienceOn
24 Nakajima H, Kodo N, Inoue F, Kizaki Z, Nukina S, et al. Pivalate affects carnitine status but causes no severe metabolic changes in rat liver. J Nutr 126: 1683-1687, 1996   DOI
25 Tanphaichitr V, Broquist HP. The Journal of Biological Chemistry. Volume 248, 1973: Role of lysine and epsilon-n-trimethyllysine in carnitine biosynthesis II. Studies in the rat. Nutr Rev 46(4): 164-166, 1988   DOI   ScienceOn
26 Alison S, Janos K, Charles LH. Carnitine: a nutritional, biosynthetic and functional perspective. Mol Asp Med 25: 455-473, 2004   DOI   ScienceOn
27 Raymond TL, Reynolds SA, Swanson JA, Patnode CA, Bell FP. The effect of oral L-carnitine on lipoprotein in the Watanabe heritable hyperlipidemic rabbit (Oyctolagus cuniculus). Comp Biochem Physiol 88A: 503-506, 1987
28 Haigler HT, Broquist HP. Carnitine synthesis in rat tissue slices. Biochem Biophys Res Commun 56(3): 676-681, 1974   DOI   ScienceOn
29 Feng Y, Guo C, Wei J, Yang J, Ge Y, Gao L. Necessity of carnitine supplementation in semistarved rats a high-fat diet. Nutrition 17: 628-631, 2001   DOI   ScienceOn
30 Rahman SM, Wang YM, Yostumoto H, Cha JY, Han JY, Inoue S, Yanagita T. Effects of conjugated linolenic acid on serum leptin concentration, body-fat accumulation, and ${\beta}-oxidation$ of fatty acid in oletf rats. Nutrition 17: 385-390, 2001   DOI   ScienceOn
31 Janos K, Charles H. Fatty acid import into mitochondria. Biochem Biophys Acta 1486: 1-17, 2000
32 Kim HT, Lee H, Kim WS, Kim DJ, Ahn ES, Ahn EN, Son TY. Exercise and L-carnitine. Korean J Exer Nutr 5(1): 1-12, 2001
33 Muller DM, Seim H, Kiess W, Loster H, Richer T. Effects of oral L-carnitine supplementation on in vivo long-chain fatty acid oxidation in health adults. Metab 51(11): 1389-1391, 2002   DOI   ScienceOn
34 Ministry of Health and Welfare. 2001 National health and nutrition survey, overview, 2002
35 Maebashi M, Imamura A, Yoshinaga K. Effect of aging on lipid and carnitine metabolism. J Exp Med 138: 231-236, 1982
36 Marccarri F, Pessotto N, Ramacci MT, Angelucci L. The effect of exogenous L-carnitine on fat diet-induced hyperlipidemia in the rat. Life Sci 36: 1967-1975, 1985   DOI   ScienceOn
37 Siram K, Pinchrofsky G, Kaminski MV. Suppression of appetite by parenteral nutrition in humans. J Am Coll Nutr 3: 317-323, 1984   DOI
38 Villani RG, Gannon J, et al. L-carnitine supplementation combined with aerobic training dose not promote weight loss in moderately obese women. Int J Sport Nutr Exerc Metab 10: 199-207, 2000   DOI
39 Ministry of Health and Welfare. 2005 National health and nutrition survey, overview, 2006
40 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin phenol reagent. J Biol Chem 193: 265-275, 1951
41 Chung EJ, Um YS, Cha YS, Park TS. Effects of short-term supplementation of carnitine on plasma and urinary carnitine and plasma lipid levels of health male adults. Korean Nurs Soc 36(7): 720- 728, 2003
42 Broderick TL, Christos S, Wolf BA, DiDomenico D, Shug A, Paulson D. Fatty acid oxidation and cardiac function in the sodium pivalate model of secondary carnitine deficiency. Metab 44: 499-505, 1995   DOI   ScienceOn
43 Rauchova H, Dobesova Z, Drahota Z, Zicha J, Kunes J. The effect of chronic L-carnitine treatment on blood pressure and plasma lipids in spontaneously hypertensive rats. Eur J Pharmacol 342: 235-239, 1998   DOI
44 Maccari F, Arseni A, Chiodi P, Ramacci T, Angelucci L. Levels of carnitines in brain and other tissues of rats of different ages: effect of acetyl-L-carnitine administration. Exp Gerontol 25: 127- 134, 1990   DOI   ScienceOn
45 Aoyama Y, Kondo H, Ashida K. Lipid accumulation in the liver of rats fed a lysine-deficient diet. Nutr Rep Int 14: 641-655, 1976
46 Alessandro L, Michael MM, Tad R, Zhong JY, Lee J. Beverly. Carnitine supplementation accelerates normalization of food intake depressed during TPN. Physiol Behav 60(1): 317-320, 1996   DOI   ScienceOn
47 Matera M, Bellinghier G, Costantino G, Santoro D, Calvani M, Savica V. History of L-carnitine; implications for renal disease. J Ren Nutr 13: 2-14, 2003   DOI   ScienceOn
48 Borum PR, Broquist HD. Lysine deficiency and carnitine in male and female rats. J Nutr 107: 1209-1215, 1977   DOI
49 Bianchi PB, Davis AT. Sodium pivalate treatment reduces tissue carnitine and enhances ketosis in rats. J Nutr 121: 2029-2036, 1991   DOI
50 Horne DW, Tanphaichitr V, Broquist HP. Role of lysine in carnitine biosynthesis in Neurospora crassa. J Biol Chem 246: 4373- 4375, 1971