• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.67 no.4
• /
• pp.265-273
• /
• 2022

Waxy corn (Zea mays L.), which contains homozygous mutant alleles for the waxy1 (wx1) gene, is widely consumed as a snack food in Asia. This study evaluated sixteen agronomic characteristics of inbred Korean waxy corn lines to aid development of high-quality waxy corn cultivars. The plant materials studied were 177 inbred waxy corn lines developed by the National Institute of Crop Science, Rural Development Administration, Republic of Korea. For the tested lines, days to tasseling and silking averaged 77.69±2.22 days (with a range of 56-97 days), and 81.12±7.56 days (66-99 days), respectively. Plant length ranged from 88 to 237 cm (averaged 164.88±22.67 cm), ear length averaged 11.75±2.52 cm (5.0-18.5 cm), and ear width averaged 2.94±0.68 cm (1.4-4.5 cm). The number of rows on each ear of corn averaged 12.22±2.22 (7-32 rows) and the kernel number averaged 24.30±4.22 (9-37 kernels) per row. The crude protein content was 12.05±1.53% (8.90-21.80%) and total starch content was 69.27±5.74% (49.5-83.9%). Principal component analysis revealed that ear width, grain length, ear length, days to tasseling, days to silking, percentage of ear setting height, and total starch are features that allow distinction between the 177 waxy inbred corn lines. Hierarchical cluster analysis identified twelve waxy inbred lines that produce tall plants and have a short silking period. These lines may improve yield among quickly growing corn varieties.

• Korean Security Journal
• /
• no.51
• /
• pp.103-124
• /
• 2017

The purpose of this study is to analyze regional clusters and discrimination on size and sector of private security in national 16 areas in 2015. The results of the study using hierarchical cluster analysis and discriminant analysis are as follows. First, we can classify national 16 areas into 3 clusters, such as Large Area(1), Medium Area(2), and Small Area(3) according to the size of private security. The discriminant functions D for each cluster is as follows: (1) Large Area(1) = -383.981 + (.108 ${\times}$ number of security companies) + (.016 ${\times}$ number of guards), (2) Medium Area(2) = -35.570 + (.029 ${\times}$ number of security companies) + (.005 ${\times}$ number of guards), and (3) Small Area(3) = -5.381 + (.012 ${\times}$ number of security companies) + (.002 ${\times}$ number of guards). Second, we can classify national 16 areas into 3 clusters, such as Large Area(1), Medium Area(2), and Small Area(3) according to the sector of private security. The discriminant function D for each cluster is as follows: (1) Large Area(1) = -2224.402 + (-.562 ${\times}$ facilities security) + (1.245 ${\times}$ protection security) + (171.142 ${\times}$ convoy security) + (-2.722 ${\times}$ electronic security) + (2.020 ${\times}$ special security), (2) Medium Area(2) = -4.762 + (.052 ${\times}$ facilities security) + (.063 ${\times}$ protection security) + (-3.819 ${\times}$ convoy security) + (.110 ${\times}$ electronic security) + (-.165 ${\times}$ special security), and (3) Small Area(3) = -125.742 + (-.009 ${\times}$ facilities security) + (.432 ${\times}$ protection security) + (5.748 ${\times}$ convoy security) + (5.530 ${\times}$ electronic security) + (-.901 ${\times}$ special security).

• Korean Journal of Ecology and Environment
• /
• v.54 no.1
• /
• pp.24-38
• /
• 2021

Microhabitat In the upper stream is created by various environment variables such as the bottom substrate and the physicochemical factors, and may influence the distribution of benthic macroinvertebrates. We investigated the bottom substrate and environmental variables influencing the distribution of benthic macroinvertebrate in 26 stream-type waterways established at upper reaches of Geum River. During study period, total 85 families, 160 species, 9305 individuals of benthic macroinvertebrates were recorded. The stream-type waterways, where the bottom substrates consist mainly of pebble (16~64 mm) and cobble (64~256 mm) or with rapid water velocity (more than 0.2 m/s) and high dissolved oxygen (more than 120%), were supported by high species diversity of benthic macroinvertebrate. Hierological cluster analysis and the nonparametric multidimensional scale (NMDS) divided 26 stream-type waterways into a total of three clusters. In Cluster 1, the invertebrate species, such as Branchiura sowerbyi, Cloeon dipterum, Ischnura asiatica, Paracercion calamorum, and Radix auricularia, closely related to aquatic macrophytes, and Chironomidae spp., Limnodrilus gotoi, and Tanypodinae sp. were abundant in waterways, with high coverage of silt and clay as well as high turbidity and total nitrogen. The benthic macroinvertebrate species (Cheumatopsyche brevilineata, Drunella ishiyamana, Dugesia japonica, Ephemera orientalis, Gumaga KUa, Macrostemum radiatum, Potamanthus formosus, Semisulcospira libertine, Stenelmis vulgaris, and Teloganopsis punctisetae) included in Cluster 2 were dominated in sites with high cover rates of pebble and gravel. Cluster 3 was predominantly covered by the Cobbles, was supported by Simulium sp. Such a clear distinction in the study sites means that each stream-type waterways is governed by a clear habitat environment. In the case of some sites with low species diversity, improvement measures are required to restore nature, such as improving the function of inflows and outflows, creating meandering channel, and inducing the settlement of littoral vegetation.

• The Journal of Internal Korean Medicine
• /
• v.30 no.3
• /
• pp.594-603
• /
• 2009

Objectives : ICH breaks down blood vessels within the brain parenchyma, which finally leads to neuronal loss, drugs to treat ICH have not yet been established. In this experiment, we measured the effect of Woowhangchongshim-won (WWCSW) on intracerebral hemorrhage (ICH) in rat using microarray technology. Methods : We measured the effect of WWCSW on ICH in rat using microarray technology. ICH was induced by injection of collagenase type IV, and total RNA was isolated. Image files of microarray were measured using a ScanArray scanner, and the criteria of the threshold for up- and down-regulation was 2 fold. Hierarchical clustering was implemented using CLUSTER and TREEVIEW program, and for Ontology analysis. GOSTAT program was applied in which p-value was calculated by Chi square or Fisher's exact test based on the total array element. Results : WWCSW-treatment restored the gene expression altered by ICH-induction in brain to the levels of 76.0% and 70.1% for up- and down-regulated genes, respectively. Conclusion : Co-regulated genes by ICH model of rat could be used as molecular targets for therapeutic effects of drug including WWCSW. That is, the presence of co-regulated genes may represent the importance of these genes in ICH in the brain and the change of expression level of these co-regulated genes would also indicate the functional change of brain tissue.

• Toxicological Research
• /
• v.22 no.2
• /
• pp.95-102
• /
• 2006

Exposure to soluble nickel compound produces toxic effects on immune system, but the mechanism of action remains to be elucidated. Differential gene expression was studied to understand the potential molecular mechanism responsible for acute toxicity induced by nickel acetate in spleen cells. We exposed mouse spleen cells to nickel acetate with a nontoxic dose ($40{\mu}M$) and then extracted total RNA at 6 h and 12 h after exposure. The RNA was hybridized onto 10K mouse oligonucleotide microarrays, and data were analyzed using GeneSpring 7.1. Nickel had a modest effects on expression of many genes, in the range of 1.3-3 fold. The expression profile showed time-dependent changes in expression levels of differentially expressed genes, including some important genes related to cell cycle, apoptosis and DNA repair. In hierarchical cluster analysis of duplicate experiments, 111 genes were screened out. Out of these, 44 genes showing time- dependent up-regulation (>1.5 fold) and 38 genes showing down-regulation (>1.5 fold) at all time points were chosen for further analysis. The change in the expression of three genes (GPX1, GADD45B and FAIM) after nickel treatment was validated using RT-PCR. As a rule, a number of genes appear to be coordinately regulated between cell survival and cell death from nickel toxicity. In conclusion, changes in the gene profile in the spleen after nickel treatment are complex and genes with diverse functions are modulated. These findings will be contributed to the understanding of the complicated biological effects of nickel.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.51 no.4
• /
• pp.567-575
• /
• 2015

Species composition in the coastal waters off Ulleungdo of Korea were examined based on catches bimonthly collected by trammel net in 2013. A total of 711 individuals and 181.9 Kg were caught and catches were composed of 4 classes 15 orders 27 families 52 species including 44 Pisces, 4 Gastropoda, 3 Cephalopoda, and 1 Echinodermata. The dominant species in biomass were File fish (Thamnaconus modestus), Atka mackerel (Pleurogrammus azonus), and Greenling (Hexagrammos otakii). Data were summarized using hierarchical cluster analysis (HCA) and detrended correspondence analysis (DCA) to examine similarity in species composition for each month, and community structure in Ulleungdo was divided into two groups. Community structures in February, April and December with low temperature and well-mixed surface water were distinguished from those in June, August and October with high temperature and strong stratification, which could be attributed to temporal changes in dominant species. Atka mackerel and Spear squid mainly caught in February and April, disappearing in June, August and October, and File fish outburst was shown in October. Because the water off Ulleungdo has been under low human pressure, it could be a good case study to elucidate effects of climate change on community structure and ecosystem in the East sea. Continuous surveys and further studies are required to demonstrate migration route and distribution of dominant species and long-term changes in community structure in the water of Ulleungdo.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.8
• /
• pp.3543-3549
• /
• 2015

Background: Bladder cancer is one of the most common cancers worldwide. Gene expression profiling using microarray technologies improves the understanding of cancer biology. The aim of this study was to determine the gene expression profile in Egyptian bladder cancer patients. Materials and Methods: Samples from 29 human bladder cancers and adjacent non-neoplastic tissues were analyzed by cDNA microarray, with hierarchical clustering and multidimensional analysis. Results: Five hundred and sixteen genes were differentially expressed of which SOS1, HDAC2, PLXNC1, GTSE1, ULK2, IRS2, ABCA12, TOP3A, HES1, and SRP68 genes were involved in 33 different pathways. The most frequently detected genes were: SOS1 in 20 different pathways; HDAC2 in 5 different pathways; IRS2 in 3 different pathways. There were 388 down-regulated genes. PLCB2 was involved in 11 different pathways, MDM2 in 9 pathways, FZD4 in 5 pathways, p15 and FGF12 in 4 pathways, POLE2 in 3 pathways, and MCM4 and POLR2E in 2 pathways. Thirty genes showed significant differences between transitional cell cancer (TCC) and squamous cell cancer (SCC) samples. Unsupervised cluster analysis of DNA microarray data revealed a clear distinction between low and high grade tumors. In addition 26 genes showed significant differences between low and high tumor stages, including fragile histidine triad, Ras and sialyltransferase 8 (alpha) and 16 showed significant differences between low and high tumor grades, like methionine adenosyl transferase II, beta. Conclusions: The present study identified some genes, that can be used as molecular biomarkers or target genes in Egyptian bladder cancer patients.

• Geophysics and Geophysical Exploration
• /
• v.1 no.3
• /
• pp.170-175
• /
• 1998

A systematic methodology is developed for the prediction of the lithology using electrofacies classification from wireline log data. Multivariate statistical techniques are adopted to segment well log measurements and group the segments into electrofacies types. To consider corresponding contribution of each log and reduce the computational dimension, multivariate logs are transformed into a single variable through principal components analysis. Resultant principal components logs are segmented using the statistical zonation method to enhance the quality and efficiency of the interpreted results. Hierarchical cluster analysis is then used to group the segments into electrofacies. Optimal number of groups is determined on the basis of the ratio of within-group variance to total variance and core data. This technique is applied to the wells in the Korea Continental Shelf. The results of field application demonstrate that the prediction of lithology based on the electrofacies classification works well with reliability to the core and cutting data. This methodology for electrofacies determination can be used to define reservoir characterization which is helpful to the reservoir management.

• Korean Journal of Acupuncture
• /
• v.38 no.2
• /
• pp.100-109
• /
• 2021

Objectives : Source acupoint is one of the representative acupoints to treat various diseases in each meridian. We aimed to identify the patterns of selection of Source acupoints and their associations with diseases using clinical trials data. Methods : We extracted the frequency of Source acupoints across 30 diseases from clinical trials database. Acupuncture treatment regimens were retrieved from the Cochrane Database of Systematic Reviews. The frequency of Source acupoint use was calculated as the number of studies using a certain acupoint divided by the total number of included studies. Using hierarchical clustering and multidimensional scaling, the characteristics of Source acupoints were analyzed based on the similarity of the relationships between the Source acupoints and the diseases. Results : A total of 421 clinical trials were included for this analysis. LR3, HT7, KI3, and LI4 acupoints were most frequently used for the treatment of 30 diseases. Cluster analysis showed that LR3 and LI4 acupoints were grouped together and HT7 and KI3 acupoints were grouped together. Multidimensional scaling revealed that LR3, LI4, HT7, and KI3 acupoints have intrinsic properties in the two-dimensional space. Conclusions : The present study identified the selection patterns of the Source acupoints using clinical trials data. Our finding will provide the understanding of the characteristics of Source acupoints.

• Journal of Convergence for Information Technology
• /
• v.11 no.2
• /
• pp.130-137
• /
• 2021

The purpose of this study is to explore methods to prevent and improve the problem of overdependence on smartphones by analyzing the differences in satisfaction with college life satisfaction of freshmen. For this, data on freshmen of KCYPS were extracted and used. The data were analyzed using non-hierarchical cluster(K-means) analysis, T-test, one-way ANOVA, and Scheffé tests. The results of this study are as follows. First, it has been shown that freshmen who are overdependent on smartphones experience inconvenience in their daily life, withdrawal and anxiety, and resistance. Second, there is no significant difference in the level of satisfaction with college life according to gender(t=-.015, p<.05). Third, it is shown that the level of satisfaction with college life of overdependent freshmen on smartphone is significantly lower compared to that of average and moderate dependent freshmen. Based on the results, it was suggested that university-level efforts such as emotional support and learning strategy support are needed for freshmen who are overdependent on smartphones.