The objective of this study was to investigate the cause of anthocyanin accumulation in the corn leaves treated with metsulfuron-methyl. The accumulation of anthocyanin and total sugar was increased with the time after metsulfuron-methyl application and with the greater herbicide concentration as well. The anthocyanin increase was alleviated by the combined treatment of DUMU or the tank-mixture treatment of metsulfuron-methyl, isoleucine and valine. Metsulfuron-methyl could not enhance the anthocyanin formation in the nonchlorophyllous tissue in which photosynthetic carbohydrate production was limited. Upon the exogenous supply of sucrose, however, the contents of anthocyanin was increased in the both chlorophyllous and nonchlorophyllous leaf segment of corn without the herbicide treatment. On the other hand, the herbicide itself did not influence Hill reaction in vitro and photosynthesis electron transport in vivo. The anthocyanin accumulation by metsulfitronmethyl did not occur in the corn mutants deficient in the structural gene of anthocyanin synthesis, but corns deficient in the regulatory gene had the anthocyanin accumulation only in R-r and r-r type. The above results suggest that the purple pigmentation in the corn leaves treated with metsulfuron-methyl is related to the accumulation of photosynthetic carbohydrate which can stimulate the. regulatory gene related to anthocyanin biosynthetic pathway.
In spite water direct seeding cultivation of rice has advantages in terms of laborsaving and cost-down, it demonstrates uneven seedling establishment and difficult weed management. To select an applicable mat for mulching system of water seeded rice, weed occurrence, growth and yield performances of rice, and soil temperature were evaluated in several kinds of biodegradable mulching material. Field studies were conducted at the experimental farm of National Crop Experiment Station in 1999 and 2000. Among the mats tested in 1999, Safer-mat had the greatest effect on inhibiting weed occurrence and Lawn-mat demonstrated the least efficacy of weed control (EWC) value. In Safer-mat treatment, Ludwigia prostrata was the dominant weed species but Echinochloa crus-galli was not observed. Different kinds of mulching material from the mats studied in 1999 were tested in 2000. Biodegradable polyester (PES) coated on the recycled paper for newspaper press (RP) treatments showed similar or higher EWC values than non-mulched broadcast seed rice with 2 times of herbicide application (BC-herbicide) and Safer-mat that was the most effective mat on preventing weed appearance in 1999. Immediately after mat mulching on the surface of paddy soil, five pregerminated rice seeds were manually sown in each 3cm-hole formerly punctured at 30${\times}$14 cm spacing in 2000. All the 3 kinds of mulching material tested in 2000 had better seedling stand than BC-herbicide treatment. Rice yield in PES coated with 10 thickness on RP (PES10 $\mu\textrm{m}$+RP) was a little higher than in BC-herbicide treatment but the former produced less panicles in unit land area than the latter. PES10 $\mu\textrm{m}$+RP demonstrated a great effect on raising soil surface temperature than non-mulching treatment throughout the period of seedling establishment.
This experiment was conducted to investigate the loss of various herbicides by means of vola-tility from the turfgrass field and the hare ground with the different soil moisture contents and temperatures. Different herbicides were applied at the rates of 375 g a.i. /l0a of pendimethalin,250 g a.i. /l0a of napropamide, and 96.4 g a.i. /l0a of dicamba with 200 \ulcorner/10a of spray volume in the turfgrass(Zoysia japonica cut off 5cm) grown in pots(265.8 $cm^2$) and bared soil. The pots were placed in the growth chamber with 10,000 lux of light intensity(12h per day) at 25 and 35˚C for 7days. Amberlite XAD polymeric resin(20/50 mesh) was used as sampling media for herbicide airborne residues. Air flow was maintained at 10 \ulcorner /min by vacuum pump regulated with a factory calibrated flow meter. Herbicide airborne residues were extracted from the XAD resin with 300 ml of 1:1 acetone and hexane. The extracts were concentrated by rotary evaporation at 35˚C and dissolved in 1 ml MeCN for HPLC analysis. The airborne losses of the herbicide applied in the turfgrass and bare soil increased as the temperature and soil moisture contents were increased, regardless of the kinds of herbicide. Higher airborne residues was observed in the turfgrass pots than the bare soil pots. Pendimethalin and dicamba with higher vapor pressure gave rise to the increased loss of airborne herbicides, showing that 6.26 and 6.4% of average airborne loss in pendimethalin and dicamba, respectively, compared to 0.56% in napropamide. The amount of airborne losses in turfgrass was highest at one day after application and then a declined trend was observed as the time was prolonged. Key words. Herbicides, Turfgrass field, Bare ground, Volatility.
Journal of The Korean Society of Grassland and Forage Science
/
v.11
no.2
/
pp.102-107
/
1991
This trial was carried out to investigate the effect of oversowing on the fodder value and the life form of standing crop in the low productive grassland with a western exposure at the Korean Native Cattle Improvement Center in Seosan. Chungnam Province. The results obtained are summarized as follows: 1. The inferior plant community of low productive grassland was improved to the most desirable pasture association by oversowing after herbicide application. At the experimental area most of the native grasses were not suitable for the pasture plant due to less fodder value(0-2). 2. The fodder value of standing crop in the improved grassland with 6.04(grasses: 4.72, legumes: 1.07 and herbs: 0.25) was much more increased by 108% than that of low productive grassland. 3. The life forms of plant community in the low productive grassland were composed of Hemicryptophytes of 36.29'0, Therophytes of 30.47~, Chamaephytes of 17.0% and Geophytes of 16.4%). But by oversowing after herbicide application their life forms of Hemicryptophytes increased greatly by 51.4%, on the other hand Therophytes, Chamaephytes and Geophytes were much more decreased by 25.4, 15.6 and 10.470, respectively.
An experiment was conducted at the Yeongnam Crop Experiment Station for 4 years from 1985 to understand the effect of repeated use of same herbicide on weed growth. For this, the most common 6 herbicides, butachlor, bifenox, butachlor/pyrazolate, piperophos/dimethametryne, bentazon and propanil were used as single treatment or combination treatment. For non chemical plots, weed occurrence was rapidly increased with time in terms of number and weight. However no rice plot recorded the least increasing ratio. Similar trend was obtained at the herbicide plot even though the increasing ratio varied by herbicide properties. The least increasing ratio (1200 was recorded at the plot of butachlor/pyrazolate (5 days after transplanting, 5 DAT) followed by bentazon+propanil(25 DAT) while the greatest ratio (28%) was obtained from the plot of bifenox (5 DAT). In general, Scirpus hotarui, Eleocharis kuroguwai and Cyperus serotinus was gradually inereased with time by herbicide application, particularly at the butachlor/pyrazolate treatment. The community dominance and simpson index increased at the plot of bentazon and propanil due to increased of particular species while other herbicide plots exhibited reverse trend. Grain yield was curvilinearly decreased with weed weight for both times, 40 DAT and heading time eventhough the correlation coefficient of heading time was higher than 40 DAT.
This study was conducted to develop a foliar applied herbicide mixture covering wide spectrum weeds in dry direct seeded rice. Uniform precision central composite design(UPCCD) was employed to evaluate the effect of herbicide mixture among cyhalofop-butyl(cyhalofop), bispyribenzoxim and pyrazosulfuron-ethyl(pyrazosulfuron) having different weed control spectrum and to determine the best application rates of the three herbicide mixtures. The partial additive effect was observed in the mixture of cyhalofop and bispyribenzoxim with pyrazosulfron against Echinochloa crus-galli Beauv var crus-galli L., attributed to the negative interaction between cyhalofop and bispyribenzoxim system. Additive effect was observed in the mixture of bispyribenzoxim and pyrazosulfuron against Cyperus serotinus R. and Aneilema keisak H. but cyhalofop had no effect on these weeds. The most appropriate rate of the mixture of cyhalofop with bispyribenzoxim and pyrazosulfuron was 100 : 12 : 10g ai/ha exhibiting $ED_{90}$ against three weeds such as E. crus-galli, C. serotinus and A. keisak.
This study was conducted to develop a 2 row type band furrow tiller for a walking cultivator. The tillage and weeding operations in the furrow of dry fields has been done manually or chemical herbicide has been applied. The application of herbicide induces soil pollution and manual operation requires heavy labor. This 2-row type implement was developed to substitute this manual operation and to minimize soil pollution. The developed implement was composed of power transmitting device, tilling device, frame and tail wheel. The max. plowing width and depth were 300mm and 180mm, respectively. The revolutions of the hexagonal shaft and the tillage shaft were $227{\sim}376rpm$ and $355{\sim}590rpm$, respectively. The adequate working speed was $0.50{\sim}0.83m/s$ and the field capacity was $0.17{\sim}0.28hr/10a$.
Several rice herbicides were evaluated to determine the herbicidal efficacy on dry seeded paddy rice. The time of herbicide application was within 1 day after seeding(DAS), 18 DAS, and 40 DAS. Under the upland conditions 30 DAS(before permanent flooding) dominant weed species were Echinochloa sp., Digitaria sp., Leptochla sp., weedy rice and other several annual broadleaved weeds belonging to Cruciferae, but after flooding Echinochloa sp., Aeschynomene sp., Eleocharis kuroguwai, Cyperus sorotinus, Ludwigia prostrata, Aneilema japonica were dominat. Early application (1 DAS) of thiobencarb, pyrazolate/butachlor, or chlomethoxyfen/butachlor resulted in very good herbicidal efficacy only during the first 30 days, but not thereafter. Application of propanil+butachlor on 18 DAS did not control the late occurring weeds effectively. Application of bentazone/quinchlorac on 40 DAS successfully suppressed weed growth throughout the remaining rice growing season and showed similar grain yield as hand weeded plot. Weed growth reduced the plant height, panicle number, 1000-grain weight, percent ripened grains, and grain yield of rice by 7, 82, 19, 55 and 87% respectively. There was a positive linear relationship between weed weight and lodging index of rice.
This study was conducted to investigate the phytotoxic reaction and the impact on the growth of apple fruit included the yield amount by misuse of non-selective herbicide, glufosinate-ammonium in apple field and for the study, 4 cultivars of apple were tested. The results showed that the highest phytotoxic degree was observed on Sansa-cultivar and then the degree was diminished on Tsugaru, Hongro and Fuji in order in the application year. In one year after application, the highest phytotoxic degree was showed on Fuji and then diminished on Tsugaru, Hongro and Sansa in order. However, in one year after application, the phytotoxic symptom was negligible in comparison with the degree in the year of application and the flowering and fruiting were reduced 30% only in Fuji, but in the other cultivars, the phytotoxic symptoms were not observed. Meanwhile, the residue of glufosinate-ammonium was below the detection limit as 0.04ppm in all tested cultivars and in 2years after application, the phytotoxic symptom was not observed in all cultivars of the apple.
Jung, Jeong Sung;Lee, Ki-Won;Choi, Ki Choon;Ji, Hee Jung;Park, Hyung Soo;Kim, Won Ho;Kim, Young-Jin;Lee, Sang Hak;Lee, Sang-Hoon
Journal of The Korean Society of Grassland and Forage Science
/
v.35
no.1
/
pp.17-25
/
2015
The following experiments were used to evaluate the effect of seeding method and pre-emergence herbicides on weed control and the production of the $sorghum{\times}sudangrass$ [sorghum bicolor (L.) Moench] hybrid under field and in vitro conditions. Under the in vitro condition, we evaluated how different kinds of herbicides affect the rate of seed germination and the growth of early seedlings in the $sorghum{\times}sudangrass$ hybrid treated with herbicides. Simazine, alachlor, and methabenzthiazuron were used, and their concentration levels were 0%, 40%, 70%, and 100%. The germination ratio of the seeds was reduced by the application of simazine, methabenzthiazuron, and alachlor compared with the control (distilled water). The alachlor application severely decreased the sorghum germination ratio. However, seed germination was not greatly influenced by the concentration level of the herbicides. Root and shoot length were increased in the following order: control > simazine > methabenzthiazuron > alachlor. Overall, alachlor significantly reduced root length more than shoot length. The differential tolerance of the $sorghum{\times}sudangrass$ hybrid to pre-emergence herbicides by seeding method was then tested under the field condition. The plants were severely injured by the alachlor application, leading to a great reduction in the number of stems. However, for the simazine and methabenzthiazuron applications the number of stems lost was slightly lower than it was for the alachlor and simazine + alachlor applications. In the seeding method, drilling was more effective in reducing herbicide injury than broadcasting. All of the herbicides effectively controlled weeds. The simazine and methabenzthiazuron applications showed a significantly higher dry matter (DM) yield. However, the alachlor and simazine + alachlor applications severely reduced the DM yield. This result suggests that the alachlor application has a significant effect on plant production. Similarly, the total digestible nutrient (TDN) yield was greatly reduced by the alachlor and simazine + alachlor applications. From the results presented above, we concluded that the yield of DM was the highest for broadcasting with no herbicide. and drilling was the most-effective way to reduce herbicide injury. The simazine application was the most-effective herbicide, showing the highest forage yield and TDN yield.
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