• 한국어병학회지
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• 제36권2호
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• pp.369-378
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• 2023

In this study, clinically healthy Korean rockfish were provided by a farm and then raised in a lab for 14 days at three different temperatures (10℃, 15℃, and 20℃) to establish hematological, blood biochemical, and histopathological reference intervals against normal fish. Hematocrit, MCV, MCHC, total protein, BUN, and GPT values in the blood showed significant differences among temperature groups. As the water temperature increased, neutrophil, thrombocyte, and lymphocyte counts also rose, while the monocyte value peaked at 15℃. The histopathological score revealed significant variations in the gills, stomach, and inflammation indices by temperature group; the gills and inflammation indices peaked at 20℃, whereas the stomach index peaked at 15℃. It is expected that information on these normal values will serve as a fundamental collection of data for further studies related to laboratory-based experiments.

• Journal of Yeungnam Medical Science
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• 제8권1호
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• pp.154-165
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• 1991

1) 건강한 성인 2823명을 대상으로 자동혈구 계산기인 Coulter Counter Model S plus II를 이용하여 본원의 참고치를 설정하였다. 산출된 참고치는 기기의 설명서에 제시되어 임상 의사들이 사용하고 있는 참고치와 차이가 있었다. 2) 국내의 보고들과 비교해 볼 때 본원의 참고치와 대체로 비등하였고 기기의 설명서에 제시된 참고치와는 차이가 있으므로 본원의 참고치률 설정하여 사용하여야 하겠다. 3) 결과는 다음과 같다. (1) 백혈구 수는 남자 $6.800{\pm}2.680(2SD)/{\mu}l$, 여자 $5.950{\pm}2.380(2SD)/{\mu}l$ 이었다. (2) 적혈구 수는 남자 $428{\pm}60(2SD){\times}10^4/{\mu}l$, 여자 $415{\pm}56(2SD){\times}10^4/{\mu}l$ 이었다. (3) 혈색소량은 남자 $15.4{\pm}1.8(2SD)g/dL$, 여자 $13.0{\pm}1.6(2SD)g/dL$ 이었다. (4) 적혈구 용적은 남자 $45.3{\pm}5.0(2SD)%$, 여자 $38.2{\pm}4.6(2SD)%$ 이었다. (5) 평균 적혈구 용적은 남자 $93.8{\pm}5.8(2SD)fL$, 여자 $92.2{\pm}7.4(2SD)fL$ 이었다. (6) 평균 적혈구 혈색소량은 남자 $31.8{\pm}2.2(2SD)pg$, 여자 $31.4;{\pm}2.8(2SD)pg$ 이었다. (7) 평균 적혈구 혈색소농도는 남자 $34.0{\pm}1.2(2SD)%$, 여자 $33.9{\pm}1.2(2SD)%$ 이었다. (8) 적혈구 분포 폭은 남자 $12.7{\pm}1.0(2SD)%$, 여자 $12.6{\pm}1.4(2SD)%$ 이었다. (9) 혈소판 수는 남자 $242.9{\pm}87.8(2SD){\times}10^4/{\mu}l$, 여자 $242.2{\pm}89.0(2SD){\times}10^4/{\mu}l$ 이었다. (10) 혈소판 용적은 남자 $0.201{\pm}0.076(2SD)%$, 여자 $0.204{\pm}0.076(2SD)%$ 이었다. (11) 평균 혈소판 용적은 남자 $8.20{\pm}1.70(2SD)fL$, 여자 $8.36{\pm}1.82(2SD)fL$ 이었다. (12) 혈소판 분포 폭은 남자 $16.1{\pm}0.8(2SD)%$, 여자 $16.0{\pm}0.8(2SD)%$ 이었다.

• 한국임상수의학회지
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• 제23권2호
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• pp.111-113
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• 2006

수컷 엘크 사슴 85두에서 채혈하여 한국에서 사육되는 엘크사슴의 혈액화학치에 대한 정상범위를 설정하였다. 적혈구수에 대한 참고범위는 6.01-9.93 (${\times}10^6/mm^3$)이었으며, 헤모글로빈농도에 대하여는 10.90-19.70 (g/dl), 적혈구용적은 43.10-76.40 (%), 총백혈구수에 대하여는 2.60-7.68 (${\times}10^6/mm^3$)으로 산정되었다. 위의 네가지 혈액화학치에 관하여 한국 및 외국에서 기발표된 자료와 비교하면서 본 연구에서 설정된 참고범위의 값들의 타당성을 검증하였다.

• 한국동물위생학회지
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• 제42권4호
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• pp.193-200
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• 2019

Blood test is a useful tool in establishing medical treatment for livestock. It provides information such as disease diagnosis, treatment effects, prognostic judgment, and health status. This study compared the value of erythrocytes and leukocytes among conventional, transgenic miniature, and transgenic conventional pigs aged six months to 24 months. Further, it analyzed the aspects of hematological value changes according to the pigs' ages. As a result, the number of red blood cells (RBC), which include hemoglobin, and hematocrit, and the number of white blood cells (WBC), which include neutrophils, and lymphocyte, were high among transgenic miniature pigs, compared with the conventional and transgenic conventional pigs. Conventional pigs showed similar values of RBC and WBC regardless of transgenesis. In comparing their age, the RBC decreased as the age increased compared with the pigs among all the three groups aged of 6~12 months. On the other hand, WBC and neutrophils showed no significant difference regardless of different ages among all the three groups. However, various counts in RBC and WBC were mostly found to be higher in each age in transgenic miniature pigs than in conventional and transgenic conventional pigs. The results of this study show that the values of RBC and WBC were generally higher in transgenic miniature pigs than in conventional and transgenic conventional pigs. Based on this research, hematological values can be widely used in diagnosing diseases or checking the health status of transgenic pigs that are used as disease models, organ transplant source and alike.

• 한국동물위생학회지
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• 제40권2호
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• pp.155-159
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• 2017

Korean goral (Neamorhedus caudatus) is registered as a natural monument number 217 by South Korea Cultural Heritage Administration. It is also recognized as the endangered species I by Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). In this study, blood samples of Korean goral were collected to make reference intervals. Blood sampling was conducted on 19 numbers of Korean gorals (ten females, nine males), which were reared in Korean Goral Restoration Center. For total samples, the reference intervals of hematological parameters were: white blood cell $7.69{\sim}10.87K/{\mu}{\Lambda}$; hematocrit 36.73~46.18%; red blood cell $10.72{\sim}12.86K/{\mu}{\Lambda}$; hemoglobin 12.79~15.14 g/dL; mean corpuscular volume 33.15~36.75 fL; mean corpuscular hemoglobin 11.53~12.23 pg; mean corpuscular hemoglobin concentration 32.64-35.91 g/dL and red blood cell distribution width 39.2~43.46%. For total samples of each parameters, the following results were obtained for serum biochemistry: glucose 111.81~153.77 mg/dL; blood urea nitrogen 22.35~28.91 mg/dL; creatine 1.22~1.84 mg/dL; phosphate 4.57~6 mg/dL; calcium 8.7~9.1 mg/dL; total protein 6.53~6.92 g/dL; albumin 3.1~3.48 g/dL; globulin 3.26~3.62 g/dL; alanine aminotransferase 56.7~158.56 U/L; aspartate aminotransferase 230.35~473.06 U/L; alkaline phosphatase 178.06~332.47 U/L; gamma-glutamyl transpeptidase 131.6-~181.24 U/L; total bilirubin 1.47~2.12 mg/dL; cholesterol 46.48~71.52 mg/dL; amylase 16.3~26.03 U/L; sodium 150.43~153.88 mmol/L; potassium 3.98~4.6 mmol/L and chlorine 109.48~113.26 mmol/L. The ranges of values were similar campared to previous studies except in the case of RDW value, which showed higher range than the RDW value of a previous study. The reference intervals from this study will be useful data for treatment and management of gorals.

• 대한한방내과학회지
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• 제40권4호
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• pp.582-596
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• 2019

Objective: The aims of this study were to analyze the deficiency-excess pattern identification (虛實辨證) and compare it to the sputum cytokines of asthma patients. Method: 50 asthma patients who met the inclusion and exclusion criteria were included in this study. They were divided into two groups: deficiency and excess syndrome groups. Sputum examinations were performed including $TNF-{\alpha}$, Interleukin (IL)-4, IL-5, IL-10, and IL-13. The Quality of Life Questionnaire for Adult Korean Asthmatics (QLQAKA), the Visual Analog Scale(VAS), and heart rate variability (HRV) were also measured. We also conducted laboratory tests, including the hematological indexes. Results: Based on the pattern identification, 50 asthma patients can be divided into two categories of groups: the deficiency syndrome group (N=24) and the excess syndrome group (N=26). In the analysis of sputum cytokines, although the $TNF-{\alpha}$, IL-4 and IL-13 were at a higher level in the deficient pattern group than in the excess pattern group, it was insignificantly different. There was a negative correlation in the analysis of QLQAKA and VAS. In the analysis of HRV, although the mean value of VLF, LF, and HF in the deficiency syndrome group was higher than in the excess syndrome group, it was insignificantly different. There was no significant difference in the hematological tests between the deficiency and the excess syndrome group. The mean value of the IgE in the blood tests was five times greater than the reference value. Conclusion: The cytokines of sputum including $TNF-{\alpha}$, IL-4, IL-5, IL-10, and IL-13 were indifferent statistically. Reinforcing the healthy and eliminating the pathogenic factors should be considered.

• 한국어병학회지
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• 제19권3호
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• pp.253-265
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• 2006

본 연구는 동물용 생화학 분석장치를 이용하여 분석한 어류 혈액검사 항목 가운데 적용할 수 있는 분석항목의 활용도를 평가함과 동시에 어류 혈액학적 건강진단에 필요한 분석항목의 참고자료를 얻고자 하였다. 경남 지역의 해상가두리 어류 양식장에서 사육중인 육안적으로 보아 건강한 조피볼락 (Sebastes schlegeli), 참돔 (Pagrus major), 돌돔 (Oplegnathus fasciatu) 및 감성돔 (Acanthopagrus schlegeli) 총 522마리를 대상으로 hematocrit 와 간편하게 혈액성분을 측정할 수 있는 FUJI DRI-CHEM 3000 기종을 이용하여 혈장화학적 성분 분석을 실시하였다. Hematocrit (Ht)는 microhematocrit법에 의해 그리고 hemoglobin (Hb), 총단백 (TP), 알부민 (ALB), 알칼리성포스파타제 (ALP), 요소질소 (BUN), 젖산탈수소효소 (LDH), 중성지방 (TG), 총콜레스테롤 (TCHO), 크레아티닌 (CRE), aspartate aminotransferase (AST), alanine aminotransferase (ALT), 포도당(GLU)은 정상범위의 표준자료가 이미 프로그램화되어 장치된 동물용 생화학 분석장치인 FUJI DRI-CHEM 3000 기종을 이용하여 분석하였다. 그 결과, 측정된 Hb와 혈장화학성분의 실제 값들은 본 기종의 하한 및 상한 한계치를 상당히 벗어났다. 본 장비로부터 분석값을 검출하지 못하였던 비율을 살펴보면, ALB 및 ALT는 68～66%, LDH, TP, ALP 및 GLU는 42～21%, TCHO, AST, TG, Hb 및 CRE는 18～3%의 범위를 나타내었으나, BUN의 분석값은 모두 검출한계를 벗어나지 않았다.

• 대한한방내과학회지
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• 제38권6호
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• pp.955-970
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• 2017

Objectives: The aim of this observational study was to identify and analyze the patterns to compare the characteristics of asthma patients. Methods: The subjects were 40 asthma patients who had satisfied the inclusion and exclusion criteria. They were divided into deficiency syndrome and excess syndrome groups, and cold syndrome and heat syndrome groups. Their quality of life was measured by the quality of life questionnaire for adult Korean asthmatics (QLQAKA) and VAS. Heart rate variability (HRV) was measured, and the degree of obesity was evaluated by body mass index (BMI). Hematological, biochemical, and immunoglobulin (Ig) E laboratory tests were included. Results: Based on pattern identification, the 40 asthma patients could be divided into two categories of groups: 1) the deficiency syndrome (N=18) and the excess syndrome (N=22) groups: 2) the cold syndrome (N=35) and the heat syndrome (N=5) groups. The mean value of HF differed significantly between the deficiency and excess syndrome groups. The mean value of IgE in blood tests of asthmatics was greater than four times the reference value. For BMI, the subjects were classified into three groups: normal weight (N=12), overweight (N=12), and obese (N=16). Conclusions: Development of a more accurate asthma-specific pattern identification tool could play a crucial role in asthma control. In addition, good control of asthma can improve the quality of life. Obesity is one of the factors associated with asthma exacerbation.

• 한국산업보건학회지
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• 제10권2호
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• pp.109-123
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• 2000

Inhaled halogen compound was examined through micronucleus tests and toxicity tests using SD (Sprague-Dawley) rats with repeated dosages for six hours a day, five days a week, during four weeks. In four-week repeated exposure, no specific sign caused by the $CF_3I$ compound was observed on the clinical symptoms, body weight variation, feed consumption, and urinalysis data in the testing groups with reference to the control group. In hematological and biochemical blood tests of the testing groups, the significant, but in their normal ranges, value dependencies of glucose, aspartate aminotransferase, alanine aminotransferase and etc., on the halogen carbohydride concentration was observed. In histo-pathological tests, no specific lesion or concentration dependent change due to the $CF_3I$ compound dosage was observed in both sexes of the female and male in the tested animals. But, micronucleus tests on marrow cells extracted from the tested animals which were repeatedly exposed in the $CF_3I$ compound during four weeks, the frequencies of micronuclei were significantly increased dose-dependently compared to the control groups.