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Reference intervals of hematological and histopathological index at three different temperatures in Korean rockfish Sebastes schlegelii

조피볼락의 수온별 혈액학적 및 병리조직학적 정상치 탐색

  • Beom Hee Lee (Department of Aquatic Life Medicine, ONSE College, Kunsan National University) ;
  • Sang Bin Lee (Department of Aquatic Life Medicine, ONSE College, Kunsan National University) ;
  • Eul Bit Noh (Department of Aquatic Life Medicine, ONSE College, Kunsan National University) ;
  • Ji Min Ryu (Department of Aquatic Life Medicine, ONSE College, Kunsan National University) ;
  • Bo Seong Kim (Department of Aquatic Life Medicine, ONSE College, Kunsan National University)
  • 이범희 (군산대학교 수산생명의학과) ;
  • 이상빈 (군산대학교 수산생명의학과) ;
  • 노을빛 (군산대학교 수산생명의학과) ;
  • 류지민 (군산대학교 수산생명의학과) ;
  • 김보성 (군산대학교 수산생명의학과)
  • Received : 2023.11.28
  • Accepted : 2023.12.04
  • Published : 2023.12.31

Abstract

In this study, clinically healthy Korean rockfish were provided by a farm and then raised in a lab for 14 days at three different temperatures (10℃, 15℃, and 20℃) to establish hematological, blood biochemical, and histopathological reference intervals against normal fish. Hematocrit, MCV, MCHC, total protein, BUN, and GPT values in the blood showed significant differences among temperature groups. As the water temperature increased, neutrophil, thrombocyte, and lymphocyte counts also rose, while the monocyte value peaked at 15℃. The histopathological score revealed significant variations in the gills, stomach, and inflammation indices by temperature group; the gills and inflammation indices peaked at 20℃, whereas the stomach index peaked at 15℃. It is expected that information on these normal values will serve as a fundamental collection of data for further studies related to laboratory-based experiments.

Keywords

Acknowledgement

이 논문은 2022학년도 군산대학교 신임교수 연구비 지원에 의하여 연구되었음.

References

  1. Abram, Q. H., Dixon, B. and Katzenback, B. A.: Impacts of low temperature on the teleost immune system. Biology, 6(4), 39. 2017.
  2. Bernet, D., Schmidt, H., Meier, W., Burkhardt-Holm, P. and Wahli, T.: Histopathology in fish: proposal for a protocol to assess aquatic pollution. Journal of fish diseases, 22(1), 25-34. 1999. https://doi.org/10.1046/j.1365-2761.1999.00134.x
  3. Boehlert, G. W. and Yoklavich, M. M.: Effects of temperature, ration, and fish size on growth of juvenile black rockfish, Sebastes melanops. Environmental Biology of Fishes, 8, 17-28. 1983. https://doi.org/10.1007/BF00004942
  4. Cheng, A. C., Cheng, S. A., Chen, Y. Y. and Chen, J. C.: Effects of temperature change on the innate cellular and humoral immune responses of orange-spotted grouper Epinephelus coioides and its susceptibility to Vibrio alginolyticus. Fish & shellfish immunology, 26(5), 768-772. 2009. https://doi.org/10.1016/j.fsi.2009.03.011
  5. Choi, H. S., Myoung, J. I., Park, M. and Cho, M. Y.: A Study on the summer mortality of Korean rockfish Sebastes schlegeli in Korea. Journal of fish pathology, 22(2), 155-162. 2009.
  6. Chun, K. S.: Attachment of the Microcotyle sebastis (Monogenea: Microcotylidae) to the gills of black rockfish, Sebastes melanops. Journal of Environmental Health Sciences, 28(5), 102-105. 2002.
  7. Dai, Y. J., Cao, X. F., Zhang, D. D., Li, X. F., Liu, W. B. and Jiang, G. Z.: (2019). Chronic inflammation is a key to inducing liver injury in blunt snout bream (Megalobrama amblycephala) fed with high-fat diet. Developmental & Comparative Immunology, 97, 28-37. 2019.
  8. Evans, D. H., Piermarini, P. M. and Choe, K. P.: The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiological reviews, 85(1), 97-177. 2005. https://doi.org/10.1152/physrev.00050.2003
  9. Gill III, T. J., Smith, G. J., Wissler, R. W. and Kunz, H. W.: The rat as an experimental animal. Science, 245(4915), 269-276. 1989. https://doi.org/10.1126/science.2665079
  10. Jeney, G., Nemcsok, J., Jeney, Z. S. and Olah, J.: Acute effect of sublethal ammonia concentrations on common carp (Cyprinus carpio L.). II. Effect of ammonia on blood plasma transaminases (GOT, GPT), G1DH enzyme activity, and ATP value. Aquaculture, 104(1-2), 149-156. 1992. https://doi.org/10.1016/0044-8486(92)90145-B
  11. Jia, Y., Wang, J., Gao, Y. and Huang, B.: Hypoxia tolerance, hematological, and biochemical response in juvenile turbot (Scophthalmus maximus. L). Aquaculture, 535, 736380. 2021.
  12. Kim, B. S., Park, J. W., Kang, G. S., Jin, J. H., Roh, H. J., Kim, D. H. Lee, M. K. and Min, D. H.: First report of nocardia infection in cultured Japanese eel, Anguilla japonica. Journal of fish diseases, 41(12), 1921-1927. 2018. https://doi.org/10.1111/jfd.12882
  13. KOSIS (Korean Statistical Information Service). 어류양 식동향조사. 2023. Retrived from https://kosis.kr/statHtml/statHtml.do?orgId=101&tblId=DT_1EZ0008&conn_path=I2.
  14. Martins, M. L., Xu, D. H., Shoemaker, C. A. and Klesius, P. H.: Temperature effects on immune response and hematological parameters of channel catfish Ictalurus punctatus vaccinated with live theronts of Ichthyophthirius multifiliis. Fish & shellfish immunology, 31(6), 774-780. 2011. https://doi.org/10.1016/j.fsi.2011.07.015
  15. MedCalc Software Ltd: MedCalc® Statistical Software version 22.014 (https://www.medcalc.org; 2023).
  16. Nelson, K., Jones, J., Jacobson, S. and Reimschuessel, R.: Elevated blood urea nitrogen (BUN) levels in goldfish as an indicator of gill dysfunction. Journal of Aquatic Animal Health, 11(1), 52-60. 1999. https://doi.org/10.1577/1548-8667(1999)011<0052:EBUNBL>2.0.CO;2
  17. NIFS (National Institute of Fisheries Science). 조피볼락 양식 표준 매뉴얼. 2017.
  18. Retrived from https://www.nifs.go.kr/distantwater/skin/doc.html?fn=20230105102437021_0.pdf&rs=/distantwater/preview/Board0027/
  19. Racicot, J. G., Gaudet, M. and Leray, C.: Blood and liver enzymes in rainbow trout (Salmo gairdneri Rich.) with emphasis on their diagnostic use: Study of CCl4 toxicity and a case of Aeromonas infection. Journal of fish Biology, 7(6), 825-835. 1975. https://doi.org/10.1111/j.1095-8649.1975.tb04653.x
  20. Roychowdhury, P., Aftabuddin, M. and Pati, M. K.: (2020). Thermal stress altered growth performance and metabolism and induced anaemia and liver disorder in Labeo rohita. Aquaculture Research, 51(4), 1406-1414. 2020. https://doi.org/10.1111/are.14486
  21. Saraiva, A., Costa, J., Serrao, J., Cruz, C., and Eiras, J. C.: A histology-based fish health assessment of farmed seabass (Dicentrarchus labrax L.). Aquaculture, 448, 375-381, 2015 https://doi.org/10.1016/j.aquaculture.2015.06.028
  22. Seki, M., Nakayama, M., Sakoh, T., Yoshitomi, R., Fukui, A., Katafuchi, E., Susumu, T., Toshaki, N., Kazuhiko, T. and Kitazono, T.: Blood urea nitrogen is independently associated with renal outcomes in Japanese patients with stage 3-5 chronic kidney disease: a prospective observational study. BMC nephrology, 20, 1-10. 2019. https://doi.org/10.1186/s12882-019-1306-1
  23. Struillou, X., Boutigny, H., Soueidan, A. and Layrolle, P.: Experimental animal models in periodontology: a review. The open dentistry journal, 4, 37. 2010.
  24. Strzyzewska, E., Szarek, J. and Babinska, I.: Morphologic evaluation of the gills as a tool in the diagnostics of pathological conditions in fish and pollution in the aquatic environment: a review. Veterinarni medicina, 61(3). 2016.
  25. Volkoff, H. and Ronnestad, I.: Effects of temperature on feeding and digestive processes in fish. Temperature, 7(4), 307-320. 2020. https://doi.org/10.1080/23328940.2020.1765950
  26. Wolf, S. and Wolff, H. G.: The gastric mucosa, "Gastritis" and ulcer. The American Journal of Digestive Diseases, 10, 23-28, 1943 https://doi.org/10.1007/BF02997406
  27. 김진우, 이한나, 지보영, 우승호, 김영재, 이무근: 한 국 주요 양식종의 수산동물 폐사피해 모니터링. 한국어병학회지, 25(3), 271-277. 2012. https://doi.org/10.7847/jfp.2012.25.3.271
  28. 김흥윤: 수온과 저산소에 따른 조피볼락 (Sebastes schlegeli) 의 호흡대사와 혈액성상의 변화. 한국 어병학회지, 34(2), 213-224. 2021.
  29. 노을빛, 이범희, 진지혜, 김보성: 관상 전기뱀장어 (Electrophorus electricus) 의 피부 궤양병 증례. 한국어병학회지, 36(1), 183-190. 2023. https://doi.org/10.7847/JFP.2023.36.1.183
  30. 박지연, 노형진, 박준우, 정다혜, 이무근, 김도형: 사료 과다공급으로 유도된 비만 무지개송어 (Oncorhynchus mykiss) 의 생리.조직학적 변화. 한국수산과학회지, 55(5), 688-696. 2022. https://doi.org/10.5657/KFAS.2022.0688
  31. 서정수, 이지훈, 지보영, 권문경, 황지연, 황성돈, 정지민, 심재동: 양식생물 9 종의 수산용의약품 사용 현황. 수산해양교육연구, 31(6), 1862-1869. 2019. https://doi.org/10.13000/JFMSE.2019.12.31.6.1862
  32. 양성진, 전제천, 박정준, 명정인, 신윤경: 동합금 가두리 망 사육어류, 조피볼락 (Sebastes schlegeli) 의 혈액 성상과 중금속 축적률. 한국어류학회지, 26(3), 159-170. 2014.
  33. 이동규: 양식 넙치 (Paralichthys olivaceus) 비병증 예에 대한 주요장기의 조직학적 관찰 (Doctoral dissertation, 부경대학교 대학원). 2012.
  34. 이무근: 양식 숭어 녹간증에 대한 병리학적 연구 (Doctoral dissertation, 부경대학교 대학원). 2008.
  35. 조기택, 황윤정, 이상우, 김광일, 정현도: 경기도 내 양식어류에서 분리한 병원성 세균의 Tetracycline 내성 유전자 분포. 한국어병학회지, 34(1), 39-46. 2021. https://doi.org/10.7847/JFP.2021.34.1.039
  36. 조미영, 김광일, 민은영, 정승희: 양식생물의 신종질병 및 국내 대응방안. 해양정책연구, 34(1), 67-88. 2019. https://doi.org/10.35372/kmiopr.2019.34.1.003
  37. 조미영, 하헌주, 민진기, 김태진, 지보영, 박신후, 황성돈, 김광일, 장영환, 박명애: 넙치 양식장의 질병관리등급 평가기준 개선 및 적용. 수산해양교육연구, 27(6), 1646-1655. 2015. https://doi.org/10.13000/JFMSE.2015.27.6.1646
  38. 주민수, 김보성, 김도형, 서정수, 권문경, 지보영, 박찬일: Streptococcus parauberis PH0710에 감염된 강도다리 (Platichthys stellatus)에 대한 항생제 Enrofloxacin 의 유효성 및 안전성 조사. 수산해양교육연구, 31(2), 459-473. 2019. https://doi.org/10.13000/JFMSE.2019.4.31.2.459
  39. 한현자, 송준영, 조미영, 최혜승, 정승희, 서한길: 2013-2016 년 양식 조피볼락 (Sebastes schlegeli) 의 해역별 선충 (Clavinema mariae) 및 병원체 감염 현황. 한국수산과학회지, 53(3), 432-442. 2020. https://doi.org/10.5657/KFAS.2020.0432