• 제목/요약/키워드: Helper

검색결과 563건 처리시간 0.037초

Effect of the anti-IL-17 antibody on allergic inflammation in an obesity-related asthma model

  • Liang, Lin;Hur, Jung;Kang, Ji Young;Rhee, Chin Kook;Kim, Young Kyoon;Lee, Sook Young
    • The Korean journal of internal medicine
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    • 제33권6호
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    • pp.1210-1223
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    • 2018
  • Background/Aims: The co-occurrence of obesity aggravates asthma symptoms. Diet-induced obesity increases helper T cell (TH) 17 cell differentiation in adipose tissue and the spleen. The 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitor pravastatin can potentially be used to treat asthma in obese patients by inhibiting interleukin 17 (IL-17) expression. This study investigated the combined effects of pravastatin and anti-IL-17 antibody treatment on allergic inflammation in a mouse model of obesity-related asthma. Methods: High-fat diet (HFD)-induced obesity was induced in C57BL/6 mice with or without ovalbumin (OVA) sensitization and challenge. Mice were administered the anti-IL-17 antibody, pravastatin, or both, and pathophysiological and immunological responses were analyzed. Results: HFD exacerbated allergic airway inflammation in the bronchoalveolar lavage fluid of HFD-OVA mice as compared to OVA mice. Blockading of the IL-17 in the HFD-OVA mice decreased airway hyper-responsiveness (AHR) and airway inflammation compared to the HFD-OVA mice. Moreover, the administration of the anti-IL-17 antibody decreased the leptin/adiponectin ratio in the HFD-OVA but not the OVA mice. Co-administration of pravastatin and anti-IL-17 inhibited airway inflammation and AHR, decreased goblet cell numbers, and increased adipokine levels in obese asthmatic mice. Conclusions: These results suggest that the IL-17-leptin/adiponectin axis plays a key role in airway inflammation in obesity-related asthma. Our findings suggest a potential new treatment for IL-17 as a target that may benefit obesity-related asthma patients who respond poorly to typical asthma medications.

유치원교사의 셀프리더십과 교사전문성의 관계: 교사효능감의 매개효과를 중심으로 (A Study on the Relationship between Self-Leadership and Teacher-Expertise in Kindergarten Teachers: Focusing on the Mediating Effect of the Teachers' Self-efficacy)

  • 최미애;김순영;최양미
    • 한국산학기술학회논문지
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    • 제20권1호
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    • pp.248-258
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    • 2019
  • 본 연구는 유치원에서 자유선택활동을 실시하는 교사의 전문성 향상을 위해 셀프리더십과의 관계를 살펴보고, 교사 효능감에 대한 매개효과를 분석하는 데 목적이 있다. 본 연구는 2018년 9월 17일부터 28일까지 서울 경기 지역 유치원 교사 총 325명에게 방문 조사를 통해 설문을 실시한 자료를 사용하였다. 자료 분석을 위해 SPSS Statistics 22.0과 AMOS 20.0 통계프로그램을 사용하여, 신뢰성분석, 확인적 요인분석, 상관관계 분석, 구조방정식 모형을 실시하였다. 가설검증 결과, 자유선택활동을 교육하는 유치원 교사의 셀프리더십은 교사효능감에 정(+)의 영향을 미치는 것으로 나타났으며, 교사 전문성에도 정(+)의 영향을 미치는 것으로 나타났다. 또한 유치원 교사의 교사효능감도 교사의 전문성에 정(+)의 영향을 미치는 것으로 나타났다. 또한 유치원 교사의 셀프리더십이 교사효능감을 매개하여 교사 전문성에 간접효과가 통계적으로 유의하게 있는 것으로 나타났다. 또한 유치원 교사의 셀프리더십이 교사 전문성에 미치는 직접효과가 유의하게 나타났으며, 이는 유치원 교사의 교사효능감이 셀프리더십과 교사 전문성 간의 관계를 간접 매개함을 확인하였다. 본 연구의 결과를 통하여 유치원 교사의 전문성을 향상시키기 위해서는 교사의 셀프리더십을 강화하는 것은 물론이고, 교사들의 교사효능감 수준을 강화시키기 위해서는 업무환경에 대해 교사들에게 효능감을 높이기 위해 필요한 지식 및 기술 등을 제공하고 교사들의 전문성을 향상시키기 위한 적극적인 촉진자 및 조력자의 역할이 필요할 것이다.

Immunomodulatory Properties of Lactobacillus plantarum NC8 Expressing an Anti-CD11c Single-Chain Fv Fragment

  • Liu, Jing;Yang, Guilian;Gao, Xing;Zhang, Zan;Liu, Yang;Yang, Xin;Shi, Chunwei;Liu, Qiong;Jiang, Yanlong;Wang, Chunfeng
    • Journal of Microbiology and Biotechnology
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    • 제29권1호
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    • pp.160-170
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    • 2019
  • The lactic acid bacteria species Lactobacillus plantarum (L. plantarum) has been used extensively for vaccine delivery. Considering to the critical role of dendritic cells in stimulating host immune response, in this study, we constructed a novel CD11c-targeting L. plantarum strain with surface-displayed variable fragments of anti-CD11c, single-chain antibody (scFv-CD11c). The newly designed L. plantarum strain, named 409-aCD11c, could adhere and invade more efficiently to bone marrow-derived DCs (BMDCs) in vitro due to the specific interaction between scFv-CD11c and CD11c located on the surface of BMDCs. After incubation with BMDCs, the 409-aCD11c strain harboring a eukaryotic vector pValac-GFP could lead to more efficient expression of GFP compared with wild-type strains shown by flow cytometry analysis, indicating the enhanced translocation of pValac-GFP from L. plantarum to BMDCs. Similar results were also observed in an in vivo study, which showed that oral administration resulted in efficient expression of GFP in both Peyer's patches (PP) and mesenteric lymph nodes (MLNs) within 7 days after the last administration. In addition, the CD11c-targeting strain significantly promoted the differentiation and maturation of DCs, the differentiation of $IL-4^+$ and $IL-17A^+$ T helper (Th) cells in MLNs, as well as production of $B220^+$ $IgA^+$ B cells in the PP. In conclusion, this study developed a novel DC-targeting L. plantarum strain which could increase the ability to deliver eukaryotic expression plasmid to host cells, indicating a promising approach for vaccine study.

황련 추출물의 아토피피부염 유발 생쥐에서 피부손상 완화 효과 (Effect of Coptidis Rhizoma extract on Atopic Dermatitis-like Skin Lesions in NC/Nga Mice)

  • 정아람;안상현;정한솔;김기봉
    • 동의생리병리학회지
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    • 제33권2호
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    • pp.102-108
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    • 2019
  • This study aims to evaluate the anti-inflammatory effect of Coptidis Rhizoma (CR) extract for atopic dermatitis through maintaining skin barrier and regulating Th2 cell differentiation. We divided NC/Nga mice into 3 groups as follows; atopy-like dermatitis induced group with CR treatment (CT, n=10), no treatment group(Ctrl), atopy-like dermatitis elicited group(AE). Atopy-like dermatitis was induced to NC/Nga mice by sensitizing with dermatophagoides farinae(DfE) on 7, 8, 9, 11, 12, and 13th week. After inducing atopic dermatitis, CR extract was administered 20 mg/kg daily for the experimental duration to the CT group. We measured the integrity of lipid layers in the epidermis and Th2 differentiation through immunohistochemical staining against filaggrin, loricrin, IL-4, and IL-13. We also measured the distribution of subcutaneous collagen fibers by the Masson's trichrome staining. Administration of CR significantly inhibited the reduction of lipid layers in the skin that caused atopy. The expression of IL-4, IL-13, each of which is a cytokine secreted by T helper type 2 (Th2) cells, was markedly suppressed in the CT group as compared with AE group (p<0.05). CR treatment also decreased the expression of iNOS, $p-I{\kappa}B$. Atopic dermatitis induced dermatological damage to skin, such as hyperplasia of epithelium, and capillary proliferation was significantly reduced by CR administration. CR effectively inhibited the thinning of the skin barrier and inflammatory responses in atopic dermatitis-induced mice. In particular, it showed anti-inflammatory effects by reducing the expression of IL-4 and IL-13, Th2 cell cytokines, which play a crucial role in development of atopic dermatitis. Therefore, CR can be a good candidate to ameliorate and treat atopic dermatitis.

한국인에서 NDFIP2 유전적 다형성과 천식의 상관 연구 (Association Study of NDFIP2 Genetic Polymorphism with Asthma in the Korean Population)

  • 최은혜;황다현
    • 대한임상검사과학회지
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    • 제53권3호
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    • pp.249-256
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    • 2021
  • 천식은 만성 염증성 기도 폐쇄 질환이다. 질병 발생 요인은 다양하며 특히, 유전적 요인과 환경적 요인이 천식 발병에 영향을 미치는 것으로 추정된다. MAPK (mitogen-activated protein kinase)경로는 Th1/Th2의 균형을 조절하며, 천식 발생에 중요한 역할을 하는 것으로 알려져 있다. 본 연구에서는 MAPK 경로를 조절하는 NDFIP2 유전자와 천식 발병과의 상관관계를 분석하였다. 193건의 천식 환자와 3,228건의 정상 대조군의 유전형 데이터를 사용하였다. 그 결과 NDFIP2 안에 있는 4개의 SNP이 천식과 유의한 상관관계와 높은 상대적 위험도를 보였다. 특히 NDFIP2의 rs2783122는 천식과 통계적으로 가장 유의한 연관성을 나타냈다(P-value=9.76×10-6, OR=1.67, 95% CI=1.33~2.10). NDFIP2 유전자에 대한 SNP imputation 결과 16개의 SNP가 추가 발견되었으며, 모두 유의한 상관 관계와 높은 상대적 위험도를 나타냈다. 유전자형 기반 mRNA 발현 분석을 통해 rs1408049가 minor allele을 가질 경우 유전자 발현이 증가됨을 알 수 있었다. 증가된 NDFIP2 발현은 MAPK 경로를 활성화시켜 천식 발병에 영향을 미칠 수 있다. 결론적으로 NDFIP2의 다형성은 천식 발병과 관련이 있으며, 이는 한국 인구의 천식 관리에 대한 새로운 지침을 제공할 수 있다.

Restorative effects of Rg3-enriched Korean Red Ginseng and Persicaria tinctoria extract on oxazolone-induced ulcerative colitis in mice

  • Ullah, H.M. Arif;Saba, Evelyn;Lee, Yuan Yee;Hong, Seung-Bok;Hyun, Sun-Hee;Kwak, Yi-Seong;Park, Chae-Kyu;Kim, Sung Dae;Rhee, Man Hee
    • Journal of Ginseng Research
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    • 제46권5호
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    • pp.628-635
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    • 2022
  • Background: Ulcerative colitis (UC) is the large intestine disease that results in chronic inflammation and ulcers in the colon. Rg3-enriched Korean Red Ginseng extract (Rg3-RGE) is known for its pharmacological activities. Persicaria tinctoria (PT) is also used in the treatment of various inflammatory diseases. The aim of this study is to investigate the attenuating effects of Rg3-RGE with PT on oxazolone (OXA)-induced UC in mice. Methods: A total of six groups of mice including control group, OXA (as model group, 1.5%) group, sulfasalazine (75 mg/kg) group, Rg3-RGE (20 mg/kg) group, PT (300 mg/kg) group, and Rg3-RGE (10 mg/kg) with PT (150 mg/kg) group. Data on the colon length, body weight, disease activity index (DAI), histological changes, nitric oxide (NO) assay, Real-time PCR of inflammatory factors, ELISA of inflammatory factors, Western blot, and flow cytometry analysis were obtained. Results: Overall, the combination treatment of Rg3-RGE and PT significantly improved the colon length and body weight and decreased the DAI in mice compared with the treatment with OXA. Additionally, the histological injury was also reduced by the combination treatment. Moreover, the NO production level and inflammatory mediators and cytokines were significantly downregulated in the Rg3-RGE with the PT group compared with the model group. Also, NLR family pyrin domain containing 3 (NLRP3) inflammasome and nuclear factor kappa B (NF-𝛋B) were suppressed in the combination treatment group compared with the OXA group. Furthermore, the number of immune cell subtypes of CD4+ T-helper cells, CD19+ B-cells, and CD4+ and CD25+ regulatory T-cells (Tregs) was improved in the Rg3-RGE with the PT group compared with the OXA group. Conclusion: Overall, the mixture of Rg3-RGE and PT is an effective therapeutic treatment for UC.

재난약자 대피 도움장치 활용을 위한 화재 피난 시뮬레이션 분석 연구 (A Study on Fire and Evacuation simulation analysis for use of Disaster Vulnerable Personal Evacuation Device)

  • 최두찬;황현수;고민혁;이시유
    • 한국재난정보학회 논문집
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    • 제16권4호
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    • pp.824-831
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    • 2020
  • 연구목적: 화재 발생 시 요양병원은 재실자 특성, 건물의 취약요소 등으로 인해 피난하는데 상당한 제약이 따르며, 요양병원 내 환자를 대피시킬 수 있는 제품 개발을 하여 피난의 효율을 높이고자 한다. 인공지능 기반 자율주행형 재난약자 대피 도움장치를 개발하였고, 효율적으로 사용하기 위해 화재 및 피난 시뮬레이션에 활용하였으며, 환자 대피를 돕는 조력자에 대한 분석을 연구하고자 한다. 연구방법: 화재 시뮬레이션을 통해 시간 별 연기 성상 분석을 하였으며, 최종적으로 주간 야간에 따른 조력자수를 입력하고, 피난시뮬레이션을 활용하여 피난 완료 시간을 비교 분석하였다. 연구결과: 재난약자 대피 도움장치를 활용하였을 경우 대피 시간은 20%이상 단축이 되는 것을 알 수 있었으며, 주간 대비 야간의 화재 발생 시 대피 시간은 70% 가까이 늦어짐을 알 수 있었다. 결론: 수평·수직 대피 도움장치를 실제 화재 상황에서 효율적으로 활용할 경우 상황에 맞는 전략이 필요할 것으로 판단된다. 추후 화재 상황별 시나리오에 따른 대피 매뉴얼을 개발함으로써, 수평 대피 도움장치 및 수직 대피 도움장치에 대한 활용에 대한 내용을 바탕으로 피난의 효율성이 증대될 것으로 기대된다.

전통적인 한방 처방 경옥고의 면역 증강 효과 (Immune-enhancing effects of a traditional herbal prescription, Kyung-Ok-Ko)

  • 노성수;이원화;김경민;나민균;배종섭
    • 대한본초학회지
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    • 제34권2호
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    • pp.41-47
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    • 2019
  • Objectives : A traditional herbal prescription, Kyung-Ok-Ko (KOK), has long been used in oriental medicine as an invigorant for age-related diseases, such as amnesia and stroke. However, the beneficial value of KOK for immune responses is largely unknown. Based on the above mentioned effects of KOK, other previous reports, and its use in traditional medicine, we hypothesized that KOK displays beneficial effects against methotrexate (MTX)-induced immune suppression. Methods : We investigated the effects of KOK (0.6 g/kg/day, oral (p.o.)) on deteriorated immunity caused by MTX (2 mg/kg/day, p.o.) in an immune suppression mouse model. MTX was fed to mice once a day for 7 days. After the immune responses of the mice deteriorated by MTX treatment, KOK in water was fed to the mice once a day for 14 days. We then measured the expression levels of various cytokines, such as T helper cell (Th1, Th2) cytokines, and the number of immune cells, such as spleen T cells, B cells, and macrophages. Results : The data showed that MTX decreased Th1 profiles (interferon $(IFN)-{\gamma}$, interleukin (IL)-2, IL-12) and the number of immune cells, and increased Th2 profiles (IL-4, IL-5, IL-13), which were normalized significantly by post-administration of KOK. However, there was no significant difference in body-weight gain between MTX- and KOK-treated mice. Conclusion : These results indicate that KOK has immune-enhancing functions and reduces immunotoxicity of MTX, suggesting that supplementation with KOK will improve immune responses clinically and be useful for the prevention of immune-related diseases.

폴리에틸렌 미세플라스틱과 납의 복합노출에 따른 실험동물의 세포면역기능 조절 장애 평가 (Dysregulation of Cellular Immune Functions on Gastric Administration to Mixtures of Polyethlene Microplastics and Metallic Lead in Mice)

  • 이경우;김창열
    • 한국환경보건학회지
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    • 제49권1호
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    • pp.22-29
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    • 2023
  • Background: The existing research results on the combined toxicity of these pollutants using mammals, such as rodents, are insufficient, especially in relation to changes in the immune system. Objectives: This study aims at evaluating the cellular immune response to PE-MPs solely or when combined with Pb, which possess excellent adsorption capacity with PE-MPs and is commonly co-exposed in our daily lives. Methods: The study investigated the cellular immune function of 9-week ICR mice with 28 days exposure to PE-MPs (2 mg/mouse/day) and Pb (0.1 mM in distilled water) individually and in combination. PE-MPs were administered via gastric intubation while the lead intake was conducted via the oral drinking water route. Cellular immunity was evaluated by analyzing the production for TH1 cytokines namely, TNF-α and IFN-𝛾 and TH2 cytokines, IL-4 and IL-6 in culture supernatants from polyclonally activated splenic mononuclear cells ex vivo. Results: Both the PE-MPs only and the PE-MPs+Pb exposure group revealed an increased TH1 response with elevated TNF-α and IFN-𝛾 levels and downregulated TH2 response with low IL-4, and IL-6 production levels compared to the control group. Furthermore, an increased IFN-𝛾/IL-4 ratio was found in the PE-MPs only and PE-MPs+Pb exposure groups, which indicated the skewedness to TH1 response. Meanwhile, reduced blood hemoglobin levels and increased levels of IL-4, the dominant TH2 cytokine in the Pb-only exposure group, were observed. Conclusions: Our current findings on the predominance of TH1 immune response in the PE-MPs and PE-MPs+Pb groups suggest that PE-MPs could be responsible for the predominant induction of the cellular immune changes. This finding could be used as an important landmark in research related to TH1 predominance, such as autoimmune diseases. It suggests that additional research on immune modulation using longer exposure durations or the same exposure route is required to elucidate stronger findings.

지역사회 거주 중고령자의 미충족 돌봄요구와 관련요인 (Unmet Care Needs Among Community-dwelling Middle-aged and Older People in Korea)

  • 김수정;박연환;김홍수
    • 한국노년학
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    • 제31권2호
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    • pp.195-209
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    • 2011
  • 이 연구는 지역사회에 거주하고 있는 중고령자의 미충족 돌봄요구 현황을 파악하고 관련요인을 밝히고자 수행한 이차자료 분석연구로, 전국의 45세 이상 일반가구 거주자를 대표하는 표본(n=10,254)을 표집하여 조사한 2006년 제 1차 고령화연구패널을 이용하였다. 미충족 돌봄요구는 기본적 일상생활수행과 도구적 일상생활수행에 있어 다른 사람의 도움을 필요로 하는 상태임에도 돌봐줄 수 있는 사람이 없는 경우로 정의하였다. 모든 분석은 대상 인구집단을 대표하는 추정 통계치를 구하고자 가중치를 적용하여 수행하였다. 연구 결과, 중장년의 7.3%, 젊은 노인의 14.5%, 그리고 고령 노인의 41.8%가 돌봄을 필요로 하는 상태였으나, 이 중 각각 34%, 33%, 24%가 돌봄요구를 충족받고 있지 못한 것으로 나타났다. 미충족 돌봄요구 관련요인은 연령 그룹에 따라 달랐는데, 중장년에서는 교육과 소득수준이 미충족 돌봄요구와 음의 상관관계를 갖고 있었던 반면, 젊은 노인과 고령 노인층에서는 독거만이 음의 상관관계를 가진 요인이었다. 결론적으로, 우리나라 지역사회 거주 중고령자 중 상당수가 미충족 돌봄요구가 있으며, 그 관련요인은 생애주기에 따라 차이가 있었다. 연구결과는 지역 사회 거주 중고령자의 미충족 돌봄요구를 최소화할 수 있도록 지역사회기반 재가서비스의 확충이 시급하며, 특히 교육수준이 낮은 중장년과 독거노인 대상의 서비스 개발 및 보급이 중요함을 시사한다.