• 한국조리학회지
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• 제23권1호
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• pp.28-36
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• 2017

This study made macaroon with the addition of Helianthus tuberosus L. powder so as to increase the application of Helianthus tuberosus L. powder (HTP) to food products and look into the quality characteristics depending on an addition ratio. Helianthus tuberosus L. powder 0%, 0.5%, 1.0%, 1.5%, and 2.0% were added, respectively, to make macaroon. As Helianthus tuberosus L. powder was added more, the moisture content tended to decrease more significantly (p<0.001). As Helianthus tuberosus L. powder was added more, lightness and yellowness tended to reduce more significantly (p<0.001). As Helianthus tuberosus L. powder was added more, the content of total phenol compounds and DPPH radical scavenging activity were significantly increased (p<0.001). In terms of texture analysis, hardness, gumminess, cohesiveness and chewiness were significantly increased, as the Helianthus tuberosus L. powder was increased (p<0.05). In terms of sensory test, regarding taste, 0.5% HTP group had the highest points. And as color, flavor, and texture were improved, 1.5% HTP showed the most desirable result in terms of overall acceptability. Results, if Helianthus tuberosus L. powder is added for cooking macaroon, it seems to increase anti-oxidation function and develop functional macaroon with excellent sensory factors.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2001년도 춘계 학술대회지
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• pp.52-53
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• 2001

• 대한본초학회지
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• 제32권5호
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• pp.39-46
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• 2017

Objective : This study was designed to evaluate the hypoglycemic effects of Helianthus tuberosi Rhizoma extracts and its optimum Heat processing conditions Methods : We investigated the Salivary ${\alpha}$-amylase, pancreas ${\alpha}$-amylase and ${\alpha}$-glucosidase inhibitory activities of extracts from Steam Heated Helianthus tuberosi Rhizoma Ext. The inhibitory activities of a 50% EtOH extract of Steam Heated Helianthus tuberosi Rhizoma Ext against ${\alpha}$-glucosidases were evaluated in this study. Inhibiting these enzymes involved in the absorption of disaccharides significantly decreases the postprandial increase in blood glucose level after a mixed carbohydrate diet. Furthermore, the postprandial blood glucose lowering effect of Steam Heated Helianthus tuberosi Rhizoma Ext. was compared to a known type 2 diabetes drug(Acarbose(R)) in a mice model. Steam Heated Helianthus tuberosus L. Ext significantly reduced the blood glucose increase after glucose loading. Results : The results were confirmed by real-time PCR that after treated with Streptozotocin in L6 cells, induced expression of GLUT4, after the steamed Helianthus tuberosus L. Ext. treated, observed its expression was increased. Steam Heated Helianthus tuberosus L Ext treated 4 hours in L6 cells, cytotoxicity was measured in MTT assay. Its toxicity were 5.7%, 9% and 11.3% at the treatment concentration $12.5{\mu}g/m{\ell}$, $25{\mu}g/m{\ell}$, the $50{\mu}g/m{\ell}$ respectively. Conclusions : Overall, the results of this study indicate that Hypoglycemic effect of Helianthus tuberosi Rhizoma caused by the Steam heat treatment, the optimum Heat processing condition is steamming at $121^{\circ}C$ for 30 min, and it will provide the basis for developing a useful dietary supplement for controlling postprandial hyperglycemia.

• 한국작물학회지
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• 제46권4호
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• pp.309-316
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• 2001

Helianthus tuberosus has been known to inhibit the growth of weeds and other plants sharing its habitat. This study was conducted to identify the allelochemicals of Helianthus tuberosus which were extracted with water and solvents. Aqueous extracts of leaf, stem, root, tuber and tuber peel of Helianthus tuberosus except tuber did not show significant differences in phytotoxicity to alfalfa seedlings. It was considered that Helianthus tuberosus contained fewer or less potential water-soluble substances that were toxic to alfalfa. Methanol extract of leaves of Helianthus tuberosus was sequentially partitioned in increasing polarity with n-hexane, ethylacetate and n-butanol. Each extract had a yield of 148, 12, 15.7 and 9.5g, respectively. Inhibitory effects on germination of alfalfa seeds treated with four fractions were not significantly different. But the significant reductions on hypocotyl length were observed for all the solvent extracts. Among the four fractions, the ethylacetate fraction showed the most significant inhibition effect on bioassay with alfalfa. Further separation of the active ethylacetate fraction by open column chromatography led to the 25 subfractions. In bioassay of each sub-fraction with alfalfa seeds, sub-fraction No. 13 showed the most inhibitory effect on seedling growth. $^1$H NMR and gas chromatography-mass spectrometry analysis revealed that sub-fraction No. 13 was the mixture of straight-chain saturated fatty acids.

• 한국환경농학회지
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• 제39권3호
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• pp.188-196
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• 2020

BACKGROUND: In order to cultivate upland crops in reclaimed land, economically appropriate crops should be selected. Jerusalem artichoke (Helianthus tuberosus L.) is one of the primary sources for inulin in higher plants. The objective of this study was to evaluate and compare growth and inulin of H. tuberosus L. according to the cultivation region in Korea. METHODS AND RESULTS: 21 varieties of H. tuberosus L. were collected in Korea, and then 7 varieties of H. tuberosus L. were selected for this study. To compare growth and inulin, those varieties were cultivated in reclaimed land and upland soils. The growth of H. tuberosus L. significantly decreased when cultivated in reclaimed land. There was an increas at 17.8% in the content of white H. tuberosus L. tuber inulin in reclaimed land. CONCLUSION: The content of inulin in H. tuberosus L. tubers was found to be dependent on cultivation region and tuber color. There was a difference of the inulin content between the tubers with different colors in reclaimed land. Purple tubers of P20 were considered suitable varieties for cultivation in reclaimed land. White tubers of W1 and W8 were also considered suitable varieties for cultivation in reclaimed land.

• 생약학회지
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• 제50권2호
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• pp.96-101
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• 2019

Methanol extract of Helianthus tuberosus L. (Compositae) flower was investigated to research the anti-oxidative activity by using a Caenorhabditis elegans model system. Ethyl acetate soluble fraction of the methanol extract showed the most potent DPPH radical scavenging activity. The ethyl acetate fraction was measured on its activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance with reactive oxygen species (ROS) level in C. elegans. Furthermore, in order to verify if regulation of stress-response gene is responsible for the increased stress tolerance of C. elegans which treated by the ethyl acetate fraction, we checked SOD-3 expression using a transgenic strain. Consequently, the ethyl acetate fraction of H. tuberosus flower increased the catalase and SOD activities in a dose-dependent manner in C. elegans, reduced ROS accumulation dose-dependently. Besides, the ethyl acetate fraction-treated CF1553 worms showed higher SOD-3::GFP intensity than the control group.

• 한국미생물·생명공학회지
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• 제50권2호
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• pp.228-234
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• 2022

Helianthus tuberosus is perennial plant as Compositae family and is shown various physiological activities such as analgesic, antipyretic, anti-inflammatory, anti-fungal, anti-spasmodic, aperient, cholagogue, diuretic, spermatogenic, stomachic, and tonic effects. In this study, we investigated the antidiabetic and anti-inflammatory effects of pancake mixture powder (PM) supplemented with H. tuberosus (PMH) in rat skeletal muscle L6 cells and murine macrophage RAW 264.7 cells, respectively. PM and PMH inhibited in vitro α-glucosidase activity. Glucose consumption was increased by PM and PMH without cytotoxicity in rat myoblast L6 cells. Western blot analysis revealed that PM and PMH down-regulated glycogen synthase kinase (GSK)-3β activation in L6 cells. PM and PMH inhibited inflammatory mediator, nitric oxide (NO) production without cytotoxicity in LPS-induced RAW 264.7 cells. The anti-diabetic and anti-inflammatory effects of PMH was more stronger than those of PM. Anti-diabetic and anti-inflammatory effects of PMH would be due to functional characteristics of the supplemented H. tuberosus and the presence of garlic and onion used as ingredients of PM. Taken together, our results that addition of functional materials such as H. tuberosus in product has synergic effects and PMH is potential candidate for treatment of diabetes through inhibiting inflammation.

• 현장농수산연구지
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• 제19권1호
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• pp.61-73
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• 2017

본 실험은 토양 수분이 토양 환경을 개선하고 유기물이 높은 토양과 유기물이 낮은 토양에서의 돼지감자(Jerusalem artichoke) 관수 처리와 무처리 및 백색종과 자색종에서 모종과 구근의 정식 형태에 따른 수량차이를 확인 하였다. 1. 돼지감자의 유기물 함량이 높은 토양과 유기물 함량이 낮은 토양의 수량비교는 유기물 함량이 높은 토양에서 증가를 확인하였다. 관수처리와 무관수 처리사이의 비교수량에서 관수처리에서 증가를 확인하였다. 모종정식과 구근정식사이의 수량비교에서 모종정식 처리에서 수량증가를 확인한 유의성을 나타내었다. 백색종과 자색종의 수량비교에서 백색종에서 수량 증가를 확인하였다. 2. 유기물 함량이 높은 토양에서의 관수와 무관수 사이의 수량비교는 관수처리에서 높은 수량을 확인하였다. 모종과 구근의 비교수량은 모종정식에서 수량이 증가하였다. 백색종과 자색종 사이의 비교수량에서 백색종에서 수량이 증가하였다. 3. 유기물 함량이 낮은 토양 안에서의 관수처리와 무 처리사이의 비교수량은 관수 처리에서 수량이 증가하였다. 모종과 구근정식사이의 비교수량은 모종정식에서 수량이 증가한 유의성을 나타내었다. 백색종과 자색종 정식사이의 비교 수량은 백색종 정식에서 수량이 증가하였다.

• 생약학회지
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• 제43권1호
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• pp.6-9
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• 2012

The $CH_2Cl_2$ soluble part of the leaves of Helianthus tuberosus L. (Compositae) exhibited a potent cytotoxic activity against the cultured human tumor cell lines including A-549, SK-OV-3, SK-MEL-2, XF498 and HCT-15 in vitro. Bioassaydirected fractionation of the $CH_2Cl_2$ soluble part of this plant led to the isolation of four cytotoxic sesquiterpene lactones having ${\alpha}$-methylene-${\gamma}$-lactone ring in the molecule. On the basis of physical and spectral evidences, their structures were characterized as ${\Delta}^{4,15}$-isoatripliciolide tiglate (1), ${\Delta}^{4,15}$-isoatripliciolide methacrylate (2), budlein A isobutylate (3) and budlein A tiglate (4). The ${\Delta}^{4,15}$-isoatripliciolide tiglate (1) showed the most potent cytotoxic activity ($0.26{\mu}M<ED_{50}<2.16{\mu}M$) against all of the cell lines tested.

• 한국식품영양과학회지
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• 제39권1호
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• pp.31-35
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• 2010

본 실험에서는 hamster $\beta$-cell인 HIT-T15 cell을 이용하여 돼지감자추출물의 생리활성 및 기능을 검증하고자 하였다. 돼지감자추출물을 첨가한 NC(0 ${\muL/mL$), HT2(1.1 ${\muL/mL$), HT3(1.5 ${\muL/mL$)군과 inulin을 첨가한 NC(0 ${\muL/mL$), IN2(1.8 ${\muL/mL$), IN3(2.5 ${\muL/mL$)군으로 나누어 실험하였다. 세포 viability 측정한 결과 시료를 첨가하지 않은 군을 100%로 보았을 때 돼지감자추출물을 첨가한 HT3(1.5 ${\muL/mL$)군과 inulin을 첨가한 IN2(1.8 ${\muL/mL$), IN3(2.5 ${\muL/mL$) 군에서 세포생존율이 유의적으로 증가하였다(p<0.05). 시료처리 후 췌장 $\beta$-세포 파괴를 유도하지 않고 HIT-T15 cell의 cell culture supernatant를 이용하여 cytotoxicity를 측정한 결과 시료를 첨가하지 않은 NC(0${\muL/mL$)군에 비해 모든 군에서 cytotoxicity가 낮게 나타났다. Alloxan(4 mM)으로 $\beta$-세포 파괴를 유도하여 HIT-T15 cell에서 세포보호 효과를 측정한 결과 시료를 첨가하지 않은 NC(0 ${\muL/mL$)군에 비해 돼지감자추출물을 첨가한 HT2(1.1 ${\muL/mL$), HT3(1.5 ${\muL/mL$)군에서 세포생존율이 유의적으로 증가하였다(p<0.05). 또한 췌장 $\beta$-세포 파괴를 유도하여 HIT-T15 cell이 분비한 인슐린 분비능 및 세포 내 $NAD^+$/NADH 함량을 측정한 결과 시료를 첨가하지 않은 NC(0 ${\muL/mL$)군에 비해 돼지감자추출물을 첨가한 HT3(1.5 ${\muL/mL$)군에서 인슐린 분비량과 $NAD^+$/NADH 함량이 유의적으로 증가하였다(p<0.05). 이상의 연구 결과 돼지감자추출물은 HIT-T15 cell의 생존율을 높이고, 세포보호 효과를 가짐으로써 인슐린 분비능 정상화 및 $NAD^+$ 함량을 증가시켜 혈당 조절 및 당뇨에 긍정적 효과가 있을 것으로 사료된다.