The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

The Effects of steam heat processing of Helianthus tuberosi Rhizoma on Blood glucose lowering

국우(菊芋) 증자가 혈당강하작용에 미치는 영향

  • Kim, Jin-Woo (Dept. of Plant Resources, Gyeongnam National University of Science and Technology) ;
  • Ha, Mi-Ae (Department of Agronomy & Medicinal Plant Resources, Gyeongnam national University of Science and Technology) ;
  • Shin, Yong-Wook (Department of Agronomy & Medicinal Plant Resources, Gyeongnam national University of Science and Technology)
  • 김진우 (경남과학기술대학교 식물자원학과) ;
  • 하미애 (경남과학기술대학교 농학.한약자원학부) ;
  • 신용욱 (경남과학기술대학교 농학.한약자원학부)
  • Received : 2017.08.11
  • Accepted : 2017.09.15
  • Published : 2017.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective : This study was designed to evaluate the hypoglycemic effects of Helianthus tuberosi Rhizoma extracts and its optimum Heat processing conditions Methods : We investigated the Salivary ${\alpha}$-amylase, pancreas ${\alpha}$-amylase and ${\alpha}$-glucosidase inhibitory activities of extracts from Steam Heated Helianthus tuberosi Rhizoma Ext. The inhibitory activities of a 50% EtOH extract of Steam Heated Helianthus tuberosi Rhizoma Ext against ${\alpha}$-glucosidases were evaluated in this study. Inhibiting these enzymes involved in the absorption of disaccharides significantly decreases the postprandial increase in blood glucose level after a mixed carbohydrate diet. Furthermore, the postprandial blood glucose lowering effect of Steam Heated Helianthus tuberosi Rhizoma Ext. was compared to a known type 2 diabetes drug(Acarbose(R)) in a mice model. Steam Heated Helianthus tuberosus L. Ext significantly reduced the blood glucose increase after glucose loading. Results : The results were confirmed by real-time PCR that after treated with Streptozotocin in L6 cells, induced expression of GLUT4, after the steamed Helianthus tuberosus L. Ext. treated, observed its expression was increased. Steam Heated Helianthus tuberosus L Ext treated 4 hours in L6 cells, cytotoxicity was measured in MTT assay. Its toxicity were 5.7%, 9% and 11.3% at the treatment concentration $12.5{\mu}g/m{\ell}$, $25{\mu}g/m{\ell}$, the $50{\mu}g/m{\ell}$ respectively. Conclusions : Overall, the results of this study indicate that Hypoglycemic effect of Helianthus tuberosi Rhizoma caused by the Steam heat treatment, the optimum Heat processing condition is steamming at $121^{\circ}C$ for 30 min, and it will provide the basis for developing a useful dietary supplement for controlling postprandial hyperglycemia.

Keywords

References

  1. Kang KK,Choi SC, Kim JS, Kim GC, Kim KM. Physiochemical Characteristics of Raw and Dried Jerusalem Artichoke Jangachi. J East Asian Soc Dietary Life. 2015 ; 25(5): 887-892. https://doi.org/10.17495/easdl.2015.10.25.5.887
  2. Shin SH, Kwon SJ, Jo HJ, Go DH, Han JJ. Extraction and analysis of inulin from Jerusalem artichoke. Food Science and industry. 2012 ; 4 : 50-58.
  3. Carabin IG, Flamm WG. Evaluation of safety of inulin and oligofructose as dietary fiber. Regul Toxicol Pharmacol. 1999 ; 30(3): 268-282. https://doi.org/10.1006/rtph.1999.1349
  4. Jeong HJ, Kim JS, Sa YJ, Kim MO, Yang JF, Kim MJ. Antioxidant Activity and ${\alpha}$-Glucosidase Inhibitory Effect of Jerusalem Artichoke (Helianthus tuberosus ) Methanol Extracts by Heat Treatment Conditions. Korean J. Medicinal Crop Sci.2011 ; 19(4) : 257-263. https://doi.org/10.7783/KJMCS.2011.19.4.257
  5. Kim JL, Bae CR, CHa YS.Helianthus tuberosus Extract Has Anti-Diabetes Effects in HIT-T15 Cell. J Korean Soc Food Sci Nutr. 2010 ; 39(1): 31-35. https://doi.org/10.3746/jkfn.2010.39.1.031
  6. Jin Y, Kim YJ, Jeon JN,Wang C,Min JW, Jung SY, Yang DC. Changes of Ginsenosides and Physiochemical Properties in Ginseng by New 9 Repetitive Steaming and Drying Process. Korean J. Plant Res. 2012 ; 25(4) : 473-481. https://doi.org/10.7732/kjpr.2012.25.4.473
  7. Lee MY, Jung SM, Lee GW. Monitoring on Benzo(a) pyrene Content in Oriental medicine. Journal of Digital Convergence. 2012 ; 10(7) : 201-206. https://doi.org/10.14400/JDPM.2012.10.7.201
  8. Blois MS. Antioxidant determination by the use of a stable free radical. Nature. 1958 ; 181 : 1199-1200. https://doi.org/10.1038/1811199a0
  9. Shin YW. Exploring the quality standard of Phellinus spp through ${\beta}$-glucan content and sensory evaluation. Kor. J. Herbol. 2017 : 32(4): 47-52 https://doi.org/10.6116/kjh.2017.32.1.47.
  10. Carmichael J, Degraff WG, Gadzar AF. Evaluation of a tetrazolium based semiautomated colorimetric assay : Assessment of chemosensitivity testing. Cancer Res. 1987 ; 477 : 936-942.
  11. Choi MA. Attenuation of insulin resistance using steamed Polygonatum odoratum var pluriflorum extract in rat skeletal muscle cells L6 myoblast. Kor. J. Herbol. 2016 ; 31(1) : 1-5. https://doi.org/10.6116/kjh.2016.31.1.1.
  12. Lee SY, Park SL, Nam YD, Yi SH, Lim SI. Antidiabetic Effects of Fermented Green Tea in KK-Ay Diabetic Mice. Korean J Food Sci Technol. 2013 ; 45(4) : 488-94. https://doi.org/10.9721/KJFST.2013.45.4.488
  13. Kahn SE. Clinical review 135: The importance of beta-cell failure in the development and progression of type 2 diabetes. Journal of Clinical Endocrinology and Metabolism. 2001 ; 86(9) : 4047-4058. https://doi.org/10.1210/jc.86.9.4047
  14. Ha KW, Kim DJ. Current status of managing diabetes mellitus in Korea. Korean J Intern Med. 2016 ; 31(5): 845-850. https://doi.org/10.3904/kjim.2016.253
  15. Woods SC, Seeley RJ, Rushing PA, D'Alessio D,Tso P. A controlled high-fat diet induces an obese syndrome in rats. J Nutr. 2003 ; 133 (4) : 1081-7. https://doi.org/10.1093/jn/133.4.1081
  16. Ham IH,Jeong ES, Lee BH, Choi HY. The Study on Anti-hypertensive and Anti-diabetic Effect of Mori Ramulus. Kor. J. Herbology 2008 ; 23 (2) : 203-212.
  17. Kwon TO, Choi JW, Lee HS, CHAnti-Diabetic Effects of Mori Folium Extract on High-Fat Diet and Streptozotocin-Induced Type II Diabetes Mellitus in Mice. Kor. J. Herbology 2015 ; 30(1) : 1-9. https://doi.org/10.6116/KJH.2015.30.1.1.
  18. Oh TWm Park YK. Effect of the root extract of Pueraria thunbergiana Bentham on high fat/high sucrose diet and single low-dose streptozotocininduced diabetic mice. Kor. J. Herbology 2014 ; 29(5) : 75-81. https://doi.org/10.6116/kjh.2014.29.5.75
  19. Han YK,Park YK. The comparisons of Lycii Radicis Cortex and Corni Fructus water extract effects on streptozotocin-induced diabetes in rats.Kor. J. Herbology 2013 ; 28(6) : 71-77. https://doi.org/10.6116/KJH.2013.28.6.71
  20. Lee YJ, Lee YJ,Yoon JJ,Lee SM,Kim HY,Shin SH, Kang DG, Lee HS. Anti-diabetic and Anti- Inflammatory Effects of Water Extract of Ligustrum japonicum Leaves in db/db Mouse. Kor. J. Herbology 2012 ; 27(6) : 107-114. https://doi.org/10.6116/kjh.2012.27.6.107
  21. Kim WL,Kim CS,Lee HY,Lee HR, Kim EY, Yoon MC, Shin SS. Mountain cultivated ginseng water boiled extract decreases blood glucose level and improves lipid metabolism in male db/db mice.Kor. J. Herbology 2012 ; 27(2) : 69-75. https://doi.org/10.6116/kjh.2012.27.2.69