• 대기
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• 제25권2호
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• pp.193-203
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• 2015

A large aperture scintillometer (LAS) was deployed with an optical path length of 2.1 km to estimate turbulent sensible heat flux (${\mathcal{Q}}_H$) over a highly heterogeneous urban area. Scintillation measurements were conducted during cold season in November and December 2013, and the daytime data of 14 days were used in the analysis after quality control processes. The LAS-derived ${\mathcal{Q}}_H$ show reasonable temporal variation ranging $20{\sim}160W\;m^{-2}$ in unstable atmospheric conditions, and well compare with the measured net radiation. The LAS footprint analysis suggests that ${\mathcal{Q}}_H$ can be relatively high when the newly built-up urban area has high source contribution of the turbulent flux in the study area ('northwesterly winds'). Sensitivity tests show that the LAS-derived ${\mathcal{Q}}_H$ are highly sensitive to non-dimensional similarity function for temperature structure function parameter, but relatively less sensitive to surface aerodynamic parameters and meteorological variables (temperature and wind speed). A lower Bowen ratio also has a significant influence on the flux estimation. Overall uncertainty of the estimated daytime ${\mathcal{Q}}_H$ is expected within about 20% at an upper limit for the analysis data. It is also found that stable atmospheric conditions can be poorly determined when the scintillometry technique is applied over the highly heterogeneous urban area.

• 한국대기환경학회지
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• 제16권4호
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• pp.339-350
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• 2000

The three-dimensional new corrdinate system over a single hill double hills and complex terrain with a single hill and a rectangular obstacle was generated using a body-fitted coordinate system. Control of the coordinate line distribution in the field was executed by generalizing the elliptic generating system to Poisson equation. ▽2ξ=P. The new coordinate system was well fitted to the surface boundary of single hill and double hills. But in the case of complex terrain with hill and rectangular obstacle there was smoothing tendency around the rectangular obstacle. In order to show the validity of the body-fitted coordinate system the heat diffusion equation was transformed and the temperature distribution was calculated over the various terrain. The results showed the temperature distribution was very symmetrical and stable around hills and obstacle. As a result the couple of a body-fitted coordinate system and the heat diffusion equation were executed successfully. Wind field over complex terrain with hill and rectangular obstacle which represent urban area was simulated stably in body-fitted coordinate system. The qualitative result show the enhancement of wind speed at the upwind direction of a hill and a rectangular obstacle and the recirculation zone at the downwind direction.

• Journal of Microbiology and Biotechnology
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• 제24권8호
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• pp.1118-1122
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• 2014

The present study shows that DR1114 (Hsp20), a small heat shock protein of the radiation-resistant bacterium Deinococcus radiodurans, enhances tolerance to hydrogen peroxide ($H_2O_2$) stress when expressed in Escherichia coli. A protein profile comparison showed that E. coli cells overexpressing D. radiodurans Hsp20 (EC-pHsp20) activated the redox state proteins, thus maintaining redox homeostasis. The cells also showed increased expression of pseudouridine (psi) synthases, which are important to the stability and proper functioning of structural RNA molecules. We found that the D. radiodurans mutant strain, which lacks a psi synthase (DR0896), was more sensitive to $H_2O_2$ stress than wild type. These suggest that an increased expression of proteins involved in the control of redox state homeostasis along with more stable ribosomal function may explain the improved tolerance of EC-pHsp20 to $H_2O_2$ stress.

• 한국농공학회논문집
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• 제50권2호
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• pp.55-63
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• 2008

We investigated characteristics of temperature variation in urban and suburban areas(e.g., paddy field, upland, park, residential area) and urban heat island(UHI) during winter(December 2005 to February 2006). The daily maximum air temperature was not significantly different between suburban and urban areas, whereas the daily minimum air temperatures were significantly lower in the suburban areas than that in the residential area. The wind speed in the urban park(0.3 m/s) was much lower than that in the paddy fields(2.3 m/s), likely due to an urban canopy layer formed by high buildings. The UHI intensity was represented by differences in daily minimum temperatures between urban residential and paddy field areas. The UHI intensity($4.1^{\circ}C$) in winter was larger than that($2.6^{\circ}C$) in summer. This may be because a stable boundary layer develops in the winter, and thereby this inhibits diffusion of heat from surface.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.167-170
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• 2000

B. Stearothermophilus 단백질 분해 효소는 2가 금속 이온인 $Ca^{2+}$에 의하여 내열성이 향상되며 $75^{\circ}C$에서 최대 역가를 나타내었다. 2mM $Ca^{2+}$를 첨가하여 B. Stearothermophilus를 이용한 호열 ${\cdot}$ 호기성 소화공정 운전할 경우 각각 최대량 대비 51%와 27%의 DOC 및 세포외단백질의 분해가 이루어지는 것을 확인하였다.

• 대한기계학회논문집B
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• 제23권10호
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• pp.1213-1222
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• 1999

A finite element method is developed for calculating the temperature and enthalpy distribution and accordingly the solid, liquid and mushy zone in a three-dimensional body subjected to any heat boundary conditions. The method concurrently consider both temperature and enthalpy for consideration of the latent heat effect, differently from other methods of using a special energy balance equation for solving a mushy zone. The developed brick element has eight nodes with one degree of freedom at each node. The numerical method and procedure are verified using the results of one and two dimensional analytic solutions and by other researchers. It is shown that the present method presents a consistent and stable results in either abrupt or ranged phase change problems. Moreover, the numerical results by the present method are hardly effected by the calculation time steps which otherwise are difficult to determine in most phase change problems. Finally, as a three-dimensional application, a T-shaped body of a phase change is presented and the temperature and enthalpy variation along the time are solved.

• 대한기계학회논문집B
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• 제35권2호
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• pp.215-220
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• 2011

산업용 보일러의 열효율을 높이고 체적을 줄이기 위하여 0.5 t/h 급 무연소실 보일러를 개발하였다. 별도의 연소실이 없이 증발관이 화염에 노출된 구조에서 화염 안정성을 확보하기 위하여 금속섬유버너를 채택하였다. 증발관은 관군의 형태로 버너 하류에 배치되고 하류에 설치된 증발관에는 휜을 설치하여 열교환 면적을 확보하였다. 무연소실 보일러는 관군에 대한 열전달 관계식으로부터 설계하였고 본 연구에서는 시제품에 대한 실험 및 보일러 내부의 유동 및 열전달에 대한 수치해석을 수행하여 관군에 대한 관계식을 적용한 설계와 비교하였다.

• 대한기계학회논문집B
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• 제26권5호
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• pp.750-756
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• 2002

The effect of equivalence ratio and velocity on the stability of flame in dump combustor was studied in an atmospheric pressure, laboratory scathe dump combustor operating natural gas. Traditionally, peak-to-peak pressure, fluctuation of the heat release rate and Rayleigh index were used to find and control the combustion instability. Cross correlation coefficients, Ci,j which is defined as the normalized value of the integration of the product of two of the mixer pressure, dump plane pressure and heat release rate, are introduced to see whether the flame is stable or not. Ci,j shows more sensitive to combustion status than Rayleigh index in steadily burning flame. Also, the result indicates that the amplitude of Ci,j between heat release and mixer pressure goes up before the flame at the rich de-stabilizing equivalence ratio near $\psi$=0.85. t means Ci:j at this case has a potential to detect the de-stablizing moment in prior to becoming unstable in dump combustor.

• 한국정밀공학회지
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• 제30권12호
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• pp.1265-1271
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• 2013

In this work, modeling and analysis of thermal effects laser drilling under water for ceramics were presented. Laser is a unique tool for machining ceramics due to the characteristic of non-contact material removal. However, ablation by a laser often induces a thermal effect on the material and an increased heat-affected-zone or deposition of debris can be observed on the machined parts. The underwater surrounding improved a heat transfer rate to cooling down the machined part and could prevent any deposition of debris near the machined surfaces and edges. The heat modeling was applied to obtain the temperature distributions as well as temperature gradients between the material and surroundings. The cooling effect of the underwater laser drilling was improved and a more stable temperature distribution was calculated. The actual laser drilling results of ceramic laser drilling were presented to verify the effects of underwater laser drilling.

• BMB Reports
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• 제31권2호
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• pp.130-134
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• 1998

An aldolase gene has been cloned from Methanococcus jannaschii. The coding region of the gene has been expressed in E. coli using a pET system to a level of 30% of total cellular proteins. The protein was purified to more than 95 % homogeneity by heat treatment and ion exchange chromatography. The protein performed an aldol condensation reaction with glyceraldehyde as substrate and dihydroxyacetone phosphate as a carboxyl donor. The protein was determined to be a type II aldolase which requires the $Zn^{2+}$ ion as a metal cofactor. This enzyme has a broad range of optimum pH (7-9) and temperature ($50-80^{\circ}C$). It shows strong stability against heat, chemical denaturants, as well as a high percentage' of organic solvents. The half-life of this enzyme at $85^{\circ}C$ is more than 24 h and it maintains more than 90% of aldolase activity in the presence of 6 M urea, 50% acetonitrile, or 15% isopropyl alcohol.