• Archives of Plastic Surgery
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• 제34권2호
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• pp.156-162
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• 2007

Purpose: In the previous in vitro studies the bone marrow stromal cells(BSCs) have shown the superior effect for wound healing activity than fibroblasts, which includes cell proliferation, type I collagen synthesis, and the production of bFGF, VEGF and TGF-${\beta}$ in chronic wound healing. The aim of this study is to compare the effects of BSCs and fibroblasts on wound healing activity in vivo, especially on collagen synthesis. Methods: The fibroblasts and BSCs were harvested from patients and cultured. The cultured cells were infiltrated into the pores of polyethylene discs. These discs were divided into three groups according to the mixed cells. In groups I, II and III the discs were loaded with no cells, fibroblasts and BSCs, respectively. Twelve discs per group(total 36 discs) were made for this study. After creating 6 pockets in the back of each rats, each discs was implanted into each pockets. At three time intervals from 1 to 3 weeks, the implanted discs were harvested for the histological and quantitative analysis. The amount of collagen produced was evaluated using ELISA. Statistical comparisons were made using the Mann-Whitney U-test. Results: There was great difference in the collagen synthesis among the three groups by the 1st and 2nd weeks. The BSC group showed highest collagen level, followed by fibroblast group and no cell group(p<0.05). The 3rd week specimens also showed greater collagen amount in BSC and fibroblast groups compared to those of no cell group(p<0.05). However, there was little difference between BSC and fibroblast groups. Conclusion: This result demonstrates that BSC has superior effect on stimulating wound healing than fibroblast, which is currently used for wound healing.

• 한국건설순환자원학회논문집
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• 제9권3호
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• pp.397-404
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• 2021

본 연구에서는 고상캡슐을 활용한 자기치유 표면보호재를 보수재료로 활용하기 위한 일환의 연구로써 고상캡슐이 표면보호재의 품질에 미치는 영향 및 장기재령의 치유 특성을 검토하였다. 고상캡슐 혼합에 따른 자기치유 표면보호재의 유변학적 특성 평가결과, 소성점도 및 항복응력, 테이블 플로우는 감소하는 경향이 나타났으며, 압축강도의 경우 고상캡슐 1%당 1 MPa이 비례적으로 감소하였다. 장기재령 치유특성을 평가한 결과, 재령 91일이 경과된 경우에도 고상캡슐이 보존되어 10% 혼합한 경우 치유재령 28일에 90% 수준의 치유율이 나타났으며, 치유재령 91일 경과후에는 고상캡슐 5%의 경우에도 90% 수준의 치유율이 나타났다.

• 한국구조물진단유지관리공학회 논문집
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• 제23권1호
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• pp.113-121
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• 2019

본 연구에서는 시멘트 복합재료와 직접 혼합 가능한 자기치유 마이크로 캡슐을 제조하였으며, 자기치유 마이크로 캡슐이 혼합된 시멘트 복합재료의 품질 및 균열 치유 성능 특성을 평가하였다. 종래의 경우 자기치유 캡슐 제조와 균열 치유 특성 평가에만 치중되어 평가되어 왔다. 따라서 자기치유 마이크로 캡슐은 시멘트 복합재료와 혼합시 시멘트 복합재료의 품질에 미치는 영향이 있기 때문에 이에 대한 검토를 수행하였다. 자기치유 마이크로 캡슐을 혼합한 시멘트 복합재료의 테이블 플로우 및 공기량 평가 결과 혼합율에 관계없이 테이블 플로우 및 공기량은 큰 영향이 없는 것으로 나타났다. 압축강도 및 쪼갬인장강도는 캡슐 혼합율이 증가할수록 강도가 감소하는 경향이 나타났다. Water Flow에 따른 균열 치유 특성 평가 결과 초기 투수량이 감소하는 결과가 나타났으며, 시간 경과에 따라 반응 생성물 발생하여 균열이 치유되는 것을 확인 할 수 있었다.

• Transactions on Electrical and Electronic Materials
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• 제11권5호
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• pp.226-229
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• 2010

Many methods exist that promote wound healing, including light therapy, which consists of light beams that assist the human body in treating and sterilizing wounds, as well as regenerating cells. Irradiation with specific wavelengths of either laser or LED light has been shown to induce beneficial proliferation of fibroblasts that, depending on the size of the wound, can be effective in promoting wound healing. The experiments in this study utilized 8 week old 250~300 g Male Sprague Dawley Rats (ILAR Code: NTacSam:SD) and included a non-irradiation group and a 525 nm green LED irradiation group (n of each group = 7). In experiments animals were allowed to rest for 24 hours after wounds had been excised, which was followed by non- irradiation or 525 nm green LED irradiation therapy one hour per day for 9 days. Immunohistochemical staining was conducted for cytokeratin in order to precisely measure the defect size. In addition, Masson's trichrome staining was utilized in order to compare levels of collagen between the 525 nm green LED irradiation group and the non-irradiation group. Animals exposed to 525 nm green LED irradiation (p<0.05) healed at a faster rate and had increased collagenosis compared with the non-irradiated control group. Thus, treatment with 525 nm green LED irradiation had a beneficial effect on wound healing and should be considered as a possible alternative to low power laser treatment.

• 한국약용작물학회지
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• 제22권6호
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• pp.457-462
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• 2014

Prostaglandin (PG) $E_2$ is an important mediator of skin wound healing without excessive scarring and gastric ulcer healing. However, $PGE_2$ has a short lifetime in vivo because it is metabolized rapidly by 15-hydroxyprostaglandin dehydrogenase (15-PGDH). Ethanol extract of Eriobotryae folium (EFEE) elevated intracellular and extracellular $PGE_2$ levels in HaCaT cells and inhibited 15-PGDH ($ED_{50}$ : $168.4{\mu}g/mL$) with relatively low cytotoxicity ($IC_{50}$ : $250.0{\mu}g/mL$). Real-time PCR analysis showed that mRNA expression of cyclooxygenase (COX)-1 and COX-2 enzymes were increased and prostaglandin transporter (PGT) was decreased in HaCaT cells by EFEE. Moreover, wound healing effect of EFEE ($168.4{\mu}g/mL$) was comparable to that of TGF-${\beta}1$ (300 pg/mL) as a positive control. These results demonstrate that EFEE may be valuable therapeutic materials for the treatment of $PGE_2$ level dependent diseases.

• Archives of Plastic Surgery
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• 제33권1호
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• pp.21-30
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• 2006

Alginate, a polymer of guluronic and mannuronic acid, is used as a scaffolding material in biomedical applications. The research was to produce highly-purified alginate from seaweeds and to evaluate the efficacy of alginate as dermal substrate. Our alginate purification method showed a production rate as high as 25%. The purified alginate contained little polyphenol contents and endotoxin, proteins. For study of wound healing, full thickness skin defects were made on the dorsal area of the animal models. And then alginate, fibroblast-growth-factor mixed alginate, alginate-collagen complex, vaseline gauze as control were applied on the wound, respectively, and were evaluated grossly and histopathologically. For biocompatibility test, alginate and alginate-collagen complex discs were implanted on the back of Sprague-Dawly rats. Four weeks after implantation, the animals were examined immunologically against alginate and collagen. Alginate and FGF-mixed alginate, alginate-collagen complex group showed statistically higher percentage of wound contraction and wound healing than control group(p<0.05). Alginate-collagen complex group and FGF-mixed alginate group showed statistically higher percentage of wound healing than alginate group. The experiment of biocompatibility and immunologic reaction against impanted alginate or collagen needs more investigation. Highly-purified alginate from seaweeds by our purification method, showed the effect of wound healing, and addition of FGF or collagen increases the alginate's wound healing effect. It shows the possibility of alginate as a dermal substrate.

• Restorative Dentistry and Endodontics
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• 제38권4호
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• pp.194-203
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• 2013

Objectives: To investigate the effect of enamel matrix derivative (EMD) on periodontal healing of replanted teeth in animal models. Materials and Methods: The authors searched MEDLINE, PubMed, EMBASE, Cochrane Library, Web of Knowledge and Scopus for articles published up to Oct 2012. Animal studies in which EMD was applied in transplanted or replanted teeth with adequate controls and histological data were considered. Normal periodontal healing or root resorption determined by histology after EMD was applied in replanted teeth with adequate controls was used as outcome measures. The following search strategy was used: ('Emdogain' OR 'enamel matrix proteins' OR 'enamel matrix derivative') AND ('avulsion' OR 'transplantion' OR 'autotransplantation' OR 'replantation'). Results: Six animal studies were included in the final review. There was great heterogeneity in study design among included studies. Two studies with similar study designs were identified and analyzed by a meta-analysis. The pooled estimates showed a significantly higher normal healing and surface resorption and significantly less inflammatory and replacement resorption in EMD-treated groups compared with non-EMD-treated groups. Conclusions: With the limitations of this systematic review, the use of EMD led to greater normal periodontal healing and surface root resorption and less inflammatory and replacement root resorption in the presence of periodontal ligaments. However, no definite conclusion could be drawn with regard to the effect of EMD on periodontal healing and root resorption when no periodontal ligaments exist.

• Archives of Plastic Surgery
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• 제34권4호
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• pp.426-431
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• 2007

Purpose: The aim of this study is to compare the effects of bone marrow stromal cells(BSCs) and fibroblasts on wound healing activity in vivo, especially on epithelization. Methods: The fibroblasts and BSCs were harvested from patients and cultured. Ten Spague-Dawley white rats were used. A 5 mm punches were made to excise skin and subcutaneous tissue in a round fashion at six sites on the back area of each rat. Four hundred thousand cells suspended in 0.05 ml fibrinogen were applied to the created wounds. The cells in group I, II, and III were no cells, fibroblasts and BSCs. The lengths of epithelial gap at the widest wound site were compared with autopsy specimens obtained on the 6th day after cell therapy under light microscope. Statistical comparisons were performed using the Mann-Whitney U-test, and the p value < 0.05 was considered statistically significant. Results: The best epithelization was also seen in the BSC group, followed by fibroblast and no cell groups.Conclusion: These results demonstrate that BSC has superior effect on stimulating wound healing than fibroblast, which is currently used for wound healing.

• Journal of Ginseng Research
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• 제47권5호
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• pp.654-661
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• 2023

Background: Ginseng has been used as a traditional medicine and functional cosmetic ingredients for many years. Recent studies have focused on the potential biological effects of the ginseng berry and its ingredients. (+)-Syringaresinol (SYR) is enriched in ginseng berry and its beneficial effects on the skin have been recently reported. However, little is known about the its effects on the wound healing process of skin. Methods: Here, we evaluated the skin wound healing effect of (+)-SYR using the human fibroblast Hs68 cell and ex vivo pig and human skin tissue model. Scratch wound test and hydrogen peroxide (HPO) induce chemical wound model were employed. Results: (+)-SYR promoted the migration and proliferation of Hs68 cells without significant cytotoxicity at the tested concentrations. Especially, in ex vivo pig and human skin tissue, HPO-induced chemical wound was recovered almost completely by (+)-SYR. In line with the finding in Hs68, the protein expression levels of TGF-β and PCNA, a proliferation marker were increased, demonstrating the beneficial effects of (+)-SYR on skin wound repair. Conclusion: Collectively, we demonstrated that (+)-SYR from ginseng berry, can enhance the wound healing effect by accelerating cell proliferation and skin regeneration, suggesting the potential utility of (+)-SYR for skin wound repair.

• 한국전통조경학회지
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• 제39권2호
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• pp.29-38
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• 2021

집중력회복 이론(ART)에 의하면 인간이 치유특질을 가진 경관을 경험하면 의도적인 집중력으로 유발된 정신적 피로감이 치유될 수 있다. ART에서는 다양한 경관이 치유특질을 가질 수 있다고 인정하고, 경관의 네 가지 치유특질은 지각강도가 클수록 치유효과가 강해진다고 설명하였다. 지금까지 치유경관에 대한 연구는 주로 자연경관과 도시경관을 대상으로 연구되어 왔다. 본 연구의 연구목적은 다음과 같다. 첫째, RCS를 이용하여 양동마을 경관을 대상으로 치유특질을 평가하고, 치유특질이 대상지경관의 방문 선호도에 미치는 영향을 조사하고자 한다. 둘째, 양동마을경관을 대상으로 경관이미지를 평가하여, 경관이미지가 경관의 치유특질에 미치는 영향을 조사하는 것이다. 본 연구의 대상지는 경주시 양동마을이며, 127명의 대학생이 설문조사에 참여하였다. 그리고 경관 이미지를 분석하기 위한 SD 16항목, 치유특질을 평가하기 위한 Laumann (2001)의 RCS 22항목을 측정도구로 사용하였다. 연구결과는 다음과 같이 요약한다. 첫째, SD법을 통해 양동마을의 경관 이미지를 분석하였으며, 양동마을 경관 이미지에 대한 요인분석 결과는 장소성 요인과 특이성 요인으로 도출되었다. 둘째, RCS의 신뢰성과 타당성을 입증하고 있으며, 요인분석 결과 세 가지 요인이 추출되었다. 셋째, 경관 이미지가 RCS의 세 가지 치유특질에 긍정적인 영향이 있는 것으로 나타났다. 넷째, 도출된 RCS의 세 가지 치유특질이 방문선호도에 긍정적인 영향을 미치는 것으로 나타났으며, 상대적인 방문선호도에 미치는 영향력의 순서는 매력·적합, 탈출감, 공간감으로 분석되었다. 앞으로의 연구에서는 RCS의 신뢰성을 재검증하기 위한 연구가 필요하다. 또한, 다양한 경관유형을 대상으로 경관 이미지를 분석하여 치유특질에 미치는 영향을 구체적으로 파악하는 것도 필요하다고 판단된다.