Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
권호 표지

Effect of Transplantation of Bone Marrow Stromal Cells and Dermal Fibroblasts on Collagen Synthesis

골수기질세포와 진피섬유모세포의 이식이 교원질 합성에 미치는 영향

  • Choi, Won Il (Department of Plastic Surgery, Korea University College of Medicine) ;
  • Han, Seung-Kyu (Department of Plastic Surgery, Korea University College of Medicine) ;
  • Lee, Byung Il (Department of Plastic Surgery, Korea University College of Medicine) ;
  • Kim, Woo Kyung (Department of Plastic Surgery, Korea University College of Medicine)
  • 최원일 (고려대학교 의과대학 성형외과학교실) ;
  • 한승규 (고려대학교 의과대학 성형외과학교실) ;
  • 이병일 (고려대학교 의과대학 성형외과학교실) ;
  • 김우경 (고려대학교 의과대학 성형외과학교실)
  • Received : 2006.10.19
  • Published : 2007.03.10
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: In the previous in vitro studies the bone marrow stromal cells(BSCs) have shown the superior effect for wound healing activity than fibroblasts, which includes cell proliferation, type I collagen synthesis, and the production of bFGF, VEGF and TGF-${\beta}$ in chronic wound healing. The aim of this study is to compare the effects of BSCs and fibroblasts on wound healing activity in vivo, especially on collagen synthesis. Methods: The fibroblasts and BSCs were harvested from patients and cultured. The cultured cells were infiltrated into the pores of polyethylene discs. These discs were divided into three groups according to the mixed cells. In groups I, II and III the discs were loaded with no cells, fibroblasts and BSCs, respectively. Twelve discs per group(total 36 discs) were made for this study. After creating 6 pockets in the back of each rats, each discs was implanted into each pockets. At three time intervals from 1 to 3 weeks, the implanted discs were harvested for the histological and quantitative analysis. The amount of collagen produced was evaluated using ELISA. Statistical comparisons were made using the Mann-Whitney U-test. Results: There was great difference in the collagen synthesis among the three groups by the 1st and 2nd weeks. The BSC group showed highest collagen level, followed by fibroblast group and no cell group(p<0.05). The 3rd week specimens also showed greater collagen amount in BSC and fibroblast groups compared to those of no cell group(p<0.05). However, there was little difference between BSC and fibroblast groups. Conclusion: This result demonstrates that BSC has superior effect on stimulating wound healing than fibroblast, which is currently used for wound healing.

Keywords

Acknowledgement

Supported by : 보건복지부

References

  1. Embil JM, Papp K, Sibbald G, Tousignant J, Smiell JM, Wong B, Lau CY: Recombinant human platelet-derived growth factor-BB(becaplermin) for healing chronic lower extremity diabetic ulcers: an open-label clinical evaluation of efficacy. Wound Repair Regen 8: 162, 2000
  2. Kano M, Masuda Y, Tominaga T, Hori T, Kitaichi T, Yoshizumi M, Kitagawa T: Collagen synthesis and collagenase activity of cryopreserved heart valves. J Thorae Cardiovase Surg 122: 706, 2001
  3. Tsang MW, Wong WK, Hung CS, Lai KM, Tang W, Cheung EY, Kam G, Leung L, Chan CW, Chu CM, Lam EK: Human epidermal growth factor enhances healing of diabetic foot ulcers. Diabetes Care 26: 1856, 2003 https://doi.org/10.2337/diacare.26.6.1856
  4. Kuroyanagi Y, Yamada N, Yamashita R, Uchinuma E: Tissue-engineered product: allogeneic cultured dermal substitute composed of spongy collagen with fibroblasts. Artif Organs 25: 180, 2001
  5. Gohari S, Gambia C, Healey M, Spaulding G, Gordon KB, Swan J, Cook B, West DP, Lapiere JC: Evaluation of tissue-engineered skin(human skin substitute) and secondary intention healing in the treatment of full thickness wounds after Mohs micrographic or excisional surgery. Dermatol Surg 28: 1107, 2002
  6. Pierce GF: Inflammation in nonhealing diabetic wounds; the space-time continuum does matter. Am J Pathol 159:399, 2001
  7. Conget PA, Minguell JJ: Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells. J Cell Physiol 181: 67, 1999
  8. Watanabe N, Woo SL, Papageorgiou C, Celechovsky C, Takai S: Fate of donor bone marrow cells in medial collateral ligament after simulated autologous transplantation. Microsc Res Tech 58: 39, 2002
  9. Quirici N, Soligo D, Bossolasco P, Servida F, Lumini C, Deliliers GL: Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factor receptor antibodies. Exp Hematol 30: 783, 2002
  10. Han SK, Yoon TH, Lee DG, Lee MA, Kim WK: Potential of human bone marrow stromal cells to accelerate wound healing in vitro. Ann Plast Surg 55: 414, 2005 https://doi.org/10.1097/01.sap.0000178809.01289.10
  11. Folkman J, Klagsbrun M: Angiogenic factors. Science 235:442, 1987
  12. Mansbridge JN, Liu K, Pinney RE, Patch R, Ratcliffe A, Naughton GK: Growth factors secreted by fibroblasts: role in healing diabetic foot ulcers. Diabetes Obes Metab 1:265, 1999
  13. Lerman OZ, Galiano RD, Armour M, Levine JP, Gurtner GC: Cellular dysfunction in the diabetic fibroblast; impairment in migration, vascular endothelial growth factor production, and response to hypoxia. Am J Pathol 162: 303, 2003 https://doi.org/10.1016/S0002-9440(10)63821-7
  14. Simmons PJ, Torok-Storb B: CD34 expression by stromal precursors in normal human adult bone marrow. Blood 78: 2848, 1991
  15. Han SK, Chun KW, Kye MS, Kim WK: The effect of transplantation of human mesenchymal stem cells and dermal fibroblasts on the angiogenesis in rats. J Korean Soc Plast Reconstr Surg 31: 539, 2004
  16. Conrad C, Huss R: Adult stem cell lines in regenerative medicine and reconstructive surgery. J Surg Res 124: 201, 2005 https://doi.org/10.1016/j.jss.2004.09.015