Securing a range of wheat resources is of particular importance with respect to wheat breeding, as it provides a broad genetic foundation. Although wheat breeders have used different wheat germplasms as material resources in current breeding systems, the traits of most germplasms collected from foreign countries differ from the unique traits that have evolved in the Korean environment. In this study, conducted over a 2-year period (2018 and 2019), we therefore evaluated the agricultural traits 1,967 wheat germplasms collected not only in Korea but also worldwide. During the period from sowing to February, the average temperature in 2019 was greater than 1℃, whereas from March to June, the average temperature was approximately 0.9℃ higher in 2018. Compared with the growth recorded in 2018, the stem length in 2019 increased by approximately 20 cm in 2019, and there were notable differences heading date and maturation between 2018 and 2019. In 2019, the heading dates of 973 and 713 wheat resources were earlier and later than those in 2018, respectively. Moreover, stem length was found to be highly correlated with the heading date and maturation. In Korea, where the rainy season and tine of rice transplantation overlap with the time of wheat harvest, early flowering time with high grain yield has been the most important selection target with respect to wheat breeding. We anticipate that the findings of this study will provide would use a foundation for the selection of elite materials and the development of resource core-sets for Korean wheat breeding programs.
Seung-Kyo Jeong;Dohyeong Gwon;Bae-Hyeon Lee;Jeong-Hwan Suh;Rahmatullah Jan;Jae-Ryoung Park;Taehun Ryu;Kyung-Min Kim
Journal of Life Science
/
v.34
no.8
/
pp.567-575
/
2024
New breeding techniques (NBT) recognize specific DNA sequences and remove, modify, or insert DNA at a desired location, and can be used to treat genetic diseases in humans or to improve the traits of livestock or crops. In this study, we conducted a comparative analysis of various agricultural traits and assessed the safety of gene transferability in third-generation genome-editing rice (OsCKq1-G3) with T and G nucleotide insertions developed using the CRISPR/Cas9 SDN-1 method, in comparison to its parental line (Oryza sativa L., cv Ilmi). The analyzed traits included heading date, culm length, panicle length, tiller number, yield, germination rate, viviparous germination rate, shattering, after wintering seed viability, the presence of toxins and allergens. The target trait, heading date, exhibited a high significant difference of approximately 5 days. Culm length, panicle length, tiller number, yield showed no significant differences compared to the parental line. No T-DNA bands indicating gene transfer were detected. In the third generation of genome-edited rice, the T-DNA was confirmed to be eliminated as successive generations advanced through self-pollination. Through the analysis of germination rate, viviparous germination rate, shattering, and after wintering viability, we confirmed that the genome-editing rice has no potential for weediness. The ORF and amino acid sequences of the genome-editing rice did not reveal any toxins and allergens. The results of this study can be utilized as important data for the environmental risk assessment of genome-editing rice.
To investigate the effect of seeding date, seeding rate, and sprouted seeds on seedling establishment, yield and its components of rice in barley /rice reley cropping as a minimum tillage, Gancheokbyeo rice seeds sowed by hand broadcast in various overlapped days befer barley harvest. The highest rice seedling stand was at sowed on the day just before barley harvest and l00kg per hectare of seeding rate with sprouted seeds. Also number of tiller and panicle showed the similar tendency. Delayed rice heading dates were found 2 days at l0-day overlap, 2 days at 5-day overlap, and 4 days at 0-day overlap before barley harvest compared with machine transplanting rice at just after barley harvest using l0-days old seedling. The culm length of broadcast rice shortened 2∼4cm compared with the transplanting rice. The rice plants lodged slightly at seeding rate l00kg per hectare and at sowed on the day of barley harvest. The rice panicle number increased with later date sowing and higher seeding rate. Number of spikelets per panicle and ripened grains decreased at higher seeding rates. Sprouted seeds and higher seeding rates showed higher rice yield at later sowing dates.
The yield of Unkwangbyeo transplanted on May 10 was 596kg/10a in 2010. The rice yield decreased with delaying transplanting date. The head rice rate was 91.9% when transplanted in June 10, and the rate decreased as the transplanting got earlier. In 2011, the yield increased by 5~8% when transplanted on June 10 because of the increase of Ripened grain ratio and 1,000 grain weight. The yield, when transplanted in the distance of $30{\times}13cm$ with 5 plants, was higher than the other treatments. The yield of Oraebyeo, transplanted on June 10, decreased by 6% compared with transplanting on May 25, while the yield, transplanted in the distance of $30{\times}13cm$ with 5 plants, increased by 5% because of the increase in the number/$m^2$ of panicle and grain. The head rice rates of the two varieties increased by 5~7% and the Toyo palatability appeared to get improved when transplanted on June 10 possibly because of the effect of low ripening temperature by 0.9~$1.6^{\circ}C$ with delaying heading date from 9 to 12 days.
In order to select the best varieties and cultivation methods for the production of domestic wheat for Hodugwaja(a walnut shaped confection), we carried out a 3-year experiment (2015~2017) to investigate the effect of different factors (crop variety, planting date, nitrogen fertilization) in a double cropping system (wheat then rice) on crop yields in the Cheonan area. Rice is the second crop in the system, and requires an accumulated temperature for 40 days of about $840{\sim}930^{\circ}C$ to ensure ripening. Transplanting dates for rice between June 29 and July 6 were suitable; transplanting on or after July 13 does not ensure ripening. The daily hours of sunshine ranged from 6.3 to 6.5 hours, which were slightly higher than the optimum of 6.0 to 6.1 hours. The higher the nitrogen fertilizer treatment, the higher culm length, and spike length of wheat. The yield of wheat per 10a tended to increase as the amount of nitrogen fertilizer increased. Wheat yields were highest for the Sooan variety, followed by Goso, followed by Keumgang. The number of days from transplanting to heading of rice were shortest for the Jopyeong variety followed by Unkwang, followed by Haedam. The yield of milled rice per 10a increased as the transplanting date was earlier and the transplanting date of June 9 showed the average yield of more than 500 kg in three varieties. From the results of the experiment, we recommend the Goso wheat variety and the Haedam rice variety for growing in a double cropping system under the climatic conditions of the Cheonan area.
Kim, Sang-Su;Baek, Nam-Hyun;Seok, Soon-Jong;Lee, Seon-Yong;Cho, Dong-Sam
KOREAN JOURNAL OF CROP SCIENCE
/
v.40
no.2
/
pp.212-220
/
1995
The optimum seeding date and the suitable seeding period for puddled drill seeding of rice at southern plain area were determined by using Sinunbongbyeo (early maturing variety), Hwaseongbyeo(medium maturing var.) and Dongjinbyeo (late medium maturing var.) at National Honam Agricultural Experiment Station from 1993 to 1994, Seedling stand was decreased significantly at seeding on April 25 in all varieties, Threshold seed-ing dates for stable ripening was estimated as June 10 in Sinunbongbyeo, May 31 in Hwaseongbyeo and May 25 in Dongjinbyeo, The 1,000 grain weight and the ripened grain rate were highest at seed-ing on May 10 or May 25 in all varieties, and decreased significantly at seeding on June 25 in Sinunbongbyeo and seeding after June 10 in Hwaseongbyeo and Dongjinbyeo, The optimum average air temperature for 40 days after heading was 22.6$^{\circ}C$ for highest ripening rate and 22.0$^{\circ}C$ for heaviest 1,000 grain weight. The optimum seeding date for highest yield was May 9 in Dongjinbyeo, May 13 in Hwaseongbyo and May 15 in Sinunbongbyeo.
Rice consumption in Korea has been decreasing as the eating habits of the Korean people have diversified with rapid economic growth. Recently, floury endosperm rice cultivars were developed to boost rice consumption and replace wheat flour consumption with rice flour, which is vulnerable to viviparity under wet weather during the grain-filling stage because of its loosely packed starch granule structures. To overcome this limitation, it is necessary to find a suitable rice transplanting date to produce high-quality rice flour by altering the heading ecology type and changing the cultivation time by region. We examined four floury endosperm rice cultivars (FERC) in the Cheongju (central plain area) and Boeun (mid-mountainous area) regions of Korea from 2017 to 2019. Of the FERCs, the mid-late maturing types (MMT) Seolgaeng (SG), Hangaru (HGR), and Shingil (SGL) exhibited high yield and yield components after transplanting May 30 in both regions; the early maturing type (EMT) Garumi 2 (GRM2) also exhibited high yield after transplanting June 20 in Cheongju. In addition, MMTs showed the same tendency as the characteristics shown in Cheongju when grown in the Boeun region, and EMT displayed high yield and yield components after transplanting June 10. The FERCs could easily present pre-harvest sprouting in the rainy season during the grain-filling stage after 20 days post-heading because the mean temperature and frequency of more three-day rainfalls have increased over the last 5 years from the previous annual averages. Viviparity of HGR and GRM2 decreased as the transplanting date was delayed, with decreases of 2.3%-4.6% in HGR and 11.9%-23.1% in GRM2 according to the region. SGL was generally resistant to viviparity because of the Tongil type. To minimize pre-harvest sprouting and produce high yield of rice flour in the Chungbuk province, the most suitable transplanting time was the end of May in MMT and the middle and end of June in EMT.
The effects of seeding and clipping termination dates, and seeding and fertilizer rates on forage and grain yields were evaluated in 1989 harvest year for dual production of forage and grain of naked and malting barleys in Cheju province. In the experiment I, 'Saessalbori' (naked barleY)1 and 'Doosan 22' (malting barley) were seeded on Sept. 21, Oct. 12 and Nov. 2, respectively. An unclipped(grain-only) treatment that was planted on Nov. 2 was included for check. In the experiment II, the two above cultivars were planted at seed rate of 14, 20 and 26 and kg/l0a. Fertilizer rates were 9-6-12, 17-13-8 and 22-16-11 (N-P$_2$O$\sub$5/-K$_2$O) kg/l0a. Seeding dates were Oct. 13 and Nov. 7 for clipped treatments and grain -only treatment(seeding rate: 14kg/l0a, N-P$_2$O$\sub$5/-K$_2$O=9-6-12 kg/l0a), respectively. All treatments in both experiments were harvested for grain yield. In Saessalbori, survival following forage removal was 100% regardless of seeding and clipping termination dates, and seeding and fertilizer rates. In Doosan 22, survival percenage decreased with increasing seeding and fertilizer rates when plants was clipped in January to Febuary. Forage yield of two cultivars increased with early planting and delays in clipping termination and tended to increase as seeding and fertilizer rates were increased. Crude ash and fat were not affected by seeding and fertilizer rates, and clipping date. Crude protein increased with an increase in fertilizer rate and decreased with delays in clipping date. N free extract tended to increase with delaying clipping date and decreased with increasing fertilizer rate. Earlier planting resulted in earlier heading and maturity. Clipping slightly delayed heading of Saessalbori and greatly delayed that of Doosan 22. Maturity of Doosan 22 was delayed 3 to 5 days by increased fertilization. Clipping shortened culm length more severely in Doosan 22 than in Saessalbori. The later the clipping termination, the shorter the culm length. The number of spikes per m$^2$ and the number of kernels per spike were not affected by clipping in Saessalbori while those of Doosan 22 were decreased with delays in clipping termination. Delaying forage harvest resulted In a reduction in grain yield. However, final clipping on Feb. 27 reduced grain yield of Saessalbori by only 6-11 % compared to the grain-only treatment. In Doosan 22, forage harvest after Dec. 28 resulted in 6 to 66% reduction of grain yield. The data indicate that grazing of naked and malting barleys until late Feb. and late Dec., respectively, might not reduce grain yields when planted on mid-Sept. to mid-Oct.
Kim, Meing-Jooung;Sung, Byung-Ryeol;Choi, Gi-Jun;Kim, Ki-Yong;Lim, Keun-Bal;Ji, Hee-Chung;Jung, Ki-Beak
Journal of The Korean Society of Grassland and Forage Science
/
v.28
no.1
/
pp.7-12
/
2008
This experiment was carried out to examine the winter hardiness, heading date, quality, and yields of Festulolium hybrids, Festuca pratensis and Lolium multiflorum, which were cultivated during September 2005 to July 2006 at Suwon and Namwon. The results are as follows; Perun, Boxer, and Fleurial showed stronger or same level of hardiness than Hwasan 101, which is one of the strongest hybrids in winter hardiness. Therefore, we did not detect any problem in winter hardiness when these hybrids were cultivated in Korea. These are turned out to be mid and late maturing hybrids. The average heading date of three hybrids were 24 to 27 May in Suwon and Namwon. These date are same or 3 days later than Hwasan 101. The average fresh yields of 3 hybrids were increased by $17{\sim}29%$ than that of Hwasan 101. And the dry matter yields of Perun, Boxer, and Fleurial were increased by 39%, 26%, and 29%, respectively, than that of Hwasan 101. We could not detect any difference in the average fresh and dry matter yields between region, however there were significant differences in hybrids(p<0.05). Crude protein contents of 3 hybrids were higher to $14.3{\sim}16.6%$ than that of Hwasan 101. The neutral detergent fiber and acid detergent fiber were decreased by 3.9 and 7.0%, respectively, comparing to Hwasan 101. Therefore these hybrids have turned out to be excellent feed quality hybrids.
Journal of The Korean Society of Grassland and Forage Science
/
v.14
no.3
/
pp.238-246
/
1994
This experiment was carried out to determine the effects of seeding date and fall harvest method on the growth characteristics, forage yield and quality of winter rye(Seca1e cereale L.) at the forage experimental field, College of Agriculture and Life Sciences, SNU, Suweon from 5 September 1992 to 21 May 1993. The experiment was arranged in a split plot design with three replications. Main plots consisted of three harvests, no defoliation, cutting and grazing. Sub-plots consisted of seeding dates, early(5 September), mid(25 September) and late(l0 October). The results obtained are summari7d as follows; I . A 3-day difference in the first heading of rye was observed between no defoliation and fall grazing, while there was a 6day difference between early and late seeding dates. Therefore, the effect of seeding dates on the fist heading date of rye was larger than that of fall harvest methods. 2. CP content of rye with fall grazing was slightly higher averaged 16.5% compared with both fall cutting and no defoliated 1ye(15.6%). Mean CP content of rye at the early, mid and late seeding dates was 14.2, 14.5 and 19.1 %, respectively. 3. ADF content of rye was slightly higher with both fall cutting(29.99) and no defoliation(29.5%) than that of fall grazing(28.0%). ADF content of rye at the early, mid and late seeding dates was 30.7, 29.1 and 27.7%, respectively. Effect of fall harvest methods and seeding dates on NDF content of rye were similar to the observations made on ADF. 4. RFV and IVDMD of rye were lower with both no defoliation and fall cutting than fall grazing. but those of rye slightly increased with seeding dates progressed. 5. Dry matter yield of rye was the highest of 6,779 kg/ha with fall cutting, while the lowest yield of 6,240 kg/ha was obtained from no defoliation But no significant difference was found among the harvest methods. Dry matter yield of rye was significantly declined with later seedings. It is concluded that the highest forage yield of rye be possible from two harvest systems of fall and spring with earlier seeding rather than from one harvest system of spring.
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