• Advances in pediatric surgery
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• 제7권2호
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• pp.105-111
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• 2001

To investigate the diagnostic accuracy and applicability of barium enema(BE) and rectal suction biopsy with acetyl cholinesterase(AChE) histochemistry in the diagnosis of neonatal Hirschsprung's disease(HD), we retrospectively reviewed the findings of BE and AChE staining in 96 neonates with suspected HD during a 10-year period from January 1991 to December 2000. Sixty-nine cases of HD(58 males and 11 females) and 27 cases of non-HD are included in this study. In regard to BE, HD was based on definite transitional zone, suspicious HD on reversed rectosigmoid index(RSI <1), and non-HD on normal RSI(RSI>1). The histochemical criterion used for the diagnosis of HD was that of Chow et al(1977), i.e., the presence of many coarse discrete cholinergic nerve fibers in the muscularis mucosae and in the immediately subjacent submucosa regardless of infiltration of cholinergic nerve fibers in the lamina propria. Of 66 neonates with HD who underwent BE, transitional zone was identified in 33 cases(50 %) and reversed RSI in 19 cases(21 %), microcolon in 4 cases and normal finding in 10 cases(15 %) while of 27 neonates with non-HD, there was normal finding in 16 cases and reversed RSI in 9 cases(41 %). Thus diagnostic accuracy based on transitional zone was 64 %. The positive predictive value of reversed RSI for the diagnosis of HD was 68 %. Of 42 neonates with HD who underwent AChE histochemistry, there were 41 AChE-positive reactions and one AChE-negative reaction in a neonate with total colonic aganglionosis, while of 27 cases of non-HD, there were one equivocal AChE-positive reaction and 26 AChE-negative reactions. Thus AChE histochemical study showed a 97 % diagnostic accuracy with a 98 % sensitivity and a 96 % specificity. In conclusion, we believe that BE is valuable as a first diagnostic step since about 80 % of neonates with HD show significant radiologic findings such as a transitional zone or reversed RSI. AChE histochemical study was a more reliable diagnos tic tool showing a 97 % diagnostic accuracy, and is part.

• 대한방사선치료학회지
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• 제30권1_2호
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• pp.129-138
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• 2018

목 적 : 임상적으로 많이 시행하는 치료부위에 고차원 치료기법 적용 시 선형가속기에 장착된 다엽콜리메이터(Multi leaf collimator, MLC)의 형태가 치료계획에 미치는 영향을 연구해 보고자 한다. 대상 및 방법 : 본원에서 척추암, 폐암 정위적체부방사선치료(Stereotactic body radiation therapy, SBRT)를 받은 환자와 전립선암, 폐암, 두경부암, 전골반암, 유방암의 용적변조회전치료(Volumetric modulated arc therapy, VMAT)를 시행한 환자들을 부위별로 10명씩 총 70명 선정하였다. Truebeam STx(Varian Medical system, Palo Alto, CA)의 high definition MLC(HD MLC)와 Vitalbeam(Varian Medical system, Palo Alto, CA)에 장착된 millenium MLC(M MLC)를 사용하였고, 치료계획은 Eclipse(Version 13.7, Varian Palo Alto USA, CA)를 이용하여 동일한 조건으로 환자마다 각각 2개씩 수립하였다. MLC의 형태에 따른 치료계획을 평가하기 위해 PTV coverage, conformity index(CI), homogeneity index(HI)를 비교하였고, 정상조직은 각 부위별로 임상에서 사용하는 평가 지표를 분석하였으며, 정상조직에서 저 선량 영역을 비교하기 위하여 $V_{30%}$을 평가하였다. 추가적으로 각 부위별로 치료표적의 길이 및 체적을 조사하였다. 결 과 : PTV coverage는 HD MLC를 적용한 폐암 SBRT에서 평균선량이 0.52 %, 척추암 SBRT에서 평균 선량과 최대선량이 각각 0.68 %, 1.13 %로 M MLC에 비해 감소하였다. CI와 HI는 척추암 SBRT 치료계획에서 HD MLC가 1.144, 1.079로 M MLC의 1.160, 1.092 보다 낮은 수치를 나타냈다(p<0.05). 결정장기의 선량 평가는 폐암 SBRT 치료계획에서 치료받는 쪽 폐의 평균선량이 HD MLC에서 1.48 %로 낮아졌다. 전립선암 VMAT은 HD MLC를 적용한 치료계획에서 방광의 $V_{30}$과 평균선량이 0.53 %, 1.42 %, 직장의 $V_{25}$와 평균선량이 0.97 %, 0.69 %로 감소하였다(p<0.05). 유방암 VMAT은 오히려 HD MLC를 사용한 치료계획에서 심장의 평균선량이 0.83 %로 높아졌다. 다른 치료부위 평가지표들은 두 MLC를 적용한 치료계획 사이에 큰 차이를 보이지 않았다(p>0.05). 결 론 : 본 연구 결과 SBRT나 전립선암 VMAT과 같이 치료표적의 길이가 짧고 크기가 작은 경우 HD MLC를 사용하는 것이 PTV coverage 및 정상조직 보호 효과 면에서 다소 이점이 있는 것으로 나타났으나, 그 외 상대적으로 길고, 큰 폐암, 두경부암, 전골반암, 유방암 VMAT에서는 MLC의 형태가 치료계획에 미치는 영향은 크지 않았다.

• Clinical and Experimental Pediatrics
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• 제63권7호
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• pp.251-258
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• 2020

Exposure to environmental factors can cause interstitial lung diseases (ILDs); however, such types of ILDs are rare. From 2007 to 2011, an ILD epidemic occurred in South Korea owing to inhalational exposure to toxic chemicals in humidifier disinfectants (HDs). HD-associated ILDs (HD-ILDs) are characterized by rapidly progressing respiratory failure with pulmonary fibrosis and a high mortality rate of 43.8%-58.0%. Although 18.1%-31.1% of the general population used HDs, only a small proportion of HD users were diagnosed with HD-ILDs. This finding suggests that investigation of the pathophysiologies underlying HD-ILDs is needed in addition to the identification of susceptibility to HD-ILDs. Further, there have been several concerns regarding the diverse health effects of exposure to toxic chemicals in HDs, including those that have not been identified, and long-term prognoses in terms of pulmonary function and residual pulmonary lesions observed on follow-up chest images. In this review, we summarize the clinical features, pathologic findings, and changes in radiologic findings over time in patients with HD-ILDs and the results of previous experimental research on the mechanisms underlying the effects of toxic chemicals in HDs. Studies are currently underway to identify the pathophysiologies of HD-ILDs and possible health effects of exposure to HDs along with the development of targeted therapeutic strategies. The experience of identification of HD-ILDs has encouraged stricter control of safe chemicals in everyday life.

• 한국지역사회생활과학회지
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• 제26권4호
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• pp.633-645
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• 2015

This study investigates the genotoxicity of crude antifungal compounds produced by Lactobacillus plantarum AF1 (L.plantarum AF1) and Lactobacillus plantarum HD1 (L. plantarum HD1) isolated from kimchi. The genetic toxicity of crude antifungal compounds was evaluated in bacterial reverse mutation in Salmonella and Escherichia spp., chromosome aberrations in Chinese hamster lung cells, and micronucleous formations in mice. In bacterial reversion assays with Salmonella Typhimurium TA98, TA100, TA1535, TA1537, and WP2uvrA, crude antifungal compounds did not increase the number of revertant colonies in both the absence and presence of the 59 metabolic activation system. In the chromosome aberration test with Chinese hamster lung cells, crude antifungal compounds showed no increase in the frequency of chromosome aberrations in the short-period test with/without the S9 mix or in the continuos test. In the in vivo mouse micronucleus assay, crude antifungal compounds showed no increase in the frequency of polychromatic erythrocytes with micronuclei. The results show that crude antifungal compounds produced by L. plantarum AF1 and L. plantarum HD1 did not induce any genotoxicity.

• Journal of Preventive Veterinary Medicine
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• 제42권4호
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• pp.133-142
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• 2018

This study investigated the characteristics of obesity induced by a high-fat diet (HD) over 13 weeks in Rhbdf2 gene knockout (KO) mice. Forty 7-week-old Rhbdf2 wild and KO mice were used and the mice were divided into 4 groups: Wild-ND (n=10, Rhbdf2 wild mice, normal diet (ND)), Wild-HD (n=10, Rhbdf2 wild mice, HD), KO-ND (n=10, Rhbdf2 KO mice, ND) and KO-HD (n=10, Rhbdf2 KO mice, HD). The relative epididymal fat weight in KO-HD was significantly increased compared with that in KO-ND (P<0.01). The relative liver and spleen weights in KO-HD were decreased compared with those in Wild-HD (p < 0.05) and KO-ND (p < 0.01). The mRNA expression of SOD1 in KO-ND was significantly reduced compared with that in Wild-ND (p < 0.05). In Wild-ND and HD, the mRNA expressions of $TNF-{\alpha}$ and IL-6 in epididymal fat were significantly increased compared with those in KO-ND and HD (p < 0.01). A significant increase of $TNF-{\alpha}$ and IL-6 mRNA expression was observed in KO-HD compared with KO-ND (p < 0.01). These results indicated that Rhbdf2 genes may regulate high fat diet-induced obesity damage by anti-inflammatory and anti-oxidative roles in fat tissue of mice.

• 생명과학회지
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• 제28권7호
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• pp.857-863
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• 2018

본 연구는 함초의 보충식이가 고지방식이 흰쥐의 혈청 및 조직의 중성지방 농도와 골격근 내 $PGC-1{\alpha}$와 $PPAR-{\gamma}$ 단백질 발현에 미치는 영향에 대하여 연구하였다. SD계 수컷 흰쥐를 대조군(CD, n=8), 고지방식이군(HD, n=8), 고지방식이+ 5% 함초 보충식이군(SD, n=8)으로 분류하여 8주간 사육하였다. 그 결과 SD군의 지방조직 중량은 HD군에 비해 약 25%정도 유의하게 감소한 반면 골격근의 중량은 SD군이 HD군에 비해 약 5%정도 유의하게 증가하였다(p<0.01). SD군의 혈청과 간장 내 중성지방은 HD군에 비해 약 20% 유의하게 감소하였다(p<0.05). SD군의 골격근 내 $PGC-1{\alpha}$ 와 $PPAR-{\gamma}$ 단백질 발현은 HD군에 비해 1.5배 유의하게 높게 나타났다(p<0.01). 이상의 결과로 부터 함초 보충은 골격근 내 $PGC-1{\alpha}$와 $PPAR-{\gamma}$ 단백질 발현의 증가를 통하여 지방감소 및 근육량 증가에 효과적이었음이 시사되었다.

• 패션비즈니스
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• 제10권1호
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• pp.41-57
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• 2006

The purpose of this study were to provide dress forms and men's jacket blocks based on the analysis of their somatotype. As the result of the previous research, based on 1290 males of 20 to 54 year-old, the shapes of adult male were 20 and each body shape was classified by size factor, height and chest girth and master size was selected considering appearance frequency. Somatotype YS(master size: height 170cm, chest girth 91cm) and HD1(master size: height 170cm, chest girth 94cm) were selected to develop dress forms and men's jacket block in the study. The procedure and results were follows; 1. The dress forms of somatotype YS and HD1 were provide base on means of 61 body measurements and cross sections of shoulder, chest, waist, hip of subjects belong to each somatotype. 2. New men's jacket blocks of somatotype YS and HD1 were developed based on the body surface extracted by draping and the result of comparative investigation on the conventional jacket patterns by wearing test. Also the drafting methods of new men's jacket blocks were provided. 3. Wearing test by the sensory evaluation showed that the developed jacket blocks were estimated more highly than existing patterns.

• 성인간호학회지
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• 제12권1호
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• pp.88-98
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• 2000

Hemodialysis(HD)-associated hypotension is a frequent complication, but it is difficult to manage. Until now, several maneuvers have been tried to prevent the HD-associated hypotension. Of these, the sodium content of dialysate was regarded as an important factor for maintaining blood pressure during HD. In this study, we evaluated the effect of hypertonic dialysate on blood pressure, interdialytic weight gain and the incidence of thirst. The study was done for 6 weeks successively with 3 different groups. Each patient was dialysed with 3 different dialysates for 2 weeks: Group I(Conventional HD: sodium concentration: 137 mEq/L), Group II(Hypertonic HD: 147 mEq/L) and Group III (Sequential HD: from 147 to 140 mEq/L). Hemodynamic parameters(blood pressure, pulse rate and ultrafiltration rate), biochemical parameters(hematocrits, blood urea nitrogen, creatinine, osmolality, sodium, potassium, chloride, fasting blood sugar) and complications (interdialytic weight gain & thirsty sensation) were compared among 3 groups. The results were as follows: 1. Decline of systolic blood pressure and diastolic blood pressure at the time of a 3 hour check during hemodialysis was lower in the Group II than Group I and III (p=0.002; p=0.012). and decline of diastolic blood pressure at the time of a 4 hour check during hemodialysis was lower in the Group II and III than Group I (p=0.04). 2. Incidence of hypotensive episodes during dialysis was significantly lower in Group II than group I (p=0.0287). 3. The ultrafiltration in Group III at the time of 1 hour, 2 hour and 3 hour check during hemodialysis was higher than that in Group I and II at the time of 1 hour, 2 hour and 3 hour check during hemodialysis respectively (p=0.0001; p=0.0001; p=0.0004). 4. Interdialytic weight gain was higher in Group I($3.1{\pm}0.8$) than Group I($2.8{\pm}0.8$) and III ($2.9{\pm}0.9$) (p=0.0422). 5. Hematologic and biochemical results were not significantly different among 3 Groups. 6. Frequency of thirst was different in Group I, II and III, $0.05{\pm}0.12$, $0.41{\pm}0.24$and $0.22{\pm}0.29$ respectively (p=0.0259). The results suggest that hypertonic HD was effective in preventing HD-associated hypotension but interdialytic weight gain and thirst sensations were increased as compared with a conventional method. In this situation, sequential HD seems to be an alternative method to minimizes the side effect of hypertonic HD.

• Tuberculosis and Respiratory Diseases
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• 제56권1호
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• pp.77-84
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• 2004

연구배경 : 혈액투석을 받고 있는 만성신부전 환자들에서 수분저류나 폐포 모세혈관의 투과성 증가에 의한 폐부종이 폐기능장애에 중요한 역할을 한다. 그러나, 현재까지 혈액투석을 받고 있는 만성신부전 환자들에서 혈액투석이 단기간에 폐기능에 미치는 영향이나 기관지확장제에 대한 반응을 연구는 없었다. 이 연구에서는 만성신부전으로 혈액투석을 받고 있는 환자들에서 혈액투석이 폐기능에 미치는 영향 및 체중변화와 기관지확장제에 대한 반응의 상호관련성을 알아보고자 하였다. 방 법 : 만성신부전으로 혈액투석을 받고 있는 30명의 환자들을 대상으로 혈액투석간 체중증가, 혈액투석 후 체중감소와 간편폐기능검사 결과에 근거한 $FEV_1$, FVC, $FEV_1/FVC$, 기관지확장제에 대한 반응을 분석하였다. 결 과 : 대상 환자 30명의 혈액투석 전 $FEV_1$, FVC 각각 예측치의 $89{\pm}22%$, $86{\pm}19%$였고 혈액투석 후 환자들의 $FEV_1$, FVC는 각각 예측치의 $100{\pm}23%$, $94{\pm}18%$였다. 혈액투석간 체중증가와 혈액투석 전기관지확장제에 대한 반응, 혈액투석 후 체중감소와 혈액투석 후 기관지확장제에 대한 반응 사이의 유의한 상관관계는 없었다. 혈액투석 후 기관지확장제에 대한 반응도는 혈액투석 전과 비교하여 유의한 차이는 없었다. 결 론 : 혈액투석 후 폐기능은 향상되지만 체중변화와 기관지확장제에 대한 반응도의 상호관련성은 없었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.24-24
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• 2022

This present study was to identify a novel candidate gene that contribute to the elevated α-linolenic acid (ALA, ω-3) concentration in PE2166 from mutagenesis of Pungsannamul. Major loci qALA5_1 and qALA5_2 were detected on chromosome 5 of soybean through quantitative trait loci mapping analyses of recombinant inbred lines. With next generation sequencing of parental lines and Pungsannamul, and recombinant analyses, a potential gene, Glyma. 05g221500 (HD) controlling elevated ALA concentration was identified. HD is a homeodomain-like transcriptional regulator that may regulate the expression level of microsomal ω-3 fatty acid desaturase (FAD3) genes responsible for the conversion of linoleic acid into ALA in the fatty acid biosynthetic pathway. In addition, we hypothesized that combination of mutant alleles, HD and either of microsomal delta-12 fatty acid desaturase 2-1 (FAD2-1\ could reduce the ω-6/ω-3 ratio. In populations where HD, and FAD2-1A and FAD2-1B genes were segregated, combination of a hd allele from PE2166 and either of the variant FAD2-1 alleles were sufficient to reduce the ω-6/ω-3 ratio in seeds.