Journal of Preventive Veterinary Medicine

The Korean Society of Preventive Veterinary Medicine (한국예방수의학회)
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Analysis of high-fat diet-induced inflammatory responses in Rhbdf2 knockout mice

  • Kim, Sung-Jun (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Nam, Ki-Hoan (Laboratory Animal Resource Center, Korea Research Institute of Bioscience & Biotechnology) ;
  • Park, Seul-Gi (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Byun, Young-Sub (Laboratory Animal Resource Center, Korea Research Institute of Bioscience & Biotechnology) ;
  • Kim, Eun-Kyoung (Laboratory Animal Resource Center, Korea Research Institute of Bioscience & Biotechnology) ;
  • Cho, Sang-Mi (Laboratory Animal Resource Center, Korea Research Institute of Bioscience & Biotechnology) ;
  • Kim, Ha-rim (Laboratory Animal Resource Center, Korea Research Institute of Bioscience & Biotechnology) ;
  • Kim, Hyoung-Chin (Laboratory Animal Resource Center, Korea Research Institute of Bioscience & Biotechnology) ;
  • Lee, Hu-Jang (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University) ;
  • Lee, Beom Jun (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • Received : 2018.09.05
  • Accepted : 2018.11.07
  • Published : 2018.12.31
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Abstract

This study investigated the characteristics of obesity induced by a high-fat diet (HD) over 13 weeks in Rhbdf2 gene knockout (KO) mice. Forty 7-week-old Rhbdf2 wild and KO mice were used and the mice were divided into 4 groups: Wild-ND (n=10, Rhbdf2 wild mice, normal diet (ND)), Wild-HD (n=10, Rhbdf2 wild mice, HD), KO-ND (n=10, Rhbdf2 KO mice, ND) and KO-HD (n=10, Rhbdf2 KO mice, HD). The relative epididymal fat weight in KO-HD was significantly increased compared with that in KO-ND (P<0.01). The relative liver and spleen weights in KO-HD were decreased compared with those in Wild-HD (p < 0.05) and KO-ND (p < 0.01). The mRNA expression of SOD1 in KO-ND was significantly reduced compared with that in Wild-ND (p < 0.05). In Wild-ND and HD, the mRNA expressions of $TNF-{\alpha}$ and IL-6 in epididymal fat were significantly increased compared with those in KO-ND and HD (p < 0.01). A significant increase of $TNF-{\alpha}$ and IL-6 mRNA expression was observed in KO-HD compared with KO-ND (p < 0.01). These results indicated that Rhbdf2 genes may regulate high fat diet-induced obesity damage by anti-inflammatory and anti-oxidative roles in fat tissue of mice.

Keywords

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