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http://dx.doi.org/10.7856/kjcls.2015.26.4.633

A Genotoxicological Safety Evaluation of Crude Antifungal Compounds Produced by Lactobacillus Plantarum AF1 and Lactobacillus Plantarum HD1  

Chang, Hae-Choon (Dept. of Food and Nutrition, Chosun University)
Koh, Sang-Bum (Korea Testing & Research Institute, Health Care Research Laboratory)
Lee, Jae-Joon (Dept. of Food and Nutrition, Chosun University)
Publication Information
The Korean Journal of Community Living Science / v.26, no.4, 2015 , pp. 633-645 More about this Journal
Abstract
This study investigates the genotoxicity of crude antifungal compounds produced by Lactobacillus plantarum AF1 (L.plantarum AF1) and Lactobacillus plantarum HD1 (L. plantarum HD1) isolated from kimchi. The genetic toxicity of crude antifungal compounds was evaluated in bacterial reverse mutation in Salmonella and Escherichia spp., chromosome aberrations in Chinese hamster lung cells, and micronucleous formations in mice. In bacterial reversion assays with Salmonella Typhimurium TA98, TA100, TA1535, TA1537, and WP2uvrA, crude antifungal compounds did not increase the number of revertant colonies in both the absence and presence of the 59 metabolic activation system. In the chromosome aberration test with Chinese hamster lung cells, crude antifungal compounds showed no increase in the frequency of chromosome aberrations in the short-period test with/without the S9 mix or in the continuos test. In the in vivo mouse micronucleus assay, crude antifungal compounds showed no increase in the frequency of polychromatic erythrocytes with micronuclei. The results show that crude antifungal compounds produced by L. plantarum AF1 and L. plantarum HD1 did not induce any genotoxicity.
Keywords
crude antifungal compounds; genotoxicity; reverse mutation; chromosomal aberration; micronucleus test;
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1 Alander M, Satokari R, Korpela R, Saxelin M, Vipponen-Salmela T, Von Wright A(1999) Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption. Appl Environ Microbiol 65(1), 351-354
2 Ames BN, McCann J, Yamasaki E(1975) Methods for detecting carcinogens and mutagens with Salmonella/ mammalian - microsome mutagenicity test. Mutation Res 31(6), 347-364   DOI
3 An SJ, Kim JY, Choi IS, Cho KK(2013) Insights into the roles of prebiotics and probiotics in the large intestine. J Life Sci 23(10), 1295-1303   DOI
4 Baek YJ(1993) Lactic acid bacteria and human health. Korean J Food Nutr 6(1), 53-65
5 Jung HK, Kim ER, Yae HS, Choi SJ, Jung YJ, Juhn SL(2000) Cholesterol-lowering effect of lactic acid bacteria and fermented milks as probiotic functional foods. Food Ind Nutr 5(2), 29-35
6 Kato I, Endo K, Yokokura T(1994) Effects of oral administration of Lactobacillus casei on antitumor responses induced by tumor resection in mice. Int J Immunopharmacol 16(1), 29-36   DOI
7 Kimmel SA, Roberts RF, Zieger GR(1998) Optimization of exopolysaccharide production by Lactobacillus delbrueckii ssp. bulgaricus RR grown in a semidefined medium. Appl Environ Microbiol 64(2), 659-664
8 Klaenhammer TR (1988) Bacteriocin of lactic acid bacteria. Biochim 70(3), 337-349   DOI
9 Lee H, Lee JJ, Chang HC, Lee MY(2012) Acute toxicity of Lactobacillus plantarum AF1 isolated from kimchi in mice. Korean J Food Preserv 19(2), 315-321   DOI
10 Lee JJ, Kim AR, Chang HC, Lee MY(2011) Repeateddose oral toxicity of Lactobacillus plantarum AF1 isolated from kimchi in rats. J Korean Soc Food Sci Nutr 41(5), 612-620   DOI
11 Lee SG, Han KS, Jeong SG, Oh MH, Jang AR, Kim DH, Bae IH, Ham JS (2010a) A study on the sensory characteristic of yogurt and antimicrobial activity of Lactobacillus plantarum LHC52 isolated form kimchi. Korean J Food Sci Ani Resour 30(2), 328-335   DOI
12 Lee SG, Lee YJ, Kim MK, Han KS, Jeong SG, Oh MH, Jang A, Kim DH, Bae IH, Ham JS(2010b) A study on the yogurt manufacture suitability and antimicrobial activity of Lactobacillus plantarum LHB55 isolated form kimchi. J Anim Sci Technol 52(2), 141-148   DOI
13 Lim SM, Im DS(2007) Bacterial effect of bacteriocin of Lactobacillus plantarum K11 isolated from dongchimi on Escherichia coil O157. J Food Hyg Saf 22(3), 151-158
14 OECD(2001a) OECD guideline testing of chemicals No. 471 Bacterial reverse mutation test.
15 Maron, DM, Ames BN(1983) Revised methods for the Salmonella mutagenicity test. Mutat Res 113(3-4), 173-215   DOI
16 Messens W, De Vuyst L(2002) Inhibitory substances produced by Lactobacillus isolated sourdoughs - a review. Int J Food Microbiol 72(1-2), 31-43   DOI
17 Ministry of Food and Drug Safety(2014) Guideline for toxicity tests of drugs and related materials notification. Chungbuk, MFDS
18 OECD(2001b) OECD guideline testing of chemicals No. 473 in vitro mammalian chromosome aberration test.
19 OECD(2001c) OECD guideline testing of chemicals No. 474 mammalian erythrocyte micronucleus test.
20 Rim KT, Kim SJ, Kim JG, Kim HY, Yang JS(2009) Bacterial reversion mutation (AMES) test of aluminium oxide, calcium oxide, and sodium tetraborate. J Korean Soc Environ Anal 12(3), 196-203
21 Ryu EH, Yang EJ, Woo ER, Chang HC(2014) Purification and characterization of antifungal compounds from Lactobacillus plantarum HD1 isolated from kimchi. Food Microbiol 41(1), 19-26   DOI
22 Seo J, Lee GS, Kim JE, Chung MJ(2010) Development of probiotic products and challengers. KSBB J 25(4), 303-310
23 Shida K, Makino K, Morishita A, Takamizawa K, Hachimura S, Ametani A, Sato T, Kumagai Y, Habu S, Kaminogawa S(1998) Lactobacillus casei inhibits antigen-induced IgE secretion through regulation of cytokine production in murine splenocyte culture. Int Arch Allergy Immnunol 115(4), 278-287   DOI
24 Yang EJ(2008) Application of kimchi lactic acid bacteria and Bacillus subtilis for development of novel antibiotics and edible vaccine. PhD Thesis, Chosun University. Korea
25 Song HP, Shin EH, Yun H, Cho C, Kim D(2009) Establishing the genotoxicological safety of gammairradiated egg white and yolk. Korean J Food Preserv 16(5), 782-788
26 Stiles ME(1996) Biopreservation by lactic acid bacteria. Antonie van Leewenhoek 70(2-4), 331-345   DOI
27 Tagg JR, Dajani AS, Wannamaker LW(1976) Bacteriocins of gram-positive bacteria. Bacteriol Rev 40(3), 722- 756
28 Yang EJ, Chang HC(2008) Antifungal activity of Lactobacillus plantarum isolated from Kimchi. Korea J Microbiol Biotechnol 36(4), 276-284
29 Drider D, Fimland G, Hechard Y, McMullen LH, Prevost H(2006) The continuing story of class II a bacteriocins. Microbiol Mol Biol Rev 70(2), 564-582   DOI
30 Dean BJ, Danford N(1984) Assays for the detection of chemically-induced chromosome damage in cultured mammalian cells. In: Mutagenicity testing - a practical approach, Venitt S & Parry JM(Editors), IRL Press Limited, Eynsham, England, pp187-232
31 Gupta R, Srivastava S(2014) Antifungal effect of antimicrobial peptide(AMPs LR14) derived from Lactobacillus plantarum strain LR/14 and their applications in prevention of grain spoilage. Food Microbiol 42(1) 1-7   DOI
32 Hayashi M(1991) The micronucleus test. Science Press Inc., Tokyo, Japan, pp 65-69
33 Hayashi M, Yoshimura I, Sofuni T, Ishidate M Jr(1989) A procedure for data analysis of the rodent micronucleus test involving a historical control. Environ Mol Mutagen 13(4), 347-356   DOI
34 Huang YH, Jung DW, Kang IJ(2014) Genotoxicity safety evaluation of imported oranges irradiated with ionizing energy. J Korean Soc Food Sci Nutr 43(6), 909-915   DOI
35 Internationally Chemical Hamonization(2012) Genotoxicity testing and data interpretation for pharmaceuticals intended for human use. International Conference on Harmonization, USA
36 Ishidate M Jr, Odashima S(1977) Chromosome test with 134 compounds on Chinese hamster cells in vitro-A screening for chemicals carcinogen. Mutat Res 48(3-4), 337-354   DOI
37 Ishidate M Jr, Sofuni T, Yoshikawa K(1981) Chromosomal aberration tests in vitro as a primary screening tool for environmental mutagens and/or carcinogens. Gann Monogr Cancer Res 27(1), 95-107