• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.1
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• pp.16-23
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• 1987

The cyprinid loach, Misgurnus mizolepis $G\ddot{u}nther$, a freshwater teleost, is a member of the family Cobitidae and is known to distribute in Korea and China. This fish is considered to be an excellent food fish in Korea. This report is a description of the gross morphology of the embryos and larvae up to the development of juvenile period of life. The eggs were obtained by injecting gonadotropin and fertilized by wet method in the laboratory on July 10 in 1986. The inseminated eggs were rinsed with tap water and incubated at $25^{\circ}C$. Live, freshly laid eggs were separative, demersal, adhesive, light yellow and average 1.1mm in diameter. The hatching took place in about yellow and average 1.1mm in diameter. The hatching took place in about 24 hours after fertilization. The newly hatched larvae were average 2.7 mm in total length possessing yolk sac and 35-36 myotomes. Ten hours after hatching, the prelarvae attained 3.5 mm in total length. The first heart pulse and melanophores at the side of the body were observed at this stage. Yolk sac was completely reserved at four days after hatching. The larvae that was cultured 24 days after hatching, 15.4-16.4 mm in total length, entered the juvenile period of life. All the un rays were already peresent; D. III 6, A. III, 5, P.10, V. 4-5, C.14.

• International Journal of Industrial Entomology and Biomaterials
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• v.6 no.2
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• pp.133-138
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• 2003

Five seed kernel namely, Neem (Azadirachta indica A. Juss.), Pongamia (Pongamia glabra L. Pierre), Tamarind (Tamarindus indica L.), Mahua (Madhuca indica Gmel.) and Shikakai (Acacia cancinna De.) were tested against hatching of eggs and larval mortality of Meloidogyne incognita causing root knot disease in mulberry along with Furadan (Carbofuran) and Bionema (a bioformulation developed from Verticillium chlamydosporium) for comparison. Results revealed that highest hush-up of hatching was observed in Neem (77.40%) and Pongamia (75.99%) seed kernel extracts at 100% concentration over the check. Similarly, highest larval mortality was observed in Neem and Pongamia by 76.00% and 74.50%, respectively at 100% concentration after 72 hrs of exposure period. Pot culture studies revealed that pre-application of seed kernel powders (20 days before inoculation of nematode) found to be more effective in controlling the root knot disease than post application. In pre application of seed kernel powders, maximum reduction of root knots was observed in case of Neem seed kernel powder (54.85%) followed by Pongamia (51.9%). Similar trend was also observed in reduction of egg masses/plant and nematode population ／250 cc soil. Rest of the seed kernel extracts was found to be less effective in suppression of hatching, enhancing the larval mortality and controlling the root knot disease. However, application of Furadan and Bionema tested for the comparison were found to be more effective than seed kernel powders. The generated information seems to be useful in developing an ecofriendly integrated approach for the control of root knot nematode disease in mulberry.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.4
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• pp.479-485
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• 2020

We evaluated the hatchability of fertilized eggs from six hybrid combinations of highly valued grouper species inhabiting temperate and warm waters, with the goal of establishing a novel hybrid with enhanced growth and viability during the culturing period in the temperate waters of Korea. Hybrid combinations with red-spotted grouper females exhibited high hatchability with high a fertilization and hatching rate of fertilized eggs and a low deformity rate of hatched larvae. Conversely, hybrid combinations with kelp grouper females had very low hatching rates and very high deformity rates; commercial production of seed from such crosses would be difficult without improving hatchability. The hatchabilities of convict grouper ♀×giant grouper ♂ and kelp grouper ♀×red-spotted grouper ♂ were lower than those of maternal purebreds, but these two hybrid combinations were expected to produce potentially large quantities of hatched larvae. In the above evaluation, promising hybrid combinations were identified for commercial production of seed. For these hybrids to contribute to the development of Korea's mariculture industry, mass production of fertilized eggs and seeds is necessary, along with the development of advanced rearing techniques, such as the identification of a suitable rearing temperature.

• Animal cells and systems
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• v.12 no.4
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• pp.287-295
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• 2008

Variations of larval abundance and hatch timing of Dungeness crabs, Cancer magister Dana 1852, were investigated. Dungeness crab larvae were monthly collected at 16 stations arrayed in four transects, Upper Chatham, Icy Strait, Cross Sound, and Icy Point, in southeastern Alaska from May to September 1997-2004. Larval abundance at all transects was the highest in June except in the Icy Point transect. Larval abundance was the highest in the Icy Strait transect, moderate in the Upper Chatham and Cross Sound transects, and the lowest in the Icy Point transect. Zoeae I(ZI) was predominated in May; thereafter ZI decreased and late zoeal stages occurred. In May and June, small numbers of late stage larvae unusually co-occurred with ZI in three transects. These late stage larvae may have been transported from where hatching occurs earlier. The timing of ZI occurrence varied interannually and was related to degreedays during the egg incubation period of Dungeness crabs: later larval hatching in 1997 and 2002 when temperatures were colder, while earlier larval hatching in 1998 when temperatures were warmer. The distribution patterns of Dungeness crab larvae in southeastern Alaska were markedly different from those reported from other areas of the species distribution ranges: larvae occurring much later in the year, and late stage larvae occurring in inland waters.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.1
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• pp.23-29
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• 2022

This study was carried out to clarify the egg, larvae and juveniles development of Misgurnus anguillicaudatus, and relationships of M. anguillicaudatus and M. mizolepis, Cobitididae Fishes. The adult fishes were collected in Samsan-cheon, Haenam-gun, Jeollanam-do, Korea and their spawning inducement was carried by ovaprim injections. The egg shape was circular and the size was average 1.12 mm. The eggs were hatched at 61 to 72 h after fertilization. The newly hatched larvae had an average 3.23 mm in total length (TL). At 5 days after hatching, the larvae reached to post larval stage and they were 10.3 mm in TL. At 19 days after hatching, it reached to juvenile stage and was 25.3 mm in TL. The egg size of M. anguillicaudatus was almost same as M. mizolepis but the hatching period of M. anguillicaudatus has taken longer. It was possible for interspecific distinguishability of M. anguillicaudatus and M. mizolepis when their larvae reached to juvenile stage by the development of keel-like ridges.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.5
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• pp.676-683
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• 2023

The development of the egg, larvae, and juveniles of Acheilognathus macropterus from the Wicheon stream of Nakdonggang River was investigated for early life history characteristics. The fish used in the study were collected from Wicheon stream located in Bian-myeon, Uiseong-gun, Gyeongsangbuk-do. The fertilized egg was 1.88±0.09 mm (n=30) in long diameter and 1.34±0.06 mm (n=30) in short diameter. After fertilization at a water temperature of 20℃, the egg took 31 h and 40 min to hatch. Newly hatched larvae had an average total length of 3.92±0.13 mm (n=30) and possessed a yolk sac the mouth and anus were not developed. Twenty days after hatching, the postflexion larvae had an average total length of 8.08±0.29 mm (n=30), with the tail tip fully bent at 45° and began feeding. Thirty-three days after hatching, the number of fin stems reached an integer with an average total length of 15.0±1.08 mm (n=30). In this study, differences in the egg size, hatching time, melanophore development period, and distribution location were confirmed between A. macropterus and allied species.

• Journal of Aquaculture
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• v.20 no.4
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• pp.239-247
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• 2007

We studied spawning, hatching and larval growth of Amphiprion melanopus under laboratory conditions. Each time a fish spawned about 440 to 650 eggs. The eggs were hatched 9 days later. Nest colour of the eggs changed from clear yellow orange to greyish violet with processing of embryogenesis. With regard to spawning according to the substrates, the fish prefered red clay pot and ceramic pot to volcanic scoria and live rock. Hatching rate (%) of the eggs on the former substrates was significantly higher than that on the latter. The number of eggs varied with the food stuff supplied to the fish. The fish fed Tetra bits (commercial diet), meat of short necked clams and mussels, and astaxanthin showed significantly higher fecundity with 638 eggs than that fed only Tetra bits (477 eggs) or Tetra bits with meat of shellfish without astaxanthin ($549{\sim}553\;eggs$). Higher temperature ranging from 24 to $30^{\circ}C$ induced shorter incubation period and smaller total length, but higher hatching rate. The optimum salinity and L:D cycle with $60\;{\mu}mol$ photon $m^{-2}s^{-1}$ for hatching were 33 psu and 16:8, respectively. The newly hatched larvae about 4.6 mm in total length grew to 7.5 mm in one week, 18.8 mm in a month and 37.9 mm in 70 days after hatching.

• Korean Journal of Veterinary Research
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• v.33 no.2
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• pp.277-283
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• 1993

In the present study, observations were made on the life-history of Lymnaea viridis under laboratory conditions, involving incubation period of the eggs and their hatching rate, shell length of the newly hatched snails, sexual maturity, size of the snails when the snail produced the first egg-mass, the number of eggs in each egg-mass, egg-laying, ovipostion, growth rate of the snails, and longevity of the snail. At temperatures between $19.8^{\circ}C$ to $22.5^{\circ}C$, incubation period of the eggs occupied 10~12 days, and after beginning of hatching, all young snails emerged completely from the egg-mass within 5 days. The hatching rate was 88%. The average shell length of the newly hatched snails was about 0.064cm. The rate of growth was extraordinarily rapid under good laboratory conditions. When two snails were reared in one culture vessel($20{\times}15{\times}5cm$) with blue-green algae at about $22^{\circ}C$, snail growth was optimal, taking 37 days to reach 1.2cm in shell length. Sexual maturity reached in about 19 days. The size of the snails at sexual maturity was $0.78{\pm}0.05cm$ in length and $0.47{\pm}0.04cm$ in width. The first egg-masses produced were $0.59{\pm}0.22cm$ in length and $0.34{\pm}0.08cm$ in width, and contained 7~38 eggs. The eggs are usually laid in water. The egg-laying was affected by food and temperature. Snails fed with blue-green algae at about $22^{\circ}C$ produced larger egg-masses than the snails fed with fish food at about $26^{\circ}C$. Under conditions of continuous activity and growth, the maximum expectation of life appears to be 109~350(mean 230) days. And the shell length of snails at death were 1.39~1.64cm.

• Korean Journal of Ichthyology
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• v.35 no.3
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• pp.151-159
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• 2023

This study investigated the early life history of the Micropercops swinhonis in Botongcheon stream Hangang River and compared the relationship between the related species and flexibility. The fertilized egg of the ovoid type, and the average egg diameter was 1.23 mm in long diameter and 0.85 mm in short diameter. At a water temperature of 20.6℃, the hatching time took 179 hours. The newly hatched larvae measured 3.82~4.08 mm in total length (TL), and their mouth and anus were not yet open. At 18 days after hatching, the postflexion larvae reached 7.97~8.43 mm in TL, with the tip of the caudal fin bent at 45°. At 29 days after hatching, the juveniles reached 9.83~10.8 mm in TL and were differentiated by their constant number of fins (7~10 first dorsal, 10~11 second dorsal, 7~10 anal fins). As a result of the study, there was a difference from the related species in the egg diameter, the newly hatched larva size. The postflexion larvae period and the development period of the first dorsal fin were the slowest among the comparable simila species, and the size was characterized by a large number, indicating a difference from similar species in the early life history process.

• The Korean Journal of Malacology
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• v.17 no.2
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• pp.85-94
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• 2001

Spawning, hatching, survival and cannibalism of the octopus, Octopus ocellatus, were investigated at the indoor laboratory. The specimens were collected in the coastal waters of Puan, Korea, from March 2000 to May 2001. Changes in the gonadosomatic index (GSI) during March to May, 2000 were similar to those in 2001. The GSI began to increase in March and reached the maximum in May 2000 and 2001. The mean fecundity of adult individuals in April was larger than those in March and May in 2000 and 2001. In the investigations of survival rates of adults according to the water layers, the mean survival rate in the middle water layer was higher than that in the bottom layer in year 2000 (p = 0.002). The survival rate during 2001 was similar to the result of year 2000 in the water layers (p = 0.018). From the investigations with the purchase dates, the survival (88.8%) in the early spawning period (April 10, 2000) showed larger than those in the mid-spawning (May 11, 2000) and the late spawning (May 23, 2000). For aquaculture, it is suggested that the purchase timing of adults in the early spawning period will have a better result than that of the other spawning periods. In the amount of spawning with water layers, the number of eggs spawned of the adults in the middle layer were larger than those in the bottom layer. In the number of eggs spawned with the purchase dates, the number of eggs spawned in the early spawning period were larger than those in the middle and late spawning periods. The size of eggs spawned were 6.904$\times$2.520 mm. the hatching period required (from spawning to hatched larvae) was 55 days (April 17 to June 7,2000) at water temperatures of 11.2 to 21.1$^{\circ}C$. In case of the hatching rates with water layers, the hatching rate in the middle water layer was larger than that in the bottom one (p = 0.004).