• 한국식품과학회지
• /
• 제41권1호
• /
• pp.37-41
• /
• 2009

포도가공 시 발생되는 부산물인 포도씨로부터 유용성분인 vitamin E 및 tocotrienol을 초임계 유체추출방법으로 최적화 하였다. 추출 온도(30-$50^{\circ}C$, $X_1$), 추출압력(17-25MPa, $X_2$) 및 추출시간(1-5 hr, $X_3$)을 변수로 반응표면 분석법으로 vitamin E 및 tocotrienol 함량을 측정하였다. 초임계 이산화탄소상에서 vitamin E 및 tocotrienol 함량은 추출온도, 압력 및 시간이 증가함에 따라 증가하였다 (plt;0.001). 각각의 추출조건 가운데 $40^{\circ}C$, 20 MPa 및 5 hr에서 vitamin E는 8.65 mg/100 g 그리고 tocotrienol은 7.88 mg/100 g으로 가장 높은 함량을 나타내었다. 반응표면분석에 의한 최적추출조건은 $40^{\circ}C$, 20.7MPa 및 3.8 hr으로 나타났으며, 이 때의 vitamin E 및 tocotrienol 함량은 각각 8.20 mg/100 g 및 7.42 mg/100 g으로 나타났다. Vitamin E 및 tocotrienol 함량에 대한 실측값은 통계적 예측값의 각각 99.27-106.10% 및 96.09-106.60%로 나타나 추출조건의 유효성이 인정되었다. 또한 유기용매 추출시 vitamin E 및 tocotrienol 함량은 각각 8.18 mg/100 g 및 7.24 mg/100 g이었다. 따라서 용매추출법과 초임계 이산화탄소 추출법을 비교했을 때 초임계 이산화탄소 추출법을 이용하는 것이 더 효과적으로 vitamin E 및 tocotrienol을 추출할 수 있을 것으로 판단되었다.

• 천문학회보
• /
• 제37권2호
• /
• pp.95-95
• /
• 2012

Constellations are formed of bright stars which appear close to each other on the sky, but are really far apart in space. The shapes you see all depend on your point of view. Back before people had televisions and electricity to light their homes at night, they spent a lot more time looking at the stars. People all over the world used their imaginations to draw pictures in the sky, as if it were a giant connect-the-dot game. The patterns they imagined are called constellations. People usually saw patterns that reflected their different cultures. Native Americans in North America imagined many animals and shapes from the natural world. The ancient Greeks found images of gods and goddesses in the stars. Sometimes people from very different parts of the world even imagined the same animal or shape in the same stars. Most of the constellations we recognize today were made up by the ancient Greeks around 6,000 years ago. Different constellations are visible at different times of year, so the first appearance of these patterns told farmers of the changing seasons and reminded them to plant or harvest their crops. The constellations also help us to find our way around the night sky and to remember which stars are which. The star names we use today are mostly from Greek and Arabic, but many are changed a bit from the original, as often happens when words are passed from one language to another. It can be difficult to picture just what those folks long ago were seeing in the stars, so don't be discouraged if you have trouble seeing their patterns. You can even make up your own! In ancient world all the countries or regions had their own way to name things up in the sky, make up stories and draw different shapes for constellations. Today there are 88 official constellations, but you may find that different books show their stars connected in slightly different ways. The official constellations are specific regions of the sky, so the exact patterns are not all that important. However in various cultures there are some famous star patterns that use stars from only apart of a constellation, or even connect stars from different constellations. These patterns of stars that are not official constellations are called asterisms. The Big Dipper is a very famous asterism, found in the constellation Ursa Major, or Great Bear.

• 한국조경학회지
• /
• 제22권4호
• /
• pp.133-148
• /
• 1995

This study was carried out to provide behavioral data for its planning and management of civic garden through the evaluation of user's motivation & satisfaction. The civic gardens around Seoul were surveyed. The site are located in Kwangtan-myeon, Sudong-myeon, Wonsam-myeon, Nam-myeon, Buknae-myeon, and Jumdong-myeon, A total of 244 questionnaires were completed by mail questionnaires. The results are as follows: 1. 82 percentage of users are in thirties or fourties most of them have children who go to the elementary school. 50 percentage of users are just typical house wifes. 74 percentage of users graduated form university. 60 percentage of users live in lofty apartment buildings. 2. As a result from factor analysis, their motivations are categorized into four fator groups.: 'to experience nature', 'weekend recreation', 'nostalgia', 'to provide their old parent's sparetime'. And their satisfactions are categorized into eleven fator groups.: 'psychological/intellectual component', 'recreational components', 'instruction/management', 'facility'. 'vegetable cultivation', 'social contact', 'crowing', 'aesthetic component', 'family contact', 'the terms of lease', 'visition time'. 3. The user of civic garden can be divided into four user groups by their motivation. CLUSTER1 can represent the user group who have motivations for 'leisure, relaxation'. They are considerably satisfied with all other factor 'opportunity of meeting new person'. CLUSTER2 at the age of 41 to 50 have motivation for 'health, nostalgia'. CLUSTER3 at the age of 31 to 40 have motivation for 'harvest, experiencing nature'. CLUSTER4 at the age of more than 51 want to let their parents enjoy their sparetime. They are dissatisfied with accessibility, amount of cultivation area, crowding and overall farm management. 4. The regression analysis was employed with predicting the overall satisfaction. The results of regression analysis showed that 69% of total variances was explained by six variables: The most effective variable is 'whether visiting on weekend or weekdays', the visitors on weekdays are far more satisfied than weekend visitors because of traffic congestion, and crowding. The second source of satisfactions are 'psychological/intellectual components', they are satisfied with 'family contact', 'the terms of lease' and 'instruction in farming' are sources of satisfaction or dissatisfaction, and finally 'aesthetic landscape' is the source of satisfaction. The second most important variable is psychological one. Even though the civic gardens were not well equipped, they liked the atmosphere of rural life, refreshness, nostalgia, satisfaction from cultivation plants, and sense of achievement.

• 정보처리학회논문지:소프트웨어 및 데이터공학
• /
• 제7권4호
• /
• pp.129-134
• /
• 2018

최근, 인공신경망 모델은 예측, 수치제어, 로봇제어, 패턴인식 등의 분야에서 촉망되는 기술이다. 본 연구에서는 인공신경망 모델을 이용하여 온실 외부 온도를 예측하고 이를 온실제어에 활용하는데 목적이 있다. 예측 모델의 성능 평가를 위해 다중회귀모델과 SVM 모델과의 비교분석을 수행하였다. 평가 방법으로는 10-Fold Cross Validation을 사용하였으며, 예측 성능 향상을 위해 상관관계분석 통해 데이터 축소를 수행하였고, 측정 데이터로부터 새로운 Factor 추출하여 데이터의 신뢰성을 확보하였다. 인공신경망 구축을 위해 Backpropagation algorithm을 사용하였으며, 다중회귀모델은 M5 method로 구축하였고, SVM 모델을 epsilon-SVM으로 구축하였다. 각 모델의 비교분석 결과 각각 0.9256, 1.8503과 7.5521로 나타났다. 또한 예측모델을 온실 난방부하 계산에 적용함으로써 온실에 사용되는 에너지 비용 절감을 통한 수입증대에 기여할 수 있다. 실험한 온실의 난방부하는 3326.4kcal/h이며, 총 난방시간이 $10000^{\circ}C/h$일 때 연료소비량은 453.8L로 예측된다. 아울러 데이터 마이닝 기술 중 하나인 인공신경망을 정밀온실제어, 재배기법, 수확예측 등 다양한 농업 분야에 적용함으로써 스마트 농업으로의 발전에 기여할 수 있다.

• 한국작물학회지
• /
• 제62권4호
• /
• pp.367-372
• /
• 2017

전북지역에서 고품질 가공용 고구마 생산을 위한 적품종 선발을 위하여, 국내 육성된 14품종을 재료로 30 cm 크기의 종순을 $75{\times}25cm$로 5월 30일 정식하여 배색비닐로 멀칭재배한 다음, 120일 및 150일 수확에 따른 생육 및 수량 특성 등을 조사하였다. 정식 30일째의 줄기신장은 '다호미'와 '신황미'에서 90 cm 이상으로 왕성하였고, 정식 120일째에는 '신황미', '신율미', '대유미', '진홍미', '연황미' 등에서 250 cm 이상으로 줄기신장이 왕성하였다. 주당 분지수는 정식 30일째에는 '신자미'에서, 120일째에는 '신황미'에서 가장 많았다. 주당상저중, 평균상저중, 주당상저수 및 상저수량은 정식 120일 후 수확보다 150일 후 수확에서 유의한 증가를 보였다. 정식 120일 후 수확에서 상저수량은 '전미', '다호미', '대유미'에서 2.5톤/10a 이상으로 높았으며, 150일 후 수확에서 세 품종의 상저수량은 3.2-3.5톤/10a로 크게 증가하였다. 정식 30일째의 줄기길이 및 마디수는 주당상저중 및 상저수량과 유의한 정의 상관을 보였다. 품종 고유특성인 전분가는 정식 120일 후 수확보다 150일 후 수확에서 유의한 증가를 보였다. 따라서 전북지역 가공용 품종으로는 전분가가 높고 다수성으로 생육이 양호한 '전미', '대유미'와 점질품종으로 담주황색 육질을 가진 '다호미' 등이 유망하였다.

• Journal of Microbiology and Biotechnology
• /
• 제16권10호
• /
• pp.1583-1590
• /
• 2006

T4 endonuclease V (T4 endo V) [EC 3. 1. 25. 1], found in bacteriophage T4, is responsible for excision repair of damaged DNA. The enzyme possesses two activities: a cyclobutane pyrimidine dimer DNA glycosylase (CPD glycosylase) and an apyrimidic/apurinic endonuclease (AP lyase). T4 denV (414 bp cDNA) encoding T4 en do V (138 amino acid) was synthesized and expressed using either an expression vector, pTriEx-4, in E. coli or a baculovirus AcNPV vector, pBacPAK8, in insect cells. The recombinant His-Tag/T4 endo V (rHis-Tag/T4 endo V) protein expressed from bacteria was purified using one-step affinity chromatography with a HiTrap Chelating HP column and used to make rabbit anti-His-Tag/T4 endo V polyclonal antibody for detection of recombinant T4 endo V (rT4 endo V) expressed in insect cells. In the meantime, the recombinant baculovirus was obtained by cotransfection of BacPAK6 viral DNA and pBP/T4 endo V in Spodoptera frugiperda (Sf21) insect cells, and used to infect Sf21 cells to overexpress T4 endo V protein. The level of rT4 endo V protein expressed in Sf21 cells was optimized by varying the virus titers and time course of infection. The optimal expression condition was set as follows; infection of the cells at a MOI of 10 and harvest at 96 h post-infection. Under these conditions, we estimated the amount of rT4 endo V produced in the baculovirus expression vector system to be 125 mg/l. The rT4 endo V was purified to homogeneity by a rapid procedure, consisting of ion-exchange, affinity, and reversed phase chromatographies, based on FPLC. The rT4 endo V positively reacted to an antiserum made against rHis-Tag/T4 endo V and showed a residual nicking activity against CPD-containing DNA caused by UV. This is the first report to have T4 endo V expressed in an insect system to exclude the toxic effect of a bacterial expression system, retaining enzymatic activity.

• Journal of Biosystems Engineering
• /
• 제25권3호
• /
• pp.213-220
• /
• 2000

Grafting of fruit-bearing vegetables has been widely used to increase the resistance to soil-borne diseases, to increase the tolerance to low temperature or to soil salinity, to increase the plant vigor, and to extend the duration of economic harvest time. After grafting, it is important to control the environment around grafted seedlings for the robust joining of a scion and rootstock. Usually the shading materials and plastic films are used to keep the high relative humidity and low light intensity in greenhouse or tunnel. It is quite difficult to optimally control the environment for healing and acclimation of grafted seedlings under natural light. So the farmers or growers rely on their experience for the production of grafted seedling with high quality. If artificial light is used as a lighting source for graft-taking of grafted seedlings, the light intensity and photoperiod can be easily controlled. The purpose of this study was to develop a prototype system for the graft-taking enhancement of grafted seedlings using artificial lighting and to investigate the effect of air current speed on the distribution of air temperature and relative humidity in a graft-taking enhancement system. A prototype graft-taking system was consisted by polyurethane panels, air-conditioning unit, system controller and lighting unit. Three band fluorescent lamps (FL20SEX-D/18, Kumho Electric, Inc.) were used as a lighting source. Anemometer (Climomaster 6521, KANOMAX), T-type thermocouples and humidity sensors (CHS-UPS, TDK) were used to measure the air current speed, air temperature and relative humidity in a graft-taking system. In this system, air flow acted as a driving force for the diffusion of heat and water vapor. Air current speed, air temperature and relative humidity controlled by a programmable logic controller (UP750, Yokogawa Electric Co) and an inverter (MOSCON-G3, SAMSUNG) had an even distribution. Distribution of air temperature and relative humidity in a graft-taking enhancement system was fairly affected by air current speed. Air current speed higher than 0.1m/s was required to obtain the even distribution of environmental factors in this system. At low air current speed of 0.1m/s, the evapotranspiration rate of grafted seedlings would be suppressed and thus graft-taking would be enhanced. This system could be used to investigate the effects of air temperature, relative humidity, air current speed and light intensity on the evaportranspiration rate of grafted seedlings.

• 방사성폐기물학회지
• /
• 제8권2호
• /
• pp.151-158
• /
• 2010

무에 대한 $I_2$ 증기의 작물체 침적속도와 뿌리 전류계수를 측정하기 위하여 파종 후 29 일에서 53 일 사이에 생육시기별로 작물체를 $I_2$ 증기에 80 분 간 피폭시켰다. 피폭은 오전 중에 투명한 상자 내에서 수행되었다. 침적속도($ms^{-1}$)는 대체로 $1.0{\times}10^{-4}{\sim}2.0{\times}10^{-4}$의 범위로 생육밀도가 높을수록 증가하는 경향이었다. 또한 상대습도가 높을 경우 값이 커진다는 기존 보고와 어느 정도 일치하였다. 본 침적속도는 몇몇 야외 측정치보다 수 십 배 정도 낮았고 이는 주로 피폭상자 내의 낮은 풍속($0.2\;ms^{-1}$ 내외)에 기인하는 것으로 추정되었다. 뿌리 전류계수(작물체 총침적량에 대한 수확시 뿌리 내 함유량의 비)는 다소 보수적으로 계산하여 파종 후 29 일 피폭에서 $1.3{\times}10^{-3}$, 파종 후 53 일 피폭에서는 $5,0{\times}10^{-3}$이었다. 본 실험결과의 이용에 있어서는 기상 조건, 요오드의 물리화학적 형태 등에 유의할 필요가 있다.

• 식물병연구
• /
• 제14권1호
• /
• pp.57-60
• /
• 2008

2006년부터 2007년까지 2년 동안 진주시 농산물도매시장에 유통, 판매중인 사과(품종: 후지)에서 이상증상이 발생하였다. 병징은 상처나 열과된 과실이 수침상으로 물러지면서 빠르게 부패하였다. 균총은 처음 흰색에서 연한 갈색으로 되며 검은색의 포자낭을 많이 형성하며 균사생육 적온은 $25^{\circ}C$였다. 포자낭경은 처음 흰색에서 암갈색을 나타내며, 크기는 $950{\sim}1,900\;{\mu}m$이고 폭은 $12{\sim}22\;{\mu}m$이었다. 포자낭은 처음에 흰색에서 검은색으로 되며 모양은 구형 또는 반구형으로 크기는 $82{\sim}185\;{\mu}m$이었다. 주축의 모양은 반구형으로 크기는 $70{\sim}85\;{\mu}m$이었다. 포자낭포자는 담갈색으로 단포이며 구형 또는 타원형으로 크기는 $8{\sim}18{\times}7{\sim}8\;{\mu}m$이었다. 이상과 같이 병원균의 균학적 특징과 병원성을 검정한 결과, 본 병해를 Rhizopus stolonifer(Ehrenb.) Vuill.에 의한 사과 무름병으로 명명할 것을 제안한다.

• Archives of Plastic Surgery
• /
• 제34권4호
• /
• pp.432-435
• /
• 2007

Purpose: The jejunal free flap has the shorter ischemic time than other flap and requires a laparotomy to harvest it. As the evaluation of the perfusion the buried flap is very important, the perfusion of the buried jejunal free flap requires monitoring for its salvage. We tried to improve the monitoring flap method in the jejunal free flap and examined its usefulness. Methods: From March 2002 to March 2006, the monitoring flap method was applied to 4 cases in 8 jejunal free flaps for the pharyngeal and cervical esophageal reconstructions. The distal part of the jejunal flap was exposed without suture fixation through cervical wound for monitoring its perfusion. The status of perfusion was judged by the color change of jejunal mucosa and mesentery. If necessary, pin prick test was performed. Doppler sonography was applied to mesenteric pedicle of the monitoring flap in case of suspicious abnormal circulation. Results: The monitoring flap shows no change in 3 cases, but the congestion happened in one case at the 12 hours after the operation. This congestion was caused by the twisting or kinking of the mesenteric pedicle of the monitoring flap. So, we fixed up the monitoring flap close to adjacent cervical skin for prevention of rotation. Finally, the main part of transferred jejunal flap was intact. Conclusion: The success of a jejunal free flap depends on close postoperative monitoring and early detection of vascular compromise. So, various monitoring methods have been tried, for instance, direct visualization using a fiberoptic pharyngoscope, through a Silastic window placed in the neck flap, or external surface monitoring with an Doppler sonography, use of a buried monitoring probe. But, all of the above have their own shortcomings of simplicity, non-invasiveness, reliability and etc. In our experience, monitoring flap can be a accurate and reliable method.