• Title/Summary/Keyword: Half Life

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Biochemical Properties of Recombinant Cyclodextrin Glucanotransferase Expressed in Saccharomyces cerevisiae (Saccharomyces cerevisiae에 발현된 재조합 cyclodextrin glucanotransferase의 생화학적 특성)

  • 박현이;남수완;김병우
    • Journal of Life Science
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    • v.11 no.3
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    • pp.230-234
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    • 2001
  • The cyclodextrin glucanotransferase(CGTase) gene of Bacillus macerans was expressed in Saccharomyces cerevisiae and the recombinant CGTase was partially purified from the yeast culture supernatant. The optimal pH and temperature of the CGTase were found to be 6.0 and 5$0^{\circ}C$, respectively. The pH and temperature stabilities of the recombinant enzyme were significantly enhanced and the half life at 55$^{\circ}C$ was about 60 hr. When the recombinant CGTase was reacted with 5% soluble starch, the conversion yield of total cyclodextrin (CD) from starch was estimated to be 41% at 48 hr, whereas the wild type enzyme showed the yield of 12%. This improvement of conversion yield and thermal stability of CGTase may be useful for the development of low-cost CD production process.

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Distributions of Local Supply and Exhaust Effectiveness according to Room Airflow Patterns

  • Han, Hwa-Taik;Choi, Sun-Ho;Lee, Woo-Won
    • International Journal of Air-Conditioning and Refrigeration
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    • v.10 no.4
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    • pp.177-183
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    • 2002
  • A pulsed tracer gas technique is applied to measure the distributions of local mean age and local mean residual-life-time of air in a half-scale experimental chamber, The airflow patterns in the chamber are visualized by a Helium bubble generator for three different exhaust locations. A supply slot is located at the top of a right wall, and an exhaust slot is at either bottom-left (Case 1), bottom-right (Case 2), or top-left (Case 3) location. Results show that the distributions of local mean age and local mean residual-life-time are different from each other, but both of them are closely related to the airflow pattern in the space. Included are discussions on explaining the variations of overall room ventilation effectiveness depending upon airflow rates for three different supply-exhaust configurations.

Wall Thickness Measurement of Respiratory Airway in CT Images: Signal Processing Aspects

  • Park, Sang-Joon;Kim, Jong-Hyo;Kim, Kwang-Gi;Lee, Sang-Ho
    • Proceedings of the IEEK Conference
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    • 2007.07a
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    • pp.279-280
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    • 2007
  • Airway wall thickness is an important bio-marker for evaluation of pulmonary diseases such as stenosis, bronchiectasis. Nevertheless, an image-based analysis of the airway tree can provide precise and valuable airway size information, quantitative measurement of airway wall thickness in CT images involves various sources of error and uncertainty. So we have developed an accurate airway wall measurement technique for small airways with three-dimensional (3-D) approach. To illustrate performance of these techniques, we used airway phantom that consisted of 4 acryl tubes with various inner and outer diameters. Results show that evaluation of interpolation and deconvolution methods of airways in 3-D CT images, and significant improvement over the full-width-half-maximum method for measurement of not only location of the luminal and outer edge of the airway wall but airway wall thickness.

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Fault-tree based reliability analysis for paralleled half-bridge sub-module of HVDC (HVDC 병렬 하프브리지 서브모듈에 대한 고장나무기반의 신뢰성 분석)

  • Kang, Feel-soon;Song, Sung-Geun
    • Journal of IKEEE
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    • v.23 no.4
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    • pp.1218-1223
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    • 2019
  • In HVDC systems, the full-bridge submodule increases the number of components compared to the half-bridge submodule, but the failure-rate can be reduced by securing 100 % redundancy. However, full-bridge submodules require more complex control algorithms to ensure the redundancy and to prevent arm-short with sufficient dead-time. To solve this problem, we analyse the failure-rate of the paralleled half-bridge configuration with the same number of components and 100 % redundancy as the full-bridge submodule. The fault tree analysis (FTA) method is applied to the conventional part failure analysis to reflect the operation risk of the submodule, thereby predicting the life-cycle of the submodule more accurately. To verify the validity, the failure-rate results of the proposed FTA based analysis method are compared with the failure rate obtained by the part failure method.

Impact on Interpersonal Relationship Skills of Children: Social Demographic Variables, Marital Perspective-taking Ability, Children Managing Responses and Emotional Intelligence (유아의 대인관계형성능력에 영향을 미치는 변인 연구: 사회 인구학적 변인, 부부조망수용능력, 유아 대처반응 및 정서지능을 중심으로)

  • Jang, Yun-Hee;Moon, Hyuk-Jun
    • Journal of Families and Better Life
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    • v.30 no.3
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    • pp.179-192
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    • 2012
  • The purpose of this study is to investigate the variable factors influencing children's interpersonal relationship skills and analyze the effects of marital perspective-taking ability, managing responses of young children and emotional intelligence on the interpersonal relationship skills of children. The study subjects were young children aged from four to seven attending private kindergartens or public day care centers in Seoul or Kyeongki-do and their mothers. The results are as follows : 1) First, marital perspective-taking abilities were indicated higher on boys than girls, children in a full-day program than those in a half-day program, university graduates or higher degree holders than collage graduates, single-income families than double-income families. In case of managing responses of young children, boys showed high avoidance of offensive disposition and girls showed high emotions dissipation. Also, young children in a full-day program showed non-responses on managing responses but high responses on emotions dissipation, and children of mothers with bachelor's degree or higher education showed support and children from double-income family showed no responses in high. Moreover, emotional intelligence of young children in a full-day program was higher than that of children in a half-day program, interpersonal relationship skills were showed higher on girls than boys, half-day children than full-day ones, children from single-income family than those from double-income family. 2) Meaningful relationships were indicated between these variables; managing responses of young children, emotional intelligence and interpersonal relationship skills by partly showing correlations. 3) Children in a half-day program with low in emotions dissipation, non-responses, offensive dispositional managing responses but high on Avoid, emotional intelligence showed high interpersonal relationship skills.

Effect of a Metal-strap Thicknesses on the Bending Process

  • Jung, In-Suk;Kim, Jung-Whan;Lee, Weon-Hee;Chang, Jun-Pok;Bae, Hyun-Mi
    • Journal of the Korean Wood Science and Technology
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    • v.29 no.3
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    • pp.14-20
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    • 2001
  • On the bending process, metal-strap plays an important role in dispersing the stress generated in wood. Therefore, the metal-strap has more influence on the property of bentwood materials. The effect of the metal-strap thickness for bentwood was examined. The effect of metal-strap on the bending properties of Korean red pine(Pinus densiflora Sieb. et Zucc.)was investigated in this research. The metal-strap thickness is divided into 4 kinds such as 1.0, 0.8, 0.6, 0.4 mm. The specimens were selected by grain such as annual ring angles, flat grain and half-edge grain specimens. As a result of this study, the bending ability of 1.0, 0.8 mm, thickness of half-edge grain specimens was better than flat grain specimens but the result of 0.6, 0.4 mm were reversed. The bending ability of half-edge grain was better than flat grain and the grade was higher. When the processed specimens were dried, the radius of curvature(ROC) was decreased became drying-stress was not perfectly dispersed. An optimum drying-condition would deminish this phenomenon.

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Analysis of Joint Movements and Changes of Muscle Length During STS(sit-to-stand) at Various Sitting Heights in the Korean Elderly's daily life (한국 고령자의 일상생활 중 다양한 높이에서의 STS(sit-to-stand) 시 관절운동 특성 및 근길이 변화 분석)

  • Hwang, S.J.;Son, J.S.;Kim, J.Y.;Kim, H.D.;Lim, D.H.;Kim, Y.H.
    • Journal of Biomedical Engineering Research
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    • v.29 no.6
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    • pp.484-492
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    • 2008
  • Sit to stand(STS) movement is one of the most common activity in daily life. In addition, Korean traditionally stand up from various sitting heights in one's daily life compared to other foreigners. As Korea enter rapidly to the aging society, needs of the elderly's independent life are increasing. Therefore the importance of research about the analysis of elderly's activity in daily life is rapidly increasing. In this study, we analyzed joint movements and changes of muscle length during STS(sit-to-stand) at various sitting heights(table seat, bath seat, bottom) in the Korean elderly's daily life by using the motion analysis and musculoskeletal modeling. Ten Korean elderly and young were participated in this experiment. Three heights of sitting posture which could represent typical sitting in Korean daily life were chosen as table seat(42cm), bath seat(21cm) and bottom(0cm). As the results, the elderly showed both smaller knee/hip flexion and larger trunk flexion relatively in comparison to the young during table seat STS. The elderly also showed larger dorsiflexion and smaller ROM of knee, hip, trunk compared to the young during bath seat STS. Additionally, the elderly showed larger plantarflexion, hip flexion, smaller knee flexion and trunk flexion during the first half of bottom STS and larger knee flexion, hip flexion and trunk flexion during the second half of bottom STS. In addition, we could know contraction and relaxation characters of major muscles in lower limb during various STS through the analysis of changes in muscle length by musculoskeltal modeling.

Establishment of Pre-Harvest Residue Limits (PHRLs) of Fluopicolide and Metrafenone in Cherry Tomato (Lycopersicon esculentum Mill.) (방울토마토(Lycopersicon esculentum Mill.) 생산단계에서 Fluopicolide 및 Metrafenone의 잔류허용기준 설정)

  • Hur, Kyung Jin;Woo, Min Ji;Kim, Ji Yoon;Saravanan, Manoharan;Kwon, Chan-Hyeok;Son, Yong Wook;Hur, Jang Hyun
    • Korean Journal of Environmental Agriculture
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    • v.34 no.4
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    • pp.328-335
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    • 2015
  • BACKGROUND: The present investigation was aimed to predict the pre-harvest residue limits (PHRLs) of the fluopicolide and metrafenone on cherry tomato and to estimate their half-life and characteristics of the residues.METHODS AND RESULTS: Pesticides were treated once on cherry tomato in field 1 and 2 under the standard application rate. The samples were collected 7 times at the end of 0(2 hours after pesticides spaying), 1, 2, 3, 5, 7 and 10 days before harvest. Residues of fluopicolide and metrafenone were analyzed by the LC-MS/MS. In this study, the method limit of quantification (MLOQ) for both fluopicolide and metrafenone in cherry tomato was found to be 0.005 mg kg-1. Their recovery levels were 92.7∼94.8% and 82.6∼88.0%, shown with coefficient of variation of less than 10%. Half-life of fluopicolide and metrafenone in field 1 and 2 were found to be 15.0 days and 12.8 days, 18.9 days and 21.5 days, respectively.CONCLUSION: Based on the results, this study shows the level of PHRL on cherry tomato is 0.27 mg/kg for fluopicolide and 2.29 mg/kg for metrafenone at 10 days before harvesting. The present study indicates the residues of both pesticides on cherry tomato will be below maximum residue limit (MRL) at harvest.

Residue Patterns of Procymidone, Chlorpyrifos and Cypermethrin in Peaches During Cultivation and Storage Period (복숭아의 재배 및 저장기간 중 Procymidone, Chlorpyrifos 및 Cypermethrin의 잔류량 변화)

  • Lee, Yong-Jae;Ko, Kwang-Yong;Won, Dong-Jun;Gil, Geun-Hwan;Lee, Kyu-Seung
    • Korean Journal of Environmental Agriculture
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    • v.22 no.3
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    • pp.220-226
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    • 2003
  • The residue patterns of procymidone, chlorpyrifos and cypermethrin in peaches were examined. The pesticides were sprayed at 15 days before harvest and then were determined the residue at 0, 1, 2, 3, 5, 7, 10 and 15 days after pesticide application and calculated their $DT_{50}$. Also, the degradation patterns at $4^{\circ}C$ and $20^{\circ}C$ during storage period were compared. Biological half-lives of procymidone, chlorpyrifos and cypermethrin in peaches during the cultivation period were 3.1, 7.2 and 10.4 days, respectively. The biological half -life of procymidone was shorter than the others. During the storage period, half-lives of procymidone, chlorpyrifos and cypermethrin were 16.0, 14.3 and 13.1 days at $4^{\circ}C$ and 4.6, 10.2 and 12.9 days at $20^{\circ}C$, respectively. The degradation rates of these three pesticides in storage period were slower than them in cultivation period. Removal rates were $22.2{\sim}82.9%$ by tap water, and $12.5{\sim}88.8%$ by detergent solution.

Evaluation of Half Cell Potential Measurement in Cracked Concrete Exposed to Salt Spraying Test (염해에 노출된 균열부 콘크리트의 반전위 평가)

  • Kim, Ki-Bum;Park, Ki-Tae;Kwon, Seung-Jun
    • Journal of the Korea Concrete Institute
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    • v.25 no.6
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    • pp.621-630
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    • 2013
  • Several techniques for steel corrosion detection are proposed and HCP (half cell potential) technique is widely adopted for field investigation. If concrete has cracks on surface, steel corrosion is rapidly accelerated due to additional intrusion of chloride and carbon dioxide ions. This study is for an evaluation of HCP in cracked concrete exposed chloride attack. For this work, RC (reinforced concrete) beams are prepared considering 3 w/c ratios (0.35, 0.55, and 0.70) and several cover depths (10~60 mm) and various crack widths of 0.0~1.0 mm are induced. For 35 days, SST (salt spraying test) is performed for corrosion acceleration, and HCP and corrosion length of rebar are evaluated. With increasing crack width, w/c ratios, and decreasing cover depth, HCP measurements increase. HCP evaluation technique is proposed considering the effects of w/c ratios, crack width, and cover depth. Furthermore anti-corrosive cover depths are obtained through Life365 program and the results are compared with those from this study. The results shows relatively big difference in cracked concrete, however provide similar anti-corrosive conditions in sound concrete.