• The Plant Pathology Journal
• /
• 제23권3호
• /
• pp.223-225
• /
• 2007

Many plant growth-promoting rhizobacteria (PGPRs) have been known for beneficial effects on plants including biological control of soilborne pathogens, induced systemic resistance to plant pathogens, phytohormone production, and improvement of nutrient and water uptake of plants. We developed a simple and rapid method for screening potential PGPR, especially phytohormone producing rhizobacteria, or for analyzing their functions in plant growth using cucumber seedling cuttings. Surface-sterilized cucumber seeds were grown in a plastic pot containing steamed vermiculite. After 7 days of cultivation, the upper part 2 cm in length of cucumber seedling, was cut and used as cucumber cuttings. The base of cutting stem was then dipped in a microcentrifuge tube containing 1.5ml of a bacterial suspension and incubated at $25^{\circ}C$ with a fluorescent light for 10 days. Number and length of developed adventitious roots from cucumber cuttings were examined. The seedling cuttings showed various responses to the isolates tested. Some isolates resulted in withering at the day of examination or in reduced number of roots developed. Several isolates stimulated initial development of adventitious roots showing more adventitious root hair number than that of untreated cuttings, while some isolate had more adventitious root hair number and longer adventitious roots than that of untreated control. Similar results were obtained from the trial with rose cuttings. Our results suggest that this bioassay method may provide a useful way for differentiating PGPR's functions involved in the development of root system.

• 한국산학기술학회논문지
• /
• 제15권3호
• /
• pp.1311-1317
• /
• 2014

본 연구는 미용업 종사자들의 감정노동, 스트레스 대처행동이 직무긴장에 미치는 영향을 파악하여 미용업 관련 종사자들의 직무긴장 완화와 직무효율성 증진을 위한 기초자료를 제공하고자 시도된 서술적 조사연구이다. 대상자들의 직무긴장에 영향을 미치는 요인을 다중회귀 분석한 결과, 감정노동의 표면행위와 문제중심 대처, 무관심 대처, 사회적 지지대처가 직무긴장에 30% 영향을 미치는 요인으로 나타났다. 대상자들의 감정노동 중 표면행위는 직무긴장과 순상관관계가 있으며, 스트레스 대처행동에서 문제중심대처와 사회적 지지대처와는 역상관관계가 있는 것으로 나타났다. 감정노동 중 진심행위는 문제중심대처, 희망대처, 사회적 지지대처, 긍정대처, 긴장해소대처와 순상관관계가 있는 것으로 나타났다. 본 연구결과를 바탕으로 미용업 종사자들의 직무긴장에 대한 중재에 있어서는 감정노동에 대한 중재와 더불어 건강하고 긍정적인 스트레스대처에 대한 교육과 중재 프로그램이 필요하다.

• 한국임상수의학회지
• /
• 제28권3호
• /
• pp.332-335
• /
• 2011

식욕부진 및 구토를 동반한 6세령 중성 수컷 고양이(domestic short hair)가 진료를 위해 지역 동물병원에 내원하였다. 복부방사선 및 초음파검사상 우측 신장의 종대 및 심한 수신증 소견을 나타내었다. 외과적으로 적출한 신장에 대하여 병리학적 검사를 실시하였다. 신장 단면절개 시, 2개의 유백색 종괴 및 심한 신우 종대가 확인되었다. 종괴의 대부분은 단일형태의 분화도가 높은 세관 구조로 이루어져 있었으며, 입방 내지 원주형 세포들이 한 층으로 배열되어 있고 내강에 유두상으로 돌출되어 있었다. 면역조직화학기법을 통해 종양세포는 cytokeratin (CK) MNF116에 대하여 강한 양성반응을 보였으나, CK 7에는 음성으로 나타내었다. 따라서 임상증상, 병리학적 소견 및 면역조직화학기법을 통하여 본 증례는 수신증이 동반된 유두상 신선종으로 확진되었다.

• Applied Microscopy
• /
• 제26권1호
• /
• pp.79-93
• /
• 1996

The differentiation of nail matrix and fine structure of matrix cells were studied with light and electron microscope using specimens from nails of thumb finger in Korean fetuses 14 to 24 weeks old. Fetal nail matrix consisted of two horizontal layers, thicker ventral and thinner dorsal matrices, originating from invagination of epidermis in proximal nail field. Matrix being generally thicker in its distal region than the apex became gradually thickened with increase of the fetal age. Each matrix consisted of single layer of basal cells and multiple layers of squamous cells which are arranged close to and parallel to the central axis of the nail mairix. The process of keratinization of fetal nail matrix was noted to be occured concurrently in the ventral and dorsal matrices along the central axis of matrix toward distal and dorsal direction. Squamous cells became matured with accumulation of tonofilaments, increase of keratohyalin granules, discharge of membrane coating granules, and narrowing of intercellular spaces, thickening of plasma membrane and finally being transformed into horny cells of nail plate. Horny cells of nail plate filled with fibrous elements in the electron dense amorphous substance. These findings of keratinization process of fetal nail matrix appeared to be similar to those of keratinization in epidermis and inner root sheath of the hair. In the nail matrix, however, corresponding region to the keratogenous zone of growing hair follicle was not observed. Vacuolated squamous cells of nail matrix seen on light microscopy was considered to be artefactual product, but squamous cells with condensed small nuclei rarely found adjacent nail plate was considered to be one of the squamous cells with unknown function. Proximal end of nail plate was observed on dorsal surface of nail field distal to the proximal nail fold at 14 and 16 weeks old human embryos. Proximal prolongation of the proximal end of nail plate was occured with advancing fetal age and afterward 21 weeks nail plate invaded into nail matrix. Melanin granule containing cells and Merkel cells were present only on the basal layer of dorsal nail matirx.

• 한국동물위생학회지
• /
• 제22권2호
• /
• pp.113-119
• /
• 1999

The most common cause of death is ostrich chick fading syndrome(OCFS), which is due to bacterial infection during artificial incubation and hatching. Six farmed ostrich chicks aged 3 and 10 days in Chonbuk province, were submitted to Chonbuk Livestock Development and Research Institute for necropsy, Clinically, birds showed hair loss, ocular exudate, lethargy, diarrhea, and subsequently died 3-5 days after onset of clinical signs. Grossly, umbilicus was enlarged. White-yellowish purulent nodules were scattered on the lung and the membrane of air-sac was thickened and had inflamed exudate on the surface in two chicks that died 3 days after hatching. In 10 days-old chick, intestine was shown rodding segmentally. Yolk sac was still retarded and its surface was partially hemorrahgic. The synovial fluid of the leg was yellowish. Microscopically, multifocal purulent exudates were scattered on the lung. Capillary microthrombi in the glomerulus were prominent and tubular epithelia were necrotic. Necrotic hepatocytes were scattered and intestine were congested. Microbiologically, Pseudomonas sp and/or E coli were isolated from air-sac, lung and/or liver. This case suggests that poor hygiene during artificial incubation, hatching or in the first week after hatching may cause high mortality of the ostrich chicks.

• 한국응용곤충학회지
• /
• 제26권4호
• /
• pp.245-249
• /
• 1987

사과품종별(品種別) 사과나무 반점낙엽병(斑點落葉病)의 발병(發病)과 잎의 모용(毛茸) 발생정도(發生程度)를 조사(調査)하고 사과나무 잎의 부위별(部位別) A. mali의 침입정도(浸入程度)와 모용(毛茸)이 병원균(病原菌)의 침입(浸入)과 감염(感染)에 미치는 영향(影響)을 조사(調査)한 결과(結果)는 다음과 같다. A. mali의 이병정도(罹病程度)에 따른 사과나무 품종(品種)의 분포(分布)는 이병엽율(罹病葉率)이 50.1% 이상(以上)되는 이병성품종(罹病性品種)을 조사(調査)한 82품종(品種) 중(中) 15개품종(個品種)(18.3%) 이었으며 주요(主要) 품종(品種)은 나리탄, 인도(印度), 왕령(王令), 스라크림숀, 스카이스파, 아오리 3호 등(等)이었고 이병엽율(罹病葉率)이 50% 이하(以下)되는 저항성품종(抵抗性品種)이 51개품종(個品種)(62.2%)이었는데 주요품종(主要品種)은 홍옥(紅玉), 무쓰, 모리쓰, 조나골드, 쓰가루, 가라, 골덴, 후지, 스파어리브레이즈 등(等)이었으며, 주요품종(主要品種)들의 엽당병반수(葉當病斑數)도 저항성품종(抵抗性品種)보다 이병성품종(罹病性品種)에서 많았다. 이병성품종군중(罹病性品種群中)에서 이병엽율(罹病葉率)이 6월(月)에 20%, 이상(以上)되어 8월(月)에 70% 이상(以上)되는 품종(品種)이 와인삽, 아오리 3호, 스카이스파, 왕령(王令) 등(等)과 6월(月)에는 5% 이상(以上) 되었다가 8월(月)에 70% 이상(以上)되는 품종(品種)이 라리단, 스타크림숀, 인도(印度) 등(等)으로 구분(區分)되었다. 품종별(品種別) 잎에 모용발생정도(毛茸發生程度)는 이병성(罹病性) 품종군(品種群)에서 보다 저항성품종군(抵抗性品種群)에서 대체(大體)로 많았고 동일(同一) 품종(品種)잎에서 모용무제거부위(毛茸無除去部位)보다 모용제거부위(毛茸除去部位)에서 감염율(感染率)이 높은 것으로 보아 잎의 모용(毛茸)은 품종(品種) 저항성(抵抗性) 요인(要因)으로 추정(推定)되었으며 A. mali의 침입(浸入)과 감염(感染)은 잎의 표면(表面)보다 이면(裏面)에서 용이(容易)하였다.

• Journal of Ginseng Research
• /
• 제13권2호
• /
• pp.189-197
• /
• 1989

A new quaternary ammonium compound, hydrolyzed ginseng-saponin quaternary (HGSQ), from Korean ginseng saponin and 2,3-epoxypropyltrimethylammonium chloride has been developed as a conditioning agent in cosmetics. This structure has a hydrophobic group from the aglycone of ginseng saponin which is biologically active and considered to be the most important component of the Korean ginseng. Its properties : surface tension, critical micelle concentration (CMC), eye irritation, sorption onto hair, force reduction(%) and moisture retention effect were studied. Its cationic character allows the molecule to be more substantive than ginseng saponin. HGSQ had good physical properties and was safe. enough as a cosmetic raw material. Also half-head tests of HGSQ-containing shampoo were carried out to compare the conditioning effects in shampoos. HGSQ was an excellent conditioning agent in shampoo..

• 한국정밀공학회지
• /
• 제25권8호
• /
• pp.65-71
• /
• 2008

This paper presents a transient heat transfer analysis of a drum brake shape. The transient heat transfer analysis of automotive drum brakes with frictional contact is performed by using the finite element method. The drum brake type studied in the page is the internally expanding one in which two shoes fitted externally with frictional material are forced outward against surface a rotating drum on the wheel unit. In this case, the braking power is produced by the friction force between a drum and a lining, and is converted into heat. The brake drum has constant material properties. The air inside the drum has temperature-dependent thermal conductivity and enthalpy. Radiation effects are ignored. The result explains the reason why hair crack and cause of drum failure occur. The temperature of drum is in proportion to the drum thickness and nonlinear changes at every points of drum. It's necessary for the decrease of the drum temperature to make the air inside drum flow.

• 한국환경과학회지
• /
• 제17권7호
• /
• pp.809-816
• /
• 2008

The oriental ore minerals, which mainly consisted of talc, actinolite, sericite, and halloysite were developed, and then used to examine the healing effect on the skin wound in rats. Full-thickness square wounds were formed on the backs of rats after the hairs on the dorsal surface were shaved. The ore minerals were applied to examine the healing effect from day 0 to 15 after wounding. Notable wound healings in terms of congestion around the wound, wound contraction and epithelialization were found in ore mineral-treated groups. Moreover, microscopic results revealed the formation of epithelial layer, hair follicles and progressive angiogenesis in ore mineral-treated groups, while complete epithelial layer could not be found in the control. These results suggest that ore minerals from Korean indigenous ores may have wound healing effect on the skin injury in rats.

• Design & Manufacturing
• /
• 제10권3호
• /
• pp.25-29
• /
• 2016

Recently, a hyaluronic acid micro needle patch for therapeutic and cosmetic purposes has been used by attaching directly to the skin with a pattern having a micro needle shape of 1/3 thickness of hair. These products are attracting attention as an innovative product that maximizes the effect by activating the active ingredient in the skin in the deep skin without blocking the horny layer because the micro needle shape exists on the patch surface so that it can penetrate effectively to the skin. Currently, DAB (droplet air blowing) or MEMS technology is used to make pattern shapes for patches. Because of this technology, manufacturing time is long and manufacturing cost is high, so we tried to develop the mold technology to machine the microneedle shape directly to the metal. In this study, we first fabricated a needle pattern with a quadrangular pyramid shape and finally produced a conical needle pattern.