• 한국작물학회지
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• 제50권2호
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• pp.73-78
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• 2005

Demand for the domestic hairy vetch seed production will be increased with the increasing interest of environment-friendly agriculture in Korea. This study was conducted during from 2000 fall to 2003 spring at upland field of National Institute of Crop Science in Suwon, Korea to compare wheat and triticale (TC) as stake crop of hairy vetch (HV), and to know proper seeding rates and ratios between TC and HV for the maximum HV seed production. As supporting crop of HV, TC was superior to wheat at the points of higher HV seed yield, stronger TC stalk for supporting, consistence of ripening stage of two seeds. In seeding method, row seeding was superior to broadcast seeding at the points of less lodging and higher HV seed yield. HV seed yield decreased with the increase of TC seeding rate in mixture cropping (row seeding), particularly at TC seeding rates over 5kg/10a. HV seed yield increased with the increase of HV seeding rate at the condition of TC seeding rates under 5kg/10a in spite of higher lodging of mixed crops at higher HV seeding rate due to higher HV aboveground dry matter. Maximum HV seed yield was obtained at TC seeding rate of $1\~1.5kg/10a$ as indicating HV seed yield 176kg/l0a (CV. Madison) at seeding rate of TC 1kg/10a + HV 2 kg/10a in 2001, and HV seed yield 96kg/10a (CV. Common) at seeding rate of TC 1.5kg/10a + HV 4.5kg/10a in 2003. Use of all-purpose combine harvester for harvesting and appliance for separation of mixed seeds using centrifugal force, which are prerequisite for HV seed production, was excellent in the simultaneous seeds production system of HV and TC.

• 한국토양비료학회지
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• 제50권3호
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• pp.187-194
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• 2017

This study was conducted to investigate rice yield and soil chemical properties affected by incorporation levels of green barley (GB) and hairy vetch (HV). The GB and HV were applied to the pots at 500, 1,000, 1,500, and $2,000kg\;10^{-1}$ (GB500, GB1000, GB1500, GB2000, HV500, HV1000, HV1500, and HV2000, respectively), and inorganic fertilizer ($N-P-K=9-4.5-5.7kg\;10a^{-1}$) treatment as control. After rice harvesting, chemical properties of soil such as pH, OM, T-N, and available $P_2O_5$ in GB and HV treated treatments were improved over those in Control treatment. The rice yield ranged from 433 to $512kg\;10a^{-1}$ for GB treatments and 490 to $532kg\;10a^{-1}$ for HV treatments, indicating that rice yield was affected by incorporation levels of GB and HV. The rice yields in GB2000, HV1000, HV1500 and HV2000 treatments increased by 3.3, 3.1, 6.4 and 7.4% compared with Control treatment, respectively. Therefore, minimum incorporation level of GB and HV for increasing rice yield was $2,000kg\;10a^{-1}$ of GB and $1,000kg\;10a^{-1}$ of HV.

• 전기전자학회논문지
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• 제24권1호
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• pp.132-139
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• 2020

공기청정기에 사용되는 이오나이저 모듈은 권선형 transformer를 사용하여 방전전극인 HV+/HV-에 3.5KV/-4KV의 고전압을 공급하여 carbon fiber brush의 전계 방사에 의해 양이온과 음이온을 발생시킨다. 기존의 MCU를 이용한 이오나이저 모듈 회로는 PCB 사이즈가 크고 가격이 비싼 단점이 있고, 기존의 ring oscillator를 이용한 게이트 구동 칩은 oscillation 주기가 PVT(Process-Voltage-Temperature) 변동에 민감하고 HV+와 GND, HV-와 GND의 단락에 의한 fault detection 기능이 없으므로 화재나 감전의 위험이 있다. 그래서 본 논문에서는 7bit binary UP counter를 이용하여 PVT 변동이 있더라도 oscillation 주기를 조절하여 HV+ 전압이 목표 전압에 도달하게 한다. 그리고 HV+와 GND 사이의 단락을 검출하기 위한 HV+ short fault detection 회로, HV-와 GND 사이의 단락을 검출하기 위한 HV- short fault detection 회로와 HV+가 과전압 이상으로 올라가는 것을 검출하기 위한 OVP(Over-Voltage Protection) 회로를 새롭게 제안하였다.

• 한국미생물·생명공학회지
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• 제26권6호
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• pp.537-544
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• 1998

3-HV 몰분율이 증대된 P(3HB-3HV)의 고농도 생산을 위하여 재조합 phbC유전자를 모균주에 재도입시킨 형질전환 Alcaligenes eutrophus AER5의 배양공학적 연구를 수행하였다. 상기 형질전환균주를 전구물질인 valerate가 10.0g/L첨가된 최소배지에서 2단계 배양한 결과 P(3HB-3HV)내의 3-HV 몰분율이 52.2 mol%로 모균주 A. eutrophus H16의 30 mol%에 비해 현저히 증가하였다. 이와 같은 몰분율의 증가는 phbC 유전자의 산물인 PHB synthase의 증폭과 밀접한 관련이 있음을 확인하였다. 2단계 회분배양시 fructose의 보충첨가의 영향을 검토하였으며, 적정농도인 10.0g/L의 fructose를 valerate와 같이 첨가할 경우 총균체량, p(3HB-3HV)의 축적농도, 그리고 축적율은 현저히 증가한 반면, 3-HV 몰분율은 다소 감소하는 경향을 보였다. $Mg^{2+}$ 이온은 P(3HB-3HV) 축적 에 매우 민감한 영향을 미쳤으며, 적정 농도로 판단되는 0.1 g/L일 경우 P(3HB- 3HV) 축적농도는 6.1 g/L, 그리고 3-HV 몰분율은 71.3 %로 현저히 증가하였다. 또한 적정 pH 조절제의 선정을 위하여 NaOH, NaOH와 (NH$_4$)$_2$SO$_4$의 혼합액, 그리고 NH$_4$OH을 비교 검토하였다. 2단계 배양법의 번거로움을 극복하고저 1단계 간헐적 유가식배양법을 검토한 결과, 배양 72시간 후 총균체량 19.2g/L, P(3HB-3HV) 축적농도 10.4 g/L, 그리고 3-HV 몰분율 35 mol%로 2단계 회분 배양법보다 우수한 결과를 얻었다. 이는 형질전환균주를 이용한 3-HV 몰분율이 높은 P(3HB-3HV)의 고농도 생산을 위한 배양조건 확립을 위한 기초자료로 활용될 것이다.90배의 살충력을 보였다. SDS-PAGE와 Western blot 분석에서도 클론 pHLN2-72는 재조합 클론 pHLN2-80(-)보다 약간 높게 ICP가 생성이 되었었다. 이상의 결과는 과다발현에 Plac프로모터와 종결부위가 반드시 필요하며, -72 bp ICP 프로모터가 -80 bp 프로모터보다 과다발현률이 높았으며, ICP 유전자는 반드시 Plac프로모터의 전사 방향에 역방향으로 삽입이 되어야 하는 것으로 나타났다.사용함으로써 고품질의 전통주류 생산이 가능하다고 사료된다.noicacid, methylmalonic acid, 2-methyl-4-keto-pentan-2-ol 등과 지방산의 일종인 tetradecanoic acid, hexadecane, hept-adecanoic acid, octadecanoic acid 등이 확인되었다.er 7.6mm. Further rich management measure and investigation were recommended such as sapling protection, signboard construction, soil erosion controlling and regular monitoring within the community.ldom changed at level 1.will cause students to form the mathematical concepts correctly. 3. By visualizing the process of drawing the quadratic function graph, students understand the quadratic function graph structually. 4. Through the feedback, the recognition ability of the trigonometric function can be improved. 5. It is possible to change

• 분석과학
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• 제18권3호
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• pp.257-262
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• 2005

미토콘드리아 DNA(mtDNA) 상의 조절부위(control region)에는 HV1과 HV2와 같은 과변이부위(hypervariable region)가 있으며, 이 부위에서 사람마다 차이가 나는 많은 SNPs(single nucleotide polymorphism)을 발견할 수 있다. mtDNA 염기서열 분석은 개인식별 및 백골화된 시신등의 신원확인에 유용하게 사용되어왔다. mtDNA상의 cytochrome b(cytb) 유전자는 분자계통학(molecular phylogenetics) 분야에 널리 이용되고 있으며, 법과학 분야에서의 동물 종식별은 다양한 사건 현장 증거물의 인수식별 뿐 아니라 불법 유통되고 있는 각종 동물성 건강식품, 의약품의 원료 규명 및 보호 종의 밀렵 증명 등에 유용하게 적용될 수 있다. 본 연구에서는 광범위한 동물의 cytochrome b 유전자를 증폭할 수 있는 primer sets (H14724/L15149)와 사람에 특이적인 HV1 부위 primer set (H15997/L16236)를 이용한 동시 증폭을 통해 먼저 사람과 동물을 구별하였고, cytb 증폭산물의 직접 DNA 염기서열 분석을 통해 종식별을 수행하였다. H14724/L15149 primer pair는 닭과 오리를 제외하고 사람, 소, 돼지, 개, 고양이, 생쥐, 쥐의 cytb를 증폭할 수 있었으며, H14841/L15149 primer pair는 닭과 오리도 증폭할 수 있었다. 효모, 곤충 및 세균은 모두 증폭산물이 생산되지 않았으며, H15997/L16236의 경우 사람의 HV1만이 선택적으로 증폭되었다. 또한 실제 사건의 예에서와 같이 본 연구가 혈흔의 종식별에 매우 유용함을 보여주었다.

• 한국초지조사료학회지
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• 제18권1호
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• pp.43-48
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• 1998

No-tillage silage corn with legume hairy vetch(Vicia villosa Roth, HV) has renewed interest in supply of mineral N, soil erosion control at sloping land and weed control by cover of HV killed. This study was conducted to monitor concentration of soil mineral N ($NO_3^-$ -N + $NH_4^+$-N) and to find out variation of growth, yield and N uptake of silage corn according to quantity of HV cover; HV-removed, 1X-HV, 2X-HV at field of Crop Experiment Station in 1996. HV groM in early spring decreased the mineral N of soil depth 7.5 -22cm before corn seeding. But, killed HV cover increased the concentration of soil mineral N at surface soil (0-7.5cm) up to 45.4mglkg at early growth stage of corn. Dry matter(Dh4) of corn at harvest was lower in W-removed than in Okg FNlha. But DM and N uptake of corn at harvest were increased by quantity of HV-cover increasing liom HV-removed to 2X-HV. Hairy vetch could substitute N fertilizer for silage corn by N mineralized h m HV killed, but reduced early growth and N uptake of corn before silk by reducing soil mineral N of plow layer.

• Journal of Microbiology and Biotechnology
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• 제32권1호
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• pp.110-116
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• 2022

Polyhydroxyalkanoates (PHAs) are emerging as alternatives to plastics by replacing fossil fuels with renewable raw substrates. Herein, we present the construction of engineered Escherichia coli strains to produce short-chain-length PHAs (scl-PHAs), including the monomers 4-hydroxyvalerate (4HV) and 3-hydroxyvalerate (3HV) produced from levulinic acid (LA). First, an E. coli strain expressing genes (lvaEDABC) from the LA metabolic pathway of Pseudomonas putida KT2440 was constructed to generate 4HV-CoA and 3HV-CoA. Second, both PhaAB enzymes from Cupriavidus necator H16 were expressed to supply 3-hydroxybutyrate (3HB)-CoA from acetyl-CoA. Finally, PHA synthase (PhaC_Cv) from Chromobacterium violaceum was introduced for the subsequent polymerization of these three monomers. The resulting E. coli strains produced four PHAs (w/w% of dry cell weight): 9.1 wt% P(4HV), 1.7 wt% P(3HV-co-4HV), 24.2 wt% P(3HB-co-4HV), and 35.6 wt% P(3HB-co-3HV-co-4HV).

• 한국약용작물학회지
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• 제27권1호
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• pp.1-8
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• 2019

Background: This study was conducted to investigate the effects of fertilization with the application of mixed organic matter after cultivating green manure crops on the growth and saponin content of Codonopsis lanceolata Trautv. Methods and Results: Five type of cultivation of green manure crops were done: hairy vetch as a single seedling crop (HV), hairy vetch and rye as a mixture of seedling (HV/R), additional fertilization with mixed organic matter after cultivation of a seedling mixture (HV/R/MO), chemical fertilizer (CF), and a non-treated control (NT). The total nitrogen content was the highest in the HV/R/MO treatment, followed by the HV, CF, HV/R and NT treatments, respectively. Nitrogen fixation was higher in the single seedling treatment with hairy vetch (HV) than in the treatment with a mixture of hairy vetch and rye seedling (HV/R). Moreover, the growth of C. lanceolata was greatly increased by the application of additional fertilizer after treatment with the mixture of hairy vetch and rye seedlings. The HV/R/MO treatment produced the highest total lancemaside content among the treatments, followed by HV, HV/R, CF, and NT, respectively. Conclusions: It was verified that C. lanceolata experienced superior growth with the application of mixed organic matter as additional fertilizer after the cultivation of green manure crops, and the content of its major saponins, lancemasides were also increased by this treatment.

• Journal of Veterinary Science
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• 제22권4호
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• pp.51.1-51.10
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• 2021

Background: Malignant catarrhal fever (MCF) is a highly fatal lymphoproliferative disease of cattle, deer, bison, water buffalo, and pigs caused by the gamma-herpesviruses alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). Objectives: This study aimed to determine the prevalence of OvHV-2 in sheep, goats, cattle, and buffalo in Rawalpindi and Islamabad, Pakistan, by applying molecular and phylogenetic methods. Methods: Blood samples were aspirated from sheep (n = 54), goat (n = 50), cattle (n = 46) and buffalo (n= 50) at a slaughterhouse and several farms. The samples were subjected to heminested polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis of the OvHV-2 POL gene and the OvHV-2 ORF75 tegument protein gene. Results: The highest percentage of MCF positive samples was in sheep (13%), whereas goat, cattle, and buffalo had lower positive percentages, 11%, 9%, and 6.5%, respectively. Four OvHV-2-positive PCR products obtained from sheep samples were sequenced. The sequences obtained were submitted to the NCBI GenBank database (MK852173 for the POL gene; MK840962, MK852171, and MK852172 for the ORF75 tegument protein gene). Phylogenetic analysis revealed a close similarity of study sequences with those of worldwide samples. Conclusions: This study is the first cross-sectional study on the prevalence and molecular detection of OvHV-2 in apparently healthy cattle and buffalo that could be carrying OvHV-2 acquired from OvHV-2-positive sheep and goats. The results indicate that OvHV-2 is circulating in Pakistan. Further studies are needed to characterize OvHV-2 and elucidate further its prevalence.

• Journal of Pharmaceutical Investigation
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• 제27권3호
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• pp.189-198
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• 1997

The purpose of the present study was to investigate the effect of medium chain fatty acid salts, reported as enhancers in insulin nasal absorption, on the rectal absorption of insulin in rats. The serum glucose and remained insulin level in perfusate were measured after rectal recirculation of insulin with or without sod. laurate, sod. caprate and sod. caprylate in situ. The addition of sod. laurate or sod. caprate reduced serum glucose concentration considerably. Sod. caprate (1.0%) showed the greatest promoting effect on the decrement of serum glucose. Eudispert hv hydrogels containing insulin with medium chain fatty acid salts were, thereby, prepared and evaluated. The release rate of insulin from Eudispert hv hydrogels was reduced with an increase in the content of Eudispert hv, and was raised with increasing NaOH concentration. Ten percent Eudispert hv hydrogels were offered for the rectal administration of insulin. The addition of 1.0% sod. caprate reduced serum glucose concentration remarkably after rectal administration of 10% Eudispert hv hydrogels containing insulin. The level of glucose decrement was greater by 30% compared to subcutaneous administration of insulin solution. From the above findings, Eudispert hv hydrogels would be used as useful rectal delivery systems of insulin.