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http://dx.doi.org/10.4142/jvs.2021.22.e51

Molecular detection and characterization of ovine herpesvirus-2 using heminested PCR in Pakistan  

Riaz, Aayesha (Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University)
Dry, Inga (The Roslin Institute, University of Edinburgh)
Dalziel, Robert (The Roslin Institute, University of Edinburgh)
Rehman, Saif Ur (Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University)
Shah, Muhammad Ali (Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University)
Akhtar, Hafiz Muhammad Naeem (Department of Parasitology and Microbiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University)
Yousaf, Arfan (Department of Clinical studies, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University)
Baig, Ruqia (Department of Zoology, Faculty of Sciences, PMAS-Arid Agriculture University)
Publication Information
Journal of Veterinary Science / v.22, no.4, 2021 , pp. 51.1-51.10 More about this Journal
Abstract
Background: Malignant catarrhal fever (MCF) is a highly fatal lymphoproliferative disease of cattle, deer, bison, water buffalo, and pigs caused by the gamma-herpesviruses alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). Objectives: This study aimed to determine the prevalence of OvHV-2 in sheep, goats, cattle, and buffalo in Rawalpindi and Islamabad, Pakistan, by applying molecular and phylogenetic methods. Methods: Blood samples were aspirated from sheep (n = 54), goat (n = 50), cattle (n = 46) and buffalo (n= 50) at a slaughterhouse and several farms. The samples were subjected to heminested polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis of the OvHV-2 POL gene and the OvHV-2 ORF75 tegument protein gene. Results: The highest percentage of MCF positive samples was in sheep (13%), whereas goat, cattle, and buffalo had lower positive percentages, 11%, 9%, and 6.5%, respectively. Four OvHV-2-positive PCR products obtained from sheep samples were sequenced. The sequences obtained were submitted to the NCBI GenBank database (MK852173 for the POL gene; MK840962, MK852171, and MK852172 for the ORF75 tegument protein gene). Phylogenetic analysis revealed a close similarity of study sequences with those of worldwide samples. Conclusions: This study is the first cross-sectional study on the prevalence and molecular detection of OvHV-2 in apparently healthy cattle and buffalo that could be carrying OvHV-2 acquired from OvHV-2-positive sheep and goats. The results indicate that OvHV-2 is circulating in Pakistan. Further studies are needed to characterize OvHV-2 and elucidate further its prevalence.
Keywords
Malignant catarrhal fever; DNA Viruses; Nested Polymerase Chain Reaction; Ruminants; Pakistan;
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