• Clinical and Experimental Pediatrics
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• 제50권12호
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• pp.1194-1199
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• 2007

목 적 : 본 연구자들은 전자간증, 부당 경량아, 자궁 내 발육지연 등의 질환들이 공통적으로 혈관내피세포의 기능 변화와 어느 정도 관련되어 있을 것으로 추론하였다. 따라서 전자간증 산모의 신생아, 부당 경량아의 제대혈관내피세포를 배양하여 제대의 혈관내피세포에서 발현되는 대표적인 혈관 수축작용 물질인 endothelin-1의 양을 측정한 후 정상 신생아 제대혈관 내피세포의 결과와 비교하여 각 질환에서 태아의 혈관내피세포의 endothelin-1 발현 기능에 어떤 차이가 있는지를 확인하고, 또한 배양된 정상아 제대혈관내피세포에 전자간증 산모의 신생아와 부당 경량아 및 정상아의 제대혈 혈청을 투여하여 발현된 endothelin-1의 양을 비교하여 혈청 내에 endothelin-1의 발현을 촉진시키는 인자가 있는지를 알아보았다. 방 법 : 전자간증으로 진단 받은 산모의 신생아를 전자간증군(n=7), 부당 경량아로 진단받은 신생아를 부당 경량아군(n=8), 정상 신생아를 정상군(n=12)으로 나누었다. 각 그룹에서 태어난 신생아 제대와 제대혈을 모아 제대혈은 그 혈청을 영하 $70^{\circ}C$ 이하에 보관하였다. 전자간증군, 부당 경량아군 및 정상군 신생아의 제대 혈관내피세포를 분리하고 배양하였다. 배양된 각각의 제대 혈관내피세포의 상층액에서 endothelin-1 level을 측정하였다. 다음에는 전자간증군, SGA군 및 정상군의 제대혈 혈장과 정상 신생아 제대 혈관내피세포를 함께 부양하여 그 상층액에서 endothelin-1 level을 측정하였다. 결 과 : 전자간증군, 부당 경량아군 및 정상군의 제대혈관내피세포를 배양하여 발현된 endothelin-1의 양을 측정한 결과 전자간증군과 SGA군의 제대혈관내피세포의 endothelin-1 발현은 정상군과 유의한 차이가 없었다. 그러나 배양된 정상아 제대혈관내피세포에 전자간증군과 SGA군의 제대혈 혈청을 투여하여 발현된 endothelin-1의 양은 정상군에 비하여 유의하게 증가되었다. 결 론 : 전자간증 신생아나 부당 경량아의 혈관내피세포의 기능이상을 유발하는 인자가 전자간증과 부당경량아 제대 혈청내에 존재할 가능성이 있을 것으로 생각된다.

• 대한한방내과학회지
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• 제29권2호
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• pp.490-499
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• 2008

Objectives : This study was aimed to elucidate the effects of Trichosanthes kirilowii extract (TKE) on the angiogenesis and growth of tumor cells. Methods : Tube formation assay was performed by using human umbilical vein endothelial cells (HUVEC), and anchorage dependent colony assay was performed by using B16-F10 melanoma, Hep G2 and HT1080, CT-26 and SNU-1 cells. Results : For HUVEC, TKE at a level of more than 100 ${\mu}g/m{\ell}$ suppresses cell growth. For HUVEC at 100 ${\mu}g/m{\ell}$ and greater TKE density, the formation of tubes was suppressed in a dose-dependant manner. TKE controls the colony formations of B16-F10 melanoma cells, CT 26 cells, and Hep G2 cells, and its effect is proportional to density. In HT1080 cells and SNU-1 cells, formation is suppressed regardless of density. Conclusions : From these results, it could be concluded that TKE has significant properties on anti-angiogenesis and growth inhibiting of tumor cells. It is suggested that TKE will be a good candidate for new drugs or therapeutics for anti-angiogenesis.

• 동의생리병리학회지
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• 제18권4호
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• pp.990-994
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• 2004

Cancer is an intractable disease for humans to overcome. Recently natural products or Oriental prescriptions have been on the spotlight to develop anticancer agents with little side-effects and good efficacy. KamikeKyuktang has been used for the treatment of cancer in Oriental medicine. However, its anti-cancer mechanism still remains unclear. KMKKTE is an ethanol extract of KamikeKyuktang composed of 12 medicinal herbs. Anti-proliferative effects of KMKKT was investigated on Lewis lung carcinoma cell (LLC) and A549 (human lung cancer cells). Half-maximal inhibition of the LLC and A549 cell proliferation by KMKKTE was found approximately 125㎍/㎖ and 250㎍/㎖, respectively. It also effectively inhibited the proliferation of HUVEC cells treated by bFGF and VEGF up to 30% of control at 125㎍/㎖ and the cell migration to 80% at 25 ㎍/㎖ in concentration dependent manner. Tube formation of HUVEC cells on matrigel also was significantly suppressed from 25㎍/㎖ of KMKKTE. Taken together, these results demonstrate that KMKKTE has antiangiogenic activity and be applied to angiogenesis dependent cancers.

• 대한한의학회지
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• 제43권3호
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• pp.106-121
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• 2022

Objectives: The purpose of this study was to investigate the anti-inflammatory effect of Cornus Officinalis fruit extract(CE) and Cornus Officinalis Fruit Cheonghyeol Plus(CCP) in Human Umbilical Vein Endothelial Cell. Methods: We measured cell viability of CE, CCP and treated HUVEC with TNF-α. We measured the mRNA expression levels of KLF2, eNOS, MCP-1, ICAM-1, VCAM-1, the protein expression levels of KLF2, eNOS, MCP-1, ICAM-1, VCAM-1, and the protein phosphorylation level of ERK, JNK, p38 and the biomarker expression levels of MCP-1, ICAM-1, VCAM-1. Results: 1.CE incresed the mRNA, protein expression levels of KLF2, eNOS at concentrations of 100㎍/㎖ compared to the control group. CE decresed the mRNA, protein and biomarker expression levels of MCP-1,ICAM-1,VCAM-1 at concentrations of 100㎍/㎖ compared to the control group. CE decresed the protein phosphorylation level of p38 at concentrations of 100㎍/㎖ compared to the control group. 2. CCP incresed the mRNA, protein expression levels of KLF2, eNOS at concentrations of 100㎍/㎖ or more compared to the control group. CCP decresed the mRNA, protein and biomarker expression levels of MCP-1, ICAM-1, VCAM-1 at concentrations of 100㎍/㎖ or more compared to the control group. CCP decresed the protein phosphorylation level of ERK at concentrations of 100㎍/㎖ or more, p38 at concentrations of 200㎍/㎖ or more, and JNK at concentrations of 400㎍/㎖ compared to the control group. Conclusions: These results present that CE and CCP has anti-inflammatory effect in HUVEC. So, it could help treat or prevent inflammation in vein caused by dyslipidemia and contribute prevention of cardiovascular and cerebrovascular cerebrovascular diseases.

• 한국식품영양학회지
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• 제29권6호
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• pp.998-1007
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• 2016

본 연구에서는 상추 추출물이 혈관내피세포의 염증반응에 미치는 영향 및 지질대사 개선 효과를 확인하기 위해 HUVEC을 이용한 in vitro 실험 및 고콜레스테롤혈증 모델을 이용한 동물실험을 수행하였다. 상추 추출물은 HUVEC에서 TNF-${\alpha}$에 의해 증가되는 세포접착단백질 ICAM-1, VCAM-1의 발현을 억제하였고 TNF-${\alpha}$에 의해 촉진되는 HUVEC와 단핵구 세포의 부착을 감소시켰다. 또한 상추 추출물은 TNF-${\alpha}$에 의해 증가되는 염증성 사이토카인 IL-6, IL-8 및 동맥경화 유발 케모카인 MCP-1의 생성을 농도 의존적으로 억제하였다. 고콜레스테롤혈증 동물모델에서 고콜레스테롤 식이군 및 상추 추출물이 첨가된 고콜레스테롤 식이군의 체중증가량 및 식이섭취량은 유의적인 차이를 보이지 않았다. 고콜레스테롤 식이군의 혈중 총 콜레스테롤 함량은 정상 식이군에 비해 크게 증가하였고 상추추출물이 첨가된 고콜레스테롤 식이군의 콜레스테롤 함량은 유의적으로 감소하였다. 고콜레스테롤 식이군의 혈중 중성지방 함량은 정상 식이군에 비해 약간 감소하였지만 유의적인 차이는 보이지 않았다. 상추 추출물이 첨가된 고콜레스테롤 식이군의 경우 혈중 중성지방 함량은 고콜레스테롤 식이군과 비교하였을 때 유의적인 차이를 보이지 않았다. 고콜레스테롤 식이군의 HDL-콜레스테롤 함량은 정상 식이군에 비해 감소하였고 상추 추출물이 첨가된 경우 HDL-콜레스테롤 함량은 고콜레스테롤 식이군에 비해 유의적으로 증가하였다. LDL-콜레스테롤 함량은 고콜레스테롤 식이군에서 크게 증가하였고 상추 추출물이 첨가된 경우 감소하였다. 혈청의 동맥경화지수 및 심혈관지수는 고콜레스테롤 식이군에서 정상 식이군에 비해 유의적으로 증가하였고 상추 추출물이 첨가된 고콜레스테롤 식이군에서 유의한 감소를 나타내었다. 이상의 연구결과는 상추 추출물이 혈관내피세포의 염증반응을 억제하여 혈관협착을 방지할 수 있고 혈중 지질대사의 개선 효과를 보여 혈액순환개선 및 동맥경화, 심혈관질환의 예방과 치료에 효과가 있다는 것을 의미한다.

• Food Science and Biotechnology
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• 제17권1호
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• pp.38-45
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• 2008

Antioxidant and anti-rheumatoid activities of Cirsium japonicum leaf extract (CJLE) were investigated in this study. CJLE had similar DPPH radical scavenging activity and reducing power to ascorbic acid and several flavonoids. Rheumatoid arthritis (RA) is a chronic inflammatory tissue-destructive disease, partly related with functions of hyaluronidases (HAases) and collgenases. CJLE ($1,000\;{\mu}g/mL$) had approximately 60.7 and 31.9% inhibition of HAase and collagenase activity, respectively. Also, CJLE inhibited lipopolysaccharide (LPS)-induced nitrite production in a dose-dependent manner, and CJLE ($1,000\;{\mu}g/mL$) suppressed approximately 70% of LPS-induced nitrite production effectively in RAW 264.7 macrophage cells. CJLE had inhibitory effects on the adherence of monocytic THP-1 to human umbilical vein endothelial cell (HUVEC) monolayers to the basal level. Inhibitory effect of CJLE on the adhesion was caused by suppression of tumor necrosis factor-a-upregulated expression of vascular cellular adhesion molecule-1 (VCAM-1) and E-selectin. We expect that CJLE may alleviate the inflammatory process in rheumatoid synovium, and these findings will raise the possibility of the usage of C. japonicum as a traditional pharmaceutical of anti-rheumatoid arthritis.

• International Journal of Oral Biology
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• 제39권3호
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• pp.131-136
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• 2014

Porphyromonas gingivalis is one of the most important periodontal pathogens and has been to known to invade various types of cells, including endothelial cells. The present study investigated the mechanisms involved in the internalization of P. gingivalis in human umbilical vein endothelial cells (HUVEC). P. gingivalis internalization was reduced by clathrin and lipid raft inhibitors, as well as a siRNA knockdown of caveolin-1, a principal molecule of lipid raft-related caveolae. The internalization was also reduced by perturbation of actin rearrangement, while microtubule polymerization was not required. Furthermore, we found that Src kinases are critical for the internalization of P. gingivalis into HUVEC, while neither Rho family GTPases nor phosphatidylinositol 3-kinase are required. Taken together, this study indicated that P. gingivalis internalization into endothelial cells involves clathrin and lipid rafts and requires actin rearrangement associated with Src kinase activation.

• Archives of Pharmacal Research
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• 제24권5호
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• pp.397-401
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• 2001

In the search for new naturally occurring angiogenic inhibitory we found that culture broths from two unidentified fungal strains exerted potent inhibitory activities on capillary-like tube formation of human umbilical vein endothelial cells (HUVEC) in vitro. Two active compounds were isolated by bioassay-guided separation and their structures were identified as gliotoxin (1) and its derivative methylthiogliotoxin (2) by spectroscopic analyses. These compounds significantly inhibited the migration of HUVEC assessed by in vitro wounding migration assay and exhibited at least 10 times more potent inhibition of proliferation of HUVECs as compared with that of cancer cell lines such as HeLa, MCF-7, and KB 3-1 cells. Especially, gliotoxin having disulfide group exerted more potent activities than methylthiogliotoxin, suggesting that gliotoxin could be a useful compound for further study as an anti-angiogenic agent.

• 환경생물
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• 제27권1호
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• pp.88-94
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• 2009

안토시아닌(Anthocyanin)은 플라보노이드계 화합물의 한 부류로 항산화, 항암 및 항궤양, 항당뇨, 중금속해독, 시력보호, 콜레스테를 저하 등의 다양한 생리활성을 가지는 것으로 보고되어 있다. 죽상경화과정은 염증성 사이토카인의 분비 또는 혈관손상으로 인한 백혈구의 부착과 이동을 통해 시작된다. 본 연구는 이러한 죽상경화의 초기과정에서 안토시아닌 혼합물 중 single compound인 delphinidin chloride (DC) 인간혈관 내피세포주(HUVEC, human umbilical vein endothelial cell line)에서 백혈구 부착과 관련이 있는 ICAM-1 (Intraceliular Adhesion Molecule-1)과 VCAM-1 (Vascular Adhesion Molecule-1) 발현에 미치는 영향에 대해 조사하였다. 세포독성이 없는 농도에서 TNF-$\alpha$에 의해 유도된 혈관 내피세포에 대한 단핵구의 부착정도를 측정하기 위해 monocyte-endothelial cell adhesion assay와 광학현미경을 이용한 형태학적 관찰을 한 결과 DC가 처리농도 의존적으로 부착을 억제하였다. 내피세포로부터 TNF-$\alpha$에 의해 유도된 세포부착 분자인 VCAM-1과 ICAM-1의 발현에 대한 영향을 western blot analysis 및 RT-PCR방법으로 비교 분석한 결과 VCAM-1과 ICAM-1의 단백질과 mRNA수준에서의 발현이 농도 의존적으로 감소되었다. 이러한 결과들을 종합해 볼 때 안토시아닌 중에서 DC를 실험한 결과 DE는 TNF-$\alpha$에 의해 유도된 내피세포의 ICAM-1과 VCAM-1 발현 억제효과를 확인할 수 있었다.

• 동의생리병리학회지
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• 제29권2호
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• pp.180-188
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• 2015

This study was conducted to investigate the relaxation effects and its mechanisms of Nelumbinis Semen(NS) extract in isolated rabbit corpus cavernous tissues. In order to examine the relaxation effects and its mechanisms of NS, we treated the ethanol extract of NS(0.01-3.0 mg/ml) and indomethacin(IM), tetraethylammonium chloride(TEA), Nω -nitro-L-arginine (L-NNA), methylene blue(MB) were treated before NS extract to contracted strips induced by PE 1 μM. We also treated calcium chloride(Ca) 1 mM after pretreatment of NS extract in Ca²⁺-free krebs-ringer solution to contracted strips induced by PE. Cell viability and NO concentration on human umbilical vein endothelial cell(HUVEC) was measured by MTT assay, Griess reagent system. eNOS production was investigated by histochemical and immunohistochemical staining. NS extract was significantly affected on the relaxation of cavernous strips and NS extract-induced relaxation was not different by pretreatment of IM, TEA, MB, but inhibited by the pretreatment of L-NNA. And increase of contraction induced by Ca²⁺ addition, in a Ca²⁺-free solution, was decreased by pretreatment of NS. NO concentration on HUVEC was increased. When NS extract was applicated on corpus cavernosum of penis(CCP) in SHR, ratio of smooth muscles to collage fibers by PE was decreased and formation of eNOS around helicine artery was increased. These results suggest that CCP relaxation effects of NS extract are shown by suppressing influx of extracellular Ca²⁺ through the production of NO and eNOS.