DOI QR코드

DOI QR Code

Anti-inflammatory Effect of Cornus Officinalis fruit extract and Cornus Officinalis Fruit Cheonghyeol Plus in Human Umbilical Vein Endothelial Cell

인간 제대정맥 내피세포에서 산수유와 산수유청혈플러스의 항염증효과

  • Jeong-hui Kim (Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Ho-ryong Yoo (Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • In-chan Seol (Dept. of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Yoon-sik Kim (Dept. of Internal Medicine, College of Korean Medicine, Daejeon University)
  • 김정희 (대전대학교 한의과대학 심계내과학교실) ;
  • 유호룡 (대전대학교 한의과대학 심계내과학교실) ;
  • 설인찬 (대전대학교 한의과대학 심계내과학교실) ;
  • 김윤식 (대전대학교 한의과대학 심계내과학교실)
  • Received : 2022.07.20
  • Accepted : 2022.08.16
  • Published : 2022.09.01

Abstract

Objectives: The purpose of this study was to investigate the anti-inflammatory effect of Cornus Officinalis fruit extract(CE) and Cornus Officinalis Fruit Cheonghyeol Plus(CCP) in Human Umbilical Vein Endothelial Cell. Methods: We measured cell viability of CE, CCP and treated HUVEC with TNF-α. We measured the mRNA expression levels of KLF2, eNOS, MCP-1, ICAM-1, VCAM-1, the protein expression levels of KLF2, eNOS, MCP-1, ICAM-1, VCAM-1, and the protein phosphorylation level of ERK, JNK, p38 and the biomarker expression levels of MCP-1, ICAM-1, VCAM-1. Results: 1.CE incresed the mRNA, protein expression levels of KLF2, eNOS at concentrations of 100㎍/㎖ compared to the control group. CE decresed the mRNA, protein and biomarker expression levels of MCP-1,ICAM-1,VCAM-1 at concentrations of 100㎍/㎖ compared to the control group. CE decresed the protein phosphorylation level of p38 at concentrations of 100㎍/㎖ compared to the control group. 2. CCP incresed the mRNA, protein expression levels of KLF2, eNOS at concentrations of 100㎍/㎖ or more compared to the control group. CCP decresed the mRNA, protein and biomarker expression levels of MCP-1, ICAM-1, VCAM-1 at concentrations of 100㎍/㎖ or more compared to the control group. CCP decresed the protein phosphorylation level of ERK at concentrations of 100㎍/㎖ or more, p38 at concentrations of 200㎍/㎖ or more, and JNK at concentrations of 400㎍/㎖ compared to the control group. Conclusions: These results present that CE and CCP has anti-inflammatory effect in HUVEC. So, it could help treat or prevent inflammation in vein caused by dyslipidemia and contribute prevention of cardiovascular and cerebrovascular cerebrovascular diseases.

Keywords

References

  1. Oh BH. (2003). New Concepts in the pathogenesis and Progression of Atherosclerosis. Medical postgraduates, 31(4), 179-183.
  2. Statistics Korea. (2021). 2020 Death statistics : Korea. Korea development institute. Available from: URL: http://kostat.go.kr/portal/korea/kor_nw/1/6/2/index.board?bmode=read&bSeq=&aSeq=403046&pageNo=1&rowNum=10&navCount=10&currPg=&searchInfo=&sTarget=title&sTxt=
  3. Jeong HS. (2019). Risks and Management of Dyslipidemia. Public health weekly report. 2019; 12(37): 1416-1211. Available from: URL: https://www.kdca.go.kr/board/board.es?mid=a20602010000&bid=0034&list_no=364839&act=view 10000&bid=0034&list_no=364839&act=view
  4. Bae JH, Park JS, Hond GR, Shin DG, Kim YJ, Shim BS. (2008). Correlation between inflammatory markers and the progression of atherosclerosis in patients with coronary artery disease. The Korean Journal of Medicine, 74(1), 51-58.
  5. Stenvinkel P. (2003). Interactions betwen inflammation, oxi dative stres, and endothelial dysfunction in end-stage re nal disease. J Ren Nutr, 13(2), 144-148. https://doi.org/10.1053/jren.2003.50018.
  6. Wolf D, Ley K. (2019). Immunity and Inflammation in Atherosclerosis. Circ Res, 124(2), 315-327. https://doi.org/10.1161/CIR CRESAHA.118.313591.
  7. Glass CK, Witztum JL. (2001). Atherosclerosis. The road ahead. Cell, 104(4), 503-516. https://doi.org/10.1016/s0092-8674(01)00238-0.
  8. Seo DH, Joo IH, Kim DH. (2018). Effect of ChungHuyl-Plus on inflammatory factors in Human Umbilical Vein Endothelial Cells (HUVECs). J Haehwa Medicine, 27(2), 11-20.
  9. Oh JM, Cho HK, Yoo HR, Kim YS, Seol IC. (2016). A Case Report of the Beneficial Effects of Chunghyul-Plus in Dyslipidemia Patients. The Journal of the Society of Stroke on Korean Medicine, 17(1), 55-66.
  10. Park EB, Kim HS, Shin SY, Ji IA, Kim JH, Kim SG, at al. (2021). Antioxidative Activity of Cornus officianalis Extracts Obtained by Four Different Extraction Techniques. Journal of Life Science, 22(11), 1507-1514. http://dx.doi.org/10.5352/JLS.2012.22.11.1507
  11. Kim YJ and Son DY. (2016). Antioxidant activity and suppression of pro-inflammatory mediator of Corni fructus extracts in activated. Korean J Food Preserv, 23(6), 876-882. https://doi.org/10.11002/kjfp.2016.23.6.876.
  12. Shin JH, Cha GY, Kim HJ, Hwang JH, Han KH, Seo HJ, et al. (2009). Exmination of Anti-Obesity Effect of RegionalSpecial Natural Products of Anthrisci radix, Psoraleaesemen, Siegesbeckiae herba and Corni fructus. KSBB Journal, 24(6), 549-555.
  13. Yamabe N, Noh JS, Park CH, Kang KS,Shibahara N, Tanaka T, et al. (2010). Evaluation of loganin, iridoid glycoside from Corni Fructus, on hepatic and renal glucolipotoxicity and inflammation in type 2 diabetic db/db mice. European Journal of Pharmacology, 648(13), 179-187. https://doi.org/10.1016/j.ejphar.2010.08.044. Epub 2010 Sep 15.
  14. Jiang ZQ, Li Y, Jiang LH, Gu H, Wang MY. (2013). Hepatoprotective effects of extracts from processed corni fructus against D-galactose-induced liver injury in mice. Zhong Yao Cai, 36(1), 85-89.
  15. Ross R. (1993). The pathogenesis of atherosclerosis: A perspective for the 1990s. Nature. 362(6423), 801-809. https://doi.org/10.1038/362801a0.
  16. Committee of Clinical Practice Guideline of the Korean Society of Lipid and Atherosclerosis (KSoLA). (2018). Korean Guidelines for the Management of Dyslipidemia 4th ed. Seoul. 31-44.
  17. Li JJ, Fang CH. (2004). Atheroscleritis is a more rational term for the pathological entity currently known as atherosclerosis. Med Hypotheses, 63(1), 100-102. https://doi.org/10.1016/j.mehy.2004.01.029.
  18. Bergheanu SC, Bodde MC, Jukema JW. (2017). Pathophysiology and treatment of atherosclerosis. Neth Heart J, 25(4), 231-242. https://doi.org/10.1007/s12471-017-0959-2.
  19. Yang YK. (1991). Hwangjenegyung-yuckhe. Seoul: Iljoong-sa.
  20. Yang JH, Yoo HR, Kim YS, Seol IC. (2021). The Effect of Lonicera Japonica Thunberg on Inflammatory Factor Expression Associated with Atherosclerosis. Korean J. Orient. Int. Med, 42(1), 25-39. https://doi.org/10.22246/jikm.2021.42.1.25.
  21. Do HJ, Kim KH, Oh TW. (2020). Anti-hyperlipidemic Effects of Scutellariae Radix, Aucklandiae Radix and Bupleuri Radix (SAB) extract in FL83B cells. Kor. J. Herbol, 35(5): 23-31. https://doi.org/10.6116/kjh.2020.35.5.23.
  22. Han BH, Yoon JJ, Kim HY, Ahn YM, Hong MH, Son CO, et al. (2018). Inhibitory Effects of Ojeoksan on TNF-α-induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells. Kor. J. Herbol, 33(4), 59-67. https://doi.org/10.6116/kjh.2018.33.4.59.
  23. Lee KW, Cho HK, Yoo HR, Seol IC. (2018). The Effects of an Extract of Fermented Artemisiae Iwayomogii Herba,Curcumae Longae, Crataegi Fructus and Salviae Miltiorrhizae Radix on Anti-inflammation Associated with Dyslipidemia and Anti-oxidation in RAW264.7 and HUVEC Cells. J. Int. Korean Med, 39(4), 480-494. https://doi.org/10.22246/jikm.2018.39.4.480.
  24. Choi KE, Seol IC, Kim YS, Jo HK, Yoo HR. (2016). Hypolipidemic and Anti-oxidant Effects of Chunghyl Plus in Type II Diabetic Mice Model. J Physiol & Pathol Korean Med, 30(3), 164-176. https://doi.org/10.15188/kjopp.2016.06.30.3.164
  25. Lee JH, Jang HJ, Kim KS, Yang HJ, Lee JY, Na YC, et al. (2014). Effects of β-sitosterol derived from Artemisia capillaris on the activated human hepatic stellate cells and dimethylnitrosamine-induced mouse liver fibrosis. BMC Complement Altern Med, 14(363), 1-10. https://doi.org/10.1186/1472-6882-14-363.
  26. Oh DY, Kang DS, Lee YG, Kim HS. (2019). Effects of Turmeric (Curcuma longa L.) on Blood Glucose and Lipid Metabolism Functional Improvement in STZ-induced Diabetic rats. J Environmental Science International, 28(5), 485-494. https://doi.org/10.5322/JESI.2019.28.5.485.
  27. Oh DY, Kim HS. (2019). Evaluation of Oxidation Inhibition and Nitrogen Oxide Scavenging Activity from Curcuma longa L. Extracts. J Kor. applied science and technology, 36(1), 13-22. https://doi.org/10.12925/jkocs.2019.36.1.13
  28. Kwon SH, Kim JB. (2010). Effects of Crataegii Fructus on the Diet-induced Hyperlipidemia in Rats. Korean J. Oriental Physiology & Pathology, 24(1), 67-73.
  29. Lee SE, Cho SI. (2015). Anti-inflammatory effects of Salviae Miltiorrhizae Radix extract on RAW264.7 cell. via anti-oxidative activities. Kor. J. Herbol, 30(4), 89-94. http://dx.doi.org/10.6116/kjh.2015.30.4.89.
  30. Zhang J, Liang R, Wang L, Yang B. (2019). Effects and mechanisms of Danshen-Shanzha herb-pair for atherosclerosis treatment using network pharmacology and experimental pharmacology. J Ethnopharmacol, 2019, 229(1), 104-114. https://doi.org/10.1016/j.jep.2018.10.004.
  31. Packard RRS, Libby P. (2008). Inflammation in atherosclerosis: from vascular biology to biomarker discovery and risk prediction. Clin Chem, 54(1), 24-38. https://doi.org/10.1373/clinchem.2007.097360.
  32. Onat D, Brillon D, Colombo PC, Colombo PC, Schmidt AM. (2011). Human Vascular Endothelial Cells: A Model System for Studying Vascular Inflammation in Diabetes and Atherosclerosis. Curr Diab Rep, 11(3), 193 -202. https://doi.org/10.1007/s11892-011-0182-2.
  33. Bu D, Tarrio M, Grabie N, Zhang Y, Yamazaki H, Stavrakis G, et al. (2010). Statin-induced Kruppel-like factor 2 expression in human and mouse T cells reduces inflammatory and pathogenic responses. J Clin Invest, 120(6), 1961-1970. https://doi.org/10.1172/JCI41384.Epub 2010 May 3.
  34. Boon RA, Horrevoets AJ. (2009). Key transcriptional regulators of the vasoprotective effects of shear stress. Hamostaseologie, 29(1), 39-43. https://doi.org/10.1055/s-0037-1616937
  35. Yim CY. (2010). Nitric oxide and cancer. Korean J Med, 78(4), 430-436.
  36. Shin JJ, Lee SY, Lee SH, Lee SH, Suh JK, Cho JY, et al. (1996). The Role and Localization of Nitric Oxide Synthase in Neurogenic Inflammation of the Rat Airways. Tuberculosis and Respiratory disease, 43(3), 420-432. https://doi.org/10.4046/trd.1996.43.3.420.
  37. Ramji DP, Davies TS. (2015). Cytokines in atherosclerosis: Key players in all stages of disease and promising therapeutic targets. Cytokine Growth Factor Rev, 26(6), 673-685. https://doi.org/10.1016/j.cytogfr.2015.04.003.Epub 2015 May 12.
  38. Kim EK, Choi EJ. (2015). Compromised MAPK signaling in human diseases: an update. Arch Toxicol, 89(6), 867-882. https://doi.org/10.1007/s00204-015-1472-2. Epub 2015 Feb 18.
  39. Ryu IH, Cho HB, Kim SB, Seo YJ. (2011). The inhibitory effect of Picrasmae Lignum on inflammatory responses. J Kor. Medicine Obstetrics and Gynecology, 24(1), 1-14. https://doi.org/10.15204/jkobgy.2011.24.1.001.