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Effects of Oocytes Maturation and Fertilization Time on In Vitro Production and Quality of Korean Native Cattle

  • 박용수;최수호;김재명;박흠대;변명대
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2002.11a
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    • pp.79-79
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    • 2002
  • 본 연구에서는 in vitro에서 성숙된 난자의 핵성숙(Polar Body extrusion)에 소요되는 시간과 배반포 단계로의 발달능력 사이의 관계를 비교하여 조기에 발달능력을 가진 embryo를 선발할 수 있는 IVP 체계를 개발하고자 하였으며 in vitro maturation(IVM)에 따른 first polar body(PB) 형성, IVM과 IVF 시간이 oocyte의 발달에 미치는 영향과 생산된 배반포의 세포수를 평가하였다. IVM은 TCM199 배양액을 사용하였고 in vitro fertilization(IVF)은 Fer -TALP용액을 사용하였으며 in vitro culture(IVC)는 CRlaa 배양액을 사용하여 2일까지는 0.3% BSA를 3일 부터는 10%FBS와 bovine oviduct epithelial cell을 첨가하여 배양하였다. IVM 시간에 따른 PB의 출현율은 0hr(0%), 6hr(0%), 12hr(0%), 14hr(8.7%), 16hr(40.5%), 18hr(48.0%), 20hr(65%), 22(68%) 그리고 24hr(74.5%)을 보였으며 IVM 시간에 따른 cleavage 및 8cell 발달율 사이에는 유의적인 차이가 없었으나 배반포(BL) 및 8cell에서 배반포로 발달률은 18시간(BL 31$\pm$6, BL/8cell 82 $\pm$5%)에서 가장 높게 나타났으며 24시간(BL 17$\pm$2, BL/8cell 60$\pm$8%)과 유의적인 차이를 보였다(P<0.05). IVC 7일째 배반포의 총세포수와 trophoblast(TE) 세포수는 IVM 18시간(mean$\pm$S.E.; total: 131.1$\pm$34.0, TE: 97.6$\pm$29.6)에서 24시간(total: 112.2$\pm$17.5, TE: 80.1$\pm$15.6)보다 유의하게 많은 것으로 나왔으나(P<0.05) 7일째의 inner cell mass(ICM) 숫자(18hr 33.5$\pm$12.8 vs 24hr 32.1$\pm$12.0)와 8일째 ICM, TE 그리고 총 세포수에는 유의성 있는 차이가 없었다. IVM 18시간에서 PB 형성과 8cell 발달률 사이에 높은 상관성을 보였고 배반포 및 8cell에서 배반포 단계로 높은 발달률을 보였으며 생산된 배반포의 TE 숫자와 총 세포수가 유의하게 많은 것으로 나타났다. 따라서 IVM 18시간 실시하였을 경우 보다 많은 세포수를 가진 배반포 발달 가능성이 높은 embryo를 조기에 선발 가능할 것으로 사료된다.

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Analysis of the Environmental Radiation Dose Measured at the University Located in Suwon City (수원시 소재의 한 대학 내 환경방사선량 측정 분석)

  • Lee, In-Ja;Jeong, Yu-Jin;Kim, Chung-Kyung;Kim, Hee-Sol;Yoon, Hyeo-Min;Park, Da-Jeong;Ko, Yo-Han
    • Journal of radiological science and technology
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    • v.37 no.3
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    • pp.233-238
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    • 2014
  • The amount of environmental radiation was measured in the indoor(basement) and outdoor(rooftop) of the two buildings of the University (the old building and the new building) in Suwon City, Gyeonggi Province. Measuring the environmental radiation dose from the first week of April 2013 to last week of March 2014, Twice a week, one year. The measured results were as follows. 1. The average annual environmental radiation of the two buildings were $0.169727{\mu}Sv/hr$. The lowest value was $0.0760{\mu}Sv/hr$ whereas the highest value was $0.3000{\mu}Sv/hr$. 2. The amount of environmental radiation dose was $0.176373999{\mu}Sv/hr$ in the old building and the amount of environmental radiation dose was $0.163052885{\mu}Sv/hr$ in the new building. The old building environmental radiation dose was higher then the new building. 3. The month of the lowest amount of environmental radiation dose in a year was November resulting in $0.152844{\mu}Sv/hr$ whereas May was found the month of the highest amount of environmental radiation dose showing with $0.197222{\mu}Sv/hr$. 4. The amount of environmental radiation dose in the indoor(basement) was $0.164649{\mu}Sv/hr$ and the amount of environmental radiation dose of the outdoor(rooftop) was $0.174779{\mu}Sv/hr$. And the outdoor(rooftop) was higher than the indoor(basement). 5. Depending on seasonal, the lowest amount of environmental radiation dose was in winter with $0.1632{\mu}Sv/hr$ while spring was the season with the highest amount of environmental radiation dose with $0.183846{\mu}Sv/hr$. The measured amount was high in the order of spring, summer, autumn, and winter.

Infiltration Rate of Some Upland Soils in Korea (우리나라 밭토양의 수분침투속도(水分浸透速度)에 관하여)

  • Jung, Y.S.;Ryu, K.S.;Im, J.N.
    • Korean Journal of Soil Science and Fertilizer
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    • v.13 no.1
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    • pp.1-6
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    • 1980
  • The infiltration rates of the upland soils on hill side slope were investigated in situ using rainulator of which rainfall intensity was 100mm/hr. The soil moisture profile after the water infiltration was compared with that calculated from Youngs' equation. The results obtained are as follows: 1. Time required for infiltration rate to reach constant during rainfall was 15 to 25 min. The infiltration rate measured after 30 min was considered to be final infiltration rate. 2. The final infiltration rates of clay soils were lower than 10mm/hr., loamy soils 10 to 20., coarse loamy soils 20 to 30, and sandy soils higher than 30mm/hr., respectively. 3. The saturated hydraulic conductivity of the surface soil of Samgag sandy loam was 0.47mm/min., Songjeong clay loam0.16 mm/min., and Jeonnam silty clay loam 0.14mn/min., respectively. 4. The soil moisture profile calculated from Young's equation was in close agreement with measured in situ.

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The Effect of Seasonal Rhythm on the Gentamicin Pharmacokinetics in Healthy Volunteers (계절리듬이 겐타마이신의 약물동태에 미치는 영향)

  • Choi, Jun Shik;Kim, Jin;Baek, Chae Sun;Do, Nam Yong;Kim, Sung Hwan;Park, Young Jin
    • Korean Journal of Clinical Pharmacy
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    • v.8 no.2
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    • pp.89-94
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    • 1998
  • Seasonal rhythmic changes in gentamicin pharmacokinetics was evaluated in 10 healthy male volunteers after single intravenous 80 mg administration of gentamicin at 9:00 a.m. during summer and winter. The mean terminal half-life and AUC of gentamicin were $3.56\pm0.14\;hr\;and\;25.03\pm2.84\;{\mu}g/ml{\cdot}hr$ in winter and $3.08\pm0.41\;hr\;and\;21.84\pm2.51\;{\mu}g/ml{\cdot}hr$ in summer. The mean total body clearance $(CL_t)$ and elimination rate constant $(k_{10})$ of gentamicin was $3.17\pm0.43\;L/hr,\;0.458\pm0.06\;hr^{-1}\;in\;winter\;and\;3.66\pm0.45\;L/hr,\;0.561\pm0.07\;hr^{-1}$ in summer, The mean volumn of distribution $(V_{dss})$ of gentamicin at steady state was $12.65\pm1.09$L in winter and $12.39\pm1.25$ L in summer. Serum concentrations of gentamicin in winter were increased significantly during 4-8 hr (p<0.05) compared to those of gentamicin in summer. The elimination rate constant $(k_{10})$ of gentamicin in winter was decreased significantly $(p<0.05)$ compared to that of gentamicin in summer. The mean volume of distribution at steady state $(V_{dss})$, AUC, mean total body clearance ($CL_t$) and terminal half-life of gentamicin in the winter were increased but were not significant. The mean intrasubject fluctuations in terminal half-life, AUC and $CL_t$ between winter and summer were 8.2, 11.0 and $6.0\%$ respectively.

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Development of Chip-harvester for Collecting Forest Biomass and an Analysis of Productivity and Cost of Operation (산림바이오매스 수집용 칩하베스터의 개발과 생산성 및 비용 분석)

  • Kim, Jae-Hwan;Park, Sang-Jun
    • Journal of Korean Society of Forest Science
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    • v.106 no.1
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    • pp.54-62
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    • 2017
  • This study was carried to develop the chip-harvester and to analysis the operation productivity and cost for effective collection and forwarding of forest biomass. Main target specification of chip-harvester is speed of 8km/h, maximum climbing capacity of $30^{\circ}$ and maximum load capability of 2000 kg. Body structure is articulate type to reduce turning radius. Driving equipment is six-wheel drive, and a rear wheel is tandem bogie type to increase grip force. As a result of the driving test about developed chip-harvester, driving speed was 6.9 km/hr and 8.1 km/hr in ${\pm}10%$ slope with loaded and 7.3 km/hr and 7.9 km/hr in ${\pm}10%$ slope without load. As a result of the operation productivity and cost, operation productivity of grinding and forwarding was approximately $10m^3$ per day, and operation cost was 393,126 won per day.

Statistical Analysis and Countermeasure about Fire Mistaken Dispatch (통계적 분석을 통한 화재 오인출동의 현황과 대책)

  • Eom, Sang-Yong;Kim, Kyoung-Jin;Lee, Su-Kyung
    • Fire Science and Engineering
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    • v.27 no.2
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    • pp.89-96
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    • 2013
  • In this research, the fire mistaken dispatch records for the past 11 years have been analyzed by using a statistic technique. Fire mistaken dispatch occurred yearly average $48,371{\pm}5,763$ time at 95 % confidence level. This is 1.2 times more than the dispatch for extinguishing the fire. Fire mistaken dispatch has been increased mainly by malfunctioning alarm among other various reasons and the reason of mistaken dispatch is different depending on the population of the region. In big cities, the burning smell accounts for the most of mistaken dispatch but in rural areas, the garbage incineration is the major reason for mistaken dispatch. As a result of calculating the mean time between fire mistaken dispatches (MTBFmd) by region, MTBFmd of Daejeon is 20.61 hr/time and its reliability is 95.26 % at t=1 hr. On the other hand, the MTBFmd of Gyeonggi is 0.58 hr/time and its reliability is 17.91 % at t=1 hr.

Alterations of Blood Cholinesterase Activity and Urinary Para-nitrophenol Excretion After Exposure to Organophosphorus Insecticides (유기인제 농약 폭로로 인한 혈중 Cholinesterase 활성치와 요중 P-nitrophenol의 배설량의 변동)

  • Lee, Byung-Kook;Chung, Kyou-Chull
    • Journal of Preventive Medicine and Public Health
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    • v.7 no.1
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    • pp.115-121
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    • 1974
  • In order to evaluate the health hazard due to exposure to organophosphorus insecticides, we measured the blood cholinesterase activity ana urinary para-nitrophenol among 56 exposed subjects. They are orchard workers, rice plant workers and smithion factory workers. The clinical symptoms were also checked by physicians. We also measured the blood cholinesterase activity and urinary para-nitrophenol excretion of 20 urban people and 15 rural people who had never been exposed to organophosphorus insecticides in order to compare them according to age, sex and geographical differences. And these results were also compared with those of exposed groups. The results obtained were as follows. 1. The normal plasma cholinesterase activity and cell cholinestrase activity were $0.861{\pm}0.148\;{\Delta}pH/hr$ and $0.822{\pm}0.154\;{\Delta}pH/hr$. And normal para-nitrophenol in urine was $1.21{\pm}0.52mg/liter$. 2. No significant difference was existed in blood cholinesterase activities and urinary para-nitrophenol excre tion according to sex, age and geographical difference. 3. The plasma cholinesterase activity and cell cholinesterase activity of orchard workers, rice plant workers and smithion factory workers were $0.682{\pm}0.189\;{\Delta}pH/hr,\;0.775{\pm}0.160\;{\Delta}pH/hr,\;0.754{\pm}0.123\;{\Delta}pH/hr,\;and\;0.691{\pm}0.082\;{\Delta}pH/hr,\;0.756{\pm}0.117\;{\Delta}pH/hr,\;0.739{\pm}0.117\;{\Delta}pH/hr$. And significant decreses in blood cholinesterase activities were existed among orchard workers and smithion factory workers compared with control group. 4, The urinary para-nitrophenol excretions of orchard workers, rice plant workers and smithion factory workers were $1.33{\pm}0.66mg/liter,\;1.19{\pm}0.88mg/liter\;and\;1.37{\pm}0.67mg/liter$ and there were no significant difference between exposed groups and control group. 5. The clinical symptoms complained during and after organophosphorus insecticides exposure were frequently ranked by headache (67.7%) and vertigo (64.5%) and muscular ataxia and weakness (51.6%).

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Settling Characteristics of Phosphorus in Nakdong Estuary Barrage (낙동강 하구호에서의 인의 침강특성)

  • YANG Jin-Woo;LEE Suk-Mo;PARK Chung-Kil
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.3
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    • pp.185-191
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    • 1990
  • A study on the characteristics of phosphorus sedimentation in Nakdong Estuary Barrage was conducted in June and July, 1989. Cylindric type sediment trap was designed for collecting of downward material. Downward flux and settling velocity of downward material were $296g/m^2/d$, 1.25m/hr in mid-layer and $955g/m^2/d$, 3.31m/hr in bottom-layer, respectively Downward flux and settling velocity of phosphorus were $0.64g/m^2/d$, 0.85m/hr in mid-layer and $1.97g/m^2/d$, 1.89m/hr in bottom-layer, respectively. Fractional composition of organic phophorus in downward material was $51.9\%$ in mid-layer water and $48.3\%$ in bottom-layer water. According to the result of this study, sedimentation of phosphorus plays an important role as a sinking effect in Nakdong Estuary Barrage.

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Corrosion and Oxidation Behaviors of ion-nitrided tool Steels (이온질화된 공구강 표면의 산화 및 공식거동)

  • Choe Han-Cheol;Lee Ho-Jong;Jeong Yong-Woon
    • Journal of Surface Science and Engineering
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    • v.38 no.3
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    • pp.126-135
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    • 2005
  • SKD 11 steel has been widely used for tools, metallic mold and die for press working because of its favorable mechanical properties such as high toughness and creep strength as well as excellent oxidation resistance. The ion nitrided tool steel containing Mo results in improvement of corrosion resistance, strength at high temperature and pitting resistance, especially in $Cl^-$ contained environment. But the Mo addition causes a disadvantage such as lower oxidation resistance at elevated temperature. In this study, several effects of ion-disadvantage on the oxidation characteristics for SKD 11 steel with various oxidation temperature were investigated. SKD 11 steels were manufactured by using vacuum furnace and solutionized for 1 hr at $1,050^{\circ}C$. Steel surface was ion nitrided at $500^{\circ}C$ for 1 hr and 5 hr by ion nitriding equipment. ion nitrided specimen were investigated by SEM, OM and hardness tester. Oxidation was carried out by using muffle furnace in air at $500^{\circ}C,\;700^{\circ}C\;and\;900^{\circ}C$ for 1hr, respectively. Oxidation behavior of the ion nitrided specimen was investigated by SEM, EDX and surface roughness tester. The conclusions of this study are as follows: It was found that plasma nitriding for 5 hr at $500^{\circ}C$, compared with ion nitriding for 1 hr at $500^{\circ}C$, had a thick nitrided layer and produced a layer with good wear, corrosion resistance and hardness as nitriding time increased. Nitrided SKD 11 alloy for 1hr showed that wear resistance and hardness decreased, whereas surface roughness increased, compared with nitrided SKD 11 alloy for 5 hr. The oxidation surface at $900^{\circ}C$ showed a good corrosion resistance.

Sericin and 4-hexylresorcinol combination ointment accelerates wound healing in the diabetic burn wound model

  • Kang, Yei-Jin;Jo, You-Young;Kweon, HaeYong;Kim, Seong-Gon
    • International Journal of Industrial Entomology and Biomaterials
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    • v.40 no.1
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    • pp.1-5
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    • 2020
  • Sericin has been used for the treatment of burn wound. The purpose of this study was to compare the wound healing between sericin plus 4-hexylresorcinol (4HR) ointment (SE+4HR) and base only ointment. Total 12 mice were included in this study. SE+4HR group showed significantly smaller wound size than base only group at 3 wk (P<0.05). Surface temperature was higher in SE+4HR group. In conclusion, SE+4HR group showed better wound healing than base only group.