• Title/Summary/Keyword: HO-TAM

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Optimization of gamma-Aminobutyric Acid Bioconversion by Recombinant Escherichia coli (재조합 Escherichia coli를 이용한 gamma-Aminobutyric Acid 전환 반응 최적화)

  • Le Vo, Tam Dinh;Hong, Soon-Ho
    • KSBB Journal
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    • v.27 no.2
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    • pp.127-130
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    • 2012
  • In this study, the effects of pH, temperature, IPTG concentration and substrate (MSG) concentration on gamma-aminobutyric acid (GABA) production in engineered Escherichia coli were investigated. Glutamate decarboxylase and glutamate/GABA antiporter were overexpressed in GABA aminotransferase knock-out strain for GABA production. The result of optimization study showed the GABA bioconversion was optimized at pH 3.5, $30^{\circ}C$, 0.5 mM IPTG, 10 g/L MSG. At this condition, 5.23 g/L of final GABA concentration of was achieved from 10 g/L of MSG, which corresponded to a GABA yield of 85.77%.

A Study on the Development of Robust Fault Diagnostic System Based on Neuro-Fuzzy Scheme

  • Kim, Sung-Ho;Lee, S-Sang-Yoon
    • Transactions on Control, Automation and Systems Engineering
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    • v.1 no.1
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    • pp.54-61
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    • 1999
  • FCM(Fuzzy Cognitive Map) is proposed for representing causal reasoning. Its structure allows systematic causal reasoning through a forward inference. By using the FCM, authors have proposed FCM-based fault diagnostic algorithm. However, it can offer multiple interpretations for a single fault. In process engineering, as experience accumulated, some form of quantitative process knowledge is available. If this information can be integrated into the FCM-based fault diagnosis, the diagnostic resolution can be further improved. The purpose of this paper is to propose an enhanced FCM-based fault diagnostic scheme. Firstly, the membership function of fuzzy set theory is used to integrate quantitative knowledge into the FCM-based diagnostic scheme. Secondly, modified TAM recall procedure is proposed. Considering that the integration of quantitative knowledge into FCM-based diagnosis requires a great deal of engineering efforts, thirdly, an automated procedure for fusing the quantitative knowledge into FCM-based diagnosis is proposed by utilizing self-learning feature of neural network. Finally, the proposed diagnostic scheme has been tested by simulation on the two-tank system.

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Empirical Study about Relationship between Factors Influencing Korean User's Intention to Use the Internet Banking Service (국내 인터넷 뱅킹 사용자의 이용의도에 영향을 미치는 요인간 관계에 관한 실증연구)

  • Lee, Kun-Chang;Chung, Nam-Ho;Lee, Jong-Shin
    • Asia pacific journal of information systems
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    • v.12 no.3
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    • pp.191-212
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    • 2002
  • Recently, Internet banking becomes a major trend in the financial marketplace. However, there are few researches to investigate relationship between factors influencing user's intention to use the Internet banking service. Therefore, this paper is aimed at analyzing the Internet banking service user's intention to use empirically. Our research framework is basically based on TAM(Technology Acceptance Model) proposed by Davis(1989). Empirical results using path analysis revealed that experience has a direct relationship with intention to use, while those three factors like perceived risk, self-efficacy, technical support indirectly affect intention to use.

The Effect of the Social Cognitive Factors on the Usage of SNS (SNS의 사회인지요인이 사용의도에 미치는 영향)

  • Son, Dal-Ho
    • The Journal of Information Systems
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    • v.23 no.3
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    • pp.73-97
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    • 2014
  • Internet has begun various transformation and innovation in terms of society, culture and industry. Today, Social Network Service(SNS) is an exceedingly popular and useful tool for making human relationships. Previous studies related to SNS, which based on Technology Acceptance Model(TAM), were focused on user acceptance of new technology. However, they had the limitations to focus on technology acceptance, without the consideration of the cognitive factors in using SNS. This study examined how the main factors of intention to use of SNS continuously affect perceived usefulness and ease of use through familiarity, self-expression, hedonic shopping value, utilitarian shopping value and personal relationship. The results of hypothesis testing showed that familiarity, self-expression, hedonic shopping value and personal relationship have some positive impacts on the perceived usefulness and ease of use. The practical contribution of this study is to suggest useful decision alternatives concerned to marketing strategies for acquiring and retaining long-term customers related to SNS business.

A Study on the Factors Affecting User's Intention to Use the Digital Convergence Device (Telematics) (디지털 융합제품(텔레매틱스)의 사용의도에 영향을 미치는 요인에 관한 연구)

  • Yoon, Sung-Hwan;Choi, Eun-Jee;Kang, Hyoung-Mo;Song, Gap-Ho;Gim, Gwang-Yong
    • 한국IT서비스학회:학술대회논문집
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    • 2007.05a
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    • pp.506-506
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    • 2007
  • 유비쿼터스 환경 하에서 새로운 첨단 핵심기술 및 서비스가 하나의 제품 안에 집중되어가는 시대적 요구가 대두되고 있다. 이에 부응하여 탄생한 컨버전스 제품인 텔레매틱스의 전망 역시 두드러지고 있다. 본 연구는 Davis[14]가 제안한 기술수용모델(Technology Acceptance Model:TAM)을 통하여 텔레매틱스 사용의도에 관한 수용모형을 제시하였다. 기술수용모델의 내부 요인인 사용의도, 인지된 유용성과 인지된 이용용이성 외에도 업체 신뢰도, 정황 인식성, 자기 효능감의 외부 요인으로 확장하여 요인 간의 구조적 관계를 위한 모형을 개발하였다. 실증분석을 한 결과 정황 인식성, 자기 효능감이 각각 인지된 유용성과 이용용이성에 영향을 미치는 것으로 나타났다. 이러한 결과를 토대로 텔레매틱스의 이용자에게 중요시 되는 특성에 대해 논의하였다.

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Major Criteria for Channel Selection in Banking Transaction

  • Cho, Nam-Jae;Park, Ki-Ho
    • Journal of Information Technology Applications and Management
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    • v.16 no.1
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    • pp.169-183
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    • 2009
  • The purpose of this research, based on the Media Selection Theory, the Technology Acceptance Model, and the Social Influence Theory, is to investigate the influential factors that affect media selection in banking transactions. Analyses showed that for location sensitive bank windows and ATMs(automatic teller machines), defined as offline-based transaction channels, convenience was the variable affecting media selection. However, in the case of online media not related to location, (phone banking, internet banking, and mobile banking) reliability was the significant variable influencing use. The findings show that banking organizations may benefit from identifying traits of media affecting use, and should differentiate customer services for competitive advantage.

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Development of Protein Secretion System using Type III Secretion System of Salmonella (Salmonella Type III Secretion System을 이용한 단백질 분비시스템 개발)

  • Dinh, Le Tam Vo;Hong, Soon-Ho
    • KSBB Journal
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    • v.24 no.4
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    • pp.393-396
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    • 2009
  • New protein secretion system was developed using Type III Secretion System of Salmonella. N-terminal region of SlrP and SptP effector proteins were fused with TliA and EstA-P lipases by overlapping PCR. Lipase activity of Salmonella with SptP-TliA fusion system increased by 2.6 fold compare with wild type Salmonella strain. This result showed that lipase secretion via the T3SS would be a useful protein secretion machinery.

The Study on Purchase Intention of the Mobile Environment (모바일 환경에서 구매의도에 영향을 미치는 요인)

  • Lim, Gyu-Hong;Lee, Jong-Ho
    • The Journal of Information Systems
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    • v.15 no.4
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    • pp.189-209
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    • 2006
  • The progress of mobile internet technologies has changed user access to information. The mobile internet allows to obtain contents and services from wireless networks via mobile devices. The purpose of this study is th find out important factors that influence on the customer purchase intention by mobile internet environment. Base on TAM(Technology Acceptance Model) and the latest literature, a comprehensive set of constructs and hypotheses were compiled with a methodology for testing them. We suggested five factors that effect purchase intention in mobile internet environment: Enjoyment Ease of use, Usefulness, Mobility, Trust To validate the research model, we collected data from cellular phone user using an online survey. The results of this study are as follows. First enjoyment ease of un, usefulness, and mobility had positive effects on trust. Second, Usefulness, ease of use, and trust had positive effects on purchase intention to use mobile internet. Third, enjoyment and mobility did not have positive effects on purchase intention to use mobile internet. Based on these results, we suggest marketing strategies.

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A Study of the Effects of Perceived Characteristics on Satisfaction and Continuous Usage Intention in Personal Communities (개인 커뮤니티의 지각된 특성이 만족 및 지속적 사용의도에 미치는 영향에 관한 연구)

  • Chung, Young-Soo;Jung, Chul-Ho
    • The Journal of Information Systems
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    • v.16 no.3
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    • pp.133-159
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    • 2007
  • The primary purpose of this study is to examine the effects of perceived characteristics on user satisfaction and continuous usage intention in personal communities. We developed a research model based on the literature reviews of personal communities, TAM, perceived risks, and satisfaction. The research model includes perceived playfulness, perceived ease of use, perceived usefulness, and perceived risk as perceived characteristics in personal communities. For validation of this theoretical model, we survey the users of 'Mini-hompy', one of the most popular personal communities in Korea. The research model was empirically verified by structural equation model analysis with data collected from 407 samples. Analysis of the results indicates that perceived ease of use is positively related perceived playfulness and perceived usefulness. Perceived playfulness, perceived ease of use, and perceived risks are significantly related to satisfaction. User's satisfaction has positive relationship with continuous usage intention in personal communities.

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A Concurrent Testing of DRAMs Utilizing On-Chip Networks (온칩네트워크를 활용한 DRAM 동시 테스트 기법)

  • Lee, Changjin;Nam, Jonghyun;Ahn, Jin-Ho
    • Journal of the Semiconductor & Display Technology
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    • v.19 no.2
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    • pp.82-87
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    • 2020
  • In this paper, we introduce the novel idea to improve the B/W usage efficiency of on-chip networks used for TAM to test multiple DRAMs. In order to avoid the local bottleneck of test packets caused by an ATE, we make test patterns using microcode-based instructions within ATE and adopt a test bus to transmit test responses from DRAM DFT (Design for Testability) called Test Generator (TG) to ATE. The proposed test platform will contribute to increasing the test economics of memory IC industry.