KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Development of Protein Secretion System using Type III Secretion System of Salmonella

Salmonella Type III Secretion System을 이용한 단백질 분비시스템 개발

  • Dinh, Le Tam Vo (School of Chemical Engineering & Bioengineering, University of Ulsan) ;
  • Hong, Soon-Ho (School of Chemical Engineering & Bioengineering, University of Ulsan)
  • ;
  • 홍순호 (울산대학교 생명화학공학부)
  • Published : 2009.08.29
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Abstract

New protein secretion system was developed using Type III Secretion System of Salmonella. N-terminal region of SlrP and SptP effector proteins were fused with TliA and EstA-P lipases by overlapping PCR. Lipase activity of Salmonella with SptP-TliA fusion system increased by 2.6 fold compare with wild type Salmonella strain. This result showed that lipase secretion via the T3SS would be a useful protein secretion machinery.

단백질을 동물세포내부로 직접 주입할 수 있는 살모넬라 균주의 T3SS를 활용하여 모델 단백질인 lipase를 세포외부로 분비하는 시스템을 제작하였다. T3SS의 effector 단백질인 SlrP, SptP의 N-말단부분과 lipase를 fusion하였다. Lipase 분비실험의 결과 S. typhimurium SL1344 (sptP-tliA) 균주의 경우 lipase 활성도가 약 2.6배 증가하는 결과를 얻을 수 있었다. 본 실험결과를 바탕으로 T3SS에 관한 연구가 더욱 활발히 이루어진다면, T3SS를 신개념 약물전달시스템으로 활용이 가능해 지리라 예상된다.

Keywords

References

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