• 대한한의학방제학회지
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• 제21권1호
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• pp.99-110
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• 2013

Objectives : This study was designed to investigate the effect of a herbal preparation HJ01 consisting of Salicornia herbacea, Citri Reticulatae Pericarpium, Crataegi Fructus and Glycyrrhizae Radix on adipocyte differentiation in OP9 cells and on poloxamer 407(P-407)-induced hyperlipidemia in mice. Methods : 1. MTT assay was used to evaluate the potential cytotoxicity of Salicornia herbacea, Citri Reticulatae Pericarpium, Crataegi Fructus, Glycyrrhizae Radix and HJ01, respectively. 2. Bone-marrow derived OP9 cells were treated with HJ01, and the alterations in fat storage in the cells were determined by the Oil red O assay. 3. The protein level of CAAAT/enhancer binding protein alpha($C/EBP{\alpha}$), as a adipocyte differentiation marker, was examined using western blot analysis in differentiated OP6 cells. 4. Adult male C57BL6 mice received intraperitoneal injections of P407 to induce hyperlipidemia, simultaneously, were treated with HJ01 for 4 weeks. Then the cholesterol (TC), triglyceride (TG) and high-density lipoprotein-cholesterol (HDL-c) levels in sera and liver tissues were measured. Results : 1. The MTT assay exhibited that Salicornia herbacea, Citri Reticulatae Pericarpium, Crataegi Fructus, Glycyrrhizae Radix and HJ01 showed no significant cytotoxicity in tested dosages. 2. Ten days' treatment with HJ01 markedly inhibited the increases in fat storage in differentiated OP6 cells. 3. Four weeks' treatment with HJ01 down-regulated the protein level of CAAAT/enhancer binding protein alpha($C/EBP{\alpha}$) but up-regulated the levels of adiponectin in differentiated OP9 cells. 5. HJ01 inhibited the accumulation of TC and TG in liver tissues and increased serum levels of TC in hyperlipidemic mice. Conclusions : These results suggest that HJ01 can in vitro inhibit adipocyte differentiation and fat storage in OP6 cells, in vivo improve the hyperlipidemia induced by P-407 in mice, which may be mediated by promoting glucose uptake and improving a lipid metabolite profile.

• 한국정보통신학회논문지
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• 제8권4호
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• pp.787-792
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• 2004

본 논문에서는 근거리무선 통신용 RF 모듈을 구성하는 핵심 부품인 5.5GHz 대역의 발진기를 설계 제작하였다. NEC사의 잡음 특성이 우수한 HJ FET인 NE3210S01를 사용하여 위상잡음 특성을 개선하였고, 구현된 회로는 HP사의 회로 시뮬레이터인 ADS2002를 사용하여 설계 및 제작하였으며 발진기의 특성을 측정한 결과, 중심 주파수 5.5 GHz에서 출력전력이 10 dBm 그리고 2차 고조파 억압이 -31 dBc이며 중심 주파수 100 kHz offset에서 -98.83 dBc의 위상잡음 특성을 얻었다. 제작된 발진기는 근거리 무선 통신용 국부 발진기로 이용될 수 있다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2004년도 춘계종합학술대회
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• pp.96-100
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• 2004

본 논문에서는 근거리무선 통신용 RF 모들을 구성하는 핵심 부품인 5.5GHz 대역의 발진기를 설계 제작하였다. NEC사의 잡음 특성이 우수한 HJ FET인 NE3210S01를 사용하여 위상잡음 특성을 개선하였고, 구현된 회로는 HP사의 회로 시뮬레이터인 ADS2002를 사용하여 설계 및 제작하였으며 발진기의 특성을 측정한 결과, 중심 주파수 5.5 GHz에서 출력전력이 10 ㏈m 그리고 2차 고조파 억압이 -31 ㏈c이며 중심 주파수 100 KHz offset에서 -98.83 ㏈c의 위상잡음 특성을 얻었다. 제작된 발진기는 근거리 무선 통신용 국부 발진기로 이용될 수 있다.

• 미생물학회지
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• 제45권1호
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• pp.69-73
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• 2009

단백질 분해효소는 아미노산 간에 존재하는 공유결합인 펩티드 결합을 절단하는 효소이며, 세계적으로 가장 많이 판매되는 효소이다. 해양 심층수로부터 분리된 Micrococcus sp. HJ19의 체외분비 단백질 분해효소를 생산하는 최적의 배지조건을 조사하기 위하여 기본배지로 변형 STY 배지(1% tryptone, 0.15% yeast extract, 0.01% NaCl, 여과살균한 바닷물)를 사용하였다. 탄소원으로 포도당보다 갈락토오스를 사용하였을 때 1.3배로 높은 활성을 보여주었으며, 질소원으로는 casamino acid를 사용하였을 때 가장 낮은 활성을 보여준 반면, 맥아추출물을 사용하였을 때 가장 높은 활성을 나타내었다. 생산된 체외단백질 분해효소는 $35^{\circ}C$에서 최적의 활성을 나타내었으며, 최적 pH는 8.5로 판명되었다.

• 한국콘텐츠학회논문지
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• 제16권1호
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• pp.151-157
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• 2016

구강인기방법과 지르코니아 블록 종류에 따른 지르코니아 코핑의 변연적합도 변화를 관찰하기 위해 2종의 고무인상재와 1종의 구강스캐너를 사용한 구강인기방법과 3종의 지르코니아 블록을 사용하여 지르코니아 코핑을 10개씩 제작하여 표면 거칠기와 변연적합도를 측정하였다. 표면 거칠기는 LUXEN Smile($2.3{\pm}0.9{\mu}m$) 블록이 가장 우수하였다. 그리고 협측 변연간격은 HL시편($26.5{\pm}2.1{\mu}m$), 설측 변연간격은 HL시편($27.2{\pm}2.1{\mu}m$), 근심 변연간격은 HJ시편($29.6{\pm}4.0{\mu}m$), 원심 변연간격은 HJ시편($29.0{\pm}3.0{\mu}m$)이 가장 낮게 나타났으며, 통계학적으로 유의차가 있었다(p<0.05). 이 결과, 구강인기방법과 지르코니아 블록 종류가 변연적합도에 영향을 준 것으로 나타났으며, 변연간격은 모든 군에서 임상적 허용 범위 내에 속했다.

• 한국정보통신학회논문지
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• 제8권7호
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• pp.1558-1564
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• 2004

본 논문에서는 근거리무선 통신용 RF 모듈을 구성하는 핵심 부품인 5.5GHz 대역 LNA와 마이크로스트립 패치 안테나를 설계하였다. LNA는 NEC사의 NE3210S01를 사용하여 잡음특성을 개선하였으며 마이크로 스트립 패치 안테나는 전방향성을 갖도록 설계하였다. 제작된 LNA는 이득이 약15dB, 입출력 반사손실이 -20 dB 이하이며, VSWR은 1.5를 넘지 않는 특성을 보였다. 단일패치 안테나는 입력 반사 손실이 -28 dB이며, VSWR은 1.2 이하의 우수한 특성을 나타내었다.

• 한국식품저장유통학회지
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• 제22권4호
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• pp.535-544
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• 2015

본 연구에서는 $\beta$-glucosidase활성이 있는 B. subtilis HJ18-9균을 접종하여 품종별로 청국장을 제조하여, 품종별 청국장의 품질특성과 isoflavone 함량 변화를 측정한 결과이다. 청국장의 단백질 분해 특유의 구수한 맛 성분을 유리하는 효소인 protease활성은 발효 전에는 새단백 청국장이 가장 높은 활성을 나타냈으며, 발효 후에는 우람 청국장이 가장 높은 protease활성을 나타냈다. 이는 구수한 맛 성분의 지표성분인 아미노태 질소함량과 같은 경향을 나타냈다. 환원당을 유리하는데 관여하는 a-amylase 활성은 새단백으로 제조한 청국장이 다른 품종에 비해 유의적으로 높은 함량을 나타냈으며 다섯가지 품종 모두 발효 후에 증가하는 경향을 나타냈다. 발효 후에 총 균수가 높아지는 경향을 나타냈으며 발효 초에는 우람 청국장이 유의적으로 높은 균수를 나타냈으나, 발효 후에는 품종 간에 유의적인 차이는 나타내지 않았다. 품종별 청국장의 isoflavone 함량은 발효초기에는 대풍, 우람 품종으로 제조한 청국장의 aglycone함량이 가장 높았으며, 48시간 발효 후에도 두 가지 품종이 각각 $1249.04{\pm}9.14ug/g$, $589.32{\pm}14.08ug/g$로 가장 높은 aglycone 전환율 나타냈다. 이는 발효 초 aglycone함량에 비해 5.31, 1.94배 증가시켰다.

• Journal of electromagnetic engineering and science
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• 제4권4호
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• pp.143-149
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• 2004

This paper describes characteristics of the single-balanced low IF resistive FET mixer for the digital beam forming(DBF) receiver. This DBF receiver based on the direct conversion method is designed with Low IF I and Q channel. A radio frequency(RF), a local oscillator(LO) and an intermediate frequency(IF) considered in this research are 1950 MHz, 1940 MHz and 10 MHz, respectively. Super low noise HJ FET of NE3210S01 is considered in design. The measured results of the proposed mixer are observed IF output power of -22.8 dBm without spurious signal at 10 MHz, conversion loss of -12.8 dB, isolation characteristics of -20 dB below, 1 dB gain compression point(PldB) of -3.9 dBm, input third order intercept point(IIP3) of 20 dBm, output third order intercept point(OIP3) of 4 dBm and dynamic range of 30 dBm. The proposed mixer has 1.0 dB higher IIP3 than previously published single-balanced resistive and GaAs FET mixers, and has 3.0 dB higher IIP3 and 4.3 dB higher PldB than CMOS mixers. This mixer was fabricated on 0.7874 mm thick microstrip $substrate(\varepsilon_r=2.5)$ and the total size is $123.1\;mm\times107.6\;mm$.

• Journal of electromagnetic engineering and science
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• 제2권2호
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• pp.117-123
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• 2002

This paper describes the design for the resistive FET mixer with low If for the 4-Ch DBF(Digital Beam Forming) receiver This DBF receiver based on the direct conversion method is generally suitable for high-speed wireless mobile communications. A radio frequency(RF), a local oscillator(LO) and an intermediate frequency(If) considered in this research are 2.09 GHz, 2.08 CHz and 10 MHz, respectively. This mixer is composed of band pass filter, a low pass filter and a DC bias circuit. Super low noise HJ FET of NE3210S01 is considered in design. The RE input power, LO input power and Vcs are used -10 dBm, 6 dBm and -0.4 V, respectively. In the 4-Ch resistive FET mixer, the measured If and harmonic components of 10 MHe, 20 MHz and 2.087 CHz are about -19.2 dBm, -66 dBm and -48 dBm, respectively The If output power observed at each channel of 10 MHz is about -19.2 dBm and it is higher 28.8 dBm than the maximum harmonic component of 2.087 CHz. Each If output spectrum of the 4-Ch is observed almost same value and it shows a good agreement with the prediction.

• Clinical and Experimental Reproductive Medicine
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• 제32권4호
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• pp.315-324
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• 2005

Objectives: To compare the efficacy of GnRH antagonist multiple dose protocol (MDP) with that of GnRH agonist long protocol (LP) in controlled ovarian hyperstimulation for in vitro fertilization in patients with high basal FSH (follicle stimulating hormone) level or old age, a retrospective analysis was done. Methods: Two hundred ninety four infertile women (328 cycles) who were older than 41 years of age or had elevated basal FSH level (> 8.5 mIU/mL) were enrolled in this study. The patients had undergone IVF-ET after controlled ovarian hyperstimulation using GnRH antagonist multiple dose protocol (n=108, 118 cycles) or GnRH agonist long protocol (n=186, 210 cycles). The main outcome measurements were cycle cancellation rate, consumption of gonadotropins, the number of follicles recruited and total oocytes retrieved. The number of fertilized oocytes and transferred embryos, the clinical pregnancy rates, and the implantation rates were also reviewed. And enrolled patients were divided into three groups according to their age and basal FSH levels; Group A - those who were older than 41 years of age, Group B - those with elevated basal FSH level (> 8.5 mIU/mL) and Group C - those who were older than 41 years of age and with elevated basal FSH level (> 8.5 mIU/mL). Poor responders were classified as patients who had less than 4 retrieved oocytes, or those with $E_2$ level <500 pg/mL on the day of hCG injection or those who required more than 45 ampules of exogenous gonadotropin for stimulation. Results: The cancellation rate was lower in the GnRH antagonist group than in GnRH agonist group, but not statistically significant (6.8% vs. 9.5%, p=NS). The amount of used gonadotropins was significantly lower in GnRH antagonist group than in agonist group ($34.8{\pm}11.3$ ampules vs. $44.1{\pm}13.4$ ampules, p<0.001). The number of follicles > 14 mm in diameter was significantly higher in agonist group than in antagonist group ($6.7{\pm}4.6$ vs. $5.0{\pm}3.4$, p<0.01). But, there were no significant differences in clinical pregnancy rate (24.5% in antagonist group vs. 27.4% in agonist group, p=NS) and implantation rate (11.4% in antagonist group vs. 12.0% in agonist group, p=NS) between two groups. Mean number of retrieved oocytes was significantly higher in GnRH agonist LP group than in GnRH antagonist MDP group ($5.4{\pm}3.5$ vs. $6.6{\pm}5.0$, p<0.0001). But, the number of mature and fertilized oocytes, and the number of good quality (grade I and II) and transferred embryos were not different between two groups. In each group A, B, and C, the rate of poor response did not differ according to stimulation protocols. Conclusions: In conclusion, for infertile women expected poor ovarian response such as who are old age or has elevated basal FSH level, a protocol including a controlled ovarian hyperstimulation using GnRH antagonist appears at least as effective as that using a GnRH agonist, and may offer the advantage of reducing gonadotropin consumption and treatment period. However, much work remains to be done in optimizing the GnRH antagonist protocols and individualizing these to different cycle characteristics.