Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Production Condition and Characterization of Extracellular Protease from Micrococcus sp. HJ-19

Micrococcus sp. HJ19에서 체외분비 단백질 분해효소의 생산조건과 효소특성

  • Cha, In-Tae (Department of Microbiology, Chungbuk National University) ;
  • Oh, Yong-Sik (Department of Microbiology, Chungbuk National University) ;
  • Cho, Woon-Dong (Department of Microbiology, Chungbuk National University) ;
  • Lim, Chae-Sung (Department of Microbiology, Chungbuk National University) ;
  • Lee, Je-Kwan (Department of Microbiology, Chungbuk National University) ;
  • Lee, Oh-Seuk (Department of Food Fermentation Technology, Young-Dong University) ;
  • Roh, Dong-Hyun (Department of Microbiology, Chungbuk National University)
  • 차인태 (충북대학교 자연과학대학 미생물학과) ;
  • 오용식 (충북대학교 자연과학대학 미생물학과) ;
  • 조운동 (충북대학교 자연과학대학 미생물학과) ;
  • 임채성 (충북대학교 자연과학대학 미생물학과) ;
  • 이제관 (충북대학교 자연과학대학 미생물학과) ;
  • 이오석 (영동대학교 와인발효식품학과) ;
  • 노동현 (충북대학교 자연과학대학 미생물학과)
  • Published : 2009.03.31
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Abstract

Proteases are degradative enzymes which hydrolyze a peptide bond between amino acids and they are abundantly applied to commercial field. In order to investigate optimal medium compositions of carbon and nitrogen source for enzyme production, modified STY medium containing 0.15% yeast extract were used as basal medium. When galactose was used as carbon source, enzyme activity showed 1.3 higher than that of glucose. For nitrogen source addition of casamino acids to basal medium in place of tryptone showed lowest activity, whereas addition of malt extract showed maximal activity. The optimum temperature and pH of extracellular protease were found to be $35^{\circ}C$ an pH 8.5.

단백질 분해효소는 아미노산 간에 존재하는 공유결합인 펩티드 결합을 절단하는 효소이며, 세계적으로 가장 많이 판매되는 효소이다. 해양 심층수로부터 분리된 Micrococcus sp. HJ19의 체외분비 단백질 분해효소를 생산하는 최적의 배지조건을 조사하기 위하여 기본배지로 변형 STY 배지(1% tryptone, 0.15% yeast extract, 0.01% NaCl, 여과살균한 바닷물)를 사용하였다. 탄소원으로 포도당보다 갈락토오스를 사용하였을 때 1.3배로 높은 활성을 보여주었으며, 질소원으로는 casamino acid를 사용하였을 때 가장 낮은 활성을 보여준 반면, 맥아추출물을 사용하였을 때 가장 높은 활성을 나타내었다. 생산된 체외단백질 분해효소는 $35^{\circ}C$에서 최적의 활성을 나타내었으며, 최적 pH는 8.5로 판명되었다.

Keywords

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