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Production Condition and Characterization of Extracellular Protease from Micrococcus sp. HJ-19  

Cha, In-Tae (Department of Microbiology, Chungbuk National University)
Oh, Yong-Sik (Department of Microbiology, Chungbuk National University)
Cho, Woon-Dong (Department of Microbiology, Chungbuk National University)
Lim, Chae-Sung (Department of Microbiology, Chungbuk National University)
Lee, Je-Kwan (Department of Microbiology, Chungbuk National University)
Lee, Oh-Seuk (Department of Food Fermentation Technology, Young-Dong University)
Roh, Dong-Hyun (Department of Microbiology, Chungbuk National University)
Publication Information
Korean Journal of Microbiology / v.45, no.1, 2009 , pp. 69-73 More about this Journal
Abstract
Proteases are degradative enzymes which hydrolyze a peptide bond between amino acids and they are abundantly applied to commercial field. In order to investigate optimal medium compositions of carbon and nitrogen source for enzyme production, modified STY medium containing 0.15% yeast extract were used as basal medium. When galactose was used as carbon source, enzyme activity showed 1.3 higher than that of glucose. For nitrogen source addition of casamino acids to basal medium in place of tryptone showed lowest activity, whereas addition of malt extract showed maximal activity. The optimum temperature and pH of extracellular protease were found to be $35^{\circ}C$ an pH 8.5.
Keywords
extracellular protease; medium compositions; Micrococcus sp. HJ19; optimal temperature & pH;
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