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Characteristics of the gene resources and selected strains of Agrocybe cylindracea (버들송이버섯(Agrocybe cylindracea)유전자원 및 선발계통의 특성)

  • Heo, Byong-Soo;Yoo, Young-Jin;Seo, Sang-Young;Choi, Kyu-Hwan;Choi, Young Min;Kwon, Seog-Ju;Jang, Kab-Yeul
    • Journal of Mushroom
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    • v.17 no.2
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    • pp.52-63
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    • 2019
  • Agrocybe spp. belongs to the Agaricales order, Bolbitiaceae family, and Agrocybe genus. In Korea, so far, it has been cultivated through bottle cultivation; therefore, this study was conducted for the development of a new cultivar using the bag cultivation method for quantitative improvement. Thirty-three gene resources of Agrocybe spp. were collected and their quantity and characteristics of bag cultivation were examined. Next, 5 kinds of crossing parents were selected based on the cultivation period and shape of the fruit body. Seven strains were selected through 3 cross combinations. The 7 selected strains and the comparative cultivar 'Cham' were cultivated in a bag. As a result, the cultivation period was 49 days for 'JBAC15-1' and 50 days for 'JBAC15-6' which are 4 days and 3 days less than the cultivation period of the comparative cultivar 'Cham'(53 days), respectively. Cultivation periods of other strains except for 'JBAC15-1' and 'JBAC15-1' were longer than that of the comparative cultivar'Cham'. The best ratio of primordia formation among the selected strains was found to be that of 'JBAC15-1' with 96.1% followed by 'JBAC15-6' with 94.5%. These rates were 3.1% and 1.5% higher than the ratio of primordia formation of the comparative cultivar 'Cham', which is found to be 93.0%. The quantity was maximum in the 'JBAC15-1' cultivar with 176.8 g per bag followed by 'JBAC15-6' with 168.7 g per bag. The quantities were 10% and 5% more than the comparative cultivar 'Cham' with 160.7 g per bag. Based on these results, 'JBAC15-1' and 'JBAC15-6', which had shorter cultivation periods and more quantities than the comparative cultivar 'Cham' were finally selected. For the selected strains of 'JBAC15-1' and 'JBAC15-6', mycelial growth was observed to be optimal on PDA medium and the optimum temperature was $27.5^{\circ}C$. The optimum pH was pH 5 for 'JBAC15-1' and pH 6 for 'JBAC15-6'. The color of the pileus of the fruit body was dark brown in 'JBAC15-1' and 'Cham' and light brown in 'JBAC15-6'. The pileus was hemispherical in shape in both 'JBAC15-1' and 'Cham'. However, the colors of the stem were different - light brown in 'JBAC15-1', white in 'JBAC15-6', and ivory in the comparative cultivar 'Cham'.

Estimation of Mean Surface Current and Current Variability in the East Sea using Surface Drifter Data from 1991 to 2017 (1991년부터 2017년까지 표층 뜰개 자료를 이용하여 계산한 동해의 평균 표층 해류와 해류 변동성)

  • PARK, JU-EUN;KIM, SOO-YUN;CHOI, BYOUNG-JU;BYUN, DO-SEONG
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.24 no.2
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    • pp.208-225
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    • 2019
  • To understand the mean surface circulation and surface currents in the East Sea, trajectories of surface drifters passed through the East Sea from 1991 to 2017 were analyzed. By analyzing the surface drifter trajectory data, the main paths of surface ocean currents were grouped and the variation in each main current path was investigated. The East Korea Warm Current (EKWC) heading northward separates from the coast at $36{\sim}38^{\circ}N$ and flows to the northeast until $131^{\circ}E$. In the middle (from $131^{\circ}E$ to $137^{\circ}E$) of the East Sea, the average latitude of the currents flowing eastward ranges from 36 to $40^{\circ}N$ and the currents meander with large amplitude. When the average latitude of the surface drifter paths was in the north (south) of $37.5^{\circ}N$, the meandering amplitude was about 50 (100) km. The most frequent route of surface drifters in the middle of the East Sea was the path along $37.5-38.5^{\circ}N$. The surface drifters, which were deployed off the coast of Vladivostok in the north of the East Sea, moved to the southwest along the coast and were separated from the coast to flow southeastward along the cyclonic circulation around the Japan Basin. And, then, the drifters moved to the east along $39-40^{\circ}N$. The mean surface current vector and mean speed were calculated in each lattice with $0.25^{\circ}$ grid spacing using the velocity data of surface drifters which passed through each lattice. The current variance ellipses were calculated with $0.5^{\circ}$ grid spacing. Because the path of the EKWC changes every year in the western part of the Ulleung Basin and the current paths in the Yamato Basin keep changing with many eddies, the current variance ellipses are relatively large in these region. We present a schematic map of the East Sea surface current based on the surface drifter data. The significance of this study is that the surface ocean circulation of the East Sea, which has been mainly studied by numerical model simulations and the sea surface height data obtained from satellite altimeters, was analyzed based on in-situ Lagrangian observational current data.

On the Persistence of Warm Eddies in the East Sea (동해 난수성 에디의 장기간 지속에 관하여)

  • JIN, HYUNKEUN;PARK, YOUNG-GYU;PAK, GYUNDO;KIM, YOUNG HO
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.24 no.2
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    • pp.318-331
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    • 2019
  • In this study, comparative analysis is performed on the long-term persisted warm eddies that were generated in 2003 (WE03) and in 2014 (WE14) over the East Sea using the HYCOM reanalysis data. The overshooting of the East Korea Warm Current (EKWC) was appeared during the formation period of those warm eddies. The warm eddies were produced in the shallow Korea Plateau region through the interaction of the EKWC and the sub-polar front. In the interior of the both warm eddies, a homogeneous water mass of about $13^{\circ}C$ and 34.1 psu were generated over the upper 150 m depth by the winter mixing. In 2004, the next year of the generation of the WE03, the amount of the inflow through the western channel of the Korea Strait was larger, while the inflow was lesser than its climatology during 2015 corresponding to the development period of the WE14. The above results suggest that the heat and salt are supplied in the warm eddies through the Tsushima Warm Current (TWC), however the amount of the inflow through the Korea Strait has negligible impact on the long-term persistency of the warm eddies. Both of the warm eddies were maintained more than 18 months near Ulleung island, while they have no common feature on the pathways. In the vicinity of the Ulleung basin, large and small eddies are continuously created due to the meandering of the EKWC. The long-term persisted warm eddies in the Ulleung Island seem to be the results of the interaction between the pre-existed eddies located south of the sub-polar front and fresh eddies induced by the meanderings of the EKWC. The conclusion is also in line with the fact that the long-term persisted warm eddies were not always created when the overshooting of the EKWC was appeared.

Study on improvement of USLE P factor considering topography and cultivation method (지형 및 경작 방법을 반영한 범용토양유실량 산정공식 보전관리 인자 개선 연구)

  • Sung, Yunsoo;Lee, Gwanjae;Lee, Gwanjae;Han, Jeongho;Kim, Jonggun;Lim, Kyoung Jae;Kim, Ki Sung
    • Journal of Wetlands Research
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    • v.21 no.2
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    • pp.163-172
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    • 2019
  • The USLE P factor is a factor that varies depending on how croplands are managed and cultivated. Previous studies tend to overestimate the amount of soil loss because the factor was estimated from the slope of the watershed rather than the estimate of each cultivated land. In addition, the accuracy of estimating the soil loss is decreasing due to the fact that the factor is calculated without considering various conditions of cultivated land defined by Wishmeier and Smith. In order to overcome these problems, the Ministry of Environment (MOE) has proposed to establish the topsoil notification and calculate the P factor according to the cultivation methods (e.g., tillage system, support practice). However, it is required to apply the conditions proposed in the United States to domestic circumstances as it is causing uncertainties. Thus, this study selected the watersheds where soil loss was serious (Haean, Jaun, Banbyeoncheon), measured the actual slopes and slope lengths, and examined the crop, tillage systems, and support practice for each cultivated land. The P factors were recalculated considering the actual conditions of cultivated land and compared to the factors proposed by the previous studies (MOE). As the result of the study, the P factors calculated based on the previous studies were 0.8 ~ 1.0 in three watersheds. On the other hand, it is confirmed that there is a significant difference between the factors notified by MOE and estimated by reflecting the topography and cultivation methods in this study. Therefore, it is considered that the research for developing the cultivation conditions to calculate the P factor suitable for the domestic environment should be continuously carried out.

A Study on the Decisive Factors Influencing the Career Preparation Activities of North Korean Adolescent Defector (탈북 청소년 진로준비행동에 영향을 미치는 결정요인에 관한 연구)

  • Cho, Hyun-Seob;Chae, Kyung-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.6
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    • pp.501-513
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    • 2019
  • In this study, which is based on the findings of the preceding studies, the researchers determined social support and Career Decision Self-efficacy as the key influencing variables for the successful Career Preparation Activities, to analyze the relationship between Career Preparation Activities and these variables, while identifying the mediating effect of Career Decision Self-efficacy in the relationship between social support and Career Preparation Activities. In addition, there is an objective to provide effective career counseling materials. For this study, a total of 174 North Korean adolescent defectors, who were in their middle and high schools, were interviewed and the resultant data were analyzed the data based on the questionnaire data of 204 copies from March 12 to 19, 2019. For the analysis of the data, SPSS and AMOS statistical suites were used to verify the hypothesis. The result of the analysis showed that, first, in the relationship between the social support, Career Decision Self-efficacy, and Career Preparation Activities of the North Korean adolescent defectors social support, Career Decision Self-efficacy, and Career Preparation Activities were all positively related. Especially, Career Decision Self-efficacy has been identified as the variable that is highly related to Career Preparation Activities. Second, rather than social support(${\beta}=.107$), Career Decision Self-efficacy(${\beta}=.388$) turned out to have more profound direct influence on Career Preparation Activities. Third, social support did not influence Career Preparation Activities directly(${\beta}=.107$, p>.05) but completely intermediated Career Decision Self-efficacy to influence Career Preparation Activities(Indirect effect=.307, p<.05, Z-value=2.924, p<.01). The findings of this study show that, in order to enhance the Career Preparation Activities of the North Korean adolescent defectors, it is necessary to examine how they perceive the emotional, informational, material, and evaluative supports from the surrounding environment are perceived and identify the abilities, values, and career desires of themselves through a behavioral planning that can establish a professional and value system in accordance with them.

Protective Effects of an Ethanol Extract of Elaeagnus umbellata Leaves on α-MSH-induced Melanin Production in B16-F0 Cells and UVB-induced Damage in CCD-986sk Cells (보리수나무 잎 에탄올 추출물이 α-MSH 유도 B16-F0 세포의 멜라닌 생성 및 UVB 유도성 CCD-986sk 세포 손상에 미치는 효과)

  • Park, Se-Ho;Jhee, Kwang-Hwan;Yang, Seun-Ah
    • Journal of Life Science
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    • v.29 no.5
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    • pp.555-563
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    • 2019
  • This study was undertaken to investigate the effect of an ethanol extract of Elaeagnus umbellata leaves (EUL-EE) on skin-related biological activities. Previously, we have reported that gallic acid was the major phenolic compound in EUL-EE through quantitative analysis and that EUL-EE had an inhibitory effect against the proliferation of liver cancer HepG2 cells. In the present study, the inhibitory effects of EUL-EE on melanin production and tyrosinase activity in ${\alpha}$-melanocyte-stimulated hormone-stimulated B16-F0 cells were determined to assess the effects of EUL-EE on skin whitening. The anti-wrinkle effect using UVB-irradiated CCD-986sk cells was examined by the expression of type I procollagen and metalloproteinase (MMP)-1 release. The EUL-EE significantly decreased intracellular melanin production (33.0% inhibition at $100{\mu}g/ml$) when compared with untreated B16-F0 cells. Tyrosinase activities in the stimulated B16-F0 cells were also decreased by EUL-EE (47.8% inhibition at $100{\mu}g/ml$). The EUL-EE also dose-dependently increased the production of type I procollagen (up to 1.74-fold at $250{\mu}g/ml$) in CCD-986sk cells when compared with UVB-irradiated controls. EUL-EE showed no cytotoxicity at concentrations up to $500{\mu}g/ml$. In addition, EUL-EE at $10-500{\mu}g/ml$ inhibited the release of MMP-1 to the medium from UVB-irradiated CCD-986sk cells. Taken together, these observations indicate that EUL-EE has high potential for use as inner beauty and cosmetic materials due to its whitening and anti-wrinkle effects.

The history of awareness for ginseng in Vietnam (베트남의 고려인삼 인식에 대한 소고)

  • Ock, Soonjong
    • Journal of Ginseng Culture
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    • v.1
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    • pp.78-92
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    • 2019
  • Among the Southeast Asian countries, Vietnam had the most cultural exchange with Korea. Through Confusion and Chinese character, which were the common measure for two countries, Korea and Vietnam could communicate and share a sentiment with each other. Two countries showed an enormous interest in each other, and hence they could keep good relationship without official diplomatic relations. During the early 19th century, Korean Ginseng had gained tremendous popularity in Vietnam. Vietnamese believed the stamina of Minh Mang, who had 142 sons and daughters, originated from ginseng. Minh Mang bestowed ginseng on old courtiers for their contribution, which made them more loyal to the king. This tradition was inherited to the future generations, and soldiers who fought with Thai, Cambodia and France also received ginseng for their contribution. In other words, ginseng was the very important key for enforcement of patriotism. Due to the tradition, Korean ginseng has been considered as the premium ginseng in Vietnam. It is presumed that ginseng flew into Vietnam through 4 routes; 1) as an imperial gift from Chinese emperor, 2) by the Vietnamese ambassadors who went to Beijing for a tribute, 3) through private trade and 4) from Ryukyu or Japan, which were the hub of international trade. From 15th to 18th century, ambassadors from Chosun and Vietnam actively interacted in Beijing. Through their interchange, Vietnamese royal family could learn about the value of ginseng. The fact that there were many Shilhak scholars among the ambassadors from Joseon, such as Seo Geojeong and Seo Hosu who had profound knowledge of ginseng proves the theory. It is also possible that reputation of ginseng was already established in Vietnam during Silla period. Ko Byung, an bureaucrat from Tang Dynasty who ruled Vietnam for 10 years in middle 9th century and a friend of Choi Chiwon, might have delivered the knowledge of ginseng to the upper class in Vietnam. This hypothesis, however, should be proven by literatures. Hence, further research about the trace of Korean ginseng in Vietnamese history and literary works still remains to be done.

Development of HRM Markers Based on SNPs Identified from Next Generation Resequencing of Susceptible and Resistant Parents to Gummy Stem Blight in Watermelon (수박에서 덩굴마름병 감수성 및 저항성 양친에 대한 차세대 염기서열 재분석으로 탐색된 SNP 기반 HRM 분자표지 개발)

  • Lee, Eun Su;Kim, Jinhee;Hong, Jong Pil;Kim, Do-Sun;Kim, Minkyong;Huh, Yun-Chan;Back, Chang-Gi;Lee, Jundae;Lee, Hye-Eun
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.424-433
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    • 2018
  • Watermelon (Citrullus lanatus) is an economically important vegetable crop all over the world, which has functional compounds such as lycopene and citrulline. Gummy stem blight caused by Didymella bryoniae is one of the most devastative diseases in watermelon. Single nucleotide polymorphisms (SNPs), which are genetic variations occurring between individuals with respect to a single base, were often used to construct genetic linkage maps and develop molecular markers linked to a variety of horticultural traits and resistance to several diseases. In this study, we developed high-resolution melting (HRM) markers based on SNPs generated from NGS resequencing of two parents in watermelon. Plant materials were C. lanatus '920533' (female and susceptible parent), C. amarus 'PI 189225' (male and resistant parent), and their $F_1$ and $F_2$ progenies. A total of 13.6 Gbp ('920533') and 13.1 Gbp ('PI 189225') of genomic sequences were obtained using NGS analysis. A total of 6.09 million SNPs between '920533' and 'PI 189225' were detected, and 354,860 SNPs were identified as potential HRM primer sets. From these, a total of 330 primer sets for HRM analysis were designed. As a result, a total of 61 HRM markers that have polymorphic melting curves were developed. These HRM markers can be used for the construction of SNP-based linkage maps and for the analysis of quantitative trait loci (QTLs) related to gummy stem blight resistance.

Analysis of Monitoring Characteristics of Small Stream for TMDL (오염총량관리를 위한 소하천 모니터링 자료의 특성 분석)

  • Ha, Don-Woo;Park, Seung-Ho;Joo, Sungmin;Lee, Gi-Soon;Baek, Jong-Hun;Jung, Kang-Young;Lee, Youngjea;Kim, Kyunghyun;Kim, Young-Suk
    • Journal of the Korean Society for Environmental Technology
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    • v.19 no.6
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    • pp.503-513
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    • 2018
  • In order to continuous watershed management and improve water quality at Yeong-san river system, we analyzed and evaluated data on the monitoring of small stream in city and county boundaries within the watershed. In-period monitoring is estimated to be more frequent in the second quarter than the first quarter, so it should be considered when evaluating the target water quality by setting the target water quality. A small stream in the Yeong-san river system has higher concentration in the downstream area than the upstream area. As a result of calculating the load of the measuring point, Y.b B3(Pungyeongjeongcheon) and Y.b E1(Sampocheon) were high. The result of correlation analysis by monitoring point in order to evaluate the correlation between BOD and T-P items, BOD was highly correlated with COD and TOC, and was affected by emission of pollutants related to organic matter. T-P was highly correlated with SS and COD, and was affected by rainfall. This study will provide basic data and direction for designing efficient and scientific method for water quality management by analyzing accumulated water quality data by conducting long-term monitoring.

A Study on the Reasonable Measurement Point of Root Collar Diameter of Landscape Trees in Korea (한국 조경수목 근원직경 측정의 합리적 위치 설정에 대한 연구)

  • Han, Yong-Hee;Kim, Hwa-Jeong;Kim, Do-Gyun
    • Journal of the Korean Institute of Landscape Architecture
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    • v.49 no.5
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    • pp.59-70
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    • 2021
  • This study was to investigate the measurement point of root collar diameter of landscape trees in Korea. It may contribute to avoiding disputes caused by the difference in measurement criteria of root collar diameter of landscape trees between tree growers and constructors. The difference between landscape trees' root collar diameter measurement point was 3.59cm from 6cm underground to the surface and 1.35cm from 0cm to 6cm above ground. The source root collar diameter measurement point difference was larger in the basement than in the ground. The standard deviation of the root collar diameter of the landscape tree was 0.64 from 6cm underground to the surface, and the difference in standard deviation from 0cm to 6cm above ground was 0.16. The difference by measurement point of the root collar diameter was larger in the basement than in the ground. It has been proposed to set the reasonable measurement point of the landscaping tree root collar diameter at the inflection point where the standard deviation of the tree trunk diameter is the smallest in line with the size change of the standard for each root collar diameter measurement point. By tree species, Cornus officinalis Siebold & Zucc. 18cm above the ground, Chionanthus retusus Lindl. & Paxton. 12cm above the ground, Zelkova serrata (Thunb.) Makino. 12cm above the ground, Celtis sinensis Pers. 12cm above the ground, Styrax japonicus Siebold & Zucc. 10 cm above the ground, Cornus officinalis Siebold & Zucc. 10cm above the ground, Acer palmatum Thunb. ex Murray. 6cm above the ground, Ilex rotunda Thunb. 6cm above the ground, Quercus myrsmaefolia Blume. 4cm above the ground, Lagerstroemia indica L. 2cm above the ground The above heights were shown as reasonable measurement points. The difference by landscape tree root collar diameter measurement site showed that the standard deviation was small throughout the tree species, and the reasonable average measurement point with a stable slope of the deviation was 12cm or more on average. It can be said that the reasonable measurement point of the root collar diameter of a landscape tree is set at an average of 12cm above the ground. However, recognizing 30cm, which is a familiar ruler(尺) in traditional practices, is quick, It was recommended to measure at the height of 30cm from the surface for a reasonable measurement point of the root collar diameter of a landscape tree, for the uniformity of measurement standards.